ILF3 (NM_004516) Human Untagged Clone

CAT#: SC127534

ILF3 (untagged)-Human interleukin enhancer binding factor 3, 90kDa (ILF3), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ILF3
• Synonyms: CBTF; DRBF; DRBP76; MMP4; MPHOSPH4; MPP4; MPP4110; NF-AT-90; NF90; NF90a; NF90b; NF90c; NF90ctv; NF110; NF110b; NFAR; NFAR-1; NFAR-2; NFAR2; NFAR90; NFAR110; TCP80; TCP110
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC127534 sequence for NM_004516 edited (data generated by NextGen Sequencing)
  ATGCGTCCAATGCGAATTTTTGTGAATGATGACCGCCATGTGATGGCAAAGCATTCTTCC
  GTTTATCCAACACAAGAGGAGCTGGAGGCAGTCCAGAACATGGTGTCCCACACGGAGCGG
  GCGCTCAAAGCTGTGTCCGACTGGATAGACGAGCAGGAAAAGGGTAGCAGCGAGCAGGCA
  GAGTCCGATAACATGGATGTGCCCCCAGAGGACGACAGTAAAGAAGGGGCTGGGGAACAG
  AAGACGGAGCACATGACCAGAACCCTGCGGGGAGTGATGCGGGTGGGCCTGGTGGCAAAG
  GGCCTCCTACTCAAGGGGGACTTGGATCTGGAGCTGGTGCTGCTGTGTAAGGAGAAGCCC
  ACAACCGCCCTCCTGGACAAGGTGGCCGACAACCTGGCCATCCAGCTTGCTGCTGTAACA
  GAAGACAAGTACGAAATACTGCAATCTGTCGACGATGCTGCGATTGTGATAAAAAACACA
  AAAGAGCCTCCATTGTCCCTGACCATCCACCTGACATCCCCTGTTGTCAGAGAAGAAATG
  GAGAAAGTATTAGCTGGAGAAACGCTATCAGTCAACGACCCCCCGGACGTTCTGGACAGG
  CAGAAATGCCTTGCTGCCTTGGCGTCCCTCCGACACGCCAAGTGGTTCCAGGCCAGAGCC
  AACGGGCTGAAGTCTTGTGTCATTGTGATCCGGGTCTTGAGGGACCTGTGCACTCGCGTG
  CCCACCTGGGGTCCCCTCCGAGGCTGGCCTCTCGAGCTCCTGTGTGAGAAATCCATTGGC
  ACGGCCAACAGACCGATGGGTGCTGGCGAGGCCCTGCGGAGAGTGCTGGAGTGCCTGGCG
  TCGGGCATCGTGATGCCAGATGGTTCTGGCATTTATGACCCTTGTGAAAAAGAAGCCACT
  GATGCTATTGGGCATCTAGACAGACAGCAACGGGAAGATATCACACAGAGTGCGCAGCAC
  GCACTGCGGCTCGCTGCCTTCGGCCAGCTCCATAAAGTCCTAGGCATGGACCCTCTGCCT
  TCCAAGATGCCCAAGAAACCAAAGAATGAAAACCCAGTGGACTACACCGTTCAGATCCCA
  CCAAGCACCACCTATGCCATTACGCCCATGAAACGCCCAATGGAGGAGGACGGGGAGGAG
  AAGTCGCCCAGCAAAAAGAAGAAGAAGATTCAGAAGAAAGAGGAGAAGGCAGAGCCCCCC
  CAGGCTATGAATGCCCTGATGCGGTTGAACCAGCTGAAGCCAGGGCTGCAGTACAAGCTG
  GTGTCCCAGACTGGGCCCGTCCATGCCCCCATCTTTACCATGTCTGTGGAGGTTGATGGC
  AATTCATTCGAGGCCTCTGGGCCCTCCAAAAAGACGGCCAAGCTGCACGTGGCCGTTAAG
  GTGTTACAGGACATGGGCTTGCCGACGGGTGCTGAAGGCAGGGACTCGAGCAAGGGGGAG
  GACTCGGCTGAGGAGACCGAGGCGAAGCCAGCAGTGGTGGCCCCTGCCCCAGTGGTAGAA
  GCTGTCTCCACCCCTAGTGCGGCCTTTCCCTCAGATGCCACTGCCGAGCAGGGGCCGATC
  CTGACAAAGCACGGCAAGAACCCAGTCATGGAGCTGAACGAGAAGAGGCGTGGGCTCAAG
  TACGAGCTCATCTCCGAGACCGGGGGCAGCCACGACAAGCGCTTCGTCATGGAGGTCGAA
  GTGGATGGACAGAAGTTCCAAGGTGCTGGTTCCAACAAAAAGGTGGCGAAGGCCTACGCT
  GCTCTTGCTGCCCTAGAAAAGCTTTTCCCTGACACCCCTCTCGCCCTTGATGCCAACAAA
  AAGAAGAGAGCCCCAGTACCCGTCAGAGGGGGACCGAAATTTGCTGCTAAGCCACATAAC
  CCTGGCTTCGGCATGGGAGGCCCCATGCACAACGAAGTGCCCCCACCCCCCAACCTTCGA
  GGGCGGGGAAGAGGCGGGAGCATCCGGGGACGAGGGCGCGGGCGAGGATTTGGTGGCGCC
  AACCATGGAGGCTACATGAATGCCGGTGCTGGGTATGGAAGCTATGGGTACGGAGGCAAC
  TCKGCGACAGCAGGCTACAGTGACTTTTTCACAGACTGCTACGGCTATCATGATTTTGGG
  TCTTCCTAG
  
