RAP2B (NM_002886) Human Untagged Clone

CAT#: SC127520

RAP2B (untagged)-Human RAP2B, member of RAS oncogene family (RAP2B)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RAP2B
• Synonyms: MGC20484
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC127520 sequence for NM_002886 edited (data generated by NextGen Sequencing)
  ATGAGAGAGTACAAAGTGGTGGTGCTGGGCTCGGGCGGCGTGGGCAAGTCCGCGCTCACC
  GTGCAGTTCGTGACGGGCTCCTTCATCGAGAAGTACGACCCGACCATCGAAGACTTTTAC
  CGCAAGGAGATTGAGGTGGACTCGTCGCCGTCGGTGCTGGAGATCCTGGATACGGCGGGC
  ACCGAGCAGTTCGCGTCCATGCGGGACCTGTACATCAAGAACGGCCAGGGCTTCATCCTG
  GTCTACAGCCTCGTCAACCAGCAGAGCTTCCAGGACATCAAGCCCATGCGGGACCAGATC
  ATCCGCGTGAAGCGGTACGAGCGCGTGCCCATGATCCTGGTGGGCAACAAGGTGGACCTG
  GAGGGTGAGCGCGAGGTCTCGTACGGGGAGGGCAAGGCCCTGGCTGAGGAGTGGAGCTGC
  CCCTTCATGGAGACGTCGGCCAAAAACAAAGCCTCGGTAGACGAGCTATTTGCCGAGATC
  GTGCGGCAGATGAACTACGCGGCGCAGCCCAACGGCGATGAGGGCTGCTGCTCGGCCTGC
  GTGATCCTCTGA
  
  Clone variation with respect to NM_002886.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002886 unedited
  NNGGGCGTCACATTTGTATACGACTCATATAGGCGGCCGCGNAATTCGCACCAGCCGCGG
  CGGTGGGCGCACGTTCCGCGGGGACTCATGCCACGCGCGTCCCGGCCCGACGCGCAATTA
  GCAGCCACCTCCGCAGCCCGCCGCCACCGCCTCCCTGCCCTCCCGGGCTGCCGCAGCTAG
  GAGCTCCAGCCGTCGCCTCGCGCAGGCTGCGGGCATTGTCCTCTCGGTTCGCCGCCCGGG
  CTGCTACTGCCGCCGCGGACTGCTGCGGGGCCCGGACCCGCACCCCAGGGATACGCTGCC
  GCCGCCGCCGGCCGGCCCGGCGCCCGGCCTCCGTTCGGTGGTTTCCGCCCTGCGTTCTCT
  GGGTTGCTCTCTCCTGGGTTTTTCCTGCGTAGCTGAGGAAGGGGAAGAGAAGTCCAGCCG
  CCAAGCCCAGCCTTCCCCGGCGCGCAGCCCCGACGGGGCCGCGGCAGGCGCGGCGAGAGC
  GCTGACGGAGCCATGAGAGAGTACANAGTGGTGGTGCTGGGCTCGGGCGGCGTGGGCAAG
  TCCGCGCTCACCGTGCAGTTCGTGACGGGCTCCTTCATCGAGAAGTACGACCCGACCATC
  GAAGACTTTTACCGCAGGGAGATTTGAGTGGACTCGTCGCCGTCNGTGCTGGAGATCCTG
  GATACGGCGGGCACGGAGCAGTTCGCGTCCCTGCGGGGACCTGTACATCAAGAACGGCCA
  GGGCTTCATCCTGGTCTACAGCCTCGTCAACCAGCAAAGCTTCCAGGACATTAAGCCCAT
  GCGGGACCAGATCATTCGCGTGAAGCGGTACCAGCGCTTGCCCTTGATCCTGGTGGGCAC
  CAGGTTGAACTTGGAGGGTGGACCCCAGGCTTCCTACGGGGAGGCCAAGGCCCCGGCCTA
  AGAATGGAACC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002886 unedited
  GGGGGGCCNNNNCCCNTNTTTNCNNNNNNNNNTTATCNTAGNNCCNCGGNCCGCATACNA
  NGATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTCTTTAAAAAGACATTTGT
  ATTAAAAGTCTTAAAATGAAGAAGAAATGATTAAACGCATGAAATTAAAAATTACAATTA
  GTTAGAATTGGTACATCACAACAGTAAAATTTTGCTCTTTGCTTCTGGAGGAACACCCTA
  CAACAGGTAATTAAAAAATTTAAAAATAATATGAAAGGGGAGCTAAATACCTGAAAATTT
  AAACAAAATGAAGCAAAAAAAAGGGAAACAAATTCATTGCAAGAGATGGAAATTACATTT
  TAAAGGCTGAAAATAATAAAGAAATTCACTAAACAACAGGCCCTATTGTCCTGGCTCCTT
  CAGAAAGATTACTGAAAGGAAACTGGGAAGTGGTTTGGGGAATAGCAGATTCTGGGATAT
  GGTGGGAAGTTCACAGGTTGGCACCGGCTGGAACAGCTGAGTTTTGAAGGCACTCTGCAG
  ACACAGGGGTGCTGGGGGCCCCTGATCACATTTACAAAGTCTTTGTACTGTATTTTTTAA
  AGCTAGTCATTAAAATCGGTTACTGCCCACCAAAAAACAAAAACAAAGGCTAAACTTNAA
  NCACACACCCCCNAAACACATACACACACCCCCCTTGTACACACGAAAATGTGCAAATCT
  GTGACATTTTTTTTACAGCTGATAAAAAAAAAAATACTACCCATTTTCCCCAGACTTCCC
  TTTGATACACGAGGTAAACAAAATAGATTTTTTTTCCCCAATTCAAGCGGACCCTTCTGG
  CCCCCCAACTTGNTTGGCCATCAAGGGTAACAAGTCCAGTTCCAATGGGCAATCTTTAAA
  ATTGGATCACATGGACCANGAATTCCAATTTGCATAAACATNCCTAAAATACCAATCAGT
  CTTGGGGGTTGCCCCTCTCAGACTGGCAAAAAGGAAACCCCATCTAAATTTCCACTACTT
  CTGAANAAGNNGNAAANNNAGTTTTNCTTTCTCCTGC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002886
• Insert Size: 2440 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002886.2, NP_002877.2
• RefSeq Size: 6237 bp
• RefSeq ORF: 552 bp
• Locus ID: 5912
• Cytogenetics: 3q25.2
• Domains: ras, RAS, RHO, RAB
• Protein Families: Druggable Genome
• Gene Summary: 'This intronless gene belongs to a family of RAS-related genes. The proteins encoded by these genes share approximately 50% amino acid identity with the classical RAS proteins and have numerous structural features in common. The most striking difference between the RAP and RAS proteins resides in their 61st amino acid: glutamine in RAS is replaced by threonine in RAP proteins. Evidence suggests that this protein may be polyisoprenylated and palmitoylated. [provided by RefSeq, Jul 2008]'