HIF-1 alpha (HIF1A) (NM_181054) Human Untagged Clone

CAT#: SC127304

HIF1A (untagged)-Human hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) (HIF1A), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HIF1A
• Synonyms: bHLHe78; HIF-1-alpha; HIF-1A; HIF-1alpha; HIF1; HIF1-ALPHA; MOP1; PASD8
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC127304 sequence for NM_181054 edited (data generated by NextGen Sequencing)
  ATGGAGGGCGCCGGCGGCGCGAACGACAAGAAAAAGATAAGTTCTGAACGTCGAAAAGAA
  AAGTCTCGAGATGCAGCCAGATCTCGGCGAAGTAAAGAATCTGAAGTTTTTTATGAGCTT
  GCTCATCAGTTGCCACTTCCACATAATGTGAGTTCGCATCTTGATAAGGCCTCTGTGATG
  AGGCTTACCATCAGCTATTTGCGTGTGAGGAAACTTCTGGATGCTGGTGATTTGGATATT
  GAAGATGACATGAAAGCACAGATGAATTGCTTTTATTTGAAAGCCTTGGATGGTTTTGTT
  ATGGTTCTCACAGATGATGGTGACATGATTTACATTTCTGATAATGTGAACAAATACATG
  GGATTAACTCAGTTTGAACTAACTGGACACAGTGTGTTTGATTTTACTCATCCATGTGAC
  CATGAGGAAATGAGAGAAATGCTTACACACAGAAATGGCCTTGTGAAAAAGGGTAAAGAA
  CAAAACACACAGCGAAGCTTTTTTCTCAGAATGAAGTGTACCCTAACTAGCCGAGGAAGA
  ACTATGAACATAAAGTCTGCAACATGGAAGGTATTGCACTGCACAGGCCACATTCACGTA
  TATGATACCAACAGTAACCAACCTCAGTGTGGGTATAAGAAACCACCTATGACCTGCTTG
  GTGCTGATTTGTGAACCCATTCCTCACCCATCAAATATTGAAATTCCTTTAGATAGCAAG
  ACTTTCCTCAGTCGACACAGCCTGGATATGAAATTTTCTTATTGTGATGAAAGAATTACC
  GAATTGATGGGATATGAGCCAGAAGAACTTTTAGGCCGCTCAATTTATGAATATTATCAT
  GCTTTGGACTCTGATCATCTGACCAAAACTCATCATGATATGTTTACTAAAGGACAAGTC
  ACCACAGGACAGTACAGGATGCTTGCCAAAAGAGGTGGATATGTCTGGGTTGAAACTCAA
  GCAACTGTCATATATAACACCAAGAATTCTCAACCACAGTGCATTGTATGTGTGAATTAC
  GTTGTGAGTGGTATTATTCAGCACGACTTGATTTTCTCCCTTCAACAAACAGAATGTGTC
  CTTAAACCGGTTGAATCTTCAGATATGAAAATGACTCAGCTATTCACCAAAGTTGAATCA
  GAAGATACAAGTAGCCTCTTTGACAAACTTAAGAAGGAACCTGATGCTTTAACTTTGCTG
  GCCCCAGCCGCTGGAGACACAATCATATCTTTAGATTTTGGCAGCAACGACACAGAAACT
  GATGACCAGCAACTTGAGGAAGTACCATTATATAATGATGTAATGCTCCCCTCACCCAAC
  GAAAAATTACAGAATATAAATTTGGCAATGTCTCCATTACCCACCGCTGAAACGCCAAAG
  CCACTTCGAAGTAGTGCTGACCCTGCACTCAATCAAGAAGTTGCATTAAAATTAGAACCA
  AATCCAGAGTCACTGGAACTTTCTTTTACCATGCCCCAGATTCAGGATCAGACACCTAGT
  CCTTCCGATGGAAGCACTAGACAAAGTTCACCTGAGCCTAATAGTCCCAGTGAATATTGT
  TTTTATGTGGATAGTGATATGGTCAATGAATTCAAGTTGGAATTGGTAGAAAAACTTTTT
  GCTGAAGACACAGAAGCAAAGAACCCATTTTCTACTCAGGACACAGATTTAGACTTGGAG
  ATGTTAGCTCCCTATATCCCAATGGATGATGACTTCCAGTTACGTTCCTTCGATCAGTTG
  TCACCATTAGAAAGCAGTTCCGCAAGCCCTGAAAGCGCAAGTCCTCAAAGCACAGTTACA
  GTATTCCAGCAGACTCAAATACAAGAACCTACTGCTAATGCCACCACTACCACTGCCACC
  ACTGATGAATTAAAAACAGTGACAAAAGACCGTATGGAAGACATTAAAATATTGATTGCA
  TCTCCATCTCCTACCCACATACATAAAGAAACTACTAGTGCCACATCATCACCATATAGA
  GATACTCAAAGTCGGACAGCCTCACCAAACAGAGCAGGAAAAGGAGTCATAGAACAGACA
  GAAAAATCTCATCCAAGAAGCCCTAACGTGTTATCTGTCGCTTTGAGTCAAAGAACTACA
  GTTCCTGAGGAAGAACTAAATCCAAAGATACTAGCTTTGCAGAATGCTCAGAGAAAGCGA
  AAAATGGAACATGATGGTTCACTTTTTCAAGCAGTAGGAATTATTTAG
  