  Clone variation with respect to NM_004516.3
  2043 g=>k
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004516 unedited
  ACGACTCACTATGGGCGGCCGCGAATTCGGCACGAGGGAGAGAGAGAGAGAGAGAGAGAG
  AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG
  AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGGCCCGGCGCGCA
  CCGCGTGGCTTTTGGGGGCACACCCCGGCGGGCTGTGGCAGGAGGGCGGCGGCGGCGGCT
  GCGGTCGAAGAAGGGGACGCCGACAAGAGTTGAAGTATTGATAACACCAAGGAACTCTAT
  CACAATTTGAAAAGATAAGCAAAAGTTTGATTTCCAGACACTACAGAAGAAGTAAAAATG
  CGTCCAATGCGAATTTTTGTGAATGATGACCGCCATGTGATGGCAAAGCATTCTTCCGTT
  TATCCAACACAAGAGGAGCTGGAGGCAGTCCAGAACATGGTGTCCCACACGGAGCGGGCG
  CTCAAAGCTGTGTCCGACTGGATAGACGAGCAGGAAAAGGGTAGCAGCGAGCAGGCAGAG
  TCCGATAACATGGATGTGCCCCCAGAGGACGACAGTAAAGAAGGGGCTGGGGGACANNAG
  ACGGAGCACATGACCAGAACCCTGCGGGGAGTGATGCCCGGTGGGGCCTGGTGGCANAGG
  GNCCCTTCTACTNCAGGNGGGGACTTGNATCTNGNAGCTGGTGNCCTGCTGTGTAAAGGA
  GAAGCCCACCACCCGCCCTCCTGTACAGAGTGGGCCGACACCTGGCCATCCCAGCTTGTT
  GCTGGTACAGAAGACAAGTACCAAATACTGGCATCTGTCNACGATGCTTGCGATTGTGAT
  AAAAACACAAAAGAGCCTCCATTGTCCCTGACCATCCACCTGGACATCCCCTGTTGTCAN
  AGAAAAAAGGGAGAAAGTATTTNCTGGAGAAACGCTACAGNTCACGACCCCCCCGGACGT
  TTTGACAGGCAAATGTCTTGTTCGCTTTGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004516 unedited
  GCGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTTTTGCTTGGTACTTTCACAT
  GTATTGATTAAAAAAAAATAAAGGGGAGGGGGAGTTTTCAACGTCATTCAGGATAACTAT
  TTTCCTGGTATAAAAAGACATTATTCTCAAACAATAACTTTCTAAAAAAAAAATACATCA
  TGCAATCAAAGTTTTAAAAAGTTTCACTGAATGTTTCAAACCTCTGGGAACAAATCATGT
  AGTAACCATTTTGACCAGTTTTTGCCTGAATAAACGCAAAATCTTGCAAGTCAACACAGA
  TGCAGGCTTTGCTGCAAAAAGAACCGCTGATCAGAGCCAAAACCAGCTTCCAAGGAGGGC
  ACCTGCCATAAAACGGGGCCTCAGCAGAGAGCCACAATGACCCCCTGTGTTGCGCCAAAC
  TTTTACCCCCTTGACACCTGTGGCACCTCGTCACCCTGTTCCCCCCCATTATGATCTCCT
  ACCCCCCCCGTTCCCGTGGCCGCGCTCCGCCCCCACGATTATCCCCCCCGACCCTCCCCC
  CGCCTCCACTTCCCGACCCCGTCCCTCCCTCACCACCCCCCCCCCCATCTCCCCCATCTC
  TCTCCCCCCTTCCCCTTCCCATCTTCGATTTACCCCCCCCCCCCTTACTCTCCCTGCCTA
  CTCCACCCACTATATAGCTCTCTCTACCCCCCATTCCCCCCCCCCCGCACCCCTTCCCCC
  TTCATCCCTCCATTTTTCCCCCCCCTCCTCCCCCCTCCCCCCTCTCACCAACGCCTCCCC
  CTCCCACATCCCTTCCTCCCCCCCGTCACCCGGGGCTCTACCCCCTACGATACCCCCCAC
  TCACGTCGACCCTATCTGTTTTCCCCACCCCTCGCGATAGACTCCCTCGATCCCTCGTCC
  CCTTCATCCCCTCCCCTCCTCCTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004516
• Insert Size: 3670 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004516.2, NP_004507.2
• RefSeq Size: 3677 bp
• RefSeq ORF: 2109 bp
• Locus ID: 3609
• Cytogenetics: 19p13.2
• Domains: DSRM, DZF
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: 'This gene encodes a double-stranded RNA (dsRNA) binding protein that complexes with other proteins, dsRNAs, small noncoding RNAs, and mRNAs to regulate gene expression and stabilize mRNAs. This protein (NF90, ILF3) forms a heterodimer with a 45 kDa transcription factor (NF45, ILF2) required for T-cell expression of interleukin 2. This complex has been shown to affect the redistribution of nuclear mRNA to the cytoplasm. Knockdown of NF45 or NF90 protein retards cell growth, possibly by inhibition of mRNA stabilization. In contrast, an isoform (NF110) of this gene that is predominantly restricted to the nucleus has only minor effects on cell growth when its levels are reduced. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Dec 2014]'
  Transcript Variant: This variant (2) differs in the 3' UTR and coding region compared to variant 4. The resulting isoform (b) has a distinct C-terminus compared to isoform d. This isoform is also called NFAR-1 or DRBP76.