OPA1 (NM_130832) Human Untagged Clone

CAT#: SC127414

OPA1 (untagged)-Human optic atrophy 1 (autosomal dominant) (OPA1), nuclear gene encoding mitochondrial protein, transcript variant 3


  "NM_130832" in other vectors (4)

Reconstitution Protocol

USD 1,920.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol OPA1
Synonyms BERHS; largeG; MGM1; MTDPS14; NPG; NTG
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for NM_130832, the custom clone sequence may differ by one or more nucleotides


ATGTGGCGACTACGTCGGGCCGCTGTGGCCTGTGAGGTCTGCCAGTCTTTAGTGAAACACAGCTCTGGAA
TAAAAGGAAGTTTACCACTACAAAAACTACATCTGGTTTCACGAAGCATTTATCATTCACATCATCCTAC
CTTAAAGCTTCAACGACCCCAATTAAGGACATCCTTTCAGCAGTTCTCTTCTCTGACAAACCTTCCTTTA
CGTAAACTGAAATTCTCTCCAATTAAATATGGCTACCAGCCTCGCAGGAATTTTTGGCCAGCAAGATTAG
CTACGAGACTCTTAAAACTTCGCTATCTCATACTAGGATCGGCTGTTGGGGGTGGCTACACAGCCAAAAA
GACTTTTGATCAGTGGAAAGATATGATACCGGACCTTAGTGAATATAAATGGATTGTGCCTGACATTGTG
TGGGAAATTGATGAGTATATCGATTTTGGTCACAAATTGGTTAGTGAAGTCATAGGAGCTTCTGACCTAC
TTCTCTTGTTAGGTTCTCCGGAAGAAACGGCGTTTAGAGCAACAGATCGTGGATCTGAAAGTGACAAGCA
TTTTAGAAAGGTGTCAGACAAAGAGAAAATTGACCAACTTCAGGAAGAACTTCTGCACACTCAGTTGAAG
TATCAGAGAATCTTGGAACGATTAGAAAAGGAGAACAAAGAATTGAGAAAATTAGTATTGCAGAAAGATG
ACAAAGGCATTCATCATAGAAAGCTTAAGAAATCTTTGATTGACATGTATTCTGAAGTTCTTGATGTTCT
CTCTGATTATGATGCCAGTTATAATACGCAAGATCATCTGCCACGGGTTGTTGTGGTTGGAGATCAGAGT
GCTGGAAAGACTAGTGTGTTGGAAATGATTGCCCAAGCTCGAATATTCCCAAGAGGATCTGGGGAGATGA
TGACACGTTCTCCAGTTAAGGTGACTCTGAGTGAAGGTCCTCACCATGTGGCCCTATTTAAAGATAGTTC
TCGGGAGTTTGATCTTACCAAAGAAGAAGATCTTGCAGCATTAAGACATGAAATAGAACTTCGAATGAGG
AAAAATGTGAAAGAAGGCTGTACCGTTAGCCCTGAGACCATATCCTTAAATGTAAAAGGCCCTGGACTAC
AGAGGATGGTGCTTGTTGACTTACCAGGTGTGATTAATACTGTGACATCAGGCATGGCTCCTGACACAAA
