Glucokinase (GCK) (BC001890) Human Untagged Clone

CAT#: SC127236

GCK (untagged)-Human glucokinase (hexokinase 4, maturity onset diabetes of the young 2), transcript variant 1 (cDNA clone MGC:1742 IMAGE:3536582), complete cds

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GCK
• Synonyms: ATP:D-hexose 6-phosphotransferase; GK; GLK; glucokinase; glucokinase (hexokinase 4); hexokinase D, pancreatic isozyme; HHF3; HK4; HKIV; HXKP; MODY2; OTTHUMP00000024521; OTTHUMP00000159308
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC127236 sequence for BC001890 edited (data generated by NextGen Sequencing)
  ATGCTGGACGACAGAGCCAGGATGGAGGCCGCCAAGAAGGAGAAGGTAGAGCAGATCCTG
  GCAGAGTTCCAGCTGCAGGAGGAGGACCTGAAGAAGGTGATGAGACGGATGCAGAAGGAG
  ATGGACCGCGGCCTGAGGCTGGAGACCCATGAAGAGGCCAGTGTGAAGATGCTGCCCACC
  TACGTGCGCTCCACCCCAGAAGGCTCAGAAGTCGGGGACTTCCTCTCCCTGGACCTGGGT
  GGCACTAACTTCAGGGTGATGCTGGTGAAGGTGGGAGAAGGTGAGGAGGGGCAGTGGAGC
  GTGAAGACCAAACACCAGATGTACTCCATCCCCGAGGACGCCATGACCGGCACTGCTGAG
  ATGCTCTTCGACTACATCTCTGAGTGCATCTCCGACTTCCTGGACAAGCATCAGATGAAA
  CACAAGAAGCTGCCCCTGGGCTTCACCTTCTCCTTTCCTGTGAGGCACGAAGACATCGAT
  AAGGGCATCCTTCTCAACTGGACCAAGGGCTTCAAGGCCTCAGGAGCAGAAGGGAACAAT
  GTCGTGGGGCTTCTGCGAGACGCTATCAAACGGAGAGGGGACTTTGAAATGGATGTGGTG
  GCAATGGTGAATGACACGGTGGCCACGATGATCTCCTGCTACTACGAAGACCATCAGTGC
  GAGGTCGGCATGATCGTGGGCACGGGCTGCAATGCCTGCTACATGGAGGAGATGCAGAAT
  GTGGAGCTGGTGGAGGGGGACGAGGGCCGCATGTGCGTCAATACCGAGTGGGGCGCCTTC
  GGGGACTCCGGCGAGCTGGACGAGTTCCTGCTGGAGTATGACCGCCTGGTGGACGAGAGC
  TCTGCAAACCCCGGTCAGCAGCTGTATGAGAAGCTCATAGGTGGCAAGTACATGGGCGAG
  CTGGTGCGGCTTGTGCTGCTCAGGCTCGTGGACGAAAACCTGCTCTTCCACGGGGAGGCC
  TCCGAGCAGCTGCGCACACGCGGAGCCTTCGAGACGCGCTTCGTGTCGCAGGTGGAGAGC
  GACACGGGCGACCGCAAGCAGATCTACAACATCCTGAGCACGCTGGGGCTGCGACCCTCG
  ACCACCGACTGCGACATCGTGCGCCGCGCCTGCGAGAGCGTGTCTACGCGCGCTGCGCAC
  ATGTGCTCGGCGGGGCTGGCGGGCGTCATCAACCGCATGCGCGAGAGCCGCAGCGAGGAC
  GTAATGCGCATCACTGTGGGCGTGGATGGCTCCGTGTACAAGCTGCACCCCAGCTTCAAG
  GAGCGGTTCCATGCCAGCGTGCGCAGGCTGACGCCCAGCTGCGAGATCACCTTCATCGAG
  TCGGAGGAGGGCAGTGGCCGGGGCGCGGCCCTGGTCTCGGCGGTGGCCTGTAAGAAGGCC
  TGTATGCTGGGCCAGTGA
  
