Methionyl tRNA synthetase (MARS) (AK092986) Human Untagged Clone

CAT#: SC127390

(untagged)-Human cDNA FLJ35667 fis, clone SPLEN2018098, highly similar to METHIONYL-TRNA SYNTHETASE (EC 6.1.1.10)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MARS
• Synonyms: CMT2U|ILFS2|ILLD|METRS|MRS|MTRNS|SPG70
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for AK092986, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGACCTCACTGACTCACATTGACCACAGCTTGAGTCGTCAGAACTGTCCTTTCCTGGCTGGGGAGACAG
  AATCTCTAGCCGACATTGTTTTGTGGGGAGCCCTATACCCATTACTGCAAGATCCCGCCTACCTCCCTGA
  GGAGCTGAGTGCCCTGCACAGCTGGTTCCAGACACTGAGTACCCAGGAACCATGTCAGCGAGCTGCAGAG
  ACTGTACTGAAACAGCAAGGTGTCCTGGCTCTCCGGCCTCACCTCCAAAAGCAGCCCCAGCCCAGCCCCG
  CTGAGGGAAGGGCTGTCACCAATGAGCCTGAGGAGGAGGAGCTGGCTACCCTATCTGAGGAGGAGATTGC
  TATCTGCTGTACATTCGGCCTGAGGGCCAGGACAGTGCTTTCTCCTGGACGGACCTGCTGCTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for AK092986 unedited
  AGTATTTTGTAATACGACTCACTTATAGGGCGGCCGCGAATTCGCACGAGGGCGAAATGA
  GACTGTTCGTGAGTGATGGCGTCCCGGGTTGCTTGCCGGTGCTGGCCGCCGCCGGGAGAG
  CCCGGGGCAGAGCAGAGGTGCTCATCAGCACTGTAGGCCCGGAAGATTGTGTGGTCCCGT
  TCCTGACCCGGCCTAAGGTCCCTGTCTTGCAGCTGGATAGCGGCAACTACCTCTTCTCCA
  CTAGTGCAATCTGCCGATATTTTTTTTTGTTATCTGGCTGGGAGCAAGATGACCTCACTA
  ACCAGTGGCTGGAATGGGAAGCGACAGAGCTGCAGCCAGCTTTGTCTGCTGCCCTGTACT
  ATTTAGTGGTCCAAGGCAAGAAGGGGGAAGATGTTCTTGGTTCAGTGCGGAGAGCCCTGA
  CTCACATTGACCACAGCTTGAGTCGTCAGAACTGTCCTTTCCTGGCTGGGGAGACAGAAT
  CTCTAGCCGACATTGTTTTGTGGGGAGCCCTATACCCATTACTGCAAGATCCCGCCTACC
  TCCCTGAGGAGCTGAGTGCCCTGCACAGCTGGTTCCAGACACTGAGTACCCAGGAACCAT
  GTCAGCGAGCTGCAGAGACTGTACTGAAACAGCAAGGTGTCCTGGCTCTCCGGCCTTACC
  TCCAAAAGCAGCCCCAGCCCAGCCCCGCTGAGGGAAGGGCTGTCACCAATGAGCCTGAGG
  AGGAAGAGCTGGCTACCCTATCTGAGGAGGAGATTGCTATGGCCTGTACTGCTTGGGAGA
  AGGCCTANAAGTTTGCCCCGCTGCGGCCCCCACAAATCAGTGTTGCCTGTGGCTGGAGAA
  AGGAAGTGCTCATCACCAGGGCCCTCCCTA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for AK092986 unedited
  CGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGAGATTGTACATTTATTTATTACTG
  TCCCTATCTATTAAAGTGACTTTCTATGAGCCAAGGTCTTTTACTTTTTCTTCTTGCCTT
  TAAGGGCTTCAAGGGGTTTCCCCTCAGCTACAGCCAACTGTTTCTTTAGATCCAAGAGTT
  TCGCCACCTCCGCAGCAACCTCGTTCTTGTCTGCCTTTTGTGCTTTCAGTTCTCGGACAA
  TGTTTCCTTGTTTTGTCACTTCATCCATCAGCGCTTGTATCTGCTGTGGCTTGGCTGTTG
  TAACAGTCTCTACAACTGCTGGCTTCGGGGACGTTTTTGCCTGGCCCCCTCCAAAGCGCT
  GCCTTAAACTTTCAATCTGGTCATTTTCCAATTTTTGGAACAAGGGACTGACTGTGCCAA
  TCTGGTGTCCTGCTGGTAAAGTACACAGGAAGTTTGTCAGCAGGATACTGCACGCTGGAA
  GTGGGAGCTGCAGCTGGGCCTGGATTGTGGCACTAACCGTGGGCATGTAAGGCTGAAACA
  TGACAGAGAGCCAGGCCACCTTTTTCTCTGGCAAGCCATTCACTTGTCCTGCCCGTTGCC
  TTGTAGCCCCACTTGCCTTATTCGCTTCAAGGCCTATTCACCCGAATAATGGGTGCCTGG
  CCAGATTTGTAAGATACCGCCCAGCATCCCGGTTCCACCCTTTTAATTCTGGGGATTAGT
  TGGACCCCTGGTGCATGGCCAGCGGCCTTTTCTTAAGGGCGACTCCTCTCAGCCCTACCC
  CCAAAACTTTTACAACTTCCACTTCCTTACAATTTCCCACTTTATACCACTAAATTTTCT
  TTTACTGCGCGCCCCAATAATCCGTCCTGACCCTTGCCAACTCCATTATTAACCTCCATG
  TTTTGCTCCCGTCCTGACTATCCCAACTCCTCTCTTCCCTAAAATTCCTCCACTTTAN
  
• Restriction Sites: NotI-NotI
• ACCN: AK092986
• Insert Size: 2870 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: AK092986.1, BAC04011.1
• RefSeq Size: 2695 bp
• RefSeq ORF: 414 bp
• Locus ID: 4141
• Cytogenetics: 12q13.3
• Protein Families: Druggable Genome
• Protein Pathways: Aminoacyl-tRNA biosynthesis, Selenoamino acid metabolism
• Gene Summary: 'This gene encodes a member of the class I family of aminoacyl-tRNA synthetases. These enzymes play a critical role in protein biosynthesis by charging tRNAs with their cognate amino acids. The encoded protein is a component of the multi-tRNA synthetase complex and catalyzes the ligation of methionine to tRNA molecules. [provided by RefSeq, Jan 2011]'