CKS2 (NM_001827) Human Untagged Clone

CAT#: SC127099

CKS2 (untagged)-Human CDC28 protein kinase regulatory subunit 2 (CKS2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CKS2
• Synonyms: CKSHS2
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC127099 sequence for NM_001827 edited (data generated by NextGen Sequencing)
  ATGGCCCACAAGCAGATCTACTACTCGGACAAGTACTTCGACGAACACTACGAGTACCGG
  CATGTTATGTTACCCAGAGAACTTTCCAAACAAGTACCTAAAACTCATCTGATGTCTGAA
  GAGGAGTGGAGGAGACTTGGTGTCCAACAGAGTCTAGGCTGGGTTCATTACATGATTCAT
  GAGCCAGAACCACATATTCTTCTCTTTAGACGACCTCTTCCAAAAGATCAACAAAAATGA
  
  Clone variation with respect to NM_001827.1
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001827 unedited
  NTTATTTCCCCGCCCGTTGNCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATA
  AGCAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCC
  GCGAATTCGGCACGAGGCGAGTTGTTGCCTGGGCTGGACGTGGTTTTGTCTGCTGCGCCC
  GCTCTTCGCGCTCTCGTTTCATTTTCTGCAGCGCGCCAGCAGGATGGCCCACAAGCAGAT
  CTACTACTCGGACAAGTACTTCGACGAACACTACGAGTACCGGCATGTTATGTTACCCAG
  AGAACTTTCCAAACAAGTACCTAAAACTCATCTGATGTCTGAAGAGGAGTGGAGGAGACT
  TGGTGTCCAACAGAGTCTAGGCTGGGTTCATTACATGATTCATGAGCCAGAACCACATAT
  TCTTCTCTTTAGACGACCTCTTCCAAAAGATCAACAAAAATGAAGTTTATCTGGGGATCG
  TCAAATCTTTTTCAAATTTAATGTATATGTGTATATAAGGTAGTATTCAGTGAATACTTG
  AGAAATGTACAAATCCTTCATCCATACCTGTGCATGAGCTGTATTCTTCACAGCAACAGA
  GCTCAGTTAAATGCAACTGCAAGTAGGTTACTGTAAGATGTTTAAGATAAAAGTTCTTCC
  AGTCAGTTNTTCTCTTAAGTGCCTGTTTGAGTTTACTGAAACAGTTTACTTTTGTTCAAA
  TAAAGTTGTATGTTGCATTTAAAAAAAAAAAAAAAAAACTCGACTCTAGATTGCGGNCGC
  GGTCATAGCTGGNTCCCTGACAGATCCCGGGTGGCATCCCTGTGACCCTNCCCAGTGCCC
  TCTCTGGCCCTGNNNAGTGCCACTNCAGTGCCCACCAGCCNTTGTCTATAAAAATAAAGT
  GCATCATTTTGCTGACTAGA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001827
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001827.1, NP_001818.1
• RefSeq Size: 627 bp
• RefSeq ORF: 240 bp
• Locus ID: 1164
• Cytogenetics: 9q22.2
• Domains: CKS
• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Gene Summary: 'CKS2 protein binds to the catalytic subunit of the cyclin dependent kinases and is essential for their biological function. The CKS2 mRNA is found to be expressed in different patterns through the cell cycle in HeLa cells, which reflects specialized role for the encoded protein. [provided by RefSeq, Jul 2008]'