ATP5F1B (NM_001686) Human Untagged Clone

CAT#: SC127589

ATP5B (untagged)-Human ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide (ATP5B), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ATP5F1B
• Synonyms: ATP5B; ATPMB; ATPSB; HEL-S-271
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001686, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTTGGGGTTTGTGGGTCGGGTGGCCGCTGCTCCGGCCTCCGGGGCCTTGCGGAGACTCACCCCTTCAG
  CGTCGCTGCCCCCAGCTCAGCTCTTACTGCGGGCCGCTCCGACGGCGGTCCATCCTGTCAGGGACTATGC
  GGCGCAAACATCTCCTTCGCCAAAAGCAGGCGCCGCCACCGGGCGCATCGTGGCGGTCATTGGCGCAGTG
  GTGGACGTCCAGTTTGATGAGGGACTACCACCAATTCTAAATGCCCTGGAAGTGCAAGGCAGGGAGACCA
  GACTGGTTTTGGAGGTGGCCCAGCATTTGGGTGAGAGCACAGTAAGGACTATTGCTATGGATGGTACAGA
  AGGCTTGGTTAGAGGCCAGAAAGTACTGGATTCTGGTGCACCAATCAAAATTCCTGTTGGTCCTGAGACT
  TTGGGCAGAATCATGAATGTCATTGGAGAACCTATTGATGAAAGAGGTCCCATCAAAACCAAACAATTTG
  CTCCCATTCATGCTGAGGCTCCAGAGTTCATGGAAATGAGTGTTGAGCAGGAAATTCTGGTGACTGGTAT
  CAAGGTTGTCGATCTGCTAGCTCCCTATGCCAAGGGTGGCAAAATTGGGCTTTTTGGTGGTGCTGGAGTT
  GGCAAGACTGTACTGATCATGGAGTTAATCAACAATGTCGCCAAAGCCCATGGTGGTTACTCTGTGTTTG
  CTGGTGTTGGTGAGAGGACCCGTGAAGGCAATGATTTATACCATGAAATGATTGAATCTGGTGTTATCAA
  CTTAAAAGATGCCACCTCTAAGGTAGCGCTGGTATATGGTCAAATGAATGAACCACCTGGTGCTCGTGCC
  CGGGTAGCTCTGACTGGGCTGACTGTGGCTGAATACTTCAGAGACCAAGAAGGTCAAGATGTACTGCTAT
  TTATTGATAACATCTTTCGCTTCACCCAGGCTGGTTCAGAGGTGTCTGCATTATTGGGCCGAATCCCTTC
  TGCTGTGGGCTATCAGCCTACCCTGGCCACTGACATGGGTACTATGCAGGAAAGAATTACCACTACCAAG
  AAGGGATCTATCACCTCTGTACAGGCTATCTATGTGCCTGCTGATGACTTGACTGACCCTGCCCCTGCTA
  CTACGTTTGCCCATTTGGATGCTACCACTGTACTGTCGCGTGCCATTGCTGAGCTGGGCATCTATCCAGC
  TGTGGATCCTCTAGACTCCACCTCTCGTATCATGGATCCCAACATTGTTGGCAGTGAGCATTACGATGTT
  GCCCGTGGGGTGCAAAAGATCCTGCAGGACTACAAATCCCTCCAGGATATCATTGCCATCCTGGGTATGG
  ATGAACTTTCTGAGGAAGACAAGTTGACCGTGTCCCGTGCACGGAAAATACAGCGTTTCTTGTCTCAGCC
  ATTCCAGGTTGCTGAGGTCTTCACAGGTCATATGGGGAAGCTGGTACCCCTGAAGGAGACCATCAAAGGA
  TTCCAGCAGATTTTGGCAGGTGAATATGACCATCTCCCAGAACAGGCCTTCTATATGGTGGGACCCATTG
  AAGAAGCTGTGGCAAAAGCTGATAAGCTGGCTGAAGAGCATTCATCGTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001686 unedited
  CGCGAATTCGGCACCAGCTACGCCATGTTGGGGTTTGTGGGTCGGGTGGCCGCTGCTCCG
  GCCTCCGGGGCCTTGCGGAGACTCACCCCTTCAGCGTCGCTGCCCCCAGCTCAGCTCTTA
  CTGCGGGCCGCTCCGACGGCGGTCCATCCTGTCAGGGACTATGCGGCGCAAACATCTCCT
  TCGCCAAAAGCAGGCGCCGCCACCGGGCGCATCGTGGCGGTCATTGGCGCAGTGGTGGAC
