UBE2I (NM_194260) Human Untagged Clone

CAT#: SC127053

UBE2I (untagged)-Human ubiquitin-conjugating enzyme E2I (UBE2I), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: UBE2I
• Synonyms: C358B7.1; P18; UBC9
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_194260, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTCGGGGATCGCCCTCAGCAGACTCGCCCAGGAGAGGAAAGCATGGAGGAAAGACCACCCATTTGGTT
  TCGTGGCTGTCCCAACAAAAAATCCCGATGGCACGATGAACCTCATGAACTGGGAGTGCGCCATTCCAGG
  AAAGAAAGGGACTCCGTGGGAAGGAGGCTTGTTTAAACTACGGATGCTTTTCAAAGATGATTATCCATCT
  TCGCCACCAAAATGTAAATTCGAACCACCATTATTTCACCCGAATGTGTACCCTTCGGGGACAGTGTGCC
  TGTCCATCTTAGAGGAGGACAAGGACTGGAGGCCAGCCATCACAATCAAACAGATCCTATTAGGAATACA
  GGAACTTCTAAATGAACCAAATATCCAAGACCCAGCTCAAGCAGAGGCCTACACGATTTACTGCCAAAAC
  AGAGTGGAGTACGAGAAAAGGGTCCGAGCACAAGCCAAGAAGTTTGCGCCCTCATAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_194260 unedited
  ATATCCCCCGCCCGTTGACGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAG
  CAGAGTTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGC
  GAATTCGGCACGAGGACAAAGGGAAGCGCCGCCGCCGCCGCCACCTGTCCGCTACGCTCG
  CCGGGGCTGCGGCCGCCCGAGGGACTTTGAACATGTCGGGGATCGCCCTCAGCAGACTCG
  CCCAGGAGAGGAAAGCATGGAGGAAAGACCACCCATTTGGTTTCGTGGCTGTCCCAACAA
  AAAATCCCGATGGCACGATGAACCTCATGAACTGGGAGTGCGCCATTCCAGGAAAGAAAG
  GGACTCCGTGGGAAGGAGGCTTGTTTAAACTACGGATGCTTTTCAAAGATGAGTATCCAT
  CTTCGCCACCAAAATGTAAATTCGAACCACCATTATTTCACCCGAATGTGTACCCTTCGG
  GGACAGTGTGCCTGTCCATCTTAGAGGAGGACAAGGACTGGAGGCCAGCCATCACAATCA
  AACAGATCCTATTAGGAATACAGGAACTTCTAAATGAACCAAATATCCAAGACCCAGCTC
  AAGCAGAGGCCTACACGATTTACTGCCAAAACAGAGTGGAGTACTAGAAAAGGGTCCGAG
  CACAAGCCAAGAAGTTTGCGCCCTCATAAGCAGCGACCTTGTGGCATCGTCAGAAGGAAG
  GGACTTGGTTTGGCAAGAACTTGTTTACGACATTTTTGCAAATCTAAAGTTGCTCCATAC
  AATGACTAGTCACCTGNGGGGGGATGGGGCGGGGCGCCATCTTCCATTGCCGCCGCGGGA
  TGTGCGGTCTCGATTCGCTGAATTGCCTGATACATACAGGGNCTCTTNCTTCGGNCTATT
  GNATTATTGACTGGTATGTACAACTCGCTTTTAGTTTACGTATGATGGCAGTATTTCACT
  GCNTGTAAAGTATAACGTTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_194260
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_194260.1, NP_919236.1
• RefSeq Size: 1144 bp
• RefSeq ORF: 477 bp
• Locus ID: 7329
• Cytogenetics: 16p13.3
• Protein Pathways: Ubiquitin mediated proteolysis
• Gene Summary: 'The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (3) differs in the 5' UTR, as compared to variant 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.