Carboxypeptidase M (CPM) (NM_001874) Human Untagged Clone

CAT#: SC127763

CPM (untagged)-Human carboxypeptidase M (CPM), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CPM
• Synonyms: carboxypeptidase M
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC127763 sequence for NM_001874 edited (data generated by NextGen Sequencing)
  ATGGACTTCCCGTGCCTCTGGCTAGGGCTGTTGCTGCCTTTGGTAGCTGCGCTGGATTTC
  AACTACCACCGCCAGGAAGGGATGGAAGCGTTTTTGAAGACTGTTGCCCAAAACTACAGT
  TCTGTCACTCACTTACACAGTATTGGGAAATCTGTGAAAGGTAGAAACCTGTGGGTTCTT
  GTTGTGGGGCGGTTTCCAAAGGAACACAGAATTGGGATTCCAGAGTTCAAATACGTGGCA
  AATATGCATGGAGATGAGACTGTTGGGCGGGAGCTGCTGCTCCATCTGATTGACTATCTC
  GTAACCAGTGATGGCAAAGACCCTGAAATCACAAATCTGATCAATAGTACCCGGATACAC
  ATCATGCCTTCCATGAACCCAGATGGATTTGAAGCCGTCAAAAAGCCTGACTGTTACTAC
  AGCATCGGAAGGGAAAATTATAACCAGTATGACTTGAATCGAAATTTCCCCGATGCTTTT
  GAATATAATAATGTCTCAAGGCAGCCTGAAACTGTGGCAGTCATGAAGTGGCTGAAAACA
  GAGACGTTTGTCCTCTCTGCAAACCTCCATGGTGGTGCCCTCGTGGCCAGTTACCCATTT
  GATAATGGTGTTCAAGCAACTGGGGCATTATACTCCCGAAGCTTAACGCCTGATGATGAT
  GTTTTTCAATATCTTGCACATACCTATGCTTCAAGAAATCCCAACATGAAGAAAGGAGAC
  GAGTGTAAAAACAAAATGAACTTTCCTAATGGTGTTACAAATGGATACTCTTGGTATCCA
  CTCCAAGGTGGAATGCAAGATTACAACTACATCTGGGCCCAGTGTTTTGAAATTACGTTG
  GAGCTGTCATGCTGTAAATATCCTCGTGAGGAGAAGCTTCCATCCTTTTGGAATAATAAC
  AAAGCCTCATTAATTGAATATATAAAGCAGGTGCACCTAGGTGTAAAGGGTCAAGTTTTT
  GATCAGAATGGAAATCCATTACCCAATGTAATTGTGGAAGTCCAAGACAGAAAACATATC
  TGCCCCTATAGAACCAACAAATATGGAGAGTATTATCTCCTTCTCTTGCCTGGGTCCTAT
  ATAATAAATGTTACAGTCCCTGGACATGATCCACACATCACAAAGGTGATTATTCCGGAG
  AAATCCCAGAACTTCAGTGCTCTTAAAAAGGATATTCTACTTCCATTCCAAGGGCAATTG
  GATTCTATCCCAGTATCAAATCCTTCATGCCCAATGATTCCTCTATACAGAAATTTGCCA
  GACCACTCAGCTGCAACAAAGCCTAGTTTGTTCTTATTTTTAGTGAGTCTTTTGCACATA
  TTCTTCAAATAA
  
