ILF2 (NM_004515) Human Untagged Clone

CAT#: SC126956

ILF2 (untagged)-Human interleukin enhancer binding factor 2, 45kDa (ILF2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ILF2
• Synonyms: NF45; PRO3063
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC126956 sequence for NM_004515 edited (data generated by NextGen Sequencing)
  ATGAGGGGTGACAGAGGCCGTGGTCGTGGTGGGCGCTTTGGTTCCAGAGGAGGCCCAGGA
  GGAGGGTTCAGGCCCTTTGTACCACATATCCCATTTGACTTCTATTTGTGTGAAATGGCC
  TTTCCCCGGGTCAAGCCAGCACCTGATGAAACTTCCTTCAGTGAGGCCTTGCTGAAGAGG
  AATCAGGACCTGGCTCCCAATTCTGCTGAACAGGCATCTATCCTTTCTCTGGTGACAAAA
  ATAAACAATGTGATTGATAATCTGATTGTGGCTCCAGGGACATTTGAAGTGCAAATTGAA
  GAAGTTCGACAGGTGGGATCCTATAAAAAGGGGACAATGACTACAGGACACAATGTGGCT
  GACCTGGTGGTGATACTCAAGATTCTGCCAACGTTGGAAGCTGTTGCTGCCCTGGGGAAC
  AAAGTCGTGGAAAGCCTAAGAGCACAGGATCCTTCTGAAGTTTTAACCATGCTGACCAAC
  GAAACTGGCTTTGAAATCAGTTCTTCTGATGCTACAGTGAAGATTCTCATTACAACAGTG
  CCACCCAATCTTCGAAAACTGGATCCAGAACTCCATTTGGATATCAAAGTATTGCAGAGT
  GCCTTAGCAGCCATCCGACATGCCCGCTGGTTCGAGGAAAATGCTTCTCAGTCCACAGTT
  AAAGTTCTCATCAGACTACTGAAGGACTTGAGGATTCGTTTTCCTGGCTTTGAGCCCCTC
  ACACCCTGGATCCTTGACCTACTAGGCCATTATGCTGTGATGAACAACCCCACCAGACAG
  CCTTTGGCCCTAAACGTTGCATACAGGCGCTGCTTGCAGATTCTGGCTGCAGGACTGTTC
  CTGCCAGGTTCAGTGGGTATCACTGACCCCTGTGAGAGTGGCAACTTTAGAGTACACACA
  GTCATGACCCTAGAACAGCAGGACATGGTCTGCTATACAGCTCAGACTCTCGTCCGAATC
  CTCTCACATGGTGGCTTTAGGAAGATCCTTGGCCAGGAGGGTGATGCCAGCTATCTTGCT
  TCTGAAATATCTACCTGGGATGGAGTGATAGTAACACCTTCAGAAAAGGCTTATGAGAAG
  CCACCAGAGAAGAAGGAAGGAGAGGAAGAAGAGGAGAATACAGAAGAACCACCTCAAGGA
  GAGGAAGAAGAAAGCATGGAAACTCAGGAGTGA
  
