ATP5F1C (NM_005174) Human Untagged Clone

CAT#: SC126967

ATP5C1 (untagged)-Human ATP synthase, H+ transporting, mitochondrial F1 complex, gamma polypeptide 1 (ATP5C1), nuclear gene encoding mitochondrial protein, transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ATP5F1C
• Synonyms: ATP5C; ATP5C1; ATP5CL1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC126967 sequence for NM_005174 edited (data generated by NextGen Sequencing)
  ATGTTCTCTCGCGCGGGTGTCGCTGGGCTGTCGGCCTGGACCTTGCAGCCGCAATGGATT
  CAAGTTCGAAATATGGCAACTTTGAAAGATATCACCAGGAGACTAAAGTCCATCAAAAAC
  ATCCAGAAAATTACCAAGTCTATGAAAATGGTAGCGGCAGCAAAATATGCCCGAGCTGAG
  AGAGAGCTGAAACCAGCTCGAATATATGGATTGGGATCTTTAGCTCTGTATGAAAAAGCT
  GATATCAAGGGGCCTGAAGACAAGAAGAAACACCTCCTTATTGGTGTGTCCTCAGATCGA
  GGACTGTGTGGTGCTATTCATTCCTCCATTGCTAAACAGATGAAAAGCGAGGTTGCTACA
  CTAACAGCAGCTGGGAAAGAAGTTATGCTTGTTGGAATTGGTGACAAAATCAGAGGCATA
  CTTTATAGGACTCATTCTGACCAGTTTCTGGTGGCATTCAAAGAAGTGGGAAGAAAGCCC
  CCCACTTTTGGAGATGCGTCAGTCATTGCCCTTGAATTACTAAATTCTGGATATGAATTT
  GATGAAGGCTCCATCATCTTTAATAAATTCAGGTCTGTCATCTCCTATAAGACAGAAGAA
  AAGCCCATCTTTTCCCTTAATACCGTTGCAAGTGCTGACAGCATGAGTATCTATGACGAT
  ATTGATGCTGACGTGCTGCAAAATTACCAAGAATACAATCTGGCCAACATCATCTACTAC
  TCTCTGAAGGAGTCCACCACTAGTGAGCAGAGTGCCAGGATGACAGCCATGGACAATGCC
  AGCAAGAATGCTTCTGAGATGATTGACAAATTGACATTGACATTCAACCGTACCCGCCAA
  GCTGTCATCACAAAAGAGTTGATTGAAATTATCTCTGGTGCTGCAGCTCTGTAA
  
