CTBP2 (NM_001329) Human Untagged Clone

CAT#: SC127174

CTBP2 (untagged)-Human C-terminal binding protein 2 (CTBP2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CTBP2
• Synonyms: C-terminal binding protein 2; OTTHUMP00000020699; OTTHUMP00000020701; ribeye
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC127174 sequence for NM_001329 edited (data generated by NextGen Sequencing)
  ATGGCCCTTGTGGATAAGCACAAAGTCAAGAGACAGCGATTGGACAGAATTTGTGAAGGT
  ATCCGCCCCCAGATCATGAACGGCCCCCTGCACCCCCGCCCCCTGGTGGCGCTGCTGGAC
  GGCCGCGACTGCACTGTGGAGATGCCCATCCTGAAGGACCTGGCCACTGTGGCCTTCTGT
  GACGCGCAGTCGACGCAGGAAATCCACGAGAAGGTTCTAAACGAAGCCGTGGGCGCCATG
  ATGTACCACACCATCACCCTCACCAGGGAGGACCTGGAGAAGTTCAAGGCCCTGAGAGTG
  ATCGTGCGGATAGGCAGTGGCTATGACAACGTGGACATCAAGGCTGCCGGCGAGCTCGGA
  ATTGCCGTGTGCAACATCCCGTCTGCAGCCGTGGAAGAGACAGCGGACTCTACCATCTGC
  CACATCCTCAACCTGTACCGGAGGAACACGTGGCTGTACCAGGCACTGCGGGAAGGCACG
  CGGGTTCAGAGCGTGGAGCAGATCCGCGAGGTGGCCTCGGGAGCGGCCCGCATCCGTGGG
  GAGACGCTGGGCCTCATTGGCTTTGGTCGCACGGGGCAGGCGGTTGCAGTTCGAGCCAAG
  GCCTTTGGATTCAGCGTCATATTTTATGACCCCTACTTGCAGGATGGGATCGAGCGGTCC
  CTGGGCGTGCAGAGGGTCTACACCCTGCAGGATTTGCTGTATCAGAGCGACTGCGTCTCC
  TTGCACTGCAATCTCAACGAACATAACCACCACCTCATCAATGACTTTACCATAAAGCAG
  ATGAGGCAGGGAGCATTCCTTGTGAACGCAGCCCGTGGCGGCCTGGTGGACGAGAAAGCC
  TTAGCACAAGCCCTCAAGGAGGGCAGGATACGAGGGGCAGCCCTCGACGTGCATGAGTCA
  GAGCCCTTCAGCTTTGCTCAGGGTCCGTTGAAAGATGCCCCGAATCTCATCTGCACTCCT
  CACACTGCCTGGTACAGTGAGCAGGCGTCACTGGAGATGAGGGAGGCAGCTGCCACCGAG
  ATCCGCCGAGCCATCACAGGTCGCATCCCAGAAAGCTTAAGAAATTGTGTGAACAAGGAA
  TTCTTTGTCACATCAGCGCCTTGGTCAGTAATAGACCAGCAAGCAATTCATCCTGAGCTC
  AATGGTGCCACATACAGATATCCGCCAGGCATCGTGGGTGTGGCTCCAGGAGGACTTCCT
  GCAGCCATGGAAGGGATCATCCCTGGAGGCATCCCAGTGACTCACAACCTCCCGACAGTG
  GCACATCCTTCCCAAGCGCCCTCTCCCAACCAGCCCACAAAACACGGGGACAATCGAGAG
  CACCCCAACGAGCAATAG
  