  Clone variation with respect to NM_004515.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004515 unedited
  AGATTTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGGAAGGGGCGGTTG
  GTGCGGCCTCCATTGTTCGTGTTTTAAGGCGCCATGAGGGGTGACAGAGGCCGTGGTCGT
  GGTGGGCGCTTTGGTTCCAGAGGAGGCCCAGGAGGAGGGTTCAGGCCCTTTGTACCACAT
  ATCCCATTTGACTTCTATTTGTGTGAAATGGCCTTTCCCCGGGTCAAGCCAGCACCTGAT
  GAAACTTCCTTCAGTGAGGCCTTGCTGAAGAGGAATCAGGACCTGGCTCCCAATTCTGCT
  GAACAGGCATCTATCCTTTCTCTGGTGACAAAAATAAACAATGTGATTGATAATCTGATT
  GTGGCTCCAGGGACATTTGAAGTGCAAATTGAAGAAGTTCGACAGGTGGGATCCTATAAA
  AAGGGGACAATGACTACAGGACACAATGTGGCTGACCTGGTGGTGATACTCAAGATTCTG
  CCAACGTTGGAAGCTGTTGCTGCCCTGGGGAACAAAGTCGTGGAAAGCCTAAGAGCACAG
  GATCCTTCTGAAGTTTTAACCATGCTGACCAACGAAACTGGCTTTGAAATCAGTTCTTCT
  GATGCTACAGTGAAGATTCTCATTACAACAGTGCCACCCAATCTTCGAAAACTGGATCCA
  GAACTCCATTTGGATATCAAAGTATTGGCAGAGTGCCTTAGCAGCCATCCGACATGCCCG
  CTGGTTCGAGGAAAATGCTTCTCAGTCCCAGNTTAAAGTTCTCATCAGACTACTGAAGGA
  CTNGAAGGATNCGNTNTCCTGGCTTTGAGCCCCTCCACCCCTGGATCCTTTGACCTACTA
  NGNCATTATGCTGTGATGAACNACCCCACCAGAAGNCTTTGGCCCCCTAACGTGATACNA
  GCGCTGCTTGCAGATCTGCTGGCANGACTGTCCTGCACANGTTCAAGGGGATCACATGAN
  CTGGGGAGAAGGCACTTTTA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004515 unedited
  GTACCGCGGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTGCCCTACTAGGAAG
  TACTTTAATAGTTTTTCTTAGAAAAAAAATTTCCAGACACTTAACATTTCACAACATTTC
  AACAGCAAAGTATTAGTTGAGAGAGGGGTTTTCAGGAGTTGGAGATTATAGAATATTAGG
  AAGAAATGTTGGTATCCTCCATTATAGATGGATGGCATAGGTCACAAATGGGAGACTGGC
  AGCTAAGCCAATATCAAAACCCAGTGGAATGACACTTCTATGGAGTTTACTTTTCTTCCT
  GCTATCTTCCCTATCCCACGGAAATGTCTGTCACCATGTAAAGCCCAGTAGCAGGCAGCT
  TAGGCTCCAGTCTTCCCCCTTGGGTAGGAAAAGGAGTGAAGAGAATGTCACTCCTGAGTT
  TCCATGCTTTCTTCTTCCTCTCCTTGAGGTGGGTCTTCTGTATTCTCCTCTTCTTCCTCT
  CCTTCCTTCTTCTCTGGTGGCTTCTCATAAGCCTTTTCTGAAGGTGTTACTATCACTCCA
  TCCCAGGTAGATATTTCAGAAGCAAGATAGCTGGCATCACCCTCCTGGCCAAGGATCTTC
  CTAAAGCCACCATGTGAGAGGATTCGGACGAGAGTCTGAGCTGTATAGCAGACCATGTCC
  TGCTGTTCTAGGGTCATGACTGTGTGTACTCTAAAGTTGCCACTCTCACAGGGGTCAGTG
  ATACCCACTGAACCTGGCANGAACAGTCCTGCAGCCAGAATCTGCAAGCAGCGCCTGTAT
  GCAACGTTTAGGGCCAAAGGCTGTCTGGTGGGGNTNGTTCATCACAGCATAATGGCCTAN
  TANGTCAGGGATCCANGGTGTGAGGGGCTCANAGCCANGAAAACGAATCCTCCAGTCCTT
  CAGTAGTCTGT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004515
• Insert Size: 1650 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004515.2, NP_004506.2
• RefSeq Size: 1637 bp
• RefSeq ORF: 1173 bp
• Locus ID: 3608
• Cytogenetics: 1q21.3
• Domains: DZF
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: 'The protein encoded by this gene is a transcription factor required for T-cell expression of the interleukin 2 gene. It also binds RNA and is an essential component for encapsidation and protein priming of hepatitis B viral polymerase. The encoded 45 kDa protein (NF45, ILF2) forms a complex with the 90 kDa interleukin enhancer-binding factor 3 (NF90, ILF3), and this complex has been shown to affect the redistribution of nuclear mRNA to the cytoplasm, to repair DNA breaks by nonhomologous end joining, and to negatively regulate the microRNA processing pathway. Knockdown of NF45 or NF90 protein retards cell growth, possibly by inhibition of mRNA stabilization. Alternative splicing results in multiple transcript variants. Related pseudogenes have been found on chromosomes 3 and 14. [provided by RefSeq, Dec 2014]'
  Transcript Variant: This variant (1) encodes the longer isoform (1).