Galectin 8 (LGALS8) (NM_006499) Human Untagged Clone

CAT#: SC127815

LGALS8 (untagged)-Human lectin, galactoside-binding, soluble, 8 (LGALS8), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: LGALS8
• Synonyms: Gal-8; PCTA-1; PCTA1; Po66-CBP
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_006499, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGATGTTGTCCTTAAACAACCTACAGAATATCATCTATAACCCGGTAATCCCGTTTGTTGGCACCATTC
  CTGATCAGCTGGATCCTGGAACTTTGATTGTGATACGTGGGCATGTTCCTAGTGACGCAGACAGATTCCA
  GGTGGATCTGCAGAATGGCAGCAGCATGAAACCTCGAGCCGATGTGGCCTTTCATTTCAATCCTCGTTTC
  AAAAGGGCCGGCTGCATTGTTTGCAATACTTTGATAAATGAAAAATGGGGACGGGAAGAGATCACCTATG
  ACACGCCTTTCAAAAGAGAAAAGTCTTTTGAGATCGTGATTATGGTGCTGAAGGACAAATTCCAGGTGGC
  TGTAAATGGAAAACATACTCTGCTCTATGGCCACAGGATCGGCCCAGAGAAAATAGACACTCTGGGCATT
  TATGGCAAAGTGAATATTCACTCAATTGGTTTTAGCTTCAGCTCGGACTTACAAAGTACCCAAGCATCTA
  GTCTGGAACTGACAGAGATAAGTAGAGAAAATGTTCCAAAGTCTGGCACGCCCCAGCTTCCTAGTAATAG
  AGGAGGAGACATTTCTAAAATCGCACCCAGAACTGTCTACACCAAGAGCAAAGATTCGACTGTCAATCAC
  ACTTTGACTTGCACCAAAATACCACCTATGAACTATGTGTCAAAGAGGCTGCCATTCGCTGCAAGGTTGA
  ACACCCCCATGGGCCCTGGACGAACTGTCGTCGTTAAAGGAGAAGTGAATGCAAATGCCAAAAGCTTTAA
  TGTTGACCTACTAGCAGGAAAATCAAAGGATATTGCTCTACACTTGAACCCACGCCTGAATATTAAAGCA
  TTTGTAAGAAATTCTTTTCTTCAGGAGTCCTGGGGAGAAGAAGAGAGAAATATTACCTCTTTCCCATTTA
  GTCCTGGGATGTACTTTGAGATGATAATTTATTGTGATGTTAGAGAATTCAAGGTTGCAGTAAATGGCGT
  ACACAGCCTGGAGTACAAACACAGATTTAAAGAGCTCAGCAGTATTGACACGCTGGAAATTAATGGAGAC
  ATCCACTTACTGGAAGTAAGGAGCTGGTAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006499 unedited
  TCCCGCCAAGTCGGGATTTGTNACACGACTTACTATAGGGCGGCCGCGAATTCGCACCAG
  CCGCCCACGNCGCCAGAGCCGGGAACCCTGACGGCACTTAGCTGCTGACAAACAACCTGC
  TCCGTGGAGCGCCTGAAACACCAGTCTTTGGGGCCAGTGCCTCAGTTTCAATCCAGGTAA
  CCTTTAAATGAAACTTGCCTAAAATCTTAGGTCATACACAGAAGAGACTCCAATCGACAA
  GAAGCTGGAAAAGAATGATGTTGTCCTTAAACAACCTACAGAATATCATCTATAACCCGG
  TAATCCCGTATGTTGGCACCATTCCCGATCAGCTGGATCCTGGAACTTTGATTGTGATAT
  GTGGGCATGTTCCTAGTGACGCAGACAGATTCCAGGTGGATCTGCAGAATGGCAGCAGCG
  TGAAACCTCGAGCCGATGTGGCCTTTCATTTCAATCCTCGTTTCAAAAGGGCCGGCTGCA
  TTGTTTGCAATACTTTGATAAATGAAAAATGGGGACGGGAAGAGATCACCTATGACACGC
  CTTTCAAAAGAGAAAAGTCTTTTGAGATCGTGATTATGGTGCTCAAGGACAAATTCCAGG
  TGGGCTGTAAATGGAAAACATACTCTGCTCTATGGCCACAAGATCGGCCCACAAAAAATA
  GACACTCTGGGGCATTTATGGCAAAGTGAATATTCACTCCAATTGGTTTTAGCTTTTACT
  TCGGACTTTCAAAGTACCCCAAGCTCTTAATCTGGACCTGACCGATATAAGTTGAGAAAA
  AGTTTCCAAAGTTGGGCACCCCCCAACTTAACCCTGCCATTCCCTGCCAGGTGGAACCCC
  CCCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006499 unedited
  TTTTGGAATTACTATGNACGCGGCCGCTTTCTANGATCGAGTTTTTTTTTTTTTTTTTTA
  ACTTGCTGATACAGGTTTGATTTTAATCACGTAAAGTGGAAGAGTGCTTTGATCTCAGTT
  GCTAATGATGATGGACTAATCTTTAGAAGAACGGAGGCCTTGATTGGTGATTTCATGGGA
  ACAAAACAGATTTCTGCATTACCTCTGCCTGCTTTGTGGGTCCCTTAGTTGGCGTACACT
  TTCTGATAGTGCCTGCCTTATGAATGCCTGAAGTCTAATCCTGACCTTACTTTGTTGCCC
  ATAAATATCTTTCAAATTGAATGCATCTTGCCTCTTCCTGAAACTTTCTGTAGGCGTCTG
  TGTAGACTCTGATTTGCCCTAAGTTCAAATACACAGAAATCAGCTGCCTTGGGCTTGGCA
  TCAAGTTAATTTCAGCACCATGTATTTGTTTTGCTTTGGTTTTTTGCCTTAATTTCTGTG
  CTCAGATGTCATGCCAATTACTTGGTAAAGCTTGCAGCTAATAGAGCCACATGTTTATTA
  CGATAAAATGATGTCAGTCAACATCAGTCCTCAGTGATGGGATACGGTAGAGCACTGGTT
  ATGACAGTGATCACTGGGGAAGGAGTTGTGTCTTCTGTAAATTAGTGTCTTCGGGTGATT
  TAATAGCTGCTTAACTAATCCCAGAGGACCCCTTNAGAGTTGATGTAATGCCTGTAGAAA
  AAGCAGTGCGCAGCAATAGAGCACAGAAGAGTCGAAGCTCAAGAGAACAGGGAACATACT
  GGTTTGTTTCACCAGTATATATCCTAGCTCCTCACAGTTTTACCAAAAAAAATCTGTGCT
  CAATACATGTTAGCTACTGTATTATAGTAGGTACTCCATN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_006499
• Insert Size: 2550 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006499.3, NP_006490.3
• RefSeq Size: 2996 bp
• RefSeq ORF: 1080 bp
• Locus ID: 3964
• Cytogenetics: 1q43
• Domains: Gal-bind_lectin
• Gene Summary: 'This gene encodes a member of the galectin family. Galectins are beta-galactoside-binding animal lectins with conserved carbohydrate recognition domains. The galectins have been implicated in many essential functions including development, differentiation, cell-cell adhesion, cell-matrix interaction, growth regulation, apoptosis, and RNA splicing. This gene is widely expressed in tumoral tissues and seems to be involved in integrin-like cell interactions. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) represents the longest transcript. Variants 1 and 4 encode the longer isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. CCDS Note: This CCDS representation uses the 5'-most in-frame start codon, which is conserved in higher primates. An alternative downstream start codon, which is more widely conserved, also exists. It is possible that leaky scanning by ribosomes would allow the downstream start codon to be used, at least some of the time. The use of the downstream start codon would result in a protein that is 1 aa shorter at the N-terminus. There is no experimental evidence showing which start codon is preferentially used in vivo.