MRPL27 (NM_016504) Human Untagged Clone

CAT#: SC127560

MRPL27 (untagged)-Human mitochondrial ribosomal protein L27 (MRPL27), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MRPL27
• Synonyms: L27mt
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC127560 sequence for NM_016504 edited (data generated by NextGen Sequencing)
  ATGGCGTCGGTGGTGTTGGCGCTGAGGACCCGGACAGCCGTTACATCCTTGCTAAGCCCC
  ACTCCGGCTACAGCTCTTGCTGTCAGATACGCATCCAAGAAGTCGGGTGGTAGCTCCAAA
  AACCTCGGTGGAAAGTCATCAGGCAGACGCCAAGGCATTAAGAAAATGGAAGGTCACTAT
  GTTCATGCTGGGAACATCATTGCAACACAGCGCCATTTCCGCTGGCACCCAGGTGCCCAT
  GTGGGTGTTGGGAAGAATAAATGTCTGTATGCCCTGGAAGAGGGGATAGTCCGCTACACT
  AAGGAGGTCTACGTGCCTCATCCCAGAAACACGGAGGCTGTGGATCTGATCACCAGGCTG
  CCCAAGGGTGCTGTGCTCTACAAGACTTTTGTCCACGTGGTTCCTGCCAAGCCTGAGGGC
  ACCTTCAAACTGGTAGCTATGCTTTGA
  
  Clone variation with respect to NM_016504.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_016504 unedited
  TGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGGAGTGATCGAAAGCATGGC
  GTCGGTGGTGTTGGCGCTGAGGACCCGGACAGCCGTTACATCCTTGCTAAGCCCCACTCC
  GGCTACAGCTCTTGCTGTCAGATACGCATCCAAGAAGTCGGGTGGTAGCTCCAAAAACCT
  CGGTGGAAAGTCATCAGGCAGACGCCAAGGCATTAAGAAAATGGAAGGTCACTATGTTCA
  TGCTGGGAACATCATTGCAACACAGCGCCATTTCCGCTGGCACCCAGGTGCCCATGTGGG
  TGTTGGGAAGAATAAATGTCTGTATGCCCTGGAAGAGGGGATAGTCCGCTACACTAAGGA
  GGTCTACGTGCCTCATCCCAGAAACACGGAGGCTGTGGATCTGATCACCAGGCTGCCCAA
  GGGTGCTGTGCTCTACAAGACTTTTGTCCACGTGGTTCCTGCCAAGCCTGAGGGCACCTT
  CAAACTGGTAGCTATGCTTTGATGTCCTGTTGAGGCCATCGGACAGAGACTGGAGCCCAG
  GTGACAGGAGATGGTGATACCAGAAGTCAAGGGTTGGGGTGGCGACACGGCCTCCCGAGG
  AAGAGGTCTGCTTGATGGTGACTCTGCAGGAGACTCTGAAGTGACTGCTGGGAAACCCTT
  TGGGAGACCTGACCTGNGGCCAAANATAAAGTGAGCCAGCGTCATGAACGCATGCTATTT
  ANGGACCAAAAAAAAAAAAAAACTCGACTCTAGATTGCGGGCCGCGTCATAGCTGTTTCC
  TGAACAGATCCCGGGTGGCATCCNTGTGACCCCTNCCCAGTGCCTCTCCTGGCCCTGGNA
  ATTGCCACTCCAGTGCCCACCAGCCTTGNCCTAATAAAATTAAGTGCATCATTTGGCTG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_016504
• ORF Size: 447 bp
• Insert Size: 2750
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_016504.2, NP_057588.1
• RefSeq Size: 728
• RefSeq ORF: 447
• Locus ID: 51264
• Domains: Ribosomal_L27
• Gene Summary: Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) encodes the longest isoform (a).