ADFP (PLIN2) (NM_001122) Human Untagged Clone

CAT#: SC127716

PLIN2 (untagged)-Human perilipin 2 (PLIN2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PLIN2
• Synonyms: ADFP; ADRP
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC127716 sequence for NM_001122 edited (data generated by NextGen Sequencing)
  ATGGCATCCGTTGCAGTTGATCCACAACCGAGTGTGGTGACTCGGGTGGTCAACCTGCCC
  TTGGTGAGCTCCACGTATGACCTCATGTCCTCAGCCTATCTCAGTACAAAGGACCAGTAT
  CCCTACCTGAAGTCTGTGTGTGAGATGGCAGAGAACGGTGTGAAGACCATCACCTCCGTG
  GCCATGACCAGTGCTCTGCCCATCATCCAGAAGCTAGAGCCGCAAATTGCAGTTGCCAAT
  ACCTATGCCTGTAAGGGGCTAGACAGGATTGAGGAGAGACTGCCTATTCTGAATCAGCCA
  TCAACTCAGATTGTTGCCAATGCCAAAGGCGCTGTGACTGGGGCAAAAGATGCTGTGACG
  ACTACTGTGACTGGGGCCAAGGATTCTGTGGCCAGCACGATCACAGGGGTGATGGACAAG
  ACCAAAGGGGCAGTGACTGGCAGTGTGGAGAAGACCAAGTCTGTGGTCAGTGGCAGCATT
  AACACAGTCTTGGGGAGTCGGATGATGCAGCTCGTGAGCAGTGGCGTAGAAAATGCACTC
  ACCAAATCAGAGCTGTTGGTAGAACAGTACCTCCCTCTCACTGAGGAAGAACTAGAAAAA
  GAAGCAAAAAAAGTTGAAGGATTTGATCTGGTTCAGAAGCCAAGTTATTATGTTAGACTG
  GGATCCCTGTCTACCAAGCTTCACTCCCGTGCCTACCAGCAGGCTCTCAGCAGGGTTAAA
  GAAGCTAAGCAAAAAAGCCAACAGACCATTTCTCAGCTCCATTCTACTGTTCACCTGATT
  GAATTTGCCAGGAAGAATGTGTATAGTGCCAATCAGAAAATTCAGGATGCTCAGGATAAG
  CTCTACCTCTCATGGGTAGAGTGGAAAAGGAGCATTGGATATGATGATACTGATGAGTCC
  CACTGTGCTGAGCACATTGAGTCACGTACTCTTGCAATTGCCCGCAACCTGACTCAGCAG
  CTCCAGACCACGTGCCACACCCTCCTGTCCAACATCCAAGGTGTACCACAGAACATCCAA
  GATCAAGCCAAGCACATGGGGGTGATGGCAGGCGACATCTACTCAGTGTTCCGCAATGCT
  GCCTCCTTTAAAGAAGTGTCTGACAGCCTCCTCACTTCTAGCAAGGGGCAGCTGCAGAAA
  ATGAAGGAATCTTTAGATGACGTGATGGATTATCTTGTTAACAACACGCCCCTCAACTGG
  CTGGTAGGTCCCTTTTATCCTCAGCTGACTGAGTCTCAGAATGCTCAGGACCAAGGTGCA
  GAGATGGACAAGAGCAGCCAGGAGACCCAGCGATCTGAGCATAAAACTCATTAA
  
