RAP1A (NM_002884) Human Untagged Clone

CAT#: SC127673

RAP1A (untagged)-Human RAP1A, member of RAS oncogene family (RAP1A), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RAP1A
• Synonyms: C21KG; G-22K; KREV-1; KREV1; RAP1; SMGP21
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_002884, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCGTGAGTACAAGCTAGTGGTCCTTGGTTCAGGAGGCGTTGGGAAGTCTGCTCTGACAGTTCAGTTTG
  TTCAGGGAATTTTTGTTGAAAAATATGACCCAACGATAGAAGATTCCTACAGAAAGCAAGTTGAAGTCGA
  TTGCCAACAGTGTATGCTCGAAATCCTGGATACTGCAGGGACAGAGCAATTTACAGCAATGAGGGATTTG
  TATATGAAGAACGGCCAAGGTTTTGCACTAGTATATTCTATTACAGCTCAGTCCACGTTTAACGACTTAC
  AGGACCTGAGGGAACAGATTTTACGGGTTAAGGACACGGAAGATGTTCCAATGATTTTGGTTGGCAATAA
  ATGTGACCTGGAAGATGAGCGAGTAGTTGGCAAAGAGCAGGGCCAGAATTTAGCAAGACAGTGGTGTAAC
  TGTGCCTTTTTAGAATCTTCTGCAAAGTCAAAGATCAATGTTAATGAGATATTTTATGACCTGGTCAGAC
  AGATAAATAGGAAAACACCAGTGGAAAAGAAGAAGCCTAAAAAGAAATCATGTCTGCTGCTCTAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002884 unedited
  NGGTTCACATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACCAGGGGATCGTC
  AGTATTTAAACAGATCACATCATGCGTGAGTACAAGCTAGTGGTCCTTGGTTCAGGAGGC
  GTTGGGAAGTCTGCTCTGACAGTTCAGTTTGTTCAGGGAATTTTTGTTGAAAAATATGAC
  CCAACGATAGAAGATTCCTACAGAAAGCAAGTTGAAGTCGATTGCCAACAGTGTATGCTC
  GAAATCCTGGATACTGCAGGGACAGAGCAATTTACAGCAATGAGGGATTTGTATATGAAG
  AACGGCCAAGGTTTTGCACTAGTATATTCTATTACAGCTCAGTCCACGTTTAACGACTTA
  CAGGACCTGAGGGAACAGATTTTACGGGTTAAGGACACGGAAGATGTTCCAATGATTTTG
  GTTGGCAATAAATGTGACCTGGAAGATGAGCGAGTAGTTGGCAAAGAGCAGGGCCAGAAT
  TTAGCAAGACAGTGGTGTAACTGTGCCTTTTTTAGAATCTTCTGCAAAGTCAAAGATCAA
  TGTTAATGAGATATTTTATGACCTGGTCAGACAGATAAATAGGAAAACACCAGTGGAAAA
  GAAGAAGCCTAAAAAGAAATCATGTCTGCTGCTCTAGGCCCATAGTCAGCAGCAGCTCTG
  AGCCAGATTACAGGAATGAAGAACTGTTGCCTAATTGGAAAGTGCCAGCATTCCAGACTT
  CAAAAATAAAATCTGAGAGGCTTCTNCTGTTTATATATATGTGAAGATTTAGATCTTATA
  TGGTNNNGCACAGTCCTGGAGAAAAAAATGCTCTGTGTTNTCTCTTGGAAAATAGACATA
  GTTTTTCTCTTTGCATAGCAGTATTACAGATGTGAAATATACTGACTCTATATGATATAC
  AAAGAGCATGGTGCATTTCAATGTTAGATATGCTCTATATCAATGC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002884
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002884.2, NP_002875.1
• RefSeq Size: 1812 bp
• RefSeq ORF: 555 bp
• Locus ID: 5906
• Cytogenetics: 1p13.2
• Domains: ras, RAN, RAS, RHO, RAB
• Protein Families: Druggable Genome
• Protein Pathways: Chemokine signaling pathway, Focal adhesion, Leukocyte transendothelial migration, Long-term potentiation, MAPK signaling pathway, Neurotrophin signaling pathway, Renal cell carcinoma
• Gene Summary: 'This gene encodes a member of the Ras family of small GTPases. The encoded protein undergoes a change in conformational state and activity, depending on whether it is bound to GTP or GDP. This protein is activated by several types of guanine nucleotide exchange factors (GEFs), and inactivated by two groups of GTPase-activating proteins (GAPs). The activation status of the encoded protein is therefore affected by the balance of intracellular levels of GEFs and GAPs. The encoded protein regulates signaling pathways that affect cell proliferation and adhesion, and may play a role in tumor malignancy. Pseudogenes of this gene have been defined on chromosomes 14 and 17. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]'
  Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 4. Variants 1-5 encode the same protein.