PYK2 (PTK2B) (NM_173175) Human Untagged Clone

CAT#: SC127421

PTK2B (untagged)-Human PTK2B protein tyrosine kinase 2 beta (PTK2B), transcript variant 4

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PTK2B
• Synonyms: CADTK; CAKB; FADK2; FAK2; PKB; PTK; PYK2; RAFTK
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_173175, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTCTGGGGTGTCCGAGCCCCTGAGTCGAGTAAAGTTGGGCACGTTACGCCGGCCTGAAGGCCCTGCAG
  AGCCCATGGTGGTGGTACCAGTAGATGTGGAAAAGGAGGACGTGCGTATCCTCAAGGTCTGCTTCTATAG
  CAACAGCTTCAATCCTGGGAAAAACTTCAAACTGGTCAAATGCACTGTCCAGACGGAGATCCGGGAGATC
  ATCACCTCCATCCTGCTGAGCGGGCGGATCGGGCCCAACATCCGGTTGGCTGAGTGCTATGGGCTGAGGC
  TGAAGCACATGAAGTCCGATGAGATCCACTGGCTGCACCCACAGATGACGGTGGGTGAGGTGCAGGACAA
  GTATGAGTGTCTGCACGTGGAAGCCGAGTGGAGGTATGACCTTCAAATCCGCTACTTGCCAGAAGACTTC
  ATGGAGAGCCTGAAGGAGGACAGGACCACGCTGCTCTATTTTTACCAACAGCTCCGGAACGACTACATGC
  AGCGCTACGCCAGCAAGGTCAGCGAGGGCATGGCCCTGCAGCTGGGCTGCCTGGAGCTCAGGCGGTTCTT
  CAAGGATATGCCCCACAATGCACTTGACAAGAAGTCCAACTTCGAGCTCCTAGAAAAGGAAGTGGGGCTG
  GACTTGTTTTTCCCAAAGCAGATGCAGGAGAACTTAAAGCCCAAACAGTTCCGGAAGATGATCCAGCAGA
  CCTTCCAGCAGTACGCCTCGCTCAGGGAGGAGGAGTGCGTCATGAAGTTCTTCAACACTCTCGCCGGCTT
  CGCCAACATCGACCAGGAGACCTACCGCTGTGAACTCATTCAAGGATGGAACATTACTGTGGACCTGGTC
  ATTGGCCCTAAAGGGATCCGCCAGCTGACTAGTCAGGACGCAAAGCCCACCTGCCTGGCCGAGTTCAAGC
  AGATCAGGTCCATCAGGTGCCTCCCGCTGGAGGAGGGCCAGGCAGTACTTCAGCTGGGCATTGAAGGTGC
  CCCCCAGGCCTTGTCCATCAAAACCTCATCCCTAGCAGAGGCTGAGAACATGGCTGACCTCATAGACGGC
  TACTGCCGGCTGCAGGGTGAGCACCAAGGCTCTCTCATCATCCATCCTAGGAAAGATGGTGAGAAGCGGA
  ACAGCCTGCCCCAGATCCCCATGCTAAACCTGGAGGCCCGGCGGTCCCACCTCTCAGAGAGCTGCAGCAT
  AGAGTCAGACATCTACGCAGAGATTCCCGACGAAACCCTGCGAAGGCCCGGAGGTCCACAGTATGGCATT
  GCCCGTGAAGATGTGGTCCTGAATCGTATTCTTGGGGAAGGCTTTTTTGGGGAGGTCTATGAAGGTGTCT
  ACACAAATCACAAAGGGGAGAAAATCAATGTAGCTGTCAAGACCTGCAAGAAAGACTGCACTCTGGACAA
  CAAGGAGAAGTTCATGAGCGAGGCAGTGATCATGAAGAACCTCGACCACCCGCACATCGTGAAGCTGATC
  GGCATCATTGAAGAGGAGCCCACCTGGATCATCATGGAATTGTATCCCTATGGGGAGCTGGGCCACTACC
  TGGAGCGGAACAAGAACTCCCTGAAGGTGCTCACCCTCGTGCTGTACTCACTGCAGATATGCAAAGCCAT
  GGCCTACCTGGAGAGCATCAACTGCGTGCACAGGGACATTGCTGTCCGGAACATCCTGGTGGCCTCCCCT
  GAGTGTGTGAAGCTGGGGGACTTTGGTCTTTCCCGGTACATTGAGGACGAGGACTATTACAAAGCCTCTG
  TGACTCGTCTCCCCATCAAATGGATGTCCCCAGAGTCCATTAACTTCCGACGCTTCACGACAGCCAGTGA
  CGTCTGGATGTTCGCCGTGTGCATGTGGGAGATCCTGAGCTTTGGGAAGCAGCCCTTCTTCTGGCTGGAG
