GOT2 (NM_002080) Human Untagged Clone

CAT#: SC127269

GOT2 (untagged)-Human glutamic-oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase 2) (GOT2), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GOT2
• Synonyms: KAT4; KATIV; KYAT4; mitAAT
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC127269 sequence for NM_002080 edited (data generated by NextGen Sequencing)
  ATGGCCCTGCTGCACTCCGGCCGCGTCCTCCCCGGGATCGCCGCCGCCTTCCACCCGGGC
  CTCGCCGCCGCGGCCTCTGCCAGAGCCAGCTCCTGGTGGACCCATGTGGAAATGGGACCT
  CCAGATCCCATTCTGGGAGTCACTGAAGCCTTTAAGAGGGACACCAATAGCAAAAAGATG
  AATCTGGGAGTTGGTGCCTACCGGGATGATAACGGAAAGCCTTACGTGCTGCCTAGCGTC
  CGCAAGGCAGAGGCCCAGATTGCCGCAAAAAATTTGGACAAGGAATACCTGCCCATTGGG
  GGACTGGCTGAATTTTGCAAGGCATCTGCAGAACTAGCCCTGGGTGAGAACAGCGAAGTC
  TTGAAGAGTGGCCGGTTTGTCACTGTGCAGACCATTTCTGGAACTGGAGCCTTAAGGATC
  GGAGCCAGTTTTCTGCAAAGATTTTTTAAGTTCAGCCGAGATGTCTTTCTGCCCAAACCA
  ACCTGGGGAAACCACACACCCATCTTCAGGGATGCTGGCATGCAGCTACAAGGTTATCGG
  TATTATGACCCCAAGACTTGCGGTTTTGACTTCACAGGCGCTGTGGAGGATATTTCAAAA
  ATACCAGAGCAGAGTGTTCTTCTTCTGCATGCCTGCGCCCACAATCCCACGGGAGTGGAC
  CCGCGTCCGGAACAGTGGAAGGAAATAGCAACAGTGGTGAAGAAAAGGAATCTCTTTGCG
  TTCTTTGACATGGCCTACCAAGGCTTTGCCAGTGGTGATGGTGATAAGGATGCCTGGGCT
  GTGCGCCACTTCATCGAACAGGGCATTAATGTTTGTCTCTGCCAATCATATGCCAAGAAC
  ATGGGCTTATATGGTGAGCGTGTAGGAGCCTTCACTATGGTCTGCAAAGATGCGGATGAA
  GCCAAAAGGGTAGAGTCACAGTTGAAGATCTTGATCCGTCCCATGTATTCCAACCCTCCC
  CTCAATGGGGCCCGGATTGCTGCTGCCATTCTGAACACCCCAGATTTGCGAAAACAATGG
  CTGCAAGAAGTGAAAGGCATGGCTGACCGCATCATTGGCATGCGGACTCAACTGGTCTCC
  AACCTCAAGAAGGAGGGTTCCACCCACAATTGGCAACACATCACCGACCAAATTGGCATG
  TTCTGTTTCACAGGGCTAAAGCCTGAACAGGTGGAGCGGCTGATCAAGGAGTTCTCCATC
  TACATGACAAAAGATGGCCGCATCTCTGTGGCAGGGGTCACCTCCAGCAACGTGGGCTAC
  CTTGCCCATGCCATTCACCAGGTCACCAAGTAA
  