  Clone variation with respect to NM_001329.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001329 unedited
  TAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGAAGTTTTTGTGTGTGTGT
  CCCTGTGTGCGTGTGTCATTTTAAGGTGGCTCGGGAGCGGCCCGGAGGAGCCGGCGGCGG
  CCCGAGGAGCGAGGGACCGAACCGGGAGCCATGCCGCGCTGAGGGGGGGCCGCACAGCCG
  CCGCCACCGCCACCGCCGCCGGGTGGGGTGGGAGGGGGCGGGAGACGCCGCCGCCGCCGC
  CTCCAGGGTGGGCGCCTTTCGCCGTGGACGCCGACCGTCCGGGACGAGGGTTTCATCACC
  TTAAATGGTTTTGAACCAATGAAGGTGTATTCCCTTAAAAAGACGGACAGCCCATCGTGT
  GAACTATAGAGTTTGTGGACAGATTTATATTGGGTTCATAGTGGCGTCATGCACGCAGAC
  TCCTGCAAGTTCCCCTAAGTTCTTAGAGGACTGCTTTGCCTTTTGATCTGAGAGTTGCAA
  AGTTCCATAAAGAATGGCCCTTGTGGATAAGCACAAAGTCAAGAGACAGCGATTGGACAG
  AATTGTGAAGGGNTTATCCGCCCNCAGATCATGAACGGCCCCCTGCACCCCCGCCCCCTG
  GTGGCGCTGNTGGNACGGCCGCGACTGCACTGTGGGNAGATGCCNCATCCTGAAGACCTG
  GCCACTGTGGCCTTCTGTGACGCGCAGTCGACGCAGGAATCCCGNAAAAGGTTCTAACGA
  AGGCCGTGGCGCCATGATGTACACACCATCACCTACNAAGGGAGCTGGNNNAAAATTCAG
  GCCTGAGAAAGGATCGTGCGATAGGCAGTGCTATGACAACGTGACATCAGGCTGNCGCGA
  GCTCGAATGGCGTGGCACATCCGTTGAGCGGGGGAAAAACAGGGACCTACATTGCCATCT
  CACTTACCGAGGACCCGGGTGACAGCTGGGAG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001329 unedited
  GACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGCATCATCAGAGGGTT
  TTACTGAACTTACAACCGACTTGCCCGCTCAGTATGCAGTTCAGATGTGAGAGGCGCTTC
  TCTGTACAGCAGCCTGTACTGTCTTCAATCCTATGCGTGCAGGTGTCTACCACAGGCAAA
  CAGTTTTCTCCCCATTTTGTAGTAATGTGATTTTCCTATTAGCAAAAAGAGGTCACCAGC
  CCCTGTAGACTTAAGGGACTCAAGTCACAGGATGGGGATTTCCTCTTAATATTTTTTATT
  TTGTTGTTTGAACTCTTGATGCAACATTGTAGAGCAGGGTGTTCAGGACCTGCTGTGCCC
  AAGGGACTGATAAAGGAAAAAGCTCTATTTATTCTTTTTGTGATTTGATGCACAGATGAA
  AAACTTAACACACAATAACAGAAGTTGGTCGTTAATAAATCACATCCTAGTCTTTCAGCG
  CTTCCGTAAGCAGACGACATCTTCAGTTTTCTAGCTCTTGTAGTTTCAACACTGCAACAT
  CAATGATGCATATGTCCAGAATCAGTTACAAAGACCATCCGATTCTTTTTCTCTTAGTTC
  ATCTATTTTTCACTGTCTCTTGGTCCCAAGTGTATCTGAGTGATTACCTTCTGGCATTCT
  CTGCTATTGCTCGTTGGGGTGCTCTCGATTGTCCCCGTGTTTTGTGGGCTGGTTGGGAGA
  GGGCGCTNTGGAANGATGTGCCACTGTCGGGAAGGTGTGAGTCACTGGGATGCCTNCAGG
  GATGATCCCTTCCATGGCTGCANGAAGTNCTNCTGGAGCCACACCCACGATGCCNNTGCG
  ATATCTGTATGTGGNCACCATTGAGCTCANGATGAAATNNGCTGCTGGNCTATTACTGAC
  CAAGCGGCTGATGTGACAAAGAATTCCTTTGTCACACATTNCTTAGCTTNCTGGATGCGA
  CCTGTGATGGCTCGCGGATCTCGTGGANNTGCTNNCTCATTCATGACGCTGCTACTGTCC
  AGCNGGTNAGAAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001329
• Insert Size: 2660 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001329.1, NP_001320.1
• RefSeq Size: 2368 bp
• RefSeq ORF: 1338 bp
• Locus ID: 1488
• Cytogenetics: 10q26.13
• Domains: 2-Hacid_DH, 2-Hacid_DH_C
• Protein Families: Stem cell - Pluripotency, Stem cell relevant signaling - Wnt Signaling pathway
• Protein Pathways: Chronic myeloid leukemia, Notch signaling pathway, Pathways in cancer, Wnt signaling pathway
• Gene Summary: 'This gene produces alternative transcripts encoding two distinct proteins. One protein is a transcriptional repressor, while the other isoform is a major component of specialized synapses known as synaptic ribbons. Both proteins contain a NAD+ binding domain similar to NAD+-dependent 2-hydroxyacid dehydrogenases. A portion of the 3' untranslated region was used to map this gene to chromosome 21q21.3; however, it was noted that similar loci elsewhere in the genome are likely. Blast analysis shows that this gene is present on chromosome 10. Several transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Feb 2014]'
  Transcript Variant: This variant (1) contains a distinct 5' UTR and 5' CDS, compared to variant 2. Variants 1, 3, 4, and 5-8 all encode the same isoform (1), which has a distinct N-terminus that is 540 aa shorter than the N-terminus of isoform 2. The protein is thought to bind to the C-terminus of the adenovirus E1A proteins. Studies in mice suggest that this protein is involved in transcriptional repression. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.