HIF-1 alpha (HIF1A) (NM_181054) Human Untagged Clone

CAT#: SC127304

HIF1A (untagged)-Human hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) (HIF1A), transcript variant 2


  "NM_181054" in other vectors (6)

Reconstitution Protocol

USD 1,480.00

In Stock*

Size
    • 10 ug

Product Images

Other products for "HIF1A"

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol HIF1A
Synonyms bHLHe78; HIF-1-alpha; HIF-1A; HIF-1alpha; HIF1; HIF1-ALPHA; MOP1; PASD8
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC127304 sequence for NM_181054 edited (data generated by NextGen Sequencing)
ATGGAGGGCGCCGGCGGCGCGAACGACAAGAAAAAGATAAGTTCTGAACGTCGAAAAGAA
AAGTCTCGAGATGCAGCCAGATCTCGGCGAAGTAAAGAATCTGAAGTTTTTTATGAGCTT
GCTCATCAGTTGCCACTTCCACATAATGTGAGTTCGCATCTTGATAAGGCCTCTGTGATG
AGGCTTACCATCAGCTATTTGCGTGTGAGGAAACTTCTGGATGCTGGTGATTTGGATATT
GAAGATGACATGAAAGCACAGATGAATTGCTTTTATTTGAAAGCCTTGGATGGTTTTGTT
ATGGTTCTCACAGATGATGGTGACATGATTTACATTTCTGATAATGTGAACAAATACATG
GGATTAACTCAGTTTGAACTAACTGGACACAGTGTGTTTGATTTTACTCATCCATGTGAC
CATGAGGAAATGAGAGAAATGCTTACACACAGAAATGGCCTTGTGAAAAAGGGTAAAGAA
CAAAACACACAGCGAAGCTTTTTTCTCAGAATGAAGTGTACCCTAACTAGCCGAGGAAGA
ACTATGAACATAAAGTCTGCAACATGGAAGGTATTGCACTGCACAGGCCACATTCACGTA
TATGATACCAACAGTAACCAACCTCAGTGTGGGTATAAGAAACCACCTATGACCTGCTTG
GTGCTGATTTGTGAACCCATTCCTCACCCATCAAATATTGAAATTCCTTTAGATAGCAAG
ACTTTCCTCAGTCGACACAGCCTGGATATGAAATTTTCTTATTGTGATGAAAGAATTACC
GAATTGATGGGATATGAGCCAGAAGAACTTTTAGGCCGCTCAATTTATGAATATTATCAT
GCTTTGGACTCTGATCATCTGACCAAAACTCATCATGATATGTTTACTAAAGGACAAGTC
ACCACAGGACAGTACAGGATGCTTGCCAAAAGAGGTGGATATGTCTGGGTTGAAACTCAA
GCAACTGTCATATATAACACCAAGAATTCTCAACCACAGTGCATTGTATGTGTGAATTAC
GTTGTGAGTGGTATTATTCAGCACGACTTGATTTTCTCCCTTCAACAAACAGAATGTGTC
CTTAAACCGGTTGAATCTTCAGATATGAAAATGACTCAGCTATTCACCAAAGTTGAATCA
GAAGATACAAGTAGCCTCTTTGACAAACTTAAGAAGGAACCTGATGCTTTAACTTTGCTG
GCCCCAGCCGCTGGAGACACAATCATATCTTTAGATTTTGGCAGCAACGACACAGAAACT
GATGACCAGCAACTTGAGGAAGTACCATTATATAATGATGTAATGCTCCCCTCACCCAAC
GAAAAATTACAGAATATAAATTTGGCAATGTCTCCATTACCCACCGCTGAAACGCCAAAG
CCACTTCGAAGTAGTGCTGACCCTGCACTCAATCAAGAAGTTGCATTAAAATTAGAACCA
AATCCAGAGTCACTGGAACTTTCTTTTACCATGCCCCAGATTCAGGATCAGACACCTAGT
CCTTCCGATGGAAGCACTAGACAAAGTTCACCTGAGCCTAATAGTCCCAGTGAATATTGT
TTTTATGTGGATAGTGATATGGTCAATGAATTCAAGTTGGAATTGGTAGAAAAACTTTTT
GCTGAAGACACAGAAGCAAAGAACCCATTTTCTACTCAGGACACAGATTTAGACTTGGAG
ATGTTAGCTCCCTATATCCCAATGGATGATGACTTCCAGTTACGTTCCTTCGATCAGTTG
TCACCATTAGAAAGCAGTTCCGCAAGCCCTGAAAGCGCAAGTCCTCAAAGCACAGTTACA
GTATTCCAGCAGACTCAAATACAAGAACCTACTGCTAATGCCACCACTACCACTGCCACC
ACTGATGAATTAAAAACAGTGACAAAAGACCGTATGGAAGACATTAAAATATTGATTGCA
TCTCCATCTCCTACCCACATACATAAAGAAACTACTAGTGCCACATCATCACCATATAGA
GATACTCAAAGTCGGACAGCCTCACCAAACAGAGCAGGAAAAGGAGTCATAGAACAGACA
GAAAAATCTCATCCAAGAAGCCCTAACGTGTTATCTGTCGCTTTGAGTCAAAGAACTACA
GTTCCTGAGGAAGAACTAAATCCAAAGATACTAGCTTTGCAGAATGCTCAGAGAAAGCGA
AAAATGGAACATGATGGTTCACTTTTTCAAGCAGTAGGAATTATTTAG