  Clone variation with respect to NM_181054.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_181054 unedited
  TGTATACGACCTCACTATAGGGCGGCCGCGAATTCGCACGAGGCACCTCTGGACTTGCCT
  TTCCTTCTCTTCTCCGCGTGTGGAGGGAGCCAGCGCTTAGGCCGGAGCGAGCCTGGGGGC
  CGCCCGCCGTGAAGACATCGCGGGGACCGATTCACCATGGAGGGCGCCGGCGGCGCGAAC
  GACAAGAAAAAGATAAGTTCTGAACGTCGAAAAGAAAAGTCTCGAGATGCAGCCAGATCT
  CGGCGAAGTAAAGAATCTGAAGTTTTTTATGAGCTTGCTCATCAGTTGCCACTTCCACAT
  AATGTGAGTTCGCATCTTGATAAGGCCTCTGTGATGAGGCTTACCATCAGCTATTTGCGT
  GTGAGGAAACTTCTGGATGCTGGTGATTTGGATATTGAAGATGACATGAAAGCACAGATG
  AATTGCTTTTATTTGAAAGCCTTGGATGGTTTTGTTATGGTTCTCACAGATGATGGTGAC
  ATGATTTACATTTCTGATAATGTGAACAAATACATGGGATTAACTCAGTTTGAACTAACT
  GGACACAGTGTGTTTGATTTTACTCATCCATGTGACCATGAGGAAATGAGAGAAATGCTT
  ACCACAGAAATGGCCTTGTGAAAAAGGGTAAAGAACAAAACACACAGCGAAGCTTTTTTC
  TCAAATGAAGTGTACCCTAACTAGCCGAGGAAGAACTATGAACATAAAGTCTGCACATGG
  GAAGGTATTGCACTGCACAGGCCCATTCACGTATATGATACCACAGTAACCAACCTCAGT
  GTGGGGATAGAAACCACTATGACCTGGCTGGTGCTGATTTGGGACCATTTCTCACCTCAA
  TATGGAAANTCTTAATAGCAGACTTTCTCATCGAACACCTGAATGAAATTNCTTATGGAT
  GAAGATACCGATGATGGNAATAGCCAAGAATTTTAGCCGCCATTTGAAATATAGGCTGAA
  CA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_181054 unedited
  AATCTAGAGTCGAGTTTTTNTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCCAAAAAATGT
  TTATTTGATGTAACAAAACAATACAGTTAGTGTTAGATCCAACCACAAAGAGCAAAAGGA
  ATGAAAAATTTGTCCATTGTCCATAGAAAAAACAAGATTTTTACTGGGACAACTATATAT
  TCCTAAAATAATGCTTCTAAAATTACTCAATTATTGAAATCTACATGAAAAAAAGGATGT
  TAATAGCGACAAAGTGCATAAAATCAAACATTGTATTTTGAGCAAATTAACATACTAGGC
  AATTTTGCTAAAAATGCATGATTTTTTTTTTCTTGTTAACAGTCTGCTCAAAATATCTTT
  ATACCAACAGGGTAGGCAAAACATTTAGGTTTAATATCAGTTACACAATATTAGCATAAA
  CTTCCACAACTACATAGGGTATTGTTTTCTTTTGAGCTGGCAAAGGGACTATAAAAACAT
  CAGATGATTTCTCTGAATTGAAAATTTTATCCAAATAAATGCCACATACCTTCTAGATAT
  ATGCATATCTTTCTATATTATGTAAATGGCTTTACCCATTTAAATAATAAACCATACAGC
  ATTTAAGAATCATTATTATATGATTAACAATGTCATGTTCCAGGTTTAACAATTTCATAG
  GCCAAAAAAAATTTCTTCTTAAAAACTAGTTTTATAAACGCAGAATTATTCCATGAGTAC
  TGCTGGTATTTTTTAAGAAATATATTGTGCAATTGTGGCTACCACGTACTGCTGGCAAAG
  CATATTATTTATGTAAAATGTGAAAAAAAGGTGTAAAATTTTTCACTGCTATGATATGAG
  GAAGGTACCGGCTTCTACAAAATTTATGGGCATCTCTAAAATATTCGAAAAGGATAACTC
  CTAGCCAATTATANTAAAAGGGCATTTTAAATGAGCTCTGATGCATGGTTATACAACATG
  AAATGACTGTTGGGATCACTTAGGACTCTAAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_181054
• Insert Size: 4000 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_181054.1, NP_851397.1
• RefSeq Size: 3812 bp
• RefSeq ORF: 2208 bp
• Locus ID: 3091
• Cytogenetics: 14q23.2
• Protein Families: Transcription Factors
• Protein Pathways: mTOR signaling pathway, Pathways in cancer, Renal cell carcinoma
• Gene Summary: 'This gene encodes the alpha subunit of transcription factor hypoxia-inducible factor-1 (HIF-1), which is a heterodimer composed of an alpha and a beta subunit. HIF-1 functions as a master regulator of cellular and systemic homeostatic response to hypoxia by activating transcription of many genes, including those involved in energy metabolism, angiogenesis, apoptosis, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF-1 thus plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jul 2011]'
  Transcript Variant: This variant (2) lacks the penultimate coding exon compared to variant 1. This results in a frame-shift, and a shorter isoform (2) with a distinct C-terminus compared to isoform 1.