SOD2 (NM_000636) Human Untagged Clone

CAT#: SC127816

SOD2 (untagged)-Human superoxide dismutase 2, mitochondrial (SOD2), nuclear gene encoding mitochondrial protein, transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SOD2
• Synonyms: GClnc1; IPO-B; IPOB; Mn-SOD; MNSOD; MVCD6
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC127816 sequence for NM_000636 edited (data generated by NextGen Sequencing)
  ATGTTGAGCCGGGCAGTGTGCGGCACCAGCAGGCAGCTGGCTCCGGTTTTGGGGTATCTG
  GGCTCCAGGCAGAAGCACAGCCTCCCCGACCTGCCCTACGACTACGGCGCCCTGGAACCT
  CACATCAACGCGCAGATCATGCAGCTGCACCACAGCAAGCACCACGCGGCCTACGTGAAC
  AACCTGAACGTCACCGAGGAGAAGTACCAGGAGGCGTTGGCCAAGGGAGATGTTACAGCC
  CAGATAGCTCTTCAGCCTGCACTGAAGTTCAATGGTGGTGGTCATATCAATCATAGCATT
  TTCTGGACAAACCTCAGCCCTAACGGTGGTGGAGAACCCAAAGGGGAGTTGCTGGAAGCC
  ATCAAACGTGACTTTGGTTCCTTTGACAAGTTTAAGGAGAAGCTGACGGCTGCATCTGTT
  GGTGTCCAAGGCTCAGGTTGGGGTTGGCTTGGTTTCAATAAGGAACGGGGACACTTACAA
  ATTGCTGCTTGTCCAAATCAGGATCCACTGCAAGGAACAACAGGCCTTATTCCACTGCTG
  GGGATTGATGTGTGGGAGCACGCTTACTACCTTCAGTATAAAAATGTCAGGCCTGATTAT
  CTAAAAGCTATTTGGAATGTAATCAACTGGGAGAATGTAACTGAAAGATACATGGCTTGC
  AAAAAGTAA
  
  Clone variation with respect to NM_000636.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000636 unedited
  GCGGCCGCGAATTCGGCACCAGTGCTCCCCGCGCTTTCTTAAGGCCCGCGGGCGGCGCAG
  GAGCGGCACTCGTGGCTGTGGTGGCTTCGGCAGCGGCTTCAGCAGATCGGCGGCATCAGC
  GGTAGCACCAGCACTAGCAGCATGTTGAGCCGGGCAGTGTGCGGCACCAGCAGGCAGCTG
  GCTCCGGTTTTGGGGTATCTGGGCTCCAGGCAGAAGCACAGCCTCCCCGACCTGCCCTAC
  GACTACGGCGCCCTGGAACCTCACATCAACGCGCAGATCATGCAGCTGCACCACAGCAAG
  CACCACGCGGCCTACGTGAACAACCTGAACGTCACCGAGGAGAAGTACCAGGAGGCGTTG
  GCCAAGGGAGATGTTACAGCCCAGATAGCTCTTCAGCCTGCACTGAAGTTCAATGGTGGT
  GGTCATATCAATCATAGCATTTTCTGGACAAACCTCAGCCCTAACGGTGGTGGAGAACCC
  AAAGGGGAGTTGCTGGAAGCCATCAAACGTGACTTTGGTTCCTTTGACAAGTTTAAGGAG
  AAGCTGACGGCTGCATCTGTTGGTGTCCAGGCTCAGGTTGGGGTTGGCTTGGTTTCATAA
  GGAACGGNGACACTTACAAATTGCTGCTTGTCCAATCAGGATCCACTGCAGGGAACACAG
  GCCTTATTCCACTGCTGGGGATTNGATGTGTGGGAGCACGCTTACTACCTTTCAGTATAA
  ATGTCAGGCCTGA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000636 unedited
  CCCCATTCGTTTGGACCGTCGGCCGCAATCTACGATCGAGTTTTTTTTTTTTTTTTTTGA
  TGGTTGACAGATTCTTTTATTAACAGTCAAAAACTTCACACAGATTGGAAAATAAATGTT
  TTCTTCAATGAATAATCAAACAAAAATTATCCAGGACCTTATAGGGTTTTCAGTATGTAC
  CAGGCTTGATGCACATCTTAGAAGACAGGACATTATCTTGCTGGGATCATTAGGGTATGA
  CTGAAAGTGAGAAACAGTAATTTGTAAAACATTTACCTAATAATAGCTTTCCCAAACAGT
  ACTTCCCCTGGAATTAAAACAGGAAATACAATTTATGTTTATACGTGGTTTTAGATATGA
  CTCAATATACATAGATTTTAACTTTATGGTTTGGTATTACTTTTTTAAAGGCGCTCAATA
  GAAATTCAAATCTCACTTTAAGACCATGAATTTCAAGTTGCAATGAAGTGTACAATAAAG
  TTGTGATTTCTCAACATCAAAGTTTAATTATTACAAAATAGTTCAAGCAACAGATAGAAT
  TTCAAAAACAGTATTTGCTTTGCTTCCTTGTTTTGCTCCAACACTAATCATGCTGAGGTT
  TTTGAAGCACAGCTATGACTAGGGCAGGCACTGCTGATTCAGTCACAAAAACCCTTCTTG
  GATGAACAATACTTGGTCTTTTCAGAAGAAAAGCCATTTTACCTTTTCTATTTCTATTAT
  GAAAAACAGAGCTAAACAATTTTTGTATTTTTGTTGAAGAACCGGGGTTTTACCCCGTTT
  GGCCACCGCTGGTCCCGAACATCCTTAAGGGGTTTTGCCTGCCCTTGGCTTCCCAAGGGT
  TTGGATCACAAAGGCGAGTTACCCCGCCCAGCCCTTAACAATTTTAAAGGTCTCTTTTTC
  CCCACAAGCATTAAACTCTTAGTTGGCAAACCCCTTAACCCTA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000636
• Insert Size: 4300 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000636.2, NP_000627.2
• RefSeq Size: 1593 bp
• RefSeq ORF: 669 bp
• Locus ID: 6648
• Cytogenetics: 6q25.3
• Domains: sodfe
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Huntington's disease
• Gene Summary: 'This gene is a member of the iron/manganese superoxide dismutase family. It encodes a mitochondrial protein that forms a homotetramer and binds one manganese ion per subunit. This protein binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. Mutations in this gene have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer. Alternative splicing of this gene results in multiple transcript variants. A related pseudogene has been identified on chromosome 1. [provided by RefSeq, Apr 2016]'
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (A). Both variants 1 and 2 encode the same isoform (A). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.