STT3A (NM_152713) Human Untagged Clone

CAT#: SC127610

STT3A (untagged)-Human STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae) (STT3A)


  "NM_152713" in other vectors (6)

Reconstitution Protocol

USD 310.00

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol STT3A
Synonyms ITM1; STT3-A; TMC
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC127610 sequence for NM_152713 edited (data generated by NextGen Sequencing)
ATGACTAAGTTTGGATTTTTGCGATTGTCCTATGAGAAGCAGGACACACTTTTGAAGCTT
CTCATTCTGTCAATGGCTGCTGTATTATCCTTCTCCACTCGTCTGTTTGCTGTCCTGAGA
TTTGAAAGTGTTATCCATGAGTTTGATCCGTACTTTAATTATCGGACTACCAGGTTCCTG
GCTGAGGAGGGGTTTTATAAATTCCATAACTGGTTTGATGACCGAGCCTGGTACCCTTTG
GGACGAATCATTGGAGGAACAATTTACCCAGGTTTAATGATCACCTCTGCTGCAATCTAC
CATGTACTCCATTTTTTCCACATCACCATCGACATTCGGAATGTCTGTGTGTTCCTGGCC
CCTCTCTTCTCCTCCTTCACCACCATCGTCACGTACCACCTTACCAAAGAGCTCAAGGAT
GCAGGGGCTGGGCTTCTTGCTGCTGCCATGATTGCTGTAGTTCCTGGATATATCTCCCGA
TCTGTGGCTGGCTCCTATGATAATGAAGGGATTGCCATCTTTTGCATGCTACTCACCTAC
TACATGTGGATCAAGGCAGTAAAGACTGGTTCCATCTGTTGGGCAGCTAAGTGTGCCCTT
GCTTATTTCTACATGGTCTCGTCATGGGGAGGTTATGTGTTCCTGATCAACTTAATTCCT
CTCCACGTCCTCGTGCTGATGCTCACAGGCCGTTTCTCTCACCGGATCTATGTGGCCTAC
TGTACTGTTTACTGCCTGGGCACTATACTTTCTATGCAGATCTCCTTTGTGGGTTTCCAG
CCTGTCCTTTCATCAGAGCACATGGCAGCCTTTGGGGTCTTTGGTCTCTGCCAGATCCAT
GCCTTTGTGGATTACCTGCGCAGCAAGTTGAATCCACAACAATTTGAAGTTCTTTTCCGG
AGCGTCATCTCTCTGGTAGGCTTTGTCCTTCTCACCGTGGGAGCTCTCCTCATGCTGACA
GGAAAAATATCTCCCTGGACGGGGCGTTTCTACTCGCTGCTGGATCCCTCTTATGCTAAG
AACAACATCCCCATCATTGCTTCTGTGTCTGAGCATCAGCCCACAACCTGGTCCTCATAC
TATTTTGACCTGCAGCTCCTCGTCTTCATGTTTCCAGTTGGCCTCTATTACTGCTTTAGC
AACCTGTCTGATGCCCGGATTTTTATCATCATGTATGGTGTGACCAGCATGTACTTTTCA
GCTGTAATGGTGCGTCTAATGCTAGTGTTGGCACCTGTTATGTGCATTCTCTCTGGCATT
GGAGTCTCCCAGGTGCTGTCCACATACATGAAGAATCTGGACATAAGTCGTCCAGACAAG
AAGAGCAAGAAGCAACAGGATTCCACCTACCCTATTAAGAATGAAGTGGCAAGTGGGATG
ATACTGGTCATGGCTTTCTTTCTCATCACCTACACCTTTCATTCAACCTGGGTGACCAGT
GAGGCCTACTCTTCTCCGTCCATTGTACTATCTGCCCGTGGTGGGGATGGCAGTAGGATC
ATATTTGATGACTTCCGAGAAGCATATTATTGGCTTCGTCATAATACTCCAGAGGATGCG
AAGGTCATGTCCTGGTGGGATTATGGCTATCAGATTACAGCTATGGCAAACCGAACAATT
TTAGTGGACAATAACACATGGAATAATACCCATATTTCTCGAGTAGGGCAGGCAATGGCG
TCCACAGAGGAAAAAGCCTATGAGATCATGAGGGAGCTCGATGTCAGCTATGTGCTGGTC
ATTTTTGGAGGCCTCACTGGGTATTCCTCTGATGATATCAACAAGTTTCTTTGGATGGTC
CGGATTGGAGGGAGCACAGATACAGGCAAACATATCAAGGAGAATGACTATTATACTCCA
ACTGGGGAGTTCCGTGTGGACCGTGAAGGTTCTCCAGTGCTGCTCAACTGCCTCATGTAC
AAGATGTGTTACTATCGCTTTGGACAGGTTTACACAGAAGCCAAGCGTCCTCCAGGCTTT
GACCGTGTCCGAAATGCTGAGATTGGGAATAAAGACTTTGAGCTTGATGTCCTGGAGGAA
GCATATACCACAGAACATTGGCTGGTCAGGATATACAAGGTAAAGGACCTGGATAATCGA
GGCTTGTCAAGGACATAA

