DDX11 (NM_030655) Human Untagged Clone

CAT#: SC127551

DDX11 (untagged)-Human DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 11 (CHL1-like helicase homolog, S. cerevisiae) (DDX11), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DDX11
• Synonyms: CHL1, KRG2, CHLR1, MGC9335
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_030655, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCTAATGAAACACAGAAGGTTGGTGCCATCCATTTTCCTTTTCCCTTCACACCCTATTCCATCCAGG
  AAGACTTCATGGCAGAGCTGTACCGGGTTTTGGAGGCTGGCAAGATTGGGATATTTGAGAGTCCAACTGG
  CACTGGGAAGTCCTTAAGTCTTATTTGTGGGGCCCTCTCTTGGCTCCGTGACTTTGAACAGAAGAAGCGT
  GAAGAAGAGGCACGACTCCTTGAAACTGGAACTGGCCCCTTACATGATGAGAAAGATGAATCCCTGTGTC
  TGTCTTCTTCCTGCGAAGGGGCTGCAGGCACCCCGAGGCCTGCTGGAGAACCGGCCTGGGTTACTCAGTT
  TGTGCAGAAGAAAGAAGAGAGGGACCTGGTGGACCGACTAAAGGCGGAGCAGGCCAGGAGGAAGCAGCGA
  GAAGAACGCCTGCAGCAGCTGCAGCACAGGGTGCAGCTCAAGTATGCAGCCAAGCGCCTGAGGCAGGAAG
  AAGAAGAAAGAGAGAATCTCCTCCGCCTCAGCAGGGAGATGCTAGAGACAGGCCCGGAGGCTGAGCGGCT
  GGAGCAGCTGGAGTCTGGGGAGGAGGAGCTGGTCCTCGCCGAATACGAGAGTGATGAGGAGAAAAAGGTG
  GCGAGCAGGGCGCCTTCTGATGCCACCTCCAGCCGCCATCCACCAGACGCCAGCTTCCCCGCTGCCCTGA
  ACTTCCTCCAGCGCACCAGGCCTTCCTCTGTCCTGTCTGAGGATTTGCTCATGCAACGTGCTGTGGCCAA
  ACACCCTGCCCTCCTCCCTTGGCAGATGTCTTCCTCTCCTTTAAGGCCTGGCTCAGAGTGGATGAGGATG
  AGGATGACCTGGAGGAAGAACACATAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_030655 unedited
  GCGGCCGCGAATTCGCACGAGGCCGTTTCTAACNAGTGGCGTTTGCCCTGATTCCCGGGG
  CCTGGCTTTCAGCGTAGCAATTCTGGCCGGCGAAGAAGGTGAGCGCAGTGCTGTGTGGCA
  GCAGAGCTCCTTAGGACGAGGAGCAGCGGGACGAGGAAGGGCAGACTGGTGAAATCGCAA
  ACTGGGCGTCTGTTCCGGNCGCCGGACCCCTATTTGCAAAGGTCCATGGCTAATGAAACA
  CAGAAGGTTGGTGCCATCCATTTTCCTTTTCCCTTCACACCCTATTCCATCCAGGAAGAC
  TTCATGGCAGAGCTGTACCGGGTTTTGGAGGCTGGCAAGATTGGGATATTTGAGAGTCCA
  ACTGGCACTGGGAAGTCCTTAAGTCTTATTTGTGGGGCCCTCTCTTGGCTCCGTGACTTT
  GAACAGAAGAAGCGTGAAGAAGAGGCACGACTCCTTGAAACTGGAACTGGCCCCTTACAT
  GATGAGAAAGATGAATCCCTGTGTCTGTCTTCTTCCTGCGAAGGGGCTGCAGGCACCCCG
  AGGCCTGCTGGAGAACCGGCCTGNGTTACTCAGTTTGTGCAGAAGAAAGAAGAGAGGGAC
  CTGGTGGACCGACTAAAGGCGGAGCAGGCCAGGAGGAAGCAGCGAGAAGAACGCCTGCAG
  CAGCTGCAGCACAGGGTGCAGCTCAAGTATGCAGCCAAGCGCCTTNGAGCAGAAGAAGAA
  GAAGAGAGATCTCCTCCGCCTCACAGGNAGTGCTANAACAGCCCNGAGCTGAGCGCTGAC
  ACTGGGNATTGGGGAGAAAGGACTGGTCTCGCGATACAGAGTGATAGAG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_030655 unedited
  TTTAGGTTCTATAGCTGTGCAGGATGCAAACATCTGGGCATCAGTGACTTTTCTCCTGCC
  CCCGTTTGTCCCTGATGGTGGCAGAAGATCCCAGCTGACCACAGCCCCTTTCTCTGGGGT
  CAGAGGGAGAAGACAGGCGCAGTCAGCATGGGCAGCTGTTGCAGGTGGGAGGGATATTTT
  CCCACAGGAACAAAGGTCTCCGTGATGACACGGGGTCTCTATAGTCATGTTGAGAGCCTA
  ACGGACCTTGGCATAATTGCTGGTGTTGGTGTAGAAGGTGCCTTGCAGTTTGCTCAAGTG
  GTTGAGAGGGAGGGAGGTGCCATAGACTTGGACGAACTGGCTCGAGTTTTAGCTCCATAT
  TCCGCCCCGTCCGTGGTCTATGATAGGGAGCAGTGCCTCCTACTGTAGGTCTGACTCACG
  ACCGATGAGGGGCATGGTGACATGTCCCTGTTCTGGTACCTCTGTGGCCCTGGATCATTC
  TATTTTGTCACCATATACCGATCTGTCCATGGCACTTTCACCCTTGTCTATAAAGGCTAA
  ATGTAGATGGTCCCGCCTCCTTTTCCCCTCTTTTTTTCGTGGTCACGACCGCCACTTCAT
  CGCGGAATTCCATTTCTGCCTATTTCTCCTGCGGAGACATCAATGATTTCTGTTTTTCTT
  TTTGCCCTGTGTACTAACCTTTCTTTCCCTCTTGGGCTCCTGTTTGTATCCTCCATAGAT
  AGTCTTTCCCTCCGCCACTTAATGATTTCCATTTCTTTCTCCCGGTACCTATATCTTCGC
  CCTGGTCCTTGTCATCACCCGGTTCCCATTTCTTTCTTGGTCCCCCATTTGTCCCGTGTT
  CTCATCCATCTCCCCCGAATACTCTCTCGTTTTCTTGTCAATGCCGGCCCGGTTATGGTT
  GGTTTGTCTCTTCCCAATAGCCTTTTTCCTTCAATTCTCCAACCCCTCCTGCTATTTATC
  TGGGTGGCGCTTCACCATCAATATGCTCCCCCCTCTTTTTCCGTGTTAGATCCCCCCCCT
  TCGCTTTCCCCCGTATTCTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_030655
• Insert Size: 4100 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_030655.2, NP_085913.1
• RefSeq Size: 4203 bp
• RefSeq ORF: 4203 bp
• Locus ID: 1663
• Cytogenetics: 12p11.21
• Domains: DEXDc2
• Protein Families: Stem cell - Pluripotency
• Gene Summary: 'DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is an enzyme that possesses both ATPase and DNA helicase activities. This gene is a homolog of the yeast CHL1 gene, and may function to maintain chromosome transmission fidelity and genome stability. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (3) contains exon 6, exon 21, and intron 27, as compared to variants 1 and 2. The presence of the exon 6 shifts the open reading frame and introduces an upstream stop codon within exon 7. As a result, this variant has a longer nucleotide sequence, but encodes a shorter isoform than variants 1 and 2.