GGAAACTATTTTCAGTATCAGCAAAGCTTACATGCAGAATCCTAATGCCATCATACTGTGTATTCAAGAT
GGATCTGTGGATGCTGAACGCAGTATTGTTACAGACTTGGTCAGTCAAATGGACCCTCATGGAAGGAGAA
CCATATTCGTTTTGACCAAAGTAGACCTGGCAGAGAAAAATGTAGCCAGTCCAAGCAGGATTCAGCAGAT
AATTGAAGGAAAGCTCTTCCCAATGAAAGCTTTAGGTTATTTTGCTGTTGTAACAGGAAAAGGGAACAGC
TCTGAAAGCATTGAAGCTATAAGAGAATATGAAGAAGAGTTTTTTCAGAATTCAAAGCTCCTAAAGACAA
GCATGCTAAAGGCACACCAAGTGACTACAAGAAATTTAAGCCTTGCAGTATCAGACTGCTTTTGGAAAAT
GGTACGAGAGTCTGTTGAACAACAGGCTGATAGTTTCAAAGCAACACGTTTTAACCTTGAAACTGAATGG
AAGAATAACTATCCTCGCCTGCGGGAACTTGACCGGAATGAACTATTTGAAAAAGCTAAAAATGAAATCC
TTGATGAAGTTATCAGTCTGAGCCAGGTTACACCAAAACATTGGGAGGAAATCCTTCAACAATCTTTGTG
GGAAAGAGTATCAACTCATGTGATTGAAAACATCTACCTTCCAGCTGCGCAGACCATGAATTCAGGAACT
TTTAACACCACAGTGGATATCAAGCTTAAACAGTGGACTGATAAACAACTTCCTAATAAAGCAGTAGAGG
TTGCTTGGGAGACCCTACAAGAAGAATTTTCCCGCTTTATGACAGAACCGAAAGGGAAAGAGCATGATGA
CATATTTGATAAACTTAAAGAGGCTGTTAAGGAAGAAAGTATTAAACGACACAAGTGGAATGACTTTGCG
GAGGACAGCTTGAGGGTTATTCAACACAATGCTTTGGAAGACCGATCCATATCTGATAAACAGCAATGGG
ATGCAGCTATTTATTTTATGGAAGAGGCTCTGCAGGCTCGTCTCAAGGATACTGAAAATGCAATTGAAAA
CATGGTGGGTCCAGACTGGAAAAAGAGGTGGTTATACTGGAAGAATCGGACCCAAGAACAGTGTGTTCAC
AATGAAACCAAGAATGAATTGGAGAAGATGTTGAAATGTAATGAGGAGCACCCAGCTTATCTTGCAAGTG
ATGAAATAACCACAGTCCGGAAGAACCTTGAATCCCGAGGAGTAGAAGTAGATCCAAGCTTGATTAAGGA
TACTTGGCATCAAGTTTATAGAAGACATTTTTTAAAAACAGCTCTAAACCATTGTAACCTTTGTCGAAGA
GGTTTTTATTACTACCAAAGGCATTTTGTAGATTCTGAGTTGGAATGCAATGATGTGGTCTTGTTTTGGC
GTATACAGCGCATGCTTGCTATCACCGCAAATACTTTAAGGCAACAACTTACAAATACTGAAGTTAGGCG
ATTAGAGAAAAATGTTAAAGAGGTATTGGAAGATTTTGCTGAAGATGGTGAGAAGAAGATTAAATTGCTT
ACTGGTAAACGCGTTCAACTGGCGGAAGACCTCAAGAAAGTTAGAGAAATTCAAGAAAAACTTGATGCTT
TCATTGAAGCTCTTCATCAGGAGAAATAA


