PSMD4 (NM_153822) Human Untagged Clone

CAT#: SC127580

PSMD4 (untagged)-Human proteasome (prosome, macropain) 26S subunit, non-ATPase, 4 (PSMD4), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PSMD4
• Synonyms: AF, ASF, S5A, AF-1, MCB1, Rpn10, pUB-R5
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_153822, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGTGTTGGAAAGCACTATGGTGTGTGTGGACAACAGTGAGTATATGCGGAATGGAGACTTCTTACCCA
  CCAGGCTGCAGGCCCAGCAGGATGCTGTCAACATAGTTTGTCATTCAAAGACCCGCAGCAACCCTGAGAA
  CAACGTGGGCCTTATCACACTGGCTAATGACTGTGAAGTGCTGACCACACTCACCCCAGACACTGGCCGT
  ATCCTGTCCAAGCTACATACTGTCCAACCCAAGGGCAAGATCACCTTCTGCACGGGCATCCGCGTGGCCC
  ATCTGGCTCTGAAGCACCGACAAGGCAAGAATCACAAGATGCGCATCATTGCCTTTGTGGGAAGCCCAGT
  GGAGGACAATGAGAAGGATCTGGTGAAACTGGCTAAACGCCTCAAGAAGGAGAAAGTAAATGTTGACATT
  ATCAATTTTGGGGAAGAGGAGGTGAACACAGAAAAGCTGACAGCCTTTGTAAACACGTTGAATGGCAAAG
  ATGGAACCGGTTCTCATCTGGTGACAGTGCCTCCTGGGCCCAGTTTGGCTGATGCTCTCATCAGTTCTCC
  GATTTTGGCTGGTGAAGGTGGTGCCATGCTGGGTCTTGGTGCCAGTGACTTTGAATTTGGAGTAGATCCC
  AGTGCTGATCCTGAGCTGGCCTTGGCCCTTCGTGTATCTATGGAAGAGCAGCGGCAGCGGCAGGAGGAGG
  AGGCCCGGCGGGCAGCTGCAGCTTCTGCTGCTGAGGCCGGGATTGCTACGACTGGGACTGAAGGTGAAAG
  AGGTGGAATCCGAAGTCCTGGGACTGCGGGATGCTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_153822 unedited
  AATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTCTCTCTCTCTCTCTCAC
  CCGGTCGGGAGGGAGGAAGGTGGCAAGATGGTGTTGGAAAGCACTATGGTGTGTGTGGAC
  AACAGTGAGTATATGCGGAATGGAGACTTCTTACCCACCAGGCTGCAGGCCCAGCAGGAT
  GCTGTCAACATAGTTTGTCATTCAAAGACCCGCAGCAACCCTGAGAACAACGTGGGCCTT
  ATCACACTGGCTAATGACTGTGAAGTGCTGACCACACTCACCCCAGACACTGGCCGTATC
  CTGTCCAAGCTACATACTGTCCAACCCAAGGGCAAGATCACCTTCTGCACGGGCATCCGC
  GTGGCCCATCTGGCTCTGAAGCACCGACAAGGCAAGAATCACAAGATGCGCATCATTGCC
  TTTGTGGGAAGCCCAGTGGAGGACAATGAGAAGGATCTGGTGAAACTGGCTAAACGCCTC
  AAGAAGGAGAAAGTAAATGTTGACATTATCAATTTTGGGGAAGAGGAGGTGAACACAGAA
  AAGCTGACAGCCTTTGTAAACACGTTGAATGGCAAAGATGGAACCGGTTCTCATCTGGTG
  ACAGTGCCTCCTGGGCCCAGTTTGGCTGATGCTCTCATCAGTTCTCCCGATTTTGCCTGG
  TGAAGGTGGTGCCATGCCTGGTCTTGCTGCCAGTGACCTTTGAATTTGGAGTAGATCCCA
  GTGCTGATCCTGAACTGGCCCTTGGCCCTTCTGGTTTCTATGAACACCANCGGCACCGGC
  AAGAAGAGGAGGCCCGGCTGCCAGTTGTACTTTTGCTGCTGAAGCCCGGACTGCTACCAC
  TGGGACTGAAGTGAAAAAGCACCACACAATGCCCTGCTGAAGATGACCATCACCCCCAAG
  ATTTGCGCGCCTGGGCTTTCTGACCAAACAAT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_153822 unedited
  GGCAGCTATACGCAGTTTGTAATGGTGACAGCAAACACACATNCTAACCCTATATTCCGT
  GTGTCCCATGCGTCCCCTAAGCAGACTGAGCTACCCTTTCCCTCCAGTCTCACTTCTTGT
  CTTCCTCCTTCTTGTCCTTCTTGCCGTCCTTGGTGGCCTGGGAGGCCAGGGAGCCCATAC
  CATTTCGAATGGCTTCATTGTTGGGATCCACACCTGGGAGGTCCTCTAGGACACTCTGAG
  GGAGACTCGGGGTCCTGCATCACGTCGTAATCATCCTCCTCCTTGGCTGGCTCGGATGTG
  TCCATAGCTGAGCTGGCATCAATGTCTGCTGATTCCGACTGGCCAAACTCTGCTCCCTGC
  AGGGACATCTGCATGGCATAAGCAATCTGCTCTTCCTCAGCCATACTGCTTAGGTCAGGA
  AGCCCAGTGCGGCCAAACTCTTGCTGGCTGATGGTCATCTTCAGCAGGGCATCGGCTGAG
  TCTCTTTCACCTTCATTCCCAGTCGTATCAATACCGGCCTCACCACCATAAGCTGTACCT
  GCCCGCCGGGCCTACTCCTACTGCCGNTGACGGTGCTCTTTCATACATACACGAATGGCC
  AACGCCAGCTCAGGATCAGCACTGAGTATCTACTCCAAATTCAAAGTCACTGGCACCAAG
  ACCCACCATGGCCACCACCTTCACCACGCGAATTCGGAGAACTGATGATAGCATCAACCA
  GCACGGGCCCACGAGGTACTTTCACAGATGAGATCCGGGTCCCATCTTGCCTTTAACCTT
  TTACCAAGGCTCTCAGCTTCTCCTGGTTAACCTCCCTTTCCCCAATGGGAAAGGCAACCT
  CACTATCCCCTCTTCAGCGTATACCGTCGTCGACACCTGTCCATGTCCCCCTCGGCTCCC
  CCATGCCCGAGACCTCCTGGCAGTTGCCTTGCGCCCTCCACCCAGGGCCCTCGATCCGTG
  CTAAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_153822
• Insert Size: 1500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_153822.1, NP_722544.1
• RefSeq Size: 1527 bp
• RefSeq ORF: 1527 bp
• Locus ID: 5710
• Cytogenetics: 1q21.3
• Protein Pathways: Proteasome
• Gene Summary: 'The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the non-ATPase subunits of the 19S regulator lid. Pseudogenes have been identified on chromosomes 10 and 21. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (2) includes an alternate segment, compared to variant 1, that causes a frameshift. The resulting protein (isoform 2) has a distinct C-terminus compared to isoform 1.