  Clone variation with respect to NM_002080.2
  213 t=>c;228 t=>g;816 c=>t;1037 t=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002080 unedited
  GAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGTTCCCTGTGTGTGTGTC
  CGCTCGCCCTCTGCTCCGTCCTGCGGCTGCCCACTGCCCTCCTACGGTCCACCATGGCCC
  TGCTGCACTCCGGCCGCGTCCTCCCCGGGATCGCCGCCGCCTTCCACCCGGGCCTCGCCG
  CCGCGGCCTCTGCCAGAGCCAGCTCCTGGTGGACCCATGTGGAAATGGGACCTCCAGATC
  CCATTCTGGGAGTCACTGAAGCCTTTAAGAGGGACACCAATAGCAAAAAGATGAATCTGG
  GAGTTGGTGCCTACCGGGATGATAACGGAAAGCCTTACGTGCTGCCTAGCGTCCGCAAGG
  CAGAGGCCCAGATTGCCGCAAAAAATTTGGACAAGGAATACCTGCCCATTGGGGGACTGG
  CTGAATTTTGCAAGGCATCTGCAGAACTAGCCCTGGGTGAGAACAGCGAAGTCTTGAAGA
  GTGGCCGGTTTGTCACTGTGCAGACCATTTCTGGAACTGGAGCCTTAAGGATCGGAGCCA
  GTTTTCTGCAAAGATTTTTTAAGTTCAGCCGAGATGTCTTTCTGCCCAAACCAACCTGGG
  GAAACCACACACCCATCTTCAGGGATGCTGGCATGCAGCTACAAGGTTATCGGTATTATG
  ACCCCAAGACTTGCGGTTTTGACTTCACAGGCGCTGTGGAAGATATTTCAAAATACCAGA
  GCAGAGTGTTCCTCTTCTGCATGCCTGCGCCCACAATCCCAGGGAGTGGACCCGCGTCCG
  GAAAGTGGGAGGAAATACCACCAGCGTGAAGAAAAGGAATCTCTTTGCGTNCTTTGACTT
  GCCCTACACAGCCTTGCCAGTGTGGATGGTGATAAGATGCCTGGGCTGGGCCCCACTCAT
  CG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002080 unedited
  GGCCGCAATCTAGNATCGAGTTTTTTTTTTTTTTTTTTGGGTCTACAGTAATTTTATTGT
  AACAGAGAAACCAGGCCGCAATAAGTCTACATGGTTAGAGCAGATGGTGGTTCTTTCCAG
  TGGGTGAAGCCTGAACCCAGCTAGCAGCACGCAAAGCCTAGAATATCCTCTTTCCTGGTA
  CCACCCTGCGCCGCTGGACTCACAGTGTGAGATGAGAGGCTGCACACCACATTCAACAAA
  GGGAGGAGGTCCAACCGGGCAGAGACAACATCCCAACTGGAGAAACTTGCACTATCATTC
  AAGAGGATGCCGAGATAAAGTACAGTTTCCTACTCTCCACTATTCTTTGCTGAAAAGGGT
  GAGGGTACGGCATCAGCAGAAAATTATGTTCTTCATTCTTAAATAAATCTCGATAGGATG
  GAGTTGATGGTCAATCAAGCAGTAGAATGGGATGAAAATCTGCAATAGTTAAAAAATCTT
  TGCGTGTAAGGAAAAGAACGGCAATGACAAGGGAGAAAAGAGAGCCTTCTCTTTTTCTTA
  AATAAGACAGGAAACTGCATTATTTCCCTTGCTGGAGTGGCAGCAGCCTCATTGGTTTTC
  ATAAAACATCATTTCTGCAGTGCGGCCTGTTGATAAGGGTGTTCCAAGATGGGAGGACAG
  ACACGAGGCCGACTACTCATGACAGAACTAGGCCTTCTCTCAAAGCCTCTAACGTGTGCT
  GTTTCTCACGAGGAACCTGACACTTCAGNTAAAGCTTCTGAATATTGCAATGGCTATTGA
  CAGGTTTTTGTGCGATGACTTCTGCCATGTAACTCTTGAGAAAGTTGAGAAAAAGGACGC
  GAGAGTTCGGTTATATTCCAACGCACCCTGATGCCTTGCCTGGGACTACAGGTCTTTTGA
  AACATCAATGTGAAGGTAACCTGGGCTGAAGAAGATCATACCCCCATCACCTTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002080
• Insert Size: 2410 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002080.2, NP_002071.2
• RefSeq Size: 2473 bp
• RefSeq ORF: 1293 bp
• Locus ID: 2806
• Cytogenetics: 16q21
• Domains: aminotran_1_2
• Protein Families: Stem cell - Pluripotency
• Protein Pathways: Alanine, aspartate and glutamate metabolism, Arginine and proline metabolism, Cysteine and methionine metabolism, Metabolic pathways, Phenylalanine, tyrosine and tryptophan biosynthesis, Phenylalanine metabolism, Tyrosine metabolism
• Gene Summary: 'Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and inner-membrane mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2013]'
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).