PURA (NM_005859) Human Untagged Clone

CAT#: SC127792

PURA (untagged)-Human purine-rich element binding protein A (PURA)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PURA
• Synonyms: MRD31; PUR-ALPHA; PUR1; PURALPHA
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_005859, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCGGACCGAGACAGCGGCAGCGAGCAGGGTGGTGCGGCGCTGGGTTCGGGCGGCTCCCTGGGGCACC
  CCGGCTCGGGCTCAGGCTCCGGCGGGGGCGGTGGTGGCGGCGGGGGCGGCGGCGGCAGTGGCGGCGGCGG
  CGGCGGGGCCCCAGGGGGGCTGCAGCACGAGACGCAGGAGCTGGCCTCCAAGCGGGTGGACATCCAGAAC
  AAGCGCTTCTACCTGGACGTGAAGCAGAACGCCAAGGGCCGCTTCCTGAAGATCGCCGAGGTGGGCGCGG
  GCGGCAACAAGAGCCGCCTTACTCTCTCCATGTCAGTGGCCGTGGAGTTCCGCGACTACCTGGGCGACTT
  CATCGAGCACTACGCGCAGCTGGGCCCCAGCCAGCCGCCGGACCTGGCCCAGGCGCAGGACGAGCCGCGC
  CGGGCGCTCAAAAGCGAGTTCCTGGTGCGCGAGAACCGCAAGTACTACATGGATCTCAAGGAGAACCAGC
  GCGGCCGCTTCCTGCGCATCCGCCAGACGGTCAACCGGGGGCCTGGCCTGGGCTCCACGCAGGGCCAGAC
  CATTGCGCTGCCCGCGCAGGGGCTCATCGAGTTCCGTGACGCTCTGGCCAAGCTCATCGACGACTACGGA
  GTGGAGGAGGAGCCGGCCGAGCTGCCCGAGGGCACCTCCTTGACTGTGGACAACAAGCGCTTCTTCTTCG
  ATGTGGGCTCCAACAAGTACGGCGTGTTTATGCGAGTGAGCGAGGTGAAGCCCACCTATCGCAACTCCAT
  CACCGTGCCCTACAAGGTGTGGGCCAAGTTCGGACACACCTTCTGCAAGTACTCGGAGGAGATGAAGAAG
  ATTCAAGAGAAGCAGAGGGAGAAGCGGGCTGCCTGTGAGCAGCTTCACCAGCAGCAACAGCAGCAGCAGG
  AGGAGACCGCCGCTGCCACCCTGCTACTGCAGGGTGAGGAAGAAGGGGAAGAAGATTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005859 unedited
  CTATAGGCGGCCGCGCAGCGGCGGCGGCGCGGCAGCGGAGCGCAGCATCATGGCGGACCG
  AGACAGCGGCAGCGAGCAGGGTGGTGCGGCGCTGGGTTCGGGCGGCTCCCTGGGGCACCC
  CGGCTCGGGCTCAGGCTCCGGCGGGGGCGGTGGTGGCGGCGGGGGCGGCGGCGGCAGTGG
  CGGCGGCGGCGGCGGGGCCCCAGGGGGGCTGCAGCACGAGACGCAGGAGCTGGCCTCCAA
  GCGGGTGGACATCCAGAACAAGCGCTTCTACCTGGACGTGAAGCAGAACGCCAAGGGCCG
  CTTCCTGAAGATCGCCGAGGGGNNCGCGGNCGGCAACAAGAGCCGCCTTACTCTCTCCAT
  GTCAGTGGCCGTGGAGTTCCGCGACTACCTGGGCGACTTCATCGAGCACTACGCGCAGCT
  GGGCCCCAGCCAGCCGCCGGACCTGGCCCAGGCGCAGGACGAGCCGCGCCGGGCGCTCAA
  AAGCGAGTTCCTGGTGCGCGAGAACCGCAAGTACTACATGGATCTCAAGGAGAACCAGCG
  CGGCCGCTTCCTGCGCATCCGCCAGACGGTCAACCGGGGGCCTGGCCTGGGCTCCACGCA
  GGGCCAGACCATTGCGCTGCCCGCGCAGGGGCTCATCGA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005859 unedited
  TTTAAGGTCTTCTATGNACCGCGCCGCATTCTATGATCGGTTTTTTTTTTTTTTTTTTTT
  TTGTTCTTTATATTTTTTAATCAAGTAGGGAAACACAGTTCATATAATGTTATTACTTTT
  TGTATTTTTTTTGTTTTTAGCAGCTGCTTACTGTTTTATATGGTGGATAAAGTGGACAAC
  ATCCAGCGATGGGTGGCAAGTCAAGAAGAGAAACTGTCAGTTGGTGGAAGTTCTGTGGGT
  GGTGCTCCCTTGGAGTTAACAAGTTTTTTTTTTTTTTTAACTTTTTAACTTTTTATTTTC
  TCTCTTTCTTTACAGTATATTTTCTGTGTGTGTGGCTGTGTGTGTGTGTGTATGCATGTG
  TGTGTGTGTGGGGGTTTCATTCAGTTTGATCAATCTTCTTCCCCTTCTTCCTCACCCTGC
  AGTAGCAGGGTGGCAGCGGCGGTCTCCTCCTGCTGCTGCTGTTGCTGCTGGTGAAACTGC
  TCACAGGCAGCCCGCTTCTCCCTCTGCTTCTCTTGAATCTTCTTCATCTCCTCCGAGTAC
  TTGCAGAAAGTGTGTCCGAACTTGGCCCACACCTTGTAGGGCACGGTGATGGAGTTGCGA
  TAGGTGGGCTTCACCTCGCTCACTCGCATAAACACGCCGTACTTGTTGGAGCCCACATCG
  AAGAATAAGCGCTTGTTGTCCACAGTCAAAGAGGTGCCCTCGGGCAGCTCGGCCGGCTCC
  TCCTCCACTCCGTATTCCTCCATGAGCTTGGCCAGAGCGTCACGGGACTCGATGAGCCCC
  TGCGCGGGCAGCGCAATGGTCTGGCCTTGCGTGGAGCCCAGCCAGGCCCCCGGTTGACCC
  TCTGGGCGATGCGCCAGAATCCGGCCCCCCTGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005859
• Insert Size: 1500 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005859.3, NP_005850.1
• RefSeq Size: 2614 bp
• RefSeq ORF: 969 bp
• Locus ID: 5813
• Cytogenetics: 5q31.3
• Domains: PUR
• Protein Families: Transcription Factors
• Gene Summary: 'This gene product is a sequence-specific, single-stranded DNA-binding protein. It binds preferentially to the single strand of the purine-rich element termed PUR, which is present at origins of replication and in gene flanking regions in a variety of eukaryotes from yeasts through humans. Thus, it is implicated in the control of both DNA replication and transcription. Deletion of this gene has been associated with myelodysplastic syndrome and acute myelogenous leukemia. [provided by RefSeq, Jul 2008]'