  Clone variation with respect to BC001890.1
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for BC001890 unedited
  CCCGCCGATANGGTAANTGTNAAACGATTTACTATAGGCGGCCGCGNAATTCGCACGAGG
  GAATCAGGNAAATGCCGAGCGGCGCCTGAGCCCCAGGAAGCAGGCTAGGATGTGAGAGAC
  ACAGTCACCTGCAGCCTAATTACTCAAAAGCTGTCCCCAGGTCACAGAAGGGAGAGGACA
  TTTCCCACTGAATCTGTCTGAAGGACACTAAGCCCCACAGCTCAACACAACCAGGAGAGA
  AAGCGCTGAGGACGCCACCCAAGCGCCCAGCAATGGCCCTGCCTGGAGAACATCCAGGCT
  CAGTGAGGAAGGGTCCAGAAGGGAATGCTTGCCGACTCGTTGGAGAACAATGAAAAGGAG
  GAAACTGTGACTGAACCTCAAACCCCAAACCAGCCCGAGGAGAACCACATTCTCCCAGGG
  ACCCAGGGCGGGCCGTGACCCCTGCGGCGGAGAAGCCTTGGATATTTCCACTTCAGAAGC
  CTACTGGGGAAGGCTGAGGGGTCCCAGCTCCCCACGCTGGCTGCTGTGCAGATGCTGGAC
  GACAGAGCCAGGATGGAGGCCGCCAAGAAGGAGAAGGTAGAGCAGATCCTGGCAGAGTTC
  CAGCTGCAGGAGGAGGACCTGAAGAAGGTGATGAGACGGATGCAGAAGGAGATGGACCGC
  GGCCTGAGGCTGGAGACCCATGAAGAGGCCAGTGTGAAGATGCTGCCCACCTACGTGCGC
  TCCACCCCAGAAGGCTCAGAAGTCGGGGACTTCCTCTCCCTGGACCTGTGTGGCACTAAC
  TTCANGGTGATGCTGGTNGAAGTGTGAAAGGTNGAGAGGGGCAGTGGAGCGTGAAGACAA
  ACACCAGAGTACTCCATCCCCCGAGACGCATGACCGGCACTGCTGAGATGCTCTTCGATC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for BC001890 unedited
  CGTTGCCTTCATTGGACGATTGGCACCTTGCCAGGNCCAGGATAGCACTGGGGNAGTGGG
  TCACAGGGCATTGCCACCCGGGTATCTGTTCAGGAAAACAGCTATGACCGCGGCCGCAAT
  CTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTAAAACTTTTAACATTTTTAATTAAAAA
  ACCCACACAATCTTATGTTGGGCATGGAGAGGGTGGCATGTCATATGATGGGGTTGTCCC
  TTGGGGTGTGCTGAATGCTGCTTGGGGTTTCTTCCTGAGCCAGCGCTATGGGAGCTGAAT
  ATGTAGGGCTGGCCCTTCTCCCCTTTGTGGGCCCCTTGGGGCAGAAGCTTGGGGGCTCTG
  TCTCTTGCACCTGCTGCGAGGGTCCATCCCAGAATCACAAGCCACTCAGTGATGGTATGG
  GAGAGAAAAGGTCTCTGGGGGTATTCAAAGCTTGGGACACCTCCTCCATGGAGCCCCCTC
  CCATTGCTCCCTGGGCCTCTTGAGCCTCAGGCAAGAAAATGGCCAGGACTGGGTGATGAG
  CAGCCCCTGGCTGCCCTTTTGGCCCAAGTCTAGGTCTGGTCAAGCTGTTGGAGCTGCCTC
  CCCACACAGGATGAGTTCCCAGGGAAGTGAGGCCACAGCAGGATCCAGAGGGCACCCCTT
  CCCAGAGCCTGGGCCCAGGCGAGGCCAACAGCTCTGACAGTGTGGAAATGCATCAAAGTC
  CTGAGTGAGCAACTCCCTTCTGGGAAATTGATTCCAGCGAGAAAGGTGGGGGGGCTTCCA
  GAGTCTCTGTTCCTGCTCCTGCCGCACCCCCGTTAGGGGCCTGGGGCTGTCCCACCGAAA
  ACTGATGGAAGAGCCCCTCTGTCCACGTTTTTTGGGAAAANNNNCTTTGGTCCTGAAAGG
  GCAAGCCTCCTGCCCCGCCAT
  
• Restriction Sites: Please inquire
• ACCN: BC001890
• Insert Size: 3000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC001890.1, AAH01890.1
• RefSeq Size: 2748 bp
• Locus ID: 2645
• Cytogenetics: 7p13
• Protein Families: Druggable Genome
• Protein Pathways: Amino sugar and nucleotide sugar metabolism, Galactose metabolism, Glycolysis / Gluconeogenesis, Insulin signaling pathway, Maturity onset diabetes of the young, Metabolic pathways, Starch and sucrose metabolism, Type II diabetes mellitus
• Gene Summary: 'This gene encodes a member of the hexokinase family of proteins. Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. In contrast to other forms of hexokinase, this enzyme is not inhibited by its product glucose-6-phosphate but remains active while glucose is abundant. The use of multiple promoters and alternative splicing of this gene result in distinct protein isoforms that exhibit tissue-specific expression in the pancreas and liver. In the pancreas, this enzyme plays a role in glucose-stimulated insulin secretion, while in the liver, this enzyme is important in glucose uptake and conversion to glycogen. Mutations in this gene that alter enzyme activity have been associated with multiple types of diabetes and hyperinsulinemic hypoglycemia. [provided by RefSeq, Aug 2017]'