  Clone variation with respect to NM_001122.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001122 unedited
  NGGGTCAGATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACCAGGCAGCCGGA
  GTCGTCTTCGGGACGCGCCTGCTCTTCGCCTTTCGCTGCAGTCCGTCGATTTCTTTCTCC
  AGGAAGAAAAAGGCATCCGTTGCAGTTGATCCACAACCGAGTGTGGTGACTCGGGTGGTC
  AACCTGCCCTTGGTGAGCTCCACGTATGACCTCATGTCCTCAGCCTATCTCAGTACAAAG
  GACCAGTATCCCTACCTGAAGTCTGTGTGTGAGATGGCAGAGAACGGTGTGAAGACCATC
  ACCTCCGTGGCCATGACCAGTGCTCTGCCCATCATCCAGAAGCTAGAGCCGCAAATTGCA
  GTTGCCAATACCTATGCCTGTAAGGGGCTAGACAGGATTGAGGAGAGACTGCCTATTCTG
  AATCAGCCATCAACTCAGATTGTTGCCAATGCCAAAGGCGCTGTGACTGGGGCAAAAGAT
  GCTGTGACGACTACTGTGACTGGGGCCAAGGATTCTGTGGCCAGCACGATCACAGGGGTG
  ATGGACAAGACCAAAGGGGCAGTGACTGGCAGTGTGGAGAAGACCAAGTCTGTGGTCAGT
  GGCAGCATTAACACAGTCTTGGGGAGTCGGATGATGCAGCTCGTGAGCAGTGGCGTAGAA
  AATGCACTCACCAAATCAGAGCTGTTGGTAGAACAGTACCTCCCTCTCACTGAGGAAGAA
  CTAGAAAAAGAAGCAAAAAAAGTTGAAGGGATTTGATCTGGTTCAGAAGCCAAGTTATTA
  TGTTAGACTGGGATCCCTGTCTACCAAGCTTCACTCCCGTGCCTACCAGCANGCTCTCAG
  CAGGGTTAAAGAAGCTAAGCANAAAAGCCACAGACCATTTCTCAGCTCATTCTACTGNTC
  ACCTGATA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001122 unedited
  NTTCACTCTGGACCGGCGGCCGCATNCTANGATCGAGTTTTTTTTTTTTTTTTTTTTATC
  CTGCAGTGAATTTTATTGAATTCAAGGTAGTATTCCTATTTGAGAAATGAAAAACTAAAA
  TCAAGTTTTACAAGCAAGCATCATGAGATAAAGCAGTGAAGACGCCTTTTCAGATCACAC
  CAGAGCAGACACCAGTTTCTACCCCAGCCCACATGAAGATGTTTTTATTCAATACAAACA
  GTAACAGAGGCAACACAATGGCCATAGAATGAGAACATAAGTGCATTTGAATTTTTTCTG
  AGTTCTGGCTATAGGCTCTGACAAGCCTATCATTCTTTTCTTACCAGGTGAACAGAAATC
  ATCTGCTAATGCCAGAAACTTTAAAGCTCTTAATTCAGCTGGAAACAACTACAATTGAGG
  GCCTTTATACTAGCTACTTGCTTCCCAATTTAGGGTTGCCTAGCAAGTTAATTTCAACAT
  AACAAAAGGTGTCATCTGTCTGGCCACAGCATGCACTAGTGATAGGGGCAGGTTTAATGA
  GTTTTATGCTCAGATCGCTGGGTCTCCTGGCTGCTCTTGTCCATCTCTGCACCTTGGTCC
  TGAGCATTCTGAGACTCAGTCAGCTGAGGATAAAAGGGACCTACCAGCCAGTTGAGGGGG
  CGTGTTGTTAACAAGATAATCCATCACGTCATCTAAAGATTCCTTCATTTTCTGCAGCTG
  CCCCTTGCTAGAAGTGAGGAGGCTGTCAGACACTTCTTTAAAGGGAGCAGCATTGCGGGA
  CACTGAGTAGATGTCGCCTGCCATCACCCCCATGTGCTTGGCTTGGATCTGGGATGTCTG
  TGGTACACCTTGGNATGTGGACAGGAGGGTGTGGCACGTGGTCTGGAGCTGCTGAGTCAG
  GTTGCGGGCNAATGCAGAATACGTGACTCATGTGCTCAGACAGTGGGACTCATCA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001122
• Insert Size: 1950 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001122.2, NP_001113.2
• RefSeq Size: 2010 bp
• RefSeq ORF: 1314 bp
• Locus ID: 123
• Cytogenetics: 9p22.1
• Domains: perilipin
• Protein Families: Druggable Genome
• Gene Summary: 'The protein encoded by this gene belongs to the perilipin family, members of which coat intracellular lipid storage droplets. This protein is associated with the lipid globule surface membrane material, and maybe involved in development and maintenance of adipose tissue. However, it is not restricted to adipocytes as previously thought, but is found in a wide range of cultured cell lines, including fibroblasts, endothelial and epithelial cells, and tissues, such as lactating mammary gland, adrenal cortex, Sertoli and Leydig cells, and hepatocytes in alcoholic liver cirrhosis, suggesting that it may serve as a marker of lipid accumulation in diverse cell types and diseases. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2011]'
  Transcript Variant: This variant (1) represents the protein-coding transcript.