Microsomal Glutathione S transferase 1 (MGST1) (NM_145791) Human Untagged Clone

CAT#: SC126996

MGST1 (untagged)-Human microsomal glutathione S-transferase 1 (MGST1), transcript variant 1c

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MGST1
• Synonyms: GST12; MGST; MGST-I
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC126996 sequence for NM_145791 edited (data generated by NextGen Sequencing)
  ATGGTTGACCTCACCCAGGTAATGGATGATGAAGTATTCATGGCTTTTGCATCCTATGCA
  ACAATTATTCTTTCAAAAATGATGCTTATGAGTACTGCAACTGCATTCTATAGATTGACA
  AGAAAGGTTTTTGCCAATCCAGAAGACTGTGTAGCATTTGGCAAAGGAGAAAATGCCAAG
  AAGTATCTTCGAACAGATGACAGAGTAGAACGTGTACGCAGAGCCCACCTGAATGACCTT
  GAAAATATTATTCCATTTCTTGGAATTGGCCTCCTGTATTCCTTGAGTGGTCCCGACCCC
  TCTACAGCCATCCTGCACTTCAGACTATTTGTCGGAGCACGGATCTACCACACCATTGCA
  TATTTGACACCCCTTCCCCAGCCAAATAGAGCTTTGAGTTTTTTTGTTGGATATGGAGTT
  ACTCTTTCCATGGCTTACAGGTTGCTGAAAAGTAAATTGTACCTGTAA
  
  Clone variation with respect to NM_145791.1
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_145791 unedited
  TTGATATTTGTATACGACTTACTATAGGGCGGCCGCGAAATTCGCACGAGCCTCCTCCTC
  GGCCTCACCATTCCAGACCAAAATTGAAAAAAGGTTGACCTCACCCAGGTAATGGATGAT
  GAAGTATTCATGGCTTTTGCATCCTATGCAACAATTATTCTTTCAAAAATGATGCTTATG
  AGTACTGCAACTGCATTCTATAGATTGACAAGAAAGGTTTTTGCCAATCCAGAAGACTGT
  GTAGCATTTGGCAAAGGAGAAAATGCCAAGAAGTATCTTCGAACAGATGACAGAGTAGAA
  CGTGTACGCAGAGCCCACCTGAATGACCTTGAAAATATTATTCCATTTCTTGGAATTGGC
  CTCCTGTATTCCTTGAGTGGTCCCGACCCCTCTACAGCCATCCTGCACTTCAGACTATTT
  GTCGGAGCACGGATCTACCACACCATTGCATATTTGACACCCCTTCCCCAGCCAAATAGA
  GCTTTGAGTTTTTTTGTTGGATATGGAGTTACTCTTTCCATGGCTTACAGGTTGCTGAAA
  AGTAAATTGTACCTGTAAAGAAAATCATACAACTCAGCATCCAGTTGGCTTTTTAAGAAT
  TCTGTACTTCCAATGTATAATGAATACTTTCTTANATTTANGATTGTAGGGTACGAGGGC
  GAGCACNAAGAGAATTATGAAACGTGGGGCTAAACCTCATTTCTGAATAATCTAGCATCT
  GCCAATATCCTGTATTTCTTACTTTTACANTTTGGATTAAAAAATCTAACATAGCTAATT
  CTTAAAGTCTTTCTGTCTGATTTTTAAAGTACTTTCTTTATAATTTGGATCATGTTATGA
  TTTGTAACATTCACACAACACCTCACTTTTGAATCTATAAAAGAATCGCACGTATGAGAA
  ACCTATATCTCAATACTGCTGAANCAGACATGAATAAAGATTTCTAAGATGAAAAAAAAA
  AAAAAAAAACTCACTCTAGATTGCGGCG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_145791
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145791.1, NP_665734.1
• RefSeq Size: 987 bp
• RefSeq ORF: 468 bp
• Locus ID: 4257
• Cytogenetics: 12p12.3
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Drug metabolism - cytochrome P450, Glutathione metabolism, Metabolism of xenobiotics by cytochrome P450
• Gene Summary: 'The MAPEG (Membrane Associated Proteins in Eicosanoid and Glutathione metabolism) family consists of six human proteins, two of which are involved in the production of leukotrienes and prostaglandin E, important mediators of inflammation. Other family members, demonstrating glutathione S-transferase and peroxidase activities, are involved in cellular defense against toxic, carcinogenic, and pharmacologically active electrophilic compounds. This gene encodes a protein that catalyzes the conjugation of glutathione to electrophiles and the reduction of lipid hydroperoxides. This protein is localized to the endoplasmic reticulum and outer mitochondrial membrane where it is thought to protect these membranes from oxidative stress. Several transcript variants, some non-protein coding and some protein coding, have been found for this gene. [provided by RefSeq, May 2012]'
  Transcript Variant: This variant (3, also called 1c) differs in the 5' UTR compared to variant 1. Variants 1, 2, 3, 4, and 5 all encode the same isoform (a).