  Clone variation with respect to NM_005174.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005174 unedited
  CATTTGTATACGACNACTATAGGGCGGCCGCGATTCGGCACGAGGCCGACCTTCAGCAGG
  GCTGTGGCTACCATGTTCTCTCGCGCGGGTGTCGCTGGGCTGTCGGCCTGGACCTTGCAG
  CCGCAATGGATTCAAGTTCGAAATATGGCAACTTTGAAAGATATCACCAGGAGACTAAAG
  TCCATCAAAAACATCCAGAAAATTACCAAGTCTATGAAAATGGTAGCGGCAGCAAAATAT
  GCCCGAGCTGAGAGAGAGCTGAAACCAGCTCGAATATATGGATTGGGATCTTTAGCTCTG
  TATGAAAAAGCTGATATCAAGGGGCCTGAAGACAAGAAGAAACACCTCCTTATTGGTGTG
  TCCTCAGATCGAGGACTGTGTGGTGCTATTCATTCCTCCATTGCTAAACAGCTGAAAAGC
  GAGGTTGCTACACTAACAGCAGCTGGGAAAGAAGTTATGCTTGTTGGAATTGGTGACAAA
  ATCAGAGGCATACTTTATAGGACTCATTCTGACCAGTTTCTGGTGGCATTCAAAGAAGTG
  GGAAGAAAGCCCCCCACTTTTGGAGATGCGTCAGTCATTGCCCTTGAATTACTAAATTCT
  GGATATGAATTTGATGAAGGCTCCATCATCTTTAATAAATTCAGGTCTGTCATCTCCTAT
  AAGACAGAGAAAAGCCCCATCTTTCCCTTATACCNNGTGCAGTGCTGACAGCATGAGTAT
  CTATGACGATATTGATGCTGACGTGCTGCAAAATACCAAGAATACATCTGGGCCACATCA
  TCTACTACTCTCTGGAGGAGTCCACCACTAGTGAGCAGAGTGCCCAGATGACAGCCATGA
  CCATGCCAAGCAGAATGCTTCTGAGAGATTGACAATTGACATGACATTCACCGTACCCGC
  CAGCTGTATCACAAAGAGTGATGAT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005174 unedited
  CCGCAATCTAGAATCGAGTTCTTTTTTTTTTTTTTTATTTTAAGCTGCTTATTTTAAGAT
  AATTTACAAATATCTTGCTGTCTTCAGTAATTCAAATAATGGAGGAACGGTTTCTTCGGA
  CAAAGGCAGCAGTAAGCTAAAAACAAAATCAACTGGCTGAATTTTCCTTCTTTACCTCTT
  GTCTGAGGATGGAACTTGATTTTCATTAATCCAGAGCTGCAGCACCAGAGATAATTTCAA
  TCAACTCTTTTGTGATGACAGCTTGGCGGGTACGGTTGAATGTCAATGTCAATTTGTCAA
  TCATCTCAGAAGCATTCTTGCTGGCATTGTCCATGGCTGTCATCCTGGCACTCTGCTCAC
  TAGTGGTGGACTCCTTCAGAGAGTAGTAGATGATGTTGGCCAGATTGTATTCTTGGTAAT
  TTTGCAGCACGTCAGCATCAATATCGTCATAGATACTCATGCTGTCAGCACTTGCAACGG
  TATTAAGGGAAAAGATGGGCTTTTCTTCTGTCTTATAGGAGATGACAGACCTGAATTTAT
  TAAAGATGATGGAGCCTTCATCAAATTCATATCCAGAATTTAGTAATTCAAGGGCAATGA
  CTGACGCATCTCCAAAAGTGGGGGGCTTTCTTCCCACTTCTCTGAATGCCACCAGAAACT
  GGTCAGAATGAGTCCTATAAAGTATGCCTCTGATTCTGTCACCAATTCCAACAAGCATAA
  CTTCTTTCCCAGCTGCTGTTAGAGTAGCACCTCGCCTTTCATCTGTTTAGCATTGGAGGA
  ATGATTAGCACCCACAGTCCTCGATCTGAGGACACACCATTAAGAGGTGTTTCTCCTGCC
  TTCAGCCCCTTGAATCAGCTCTTCATACGAGCTTAGATCCCATCCATTATTCGAGCTGGT
  TCAGCTCCTCTCAGCTCGGCATATTTGCTGCGCTACATTTATAAACTGGAATTCCGGATG
  TTTGATGACTTAGCTCCGCGAATCTN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005174
• Insert Size: 1110 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005174.2, NP_005165.1
• RefSeq Size: 1125 bp
• RefSeq ORF: 894 bp
• Locus ID: 509
• Cytogenetics: 10p14
• Domains: ATP-synt
• Protein Pathways: Alzheimer's disease, Huntington's disease, Metabolic pathways, Oxidative phosphorylation, Parkinson's disease
• Gene Summary: 'This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the gamma subunit of the catalytic core. Alternatively spliced transcript variants encoding different isoforms have been identified. This gene also has a pseudogene on chromosome 14. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (2) lacks a segment in the coding region compared to variant 1. The resulting isoform (H), also known as a heart type isoform, contains a shorter and distinct C-terminus compared to isoform L.