  GTCCAGTTTGATGAGGGACTACCACCAATTCTAAATGCCCTGGAAGTGCAAGGCAGGGAG
  ACCAGACTGGTTTTGGAGGTGGCCCAGCATTTGGGTGAGAGCACAGTAAGGACTATTGCT
  ATGGATGGTACAGAAGGCTTGGTTAGAGGCCAGAAAGTACTGGATTCTGGTGCACCAATC
  AAAATTCCTGTTGGTCCTGAGACTTTGGGCAGAATCATGAATGTCATTGGAGAACCTATT
  GATGAAAGAGGTCCCATCAAAACCAAACAATTTGCTCCCATTCATGCTGAGGCTCCAGAG
  TTCATGGAAATGAGTGTTGAGCAGGAAATTCTGGTGACTGGTATCAAGGTTGTCGATCTG
  CTAGCTCCCTATGCCAAGGGTGGNCAAATTGGGCTNTTTGGTGGTGCTGGAGTTGGCAAG
  ACTGTACTGATCATGGAGTTAATCAAACATGTCGCCAAAGCCCATGGTGGTTACTCTGTG
  TTTGCTGGTNGTNGGTGAAAGGACCCGTGAN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001686 unedited
  ATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGAGGGGTGTACATT
  TTATTGGAAACCTTAAATACTGTTCAGAAAGAATATATCTTCAATCAAGGCTCTTGTGCA
  GCCTACACAGAAAAATGAAGCTTTTTGGGTTAGGGGCAAGGAGAGAGACAGTACAGAGGA
  CAAAGACCCCTCACGATGAATGCTCTTCAGCCAGCTTATCAGCTTTTGCCACAGCTTCTT
  CAATGGGTCCCACCATATAGAAGGCCTGTTCTGGGAGATGGTCATATTCACCTGCCAAAA
  TCTGCTGGAATCCTTTGATGGTCTCCTTCAGGGGTACCAGCTTCCCCATATGACCTGTGA
  AGACCTCAGCAACCTGGAATGGCTGAGACAAGAAACGCTGTATTTTCCGTGCACGGGACA
  CGGTCAACTTGTCTTCCTCAGAAAGTTCATCCATACCCAGGATGGCAATGATATCCTGGA
  GGGATTTGTAGTCCTGCAGGATCTTTTGCACCCCACGGGCAACATCGTAATGCTCACTGC
  CAACAATGTTGGGATCCATGATACGAGAGGTGGAGTCTAGAGGATCCACAGCTGGATAGA
  TGCCCAGCTCAGCAATGGCACGCGACAGTACAGTGGTAGCATCCAAATGGGCAAACGTAG
  TAGCAGGGGCAGGGTCAGTCAAGTCATCAGCAGGCACATAGATAGCCTGTACAGAGGTGA
  TAGATCCCTTCTTGGTAGTGGTAATTCTTTCCTGCATAGTCCCCATGTCAGTGGCCAGGG
  TAAGCTGATACCCCCCACATAAGGAATTCGGCCCATAATGCANACACCTCTGAACCAGCC
  TGGGTGAACCGCAGCAGCCTTTATTTAACCCCCCCCTTCTCGACCCTTTTGGTCTTGAAG
  TTTTCACCCCATCAGCCCATACAGATTACCGGCAACAGCCCAGGGGTTATTAATTGACCC
  CACAACGCTCCCTCAAGGGCATTTCTAAGTGAAACCCA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001686
• Insert Size: 1750 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001686.3, NP_001677.2
• RefSeq Size: 1857 bp
• RefSeq ORF: 1590 bp
• Locus ID: 506
• Cytogenetics: 12q13.3
• Domains: ATP-synt_ab, ATP-synt_ab_C, AAA, ATP-synt_ab_N
• Protein Families: Druggable Genome
• Protein Pathways: Alzheimer's disease, Huntington's disease, Metabolic pathways, Oxidative phosphorylation, Parkinson's disease
• Gene Summary: 'This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the beta subunit of the catalytic core. [provided by RefSeq, Jul 2008]'