  Clone variation with respect to NM_001874.4
  417 t=>c;1077 t=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001874 unedited
  TACTATAGGGCGGCCGCGAATTCGGCACGAGGGCGCGCCTGGGACCTGAACATGGACTTC
  CCGTGCCTCTGGCTAGGGCTGTTGCTGCCTTTGGTAGCTGCGCTGGATTTCAACTACCAC
  CGCCAGGAAGGGATGGAAGCGTTTTTGAAGACTGTTGCCCAAAACTACAGTTCTGTCACT
  CACTTACACAGTATTGGGAAATCTGTGAAAGGTAGAAACCTGTGGGTTCTTGTTGTGGGG
  CGGTTTCCAAAGGAACACAGAATTGGGATTCCAGAGTTCAAATACGTGGCAAATATGCAT
  GGAGATGAGACTGTTGGGCGGGAGCTGCTGCTCCATCTGATTGACTATCTCGTAACCAGT
  GATGGCAAAGACCCTGAAATCACAAATCTGATCAATAGTACCCGGATACACATCATGCCT
  TCCATGAACCCAGATGGATTTGAAGCCGTCAAAAAGCCTGACTGTTACTACAGCATCGGA
  AGGGAAAATTATAACCAGTATGACTTGAATCGAAATTTCCCCGATGCTTTTGAATATAAT
  AATGTCTCAAGGCAGCCTGAAACTGTGGCAGTCATGAAGTGGCTGAAACAGAGACGTTTG
  TCCTCTCTGCANACTNCATGGTGGTGCCCTCGTGGCCAGTTACCCATTTGATAATGGTGT
  TCAAGCAACTGGNGCATTATACTCCCGAAGCTAACGCCTGATGATGATGTTTTTCAATAT
  CTGCACATACCTATGCTTCAGAAATCCCCACATGAGAAGGAGACGAGTGTAAAACANATG
  AACTTTNCTATGGTGTACAATGGATACTCTGGTATCACTCCAGGTGGATGCAGATACACT
  ACTCTGGCCCATGTTTGGAATACCGTGACTGCATGCGNAATTCCTGGAGAGAGCTNCATC
  TTTGATATACAAGCTATATGAATTAAGCAGGCCCTAGGTAGGGCAGNTTTGTCATGGATA
  CTACCATGATTGGGAC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001874 unedited
  GAACCGCGGCACGCAATCTAGTGTCGAGTTTTTTTTTTTTTTTTTTAGTAAAGATGGGGT
  TTCACCATGTTGGCCAGGCTGGTCTTGAGCTCCTGACCTCAAGTGATCCGCCCGCCTTGG
  CCTCCCAAAATGCTGGGATTACAGGCATGAGCCACCATGCCTGGCCAACACCAACCTTTA
  GTTACTTGCTGAATACACAGGATTAAGGAGGAAGAGAAAGTATCAAGGATGACTCCCAGG
  TTCCTAGCTTGGTGGTCCCAGTAATTGAGAGAGAGAATGCAGCAGGAAGAGCAGCTCTAG
  GGGGAACAGGATGGGTATCTCCACATGATGGGTTGGAGGTACCTGTGAGTCACATCCAGG
  TGGATGTGTCCTGTGGGCAGTGGGACGCGAGTCTGAAGCTCAAGCAAGAGGCTAGAGTTA
  CATCTTTGGAGTCATCAGCCTAATGGAGGACTGTGGCATCCAGGTGCCAAAAGCTAGGGA
  AACGGCTCTGGAGAGAGAGGGGCTTTCTTAATTGACCTGTATGCTTTGAAGCTCTATAGA
  GACACAATCTTATCCCTTCAAGCCTGTATTTTCTTGGGCCACTAAGATGTGGTTACATAT
  TTTCTCTCACTTCTCCTAGAACGTTTGCAAAACAATTCCCAGTCCTATGATCATTTTCTC
  CCTTCTTCTGAGAACCTATTATATATGGCACTTAATTTCTATTCCCATGTAACACTCATG
  GAGAGGGGGTCTTTTTCGTTGGTAGGTCTTGGTTTTTTTTTTTGAAACAAAAACTAACCT
  TCTGGCCAATCCGGAACCCAAGGCACTATCTCGGCTTACCGAAAACCCGCCTTTAAGGTT
  AAGCCATTCTTCCGTTTTATCCCCCATAAGGCGCGAATTCAAGTGCCTGCACTAACCCGG
  TAATTTCACAAAAACAGGGCTCCACTTGTGCTCTCTCGTCCTAATCCCTGACCATGGTCA
  CACCCCTTTCACCTCCAATATCTATATCAACTGGTAACTCCCTTCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001874
• Insert Size: 2800 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001874.3, NP_001865.1
• RefSeq Size: 6683 bp
• RefSeq ORF: 1332 bp
• Locus ID: 1368
• Cytogenetics: 12q15
• Domains: Zn_carbOpept
• Protein Families: Druggable Genome, Protease
• Gene Summary: 'The protein encoded by this gene is a membrane-bound arginine/lysine carboxypeptidase. Its expression is associated with monocyte to macrophage differentiation. This encoded protein contains hydrophobic regions at the amino and carboxy termini and has 6 potential asparagine-linked glycosylation sites. The active site residues of carboxypeptidases A and B are conserved in this protein. Three alternatively spliced transcript variants encoding the same protein have been described for this gene. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) differs in the 5' UTR compared to variants 2 and 3. All three variants encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no quality transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.