STT3A (NM_152713) Human Untagged Clone

CAT#: SC127610

STT3A (untagged)-Human STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae) (STT3A)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: STT3A
• Synonyms: ITM1; STT3-A; TMC
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC127610 sequence for NM_152713 edited (data generated by NextGen Sequencing)
  ATGACTAAGTTTGGATTTTTGCGATTGTCCTATGAGAAGCAGGACACACTTTTGAAGCTT
  CTCATTCTGTCAATGGCTGCTGTATTATCCTTCTCCACTCGTCTGTTTGCTGTCCTGAGA
  TTTGAAAGTGTTATCCATGAGTTTGATCCGTACTTTAATTATCGGACTACCAGGTTCCTG
  GCTGAGGAGGGGTTTTATAAATTCCATAACTGGTTTGATGACCGAGCCTGGTACCCTTTG
  GGACGAATCATTGGAGGAACAATTTACCCAGGTTTAATGATCACCTCTGCTGCAATCTAC
  CATGTACTCCATTTTTTCCACATCACCATCGACATTCGGAATGTCTGTGTGTTCCTGGCC
  CCTCTCTTCTCCTCCTTCACCACCATCGTCACGTACCACCTTACCAAAGAGCTCAAGGAT
  GCAGGGGCTGGGCTTCTTGCTGCTGCCATGATTGCTGTAGTTCCTGGATATATCTCCCGA
  TCTGTGGCTGGCTCCTATGATAATGAAGGGATTGCCATCTTTTGCATGCTACTCACCTAC
  TACATGTGGATCAAGGCAGTAAAGACTGGTTCCATCTGTTGGGCAGCTAAGTGTGCCCTT
  GCTTATTTCTACATGGTCTCGTCATGGGGAGGTTATGTGTTCCTGATCAACTTAATTCCT
  CTCCACGTCCTCGTGCTGATGCTCACAGGCCGTTTCTCTCACCGGATCTATGTGGCCTAC
  TGTACTGTTTACTGCCTGGGCACTATACTTTCTATGCAGATCTCCTTTGTGGGTTTCCAG
  CCTGTCCTTTCATCAGAGCACATGGCAGCCTTTGGGGTCTTTGGTCTCTGCCAGATCCAT
  GCCTTTGTGGATTACCTGCGCAGCAAGTTGAATCCACAACAATTTGAAGTTCTTTTCCGG
  AGCGTCATCTCTCTGGTAGGCTTTGTCCTTCTCACCGTGGGAGCTCTCCTCATGCTGACA
  GGAAAAATATCTCCCTGGACGGGGCGTTTCTACTCGCTGCTGGATCCCTCTTATGCTAAG
  AACAACATCCCCATCATTGCTTCTGTGTCTGAGCATCAGCCCACAACCTGGTCCTCATAC
  TATTTTGACCTGCAGCTCCTCGTCTTCATGTTTCCAGTTGGCCTCTATTACTGCTTTAGC
  AACCTGTCTGATGCCCGGATTTTTATCATCATGTATGGTGTGACCAGCATGTACTTTTCA
  GCTGTAATGGTGCGTCTAATGCTAGTGTTGGCACCTGTTATGTGCATTCTCTCTGGCATT
  GGAGTCTCCCAGGTGCTGTCCACATACATGAAGAATCTGGACATAAGTCGTCCAGACAAG
  AAGAGCAAGAAGCAACAGGATTCCACCTACCCTATTAAGAATGAAGTGGCAAGTGGGATG
  ATACTGGTCATGGCTTTCTTTCTCATCACCTACACCTTTCATTCAACCTGGGTGACCAGT
  GAGGCCTACTCTTCTCCGTCCATTGTACTATCTGCCCGTGGTGGGGATGGCAGTAGGATC
  ATATTTGATGACTTCCGAGAAGCATATTATTGGCTTCGTCATAATACTCCAGAGGATGCG
  AAGGTCATGTCCTGGTGGGATTATGGCTATCAGATTACAGCTATGGCAAACCGAACAATT
  TTAGTGGACAATAACACATGGAATAATACCCATATTTCTCGAGTAGGGCAGGCAATGGCG
  TCCACAGAGGAAAAAGCCTATGAGATCATGAGGGAGCTCGATGTCAGCTATGTGCTGGTC
  ATTTTTGGAGGCCTCACTGGGTATTCCTCTGATGATATCAACAAGTTTCTTTGGATGGTC
  CGGATTGGAGGGAGCACAGATACAGGCAAACATATCAAGGAGAATGACTATTATACTCCA
  ACTGGGGAGTTCCGTGTGGACCGTGAAGGTTCTCCAGTGCTGCTCAACTGCCTCATGTAC
  AAGATGTGTTACTATCGCTTTGGACAGGTTTACACAGAAGCCAAGCGTCCTCCAGGCTTT
  GACCGTGTCCGAAATGCTGAGATTGGGAATAAAGACTTTGAGCTTGATGTCCTGGAGGAA
  GCATATACCACAGAACATTGGCTGGTCAGGATATACAAGGTAAAGGACCTGGATAATCGA
  GGCTTGTCAAGGACATAA
  
