D4 (ARHGDIB) (NM_001175) Human Untagged Clone

CAT#: SC127108

ARHGDIB (untagged)-Human Rho GDP dissociation inhibitor (GDI) beta (ARHGDIB)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ARHGDIB
• Synonyms: D4; GDIA2; GDID4; Ly-GDI; LYGDI; RAP1GN1; RhoGDI2
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC127108 sequence for NM_001175 edited (data generated by NextGen Sequencing)
  ATGACTGAAAAAGCCCCAGAGCCACATGTGGAGGAGGATGACGATGATGAGCTGGACAGC
  AAGCTCAATTATAAGCCTCCACCACAGAAGTCCCTGAAAGAGCTGCAGGAAATGGACAAA
  GATGATGAGAGTCTAATTAAGTACAAGAAAACGCTGCTGGGAGATGGTCCTGTGGTGACA
  GATCCGAAAGCCCCCAATGTCGTTGTCACCCGGCTCACCCTGGTTTGTGAGAGTGCCCCG
  GGACCAATCACCATGGACCTTACTGGAGATCTGGAAGCCCTCAAAAAGGAAACCATTGTG
  TTAAAGGAAGGTTCTGAATATAGAGTCAAAATTCACTTCAAAGTGAACAGGGATATTGTG
  TCAGGCCTGAAATACGTTCAGCACACCTACAGGACTGGGGTGAAAGTGGATAAAGCAACA
  TTTATGGTTGGCAGCTATGGACCTCGGCCTGAGGAGTATGAGTTCCTCACTCCAGTTGAG
  GAGGCTCCCAAGGGCATGCTGGCCCGAGGCACGTACCACAACAAGTCCTTCTTCACCGAC
  GATGACAAGCAAGACCACCTCAGCTGGGAGTGGAACCTGTCGATTAAGAAGGAGTGGACA
  GAATGA
  
  Clone variation with respect to NM_001175.4
  42 t=>c;504 g=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001175 unedited
  TTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGAGACGTGAAGCACTG
  AATAAATAGATCAGAATGACTGAAAAAGCCCCAGAGCCACATGTGGAGGAGGATGACGAT
  GATGAGCTGGACAGCAAGCTCAATTATAAGCCTCCACCACAGAAGTCCCTGAAAGAGCTG
  CAGGAAATGGACAAAGATGATGAGAGTCTAATTAAGTACAAGAAAACGCTGCTGGGAGAT
  GGTCCTGTGGTGACAGATCCGAAAGCCCCCAATGTCGTTGTCACCCGGCTCACCCTGGTT
  TGTGAGAGTGCCCCGGGACCAATCACCATGGACCTTACTGGAGATCTGGAAGCCCTCAAA
  AAGGAAACCATTGTGTTAAAGGAAGGTTCTGAATATAGAGTCAAAATTCACTTCAAAGTG
  AACAGGGATATTGTGTCAGGCCTGAAATACGTTCAGCACACCTACAGGACTGGGGTGAAA
  GTGGATAAAGCAACATTTATGGTTGGCAGCTATGGACCTCGGCCTGAGGAGTATGAGTTC
  CTCACTCCAGTTGAGGAGGCTCCCAAGGGCATGCTGGCCCGAGGCACGTACCACAACAAG
  TCCTTCTTCACCGACGATGACAAGCAAGACCACCTCAGCTGGGAGTGGAACCTGTCGATT
  AAGAAAGAGTGGACAGAATGAATGCATCCACCCCTTTCCCCACCGTTGCCACCTGNAAGA
  AATCTCTCAGGGCGTGTCAGCACCCTGTCCCTTCTCCCTGTCACAGCTGGGTCCCTCTTC
  ACACTGCCACATTNCTTATGATGCATCTTTNCCACCTGTCACTCACGTGGTCCTANAAAC
  AGAGCTAAANCGGNCTTNCACCACTGCTCCTTGATCTNCATAGGNCNATCTTCCAGTCTC
  ANCTAGACACATCATTATAATTCCTGGCTACCCTATCAGCACTAAGCCCAAAATGGGCAT
  ATACTAAGGCTTNGACTAGTNNCGANTCATCATGCTAATTCTTTGGGTGTGCTGCCAAGG
  CN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001175
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001175.4, NP_001166.3
• RefSeq Size: 1216 bp
• RefSeq ORF: 606 bp
• Locus ID: 397
• Cytogenetics: 12p12.3
• Domains: Rho_GDI
• Protein Families: Druggable Genome
• Protein Pathways: Neurotrophin signaling pathway
• Gene Summary: 'Members of the Rho (or ARH) protein family (see MIM 165390) and other Ras-related small GTP-binding proteins (see MIM 179520) are involved in diverse cellular events, including cell signaling, proliferation, cytoskeletal organization, and secretion. The GTP-binding proteins are active only in the GTP-bound state. At least 3 classes of proteins tightly regulate cycling between the GTP-bound and GDP-bound states: GTPase-activating proteins (GAPs), guanine nucleotide-releasing factors (GRFs), and GDP-dissociation inhibitors (GDIs). The GDIs, including ARHGDIB, decrease the rate of GDP dissociation from Ras-like GTPases (summary by Scherle et al., 1993 [PubMed 8356058]).[supplied by OMIM, Dec 2010]'