VAMP1 (NM_199245) Human Untagged Clone

CAT#: SC127642

VAMP1 (untagged)-Human vesicle-associated membrane protein 1 (synaptobrevin 1) (VAMP1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: VAMP1
• Synonyms: CMS25; SPAX1; SYB1; VAMP-1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC127642 sequence for NM_199245 edited (data generated by NextGen Sequencing)
  ATGTCTGCTCCAGCTCAGCCACCTGCTGAAGGGACAGAAGGGACTGCCCCAGGTGGGGGT
  CCCCCTGGCCCTCCTCCTAACATGACCAGTAACAGACGACTACAGCAAACCCAGGCACAA
  GTGGAGGAGGTGGTGGACATCATACGTGTGAACGTGGACAAGGTCCTGGAGAGGGACCAG
  AAGCTGTCAGAGCTGGATGACCGAGCTGATGCCTTGCAGGCAGGAGCATCACAATTTGAG
  AGCAGTGCTGCAAAGCTAAAGAGGAAGTATTGGTGGAAAAACTGCAAGATGATGATCATG
  CTGGGAGCCATCTGTGCCATCATCGTGGTAGTTATTGTAAGTAAGTATCGCTGA
  
  Clone variation with respect to NM_199245.1
  252 c=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_199245 unedited
  NTTGTCAAAATTTGTATACGACTCACTATAGGCGGCCGCGATTCGGCACGAGGCACTGAA
  GGATCCTCACAGCAACCGCTCCTTTCCGGAGTCGGATGAGAGGAGAGTTGTGACTGGCAA
  TTGGCAGGGGCGGGGCGGGCTAGGCCTGTAGCGCTGGGCGACCGTCCTGGGCATGGATTG
  GGCCGCGGGGTTGTCACCGTTATCCGGGAGGCGTGGTCAGCACTAATAAAGGCGGAGGCC
  GGCGCGGCAGCTGCAGTAAGTTCCAGCGCAGCTAGACCGCGGGGTAGTCGGCGCGAGGCG
  GAGCTTGGCAGTTCCGTCCACTTCAGCCGCAGCGTCCCTCGCCGGGTGTCTCGCCGCAGC
  CTCCGGAGAGGAACAGACCCTCACTCTCTCTGTCAGAAAAATGTCTGCTCCAGCTCAGCC
  ACCTGCTGAAGGGACAGAAGGGACTGCCCCAGGTGGGGGTCCCCCTGGCCCTCCTCCTAA
  CATGACCAGTAACAGACGACTACAGCAAACCCAGGCACAAGTGGAGGAGGTGGTGGACAT
  CATACGTGTGAACGTGGACAAGGTCCTGGAGAGGGACCAGAAGCTGTCAGAGCTGGATGA
  CCGAGCTGATGCCTTGCAGGCAGGAGCATCACAATTTGAGAGCAGTGCTGCAAAGCTAAA
  GAGGAAGTATTGGTGGAAAAACTGCAAGATGATGATCATGCTGGGAGCCATCTGTGCCTC
  ATCGTGGTAGTTATTGTAAGTAAGTATCGCTGAAGTTGCTGGTGGNGTGAAAGTGGGGAA
  AAGGTCCCTGAAGTCTTCTCCCTGCCTGCCTGCCTGCTGGNGGGAAGTGGGGGCTGCTCT
  GNACTGAGTATGGAGACCGTGTATGGGACGNCACCTGTTTNNNGGACCGGGGGGAAGGCA
  NAACAAGGGATTCTTTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_199245 unedited
  GGACGCGGCCGCAATCTAGTGTCGAGTTTTTTTTTTTTTTTTTTTTAATGGTAGGAATTT
  GTATTTTTTGCCTTTGTTCAGATACATGACATTGGTAAATATGCCACATGCCTTTGGTGG
  AAGTACAACTGTTGTTATTACTCTATACAAGTATGAGATCAGGGTTAGGAAAAAAAGACA
  AAGAGGTGATGACAGACACACAGTGGAAACCCCACATCGTCTCATGGCAAACCGAAGAAC
  GGGATGTGGGAAGCTCAGCTTCATTTGACTGCAAAGTCCCAGGGTTTTGTTGCACATTTG
  CTCATGCACATGGGTGGTGGGAGGAAAGGGGGATAGCAAAGACACACAGAAGAGGGTACA
  GGGTGGGTGAGAAAGAAAGTAGAAGGGCTAATACCCCCAAAGAACAAGGCCAACTACACC
  TGGTGAGCCTCAGAGGGACAGAAACCCAGGAATGATTCCTGTGATAGGGCTGGGAGAGCC
  AAGAGGACGGATTCGCTCCAGGCTTGGGACACATCGAGGACAGTGGTGGTTCTTCTCCAG
  CGGTGACCCCCTGCATTAGGCAAGGAGGAGCCCAGAGGAGAGTGGAGACCTTCGAGGGGG
  GCCGTTGGGAGGGTACTGACTGCTTTCTTCCAGCTCTTCAGTCCCGCCCTTGGGCAGGAC
  GAAGGGAATGTGGGAAACAAGGGCGAAGGGAAAGGAATGGATGGTTTTGAGCAATGAAAT
  GCTGCTGCATGGAAAGTGGGCATCCAGACCCTGCCCAGCATGGCCTCAGCCTCTTTCTGT
  TCGTGGACCGAGGGGAAGATGGATTAAAGGGCCATGGGCATTCTNCGCTCTGCTCCAGNC
  TGCCCTTCTCCCCTGCACCTGGGCTATCNCACATAATAGCCATNCTGAGCTCACAATGCC
  CCGAGATAGCTGAGCAGTCCATTCTCAGGTTCACTGTCTTACCACATCTGCNAAGGGGGG
  ATAAAGGGGCAAGTNAATCAACGGAAAGGGGGTCAAGGGAAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_199245
• Insert Size: 3420 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_199245.1, NP_954740.1
• RefSeq Size: 3151 bp
• RefSeq ORF: 354 bp
• Locus ID: 6843
• Cytogenetics: 12p13.31
• Protein Families: Secreted Protein, Transmembrane
• Protein Pathways: SNARE interactions in vesicular transport
• Gene Summary: 'Synapotobrevins, syntaxins, and the synaptosomal-associated protein SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. The protein encoded by this gene is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. Mutations in this gene are associated with autosomal dominant spastic ataxia 1. Multiple alternative splice variants have been described, but the full-length nature of some variants has not been defined. [provided by RefSeq, Jul 2014]'
  Transcript Variant: This variant (2), also known as VAMP-1D, has a different 3' structure, resulting in a novel 3' coding region and 3' UTR compared to variant 1. The encoded isoform (2) has a distinct C-terminus and is shorter than isoform 1.