  AACAAGGATGTCATCGGGGTGCTGGAGAAAGGAGACCGGCTGCCCAAGCCTGATCTCTGTCCACCGGTCC
  TTTATACCCTCATGACCCGCTGCTGGGACTACGACCCCAGTGACCGGCCCCGCTTCACCGAGCTGGTGTG
  CAGCCTCAGTGACGTTTATCAGATGGAGAAGGACATTGCCATGGAGCAAGAGAGGAATGCTCGCTACCGA
  ACCCCCAAAATCTTGGAGCCCACAGCCTTCCAGGAACCCCCACCCAAGCCCAGCCGACCTAAGTACAGAC
  CCCCTCCGCAAACCAACCTCCTGGCTCCAAAGCTGCAGTTCCAGGAGGAGGACTTCATCCAACCCAGCAG
  CCGAGAAGAGGCCCAGCAGCTGTGGGAGGCTGAAAAGGTCAAAATGCGGCAAATCCTGGACAAACAGCAG
  AAGCAGATGGTGGAGGACTACCAGTGGCTCAGGCAGGAGGAGAAGTCCCTGGACCCCATGGTTTATATGA
  ATGATAAGTCCCCATTGACGCCAGAGAAGGAGGTCGGCTACCTGGAGTTCACAGGGCCCCCACAGAAGCC
  CCCGAGGCTGGGCGCACAGTCCATCCAGCCCACAGCTAACCTGGACCGGACTGATGACCTGGTGTACCTC
  AATGTCATGGAGCTGGTGCGGGCCGTGCTGGAGCTCAAGAATGAGCTCTGTCAGCTGCCCCCCGAGGGCT
  ACGTGGTGGTGGTGAAGAATGTGGGGCTGACCCTGCGGAAGCTCATCGGGAGCGTGGATGATCTCCTGCC
  TTCCTTGCCGTCATCTTCACGGACAGAGATCGAGGGCACCCAGAAACTGCTCAACAAAGACCTGGCAGAG
  CTCATCAACAAGATGCGGCTGGCACAGCAGAACGCCGTGACCTCCCTAAGTGAGGAGTGCAAGAGGCAGA
  TGCTGACGGCTTCACACACCCTGGCTGTGGACGCCAAGAACCTGCTCGACGCTGTGGACCAGGCCAAGGT
  TCTGGCCAATCTGGCCCACCCACCTGCAGAGTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_173174 unedited
  AATTACACCCGCCCGTTGACGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAA
  GCAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCG
  CGAATTCGGCACGAGGCCACAGCCTCCGGAGCCGTTGCACACCTACCTGCCCGGCCGACT
  TACCTGTACTTGCCGCCGTCCCGGCTCACCTGGCGGTGCCCGAGGAGTAGTCGCTGGAGT
  CCGCGCCTCCCTGGGACTGCAATGTGCCGATCTTAGCTGCTGCCTGAGAGGATGTCTGGG
  GTGTCCGAGCCCCTGAGTCGAGTAAAGTTGGGCACGTTACGCCGGCCTGAAGGCCCTGCA
  GAGCCCATGGTGGTGGTACCAGTAGATGTGGAAAAGGAGGACGTGCGTATCCTCAAGGTC
  TGCTTCTATAGCAACAGCTTCAATCCTGGGAAAAACTTCAAACTGGTCAAATGCACTGTC
  CAGACGGAGATCCGGGAGAGCATCACCTCCATCCTGCTGAGCGGGCGGATCGGGCCCAAC
  ATCCGGTTGGCTGAGTGCTATGGGCTGAGGCTGAACACATGAAGTCCGATGAGATCCACT
  GGCTGCACCCACAGAGACGGTGGGTTGAGGGTGCAGGACACGTATTGAGTGTCTGCACGT
  GGACGCCGGTTGGTNAGGTATGACCTTCACATCCGCTACTTGCCAGAAGACTTCATGGAG
  AGCCTGACAGGAAGGACAGGACCACGCTGCTCTATTTTTACCAACAGCTCCGGAACGACT
  ACATGCAGCGCTCGTCCAGCAGGGTCNAACGAGGGGCATGCCTGTGCAGCTTGGGCTGCC
  GGACGTCTTACAGGCGGTTCTTCCAAGGATATGCCTCACATGCCTGNACACAGAAGTCAC
  CTCGGCTTTGAAAAGGGAAGGGGGGGCTTGGAACTTGTCTTCCCAAGC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_173175 unedited
  ACTGTGGACCCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCAAATGGGA
  CAATTTTAATTCAACCACAAGTCAAATAGAAAGAAGTTAAAAGAATGTTTATGCAAACAC