>OriGene 5' read for NM_130832 unedited
NGCCTCACGATAGTAAACCATTCACTATAGGCGGCCGCGCAATTCGCACGAGGCGGACCG
TTCGTGCTGCCCGCCTANAAAGGTGAAGTGGTTGTTTCCGTGACGGACTGAGTACGGGTG
CCTGTCAGGCTCTTGCGGAAGTCCATGCGCCATTGGGAGGGCCTCGGCCGCGGCTCTGTG
CCCTTGCTGCTGAGGGCCACTTCCTGGGTCATTCCTGGACCGGGAGCCGGGCTGGGGCTC
ACACGGGGGCTCCCGCGTGGCCGTCTCGGCGCCTGCGTGACCTCCCCGCCGGCGGGATGT
GGCGACTACGTCGGGCCGCTGTGGCCTGTGAGGTCTGCCAGTCTTTAGTGAAACACAGCT
CTGGAATAAAAGGAAGTTTACCACTACAAAAACTACATCTGGTTTCACGAAGCATTTATC
ATTCACATCATCCTACCTTAAAGCTTCAACGACCCCAATTAAGGACATCCTTTCAGCAGT
TCTCTTCTCTGACAAACCTTCCTTTACGTAAACTGAAATTCTCTCCAATTAAATATGGCT
ACCAGCCTCGCAGGAATTTTTGGCCAGCAAGATTAGCTACGAGACTCTTAAAACTTCGCT
ATCTCATACTANGATCGGCTGTTGGGGGTGGCTACACAGCCCAAAAGACTTTTGATCAGT
GGAAAGATATGATACCCGGACCTTAGTGAAATTAAATGGATGGTGCCCTGACATTGTGTG
GGGAATTTGATGAGTATATCGATTTTGAGGAAATTAAAAAACCCCTTCCTAAGTTAAAAA
GAACTTGGTAAAGTTAGCCCCAGACTTTTGACAAGATTGTTGAAAACCCCTAACTATTGA
AAGAACTTTTTTACCCAAGGTTCTCCGGAAGAAAACGGGGTTTAAAGGAAACAATTCGTG
GGATTTGAAAGATAAAAG
>OriGene 3' read for NM_130832 unedited
GTACCTGTGCCGCAATCNANNATTGAGTTATTTTCTTTTTTTTTTTTTTAAAATCAAATT
TTCACACAATGGACACTTTTATTAACAAAGTAAAGGCTTAGTGAAAAAGCAGCAAATGAG
ATAGACATTTTAAGTAAAGAAAGCATTTCTTTGCTTGAATGTCCTTTAGCCAATTAAAAA
ATATTTATTTAAAACACATGAAAATGTTGTGATTGTAGTATTGTAATTAAATTGCAATAA
ATTGAACTAAATTTTTCTTTATTCAGCTGAGAGGGGGATGGTCAACATTACTTTAATTGT
TGAAATAATTTCTATTTAAAATAGTCTTATGAAAGAAAGCCTTTTTCTGATACCTAGTTG
AAATGTTTGATAATATTCTAAATGAAATTAGTCAATTGATTATCCTTAATCTTCCTCATC
CTGTGTCATAATCCGGTGGCTCTGTCAACAGGAGAAAAACCACTATTAACAGAAAGCCTA
ACGTAAGTATACAACATCTAAAATAAATCAATTTCAAATGGATATAGAGAGTGAGAAAAC
AGCAACTGAATCAAATAGGTAAGCTGGTACTGACTTGGCTTCCAAGGCAAATTTCTTCGC
TGGCTATGAAGGGTTGAAATTTCTTTCACTGAACGTTANCATCCAAATTAANCCTGTTTA
GGGGTTCTTCTGTTACCCATTTTTCTTTCATGTGGTGAATATAAACTGAATATCATGGTC
AAATAGTTTCTGGGAATAGGATACTACNGACAATACCATGATTAGCAGGCTACTGTGATT
TTATATACATTGATAACTTTAAACATTTGCTATATAGGTTTATTTTCAACACCTAGCATT
GGATTTCTGGAAAAAAACTGTACATGGGCACCTCCGTTAATCATAAACAGTGGCCCCCAA
ATAGACACAACAGAAACAAGCTCTTATCTCAGACTGACCTATGGCTTACATTTAATATTC
GATTGGCCTTAGC
Restriction Sites NotI-NotI     
ACCN NM_130832
Insert Size 4700 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_130832.1, NP_570845.1
RefSeq Size 6291 bp
RefSeq ORF 6291 bp
Locus ID 4976
Cytogenetics 3q29
Gene Summary 'The protein encoded by this gene is a nuclear-encoded mitochondrial protein with similarity to dynamin-related GTPases. The encoded protein localizes to the inner mitochondrial membrane and helps regulate mitochondrial stability and energy output. This protein also sequesters cytochrome c. Mutations in this gene have been associated with optic atrophy type 1, which is a dominantly inherited optic neuropathy resulting in progressive loss of visual acuity, leading in many cases to legal blindness. [provided by RefSeq, Aug 2017]'
Transcript Variant: This variant (3) is missing exon 4, but contains exon 4b, compared to transcript variant 1. It however, maintains the same reading frame and encodes an isoform (3) of 942 aa. This variant is based on an alternate splice pattern characterized by Delettre et al (2001, PMID: 11810270), but the complete 5' to 3' exon combination is inferred and not supported at the time of review by a single long cDNA. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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