Clone variation with respect to NM_152713.3
>OriGene 5' read for NM_152713 unedited
GATTTTGTAATACGACTCACTTATAGGGCGGCCGCGATTCGGCACGAGGGATGGCTGAGG
GAGCCCCGCGGATCGTTTAGGAAAGCCGGCCAGCTGATCGTCGTGTGTTGCCACCCATTC
ATGTCAAGATGACTAAGTTTGGATTTTTGCGATTGTCCTATGAGAAGCAGGACACACTTT
TGAAGCTTCTCATTCTGTCAATGGCTGCTGTATTATCCTTCTCCACTCGTCTGTTTGCTG
TCCTGAGATTTGAAAGTGTTATCCATGAGTTTGATCCGTACTTTAATTATCGGACTACCA
GGTTCCTGGCTGAGGAGGGGTTTTATAAATTCCATAACTGGTTTGATGACCGAGCCTGGT
ACCCTTTGGGACGAATCATTGGAGGAACAATTTACCCAGGTTTAATGATCACCTCTGCTG
CAATCTACCATGTACTCCATTTTTTCCACATCACCATCGACATTCGGAATGTCTGTGTGT
TCCTGGCCCCTCTCTTCTCCTCCTTCACCACCATCGTCACGTACCACCTTACCAAAGAGC
TCAAGGATGCNAGGGGCTGGGCTTCTTGCTGCTGCCATGATTGCTTGTAGTTCCTGGATA
TATCTCCCGATCTGGGGCTGGCTCCTATGATAATGAAAGGGATTGCCATCTTTTGCATGC
TACTCACCTACTACATGGTGGATCAAGGCAGTAAGGACTGGTTCCATTCTGTTGGCAGCC
TAAGTGTGCCCTTGCTTATTCTACATGGTCCGCTTGGGGGGGGGATTGTGGGTCCTGAAA
ACTAATTCTCTCCCGTCCTCGGGCGAGCTACCGGGCCGTTCTTCCCGATTCTATTGGCCA
CGGACTGGTTACTGCTGGCACTATCTTTAATCAATTCTTTTTGGGGGTCCCGCCCGCCTT
CT
>OriGene 3' read for NM_152713 unedited
GGGCCGCAATCTAAAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
TTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAAAAATCCCCCCCTTTATTTGCCCAAAGAA
AACCCCTGACTTTTTTTTGGGGAACCGGTTAAAAATTAAAAAAATTTTTTTTTTGGGCCC
ACCCCTTTCTGCCCAAAAAAAAATTTCCAAACAATTTTGAGGCCCTCCAGTTTTGTTTTT
ACAAACGGGTTTTTAAAAAAAAAAAAAAAAAAAATTTTTCAACGCCCTAAAGGGGAGGGG
CTCAGGGGAAAACTTTTAAAAGGGGGGCGGGACTTTTTTGTCCTTGACAACCCTAAATTT
TCCAGGCCCTTTACCTTGTTTTTCCGGACCACCCAAGGTTTGGGGGTATATGCTTCCTCC
AAAACATAAAGCTAAAAGTTTTTTTTCCCAATTTAAAATTTTGGGACCCGGTAAAACCCT
GGAGGACCCTTGGTTTTTGGGAAAACCTGCCCAAAGGGATAGTAACCCATTTTGTCCATG
GGGGAGTTGACCACCCCTGGAAAACCTTCCGGGCCCACACGGAACTCCCCAGGGGGAGTA
AAAAAGCCCTTCTCCTTGATATGTTGGCCTGTATTTGGGCCCCCTCCAATCCGGACCATC
CAAAAAAACTTGTTGATTTCTTCAGAGGATTCCCCGGTGAGGGCCCCAAAAATGACCAGC
CAATAGCTGGAATCNGGGCTCCCTCAAGAACCAATAGGCTTTTCCCTCTGGGACCCCTTT
GCCGGCCCCACTGAAAAAATAGGGGTTTATCCATGGGGTTTTGCCCACTAAATGGCGGGT
TGCCTTCCTGGATTTGAAGCCTTATCCCACCAGAATGACCTCTCATCTTGGGAGTTATGA
CAAACCAATATAGCTTTGGAAGCATCAATGACCTCGGCCTCCCCCACGCAATATACTACG
GAAAAA
Restriction Sites NotI-NotI     
ACCN NM_152713
Insert Size 2560 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_152713.2, NP_689926.1
RefSeq Size 2516 bp
RefSeq ORF 2118 bp
Locus ID 3703
Cytogenetics 11q24.2
Domains STT3
Protein Families Transmembrane
Protein Pathways Metabolic pathways, N-Glycan biosynthesis
Gene Summary 'The protein encoded by this gene is a catalytic subunit of the N-oligosaccharyltransferase (OST) complex, which functions in the endoplasmic reticulum to transfer glycan chains to asparagine residues of target proteins. A separate complex containing a similar catalytic subunit with an overlapping function also exists. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2015]'
Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 both encode the same isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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