  Clone variation with respect to NM_152713.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_152713 unedited
  GATTTTGTAATACGACTCACTTATAGGGCGGCCGCGATTCGGCACGAGGGATGGCTGAGG
  GAGCCCCGCGGATCGTTTAGGAAAGCCGGCCAGCTGATCGTCGTGTGTTGCCACCCATTC
  ATGTCAAGATGACTAAGTTTGGATTTTTGCGATTGTCCTATGAGAAGCAGGACACACTTT
  TGAAGCTTCTCATTCTGTCAATGGCTGCTGTATTATCCTTCTCCACTCGTCTGTTTGCTG
  TCCTGAGATTTGAAAGTGTTATCCATGAGTTTGATCCGTACTTTAATTATCGGACTACCA
  GGTTCCTGGCTGAGGAGGGGTTTTATAAATTCCATAACTGGTTTGATGACCGAGCCTGGT
  ACCCTTTGGGACGAATCATTGGAGGAACAATTTACCCAGGTTTAATGATCACCTCTGCTG
  CAATCTACCATGTACTCCATTTTTTCCACATCACCATCGACATTCGGAATGTCTGTGTGT
  TCCTGGCCCCTCTCTTCTCCTCCTTCACCACCATCGTCACGTACCACCTTACCAAAGAGC
  TCAAGGATGCNAGGGGCTGGGCTTCTTGCTGCTGCCATGATTGCTTGTAGTTCCTGGATA
  TATCTCCCGATCTGGGGCTGGCTCCTATGATAATGAAAGGGATTGCCATCTTTTGCATGC
  TACTCACCTACTACATGGTGGATCAAGGCAGTAAGGACTGGTTCCATTCTGTTGGCAGCC
  TAAGTGTGCCCTTGCTTATTCTACATGGTCCGCTTGGGGGGGGGATTGTGGGTCCTGAAA
  ACTAATTCTCTCCCGTCCTCGGGCGAGCTACCGGGCCGTTCTTCCCGATTCTATTGGCCA
  CGGACTGGTTACTGCTGGCACTATCTTTAATCAATTCTTTTTGGGGGTCCCGCCCGCCTT
  CT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_152713 unedited
  GGGCCGCAATCTAAAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAAAAATCCCCCCCTTTATTTGCCCAAAGAA
  AACCCCTGACTTTTTTTTGGGGAACCGGTTAAAAATTAAAAAAATTTTTTTTTTGGGCCC
  ACCCCTTTCTGCCCAAAAAAAAATTTCCAAACAATTTTGAGGCCCTCCAGTTTTGTTTTT
  ACAAACGGGTTTTTAAAAAAAAAAAAAAAAAAAATTTTTCAACGCCCTAAAGGGGAGGGG
  CTCAGGGGAAAACTTTTAAAAGGGGGGCGGGACTTTTTTGTCCTTGACAACCCTAAATTT
  TCCAGGCCCTTTACCTTGTTTTTCCGGACCACCCAAGGTTTGGGGGTATATGCTTCCTCC
  AAAACATAAAGCTAAAAGTTTTTTTTCCCAATTTAAAATTTTGGGACCCGGTAAAACCCT
  GGAGGACCCTTGGTTTTTGGGAAAACCTGCCCAAAGGGATAGTAACCCATTTTGTCCATG
  GGGGAGTTGACCACCCCTGGAAAACCTTCCGGGCCCACACGGAACTCCCCAGGGGGAGTA
  AAAAAGCCCTTCTCCTTGATATGTTGGCCTGTATTTGGGCCCCCTCCAATCCGGACCATC
  CAAAAAAACTTGTTGATTTCTTCAGAGGATTCCCCGGTGAGGGCCCCAAAAATGACCAGC
  CAATAGCTGGAATCNGGGCTCCCTCAAGAACCAATAGGCTTTTCCCTCTGGGACCCCTTT
  GCCGGCCCCACTGAAAAAATAGGGGTTTATCCATGGGGTTTTGCCCACTAAATGGCGGGT
  TGCCTTCCTGGATTTGAAGCCTTATCCCACCAGAATGACCTCTCATCTTGGGAGTTATGA
  CAAACCAATATAGCTTTGGAAGCATCAATGACCTCGGCCTCCCCCACGCAATATACTACG
  GAAAAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_152713
• Insert Size: 2560 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_152713.2, NP_689926.1
• RefSeq Size: 2516 bp
• RefSeq ORF: 2118 bp
• Locus ID: 3703
• Cytogenetics: 11q24.2
• Domains: STT3
• Protein Families: Transmembrane
• Protein Pathways: Metabolic pathways, N-Glycan biosynthesis
• Gene Summary: 'The protein encoded by this gene is a catalytic subunit of the N-oligosaccharyltransferase (OST) complex, which functions in the endoplasmic reticulum to transfer glycan chains to asparagine residues of target proteins. A separate complex containing a similar catalytic subunit with an overlapping function also exists. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2015]'
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 both encode the same isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.