Clone variation with respect to NM_181054.2
>OriGene 5' read for NM_181054 unedited
TGTATACGACCTCACTATAGGGCGGCCGCGAATTCGCACGAGGCACCTCTGGACTTGCCT
TTCCTTCTCTTCTCCGCGTGTGGAGGGAGCCAGCGCTTAGGCCGGAGCGAGCCTGGGGGC
CGCCCGCCGTGAAGACATCGCGGGGACCGATTCACCATGGAGGGCGCCGGCGGCGCGAAC
GACAAGAAAAAGATAAGTTCTGAACGTCGAAAAGAAAAGTCTCGAGATGCAGCCAGATCT
CGGCGAAGTAAAGAATCTGAAGTTTTTTATGAGCTTGCTCATCAGTTGCCACTTCCACAT
AATGTGAGTTCGCATCTTGATAAGGCCTCTGTGATGAGGCTTACCATCAGCTATTTGCGT
GTGAGGAAACTTCTGGATGCTGGTGATTTGGATATTGAAGATGACATGAAAGCACAGATG
AATTGCTTTTATTTGAAAGCCTTGGATGGTTTTGTTATGGTTCTCACAGATGATGGTGAC
ATGATTTACATTTCTGATAATGTGAACAAATACATGGGATTAACTCAGTTTGAACTAACT
GGACACAGTGTGTTTGATTTTACTCATCCATGTGACCATGAGGAAATGAGAGAAATGCTT
ACCACAGAAATGGCCTTGTGAAAAAGGGTAAAGAACAAAACACACAGCGAAGCTTTTTTC
TCAAATGAAGTGTACCCTAACTAGCCGAGGAAGAACTATGAACATAAAGTCTGCACATGG
GAAGGTATTGCACTGCACAGGCCCATTCACGTATATGATACCACAGTAACCAACCTCAGT
GTGGGGATAGAAACCACTATGACCTGGCTGGTGCTGATTTGGGACCATTTCTCACCTCAA
TATGGAAANTCTTAATAGCAGACTTTCTCATCGAACACCTGAATGAAATTNCTTATGGAT
GAAGATACCGATGATGGNAATAGCCAAGAATTTTAGCCGCCATTTGAAATATAGGCTGAA
CA
>OriGene 3' read for NM_181054 unedited
AATCTAGAGTCGAGTTTTTNTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCCAAAAAATGT
TTATTTGATGTAACAAAACAATACAGTTAGTGTTAGATCCAACCACAAAGAGCAAAAGGA
ATGAAAAATTTGTCCATTGTCCATAGAAAAAACAAGATTTTTACTGGGACAACTATATAT
TCCTAAAATAATGCTTCTAAAATTACTCAATTATTGAAATCTACATGAAAAAAAGGATGT
TAATAGCGACAAAGTGCATAAAATCAAACATTGTATTTTGAGCAAATTAACATACTAGGC
AATTTTGCTAAAAATGCATGATTTTTTTTTTCTTGTTAACAGTCTGCTCAAAATATCTTT
ATACCAACAGGGTAGGCAAAACATTTAGGTTTAATATCAGTTACACAATATTAGCATAAA
CTTCCACAACTACATAGGGTATTGTTTTCTTTTGAGCTGGCAAAGGGACTATAAAAACAT
CAGATGATTTCTCTGAATTGAAAATTTTATCCAAATAAATGCCACATACCTTCTAGATAT
ATGCATATCTTTCTATATTATGTAAATGGCTTTACCCATTTAAATAATAAACCATACAGC
ATTTAAGAATCATTATTATATGATTAACAATGTCATGTTCCAGGTTTAACAATTTCATAG
GCCAAAAAAAATTTCTTCTTAAAAACTAGTTTTATAAACGCAGAATTATTCCATGAGTAC
TGCTGGTATTTTTTAAGAAATATATTGTGCAATTGTGGCTACCACGTACTGCTGGCAAAG
CATATTATTTATGTAAAATGTGAAAAAAAGGTGTAAAATTTTTCACTGCTATGATATGAG
GAAGGTACCGGCTTCTACAAAATTTATGGGCATCTCTAAAATATTCGAAAAGGATAACTC
CTAGCCAATTATANTAAAAGGGCATTTTAAATGAGCTCTGATGCATGGTTATACAACATG
AAATGACTGTTGGGATCACTTAGGACTCTAAA
Restriction Sites NotI-NotI     
ACCN NM_181054
Insert Size 4000 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_181054.1, NP_851397.1
RefSeq Size 3812 bp
RefSeq ORF 2208 bp
Locus ID 3091
Cytogenetics 14q23.2
Protein Families Transcription Factors
Protein Pathways mTOR signaling pathway, Pathways in cancer, Renal cell carcinoma
Gene Summary 'This gene encodes the alpha subunit of transcription factor hypoxia-inducible factor-1 (HIF-1), which is a heterodimer composed of an alpha and a beta subunit. HIF-1 functions as a master regulator of cellular and systemic homeostatic response to hypoxia by activating transcription of many genes, including those involved in energy metabolism, angiogenesis, apoptosis, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF-1 thus plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jul 2011]'
Transcript Variant: This variant (2) lacks the penultimate coding exon compared to variant 1. This results in a frame-shift, and a shorter isoform (2) with a distinct C-terminus compared to isoform 1.

Other Versions

{0} Product Review(s)

0 Product Review(s) Submit review

Be the first one to submit a review

Product Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.