NME4 (NM_005009) Human Untagged Clone

CAT#: SC127542

NME4 (untagged)-Human non-metastatic cells 4, protein expressed in (NME4), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NME4
• Synonyms: NDPK-D; nm23-H4; NM23H4
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_005009, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGGCGGCCTCTTCTGGCGCTCCGCGCTGCGGGGGCTGCGCTGCGGCCCGCGGGCCCCGGGCCCGAGCC
  TGCTAGTGCGCCACGGCTCGGGAGGGCCCTCCTGGACCCGGGAGCGGACCCTGGTGGCGGTGAAGCCCGA
  TGGCGTGCAACGGCGGCTCGTTGGGGACGTGATCCAGCGCTTTGAGAGGCGGGGCTTCACGCTGGTGGGG
  ATGAAGATGCTGCAGGCACCAGAGAGCGTCCTTGCCGAGCACTACCAGGACCTGCGGAGGAAGCCCTTCT
  ACCCTGCCCTCATCCGCTACATGAGCTCTGGGCCTGTGGTGGCCATGGTCTGGGAAGGGTACAATGTCGT
  CCGCGCCTCGAGGGCCATGATTGGACACACCGACTCGGCTGAGGCTGCCCCAGGAACCATAAGGGGTGAC
  TTCAGCGTCCACATCAGCAGGAATGTCATCCACGCCAGCGACTCCGTGGAGGGGGCCCAGCGGGAGATCC
  AGCTGTGGTTCCAGAGCAGTGAGCTGGTGAGCTGGGCAGACGGGGGCCAGCACAGCAGCATCCACCCAGC
  CTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005009 unedited
  GAGAGAGAGAGAGAGAGAGAGAGAGAGCGCGGGCCGGGCGTCATGGGCGGCCTCTTCTGG
  CGCTCCGCGCTGCGGGGGCTGCGCTGCGGCCCGCGGGCCCCGGGCCCGAGCCTGCTAGTG
  CGCCACGGCTCGGGAGGGCCCTCCTGGACCCGGGAGCGGACCCTGGTGGCGGTGAAGCCC
  GATGGCGTGCAACGGCGGCTCGTTGGGGACGTGATCCAGCGCTTTGAGAGGCGGGGCTTC
  ACGCTGGTGGGGATGAAGATGCTGCAGGCACCAGAGAGCGTCCTTGCCGAGCACTACCAG
  GACCTGCGGAGGAAGCCCTTCTACCCTGCCCTCATCCGCTACATGAGCTCTGGGCCTGTG
  GTGGCCATGGTCTGGGAAGGGTACAATGTCGTCCGCGCCTCAAGGGCCATGATTGGACAC
  ACCGACTCGGCTGAGGCTGCCCCAGGAACCATAAGGGGTGACTTCAGCGTNCACATCAGC
  AGGAATGTCATCCACGCCAGCGACTCCGTGGAGGGGGCCCAGCGGGAGATCCAGCTGTGG
  TTCCAGAGCANTGAGCTGGTGAGCTGGGCAGATGGGGGCCAGCACAGCAGCATCCACCCC
  AGCCTGAGCTCAAGCTGNCCTTACCACCCCCATCCCCACGCAGGACAACTACCTCCGTCA
  GCAAGACCCAAGCCACATNCAAACTGNCTGNCCCAACCACTTACTTCCTGGTNACCTTGG
  CCCACCCCAGCCAGAAGGAGTTGACCACCAACTTATGGCTTTTGTCCCCAGCCCACACAG
  AATGGACATTCTTTTTGACAAAGGCCGGCAACCTTGGGGGGGGGGGGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005009 unedited
  NAACTTGAACCCGGCCGCATCTANGATCGGTTTTTTTTTTTTTTTTTTGGGTGTGCAAAA
  CTTCCTCTTTACTGTTCAACTCCTTACAACTTGCATAAAGGCGTCATTTCTACCTCAGAG
  CTGGGGGAAGTCATAATTAGTGTACCACCCATGTGCTGGGCACAGTGAATTTTAATGCCT
  CCCCTTTAGAGGAGAGGTTATGATAATGTGAAGACCCCCCCCCACCACAAAGGTTGTCCG
  GCACTTTGGTGCAAAAAGGATTGGTCCAATCTTGTGCTGGCTTGGGGTACAGAAAGGCAC
  TGAAGTTGGTGGCTCAAACTCCTCTGGGCTGGGGTGGGGCANAGGTGAACAGGGAAGTAA
  GTGGTTTGGGACAGGCAGGTTTGGATGTGGGCTTGGGTTCTTGCTGACGGAGGTAGTTGG
  TCCTGCGTGGGGGATGGGGTGGTAAGGGCAGCTTGAGCCTCACGCTGGGTGGATGCTGCT
  GTGCTGGCCCCCATCTGCCCANCTCACAGCCCCACTGCGATGGAACCCCGCAGGATCTTC
  CCTTGGCCCCCCTGCCGGACCCCTCCGCATGCATACCACAACAAGTCGACGCGCAGCCTA
  AGCACCCCCTTCCTCCCCGCGACCGCGGTGTTCCTCTCGCATCCCCCGCCAACCCCTAAT
  TCGCCGCGTATTGGCCCCCCTCCTCACTGCCTTCCCTCTTTTTTATCCGCTCCGCTCCTA
  TATCCTTTTNCNCNCCNCCCCNCCNNTTCTTTTTCCCCTTCCTCTTTTTCTCCTTCCTTC
  TTCCCCCTCTCGTTCTGCTCCTTCCCCCCGCCCCCCGCTCTTCCTTGTTTGGCCGTTCCC
  TCCCTCCCCCTCCCCCCCCCTCTCCCGCACGTCTCCCCGCCCGCCCTCCGCGCCCCCCCT
  CCACGCTCCTCACTACACAACAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005009
• Insert Size: 1060 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005009.2, NP_005000.1
• RefSeq Size: 1059 bp
• RefSeq ORF: 564 bp
• Locus ID: 4833
• Cytogenetics: 16p13.3
• Domains: NDK
• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Purine metabolism, Pyrimidine metabolism
• Gene Summary: 'The nucleoside diphosphate (NDP) kinases (EC 2.7.4.6) are ubiquitous enzymes that catalyze transfer of gamma-phosphates, via a phosphohistidine intermediate, between nucleoside and dioxynucleoside tri- and diphosphates. The enzymes are products of the nm23 gene family, which includes NME4 (Milon et al., 1997 [PubMed 9099850]).[supplied by OMIM, May 2008]'
  Transcript Variant: This variant (1) encodes the longest isoform (a).