  ATGAGAAAAGAAGGGTGCAGATGAGAATGGGGGTTGGGGAGAGAAAGAGGAGGAGTAAGA
  AAAGAGGGAAAAGCAAGGGAAAGTAAAGGAAGAAAGAGAAAGAGGGGCAGGAAGAGAGCG
  GATTTGGCCCAAGGTCCTATCTTGGCCGCATCTCTCTGCTTCTTCCCCCTGATGCTTGGT
  TTGTTGACAACACAGCATCCTGTGCCTGGACTCCCAATTAGCTTGTTCCTGGACTGTGCC
  CCAGGTCCTCCCTCAGGAGGGCACATGCTGTCAGTCCAGACCAAACTCACATTAAATAAA
  TTTCAATATACACTGTACAAGAATGCCAGGCCCATCCCTCATCTCACTGGCTGCCTGACC
  CCCAAAACAAAGCTCCTCCCCAGCTTCTCTGTGCATCAAGGGACATCTGAGGGCCGAAGA
  GGAAGGGAAAGGAATAGCTTGGTTCCCAAATCGGCCTGCCATGTCCATATATAAAGCTAG
  GGGCACCATGTGACACCTCCCCACCCCCAGGAGCCCCCTGCATGGACCAGGCTGGCTTAG
  GGAGCAGTGGGCCAGCAGCAGCAGTCCCCCTCTGAGCCATCTTTGTCACCGTCACCCTGT
  GTCCATAGCCCAGAGTCCCCTCTGTCCAGCTGCAGCCTGAGCAGTACAGCCAGGGNTAGG
  GGTGGGGGAGAAGGGCGTCNTGCAAAGTGGNCAGGGAANGGTGACTCCCCTTGGCCTTCC
  CCCTGNAAGACCCACATGACCAACAGCAAGGCAGGGGAGGTACATGNGCAGCAGGGGCGG
  AAAGACGCAGCAGGTGGGCCCCACCCTCCGTCACTCTGCAGGTGGTC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_173175
• ORF Size: 2904 bp
• Insert Size: 4000
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_173175.1, NP_775267.1
• RefSeq Size: 3936
• RefSeq ORF: 2904
• Locus ID: 2185
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Calcium signaling pathway, Chemokine signaling pathway, GnRH signaling pathway, Leukocyte transendothelial migration, Natural killer cell mediated cytotoxicity
• Gene Summary: This gene encodes a cytoplasmic protein tyrosine kinase which is involved in calcium-induced regulation of ion channels and activation of the map kinase signaling pathway. The encoded protein may represent an important signaling intermediate between neuropeptide-activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. The encoded protein undergoes rapid tyrosine phosphorylation and activation in response to increases in the intracellular calcium concentration, nicotinic acetylcholine receptor activation, membrane depolarization, or protein kinase C activation. This protein has been shown to bind CRK-associated substrate, nephrocystin, GTPase regulator associated with FAK, and the SH2 domain of GRB2. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Four transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (4) lacks an alternate in-frame exon compared to variant 1, resulting in a shorter isoform (b) compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.