Interferon beta (IFNB1) (NM_002176) Human Untagged Clone

CAT#: SC127861

IFNB1 (untagged)-Human interferon, beta 1, fibroblast (IFNB1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: IFNB1
• Synonyms: IFB; IFF; IFN-beta; IFNB
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC127861 sequence for NM_002176 edited (data generated by NextGen Sequencing)
  ATGACCAACAAGTGTCTCCTCCAAATTGCTCTCCTGTTGTGCTTCTCCACTACAGCTCTT
  TCCATGAGCTACAACTTGCTTGGATTCCTACAAAGAAGCAGCAATTTTCAGTGTCAGAAG
  CTCCTGTGGCAATTGAATGGGAGGCTTGAATACTGCCTCAAGGACAGGATGAACTTTGAC
  ATCCCTGAGGAGATTAAGCAGCTGCAGCAGTTCCAGAAGGAGGACGCCGCATTGACCATC
  TATGAGATGCTCCAGAACATCTTTGCTATTTTCAGACAAGATTCATCTAGCACTGGCTGG
  AATGAGACTATTGTTGAGAACCTCCTGGCTAATGTCTATCATCAGATAAACCATCTGAAG
  ACAGTCCTGGAAGAAAAACTGGAGAAAGAAGATTTCACCAGGGGAAAACTCATGAGCAGT
  CTGCACCTGAAAAGATATTATGGGAGGATTCTGCATTACCTGAAGGCCAAGGAGTACAGT
  CACTGTGCCTGGACCATAGTCAGAGTGGAAATCCTAAGGAACTTTTACTTCATTAACAGA
  CTTACAGGTTACCTCCGAAACTGA
  
  Clone variation with respect to NM_002176.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002176 unedited
  AAAAGGGTTGCTATTGTAAACGACTCCCTAAGGGCGGCCGCCGAATTCGTCAACATGACC
  CACCAGTGTCTCCTCCAAATTGCTCTCCTGTTGTGCTTCTCAATACGCCCTTTCCAGAGC
  TTCAACCTGCTTGGATTCCTACAAAGAAGCCGCAACTTTCAGTGTCAGAAGCTCCTGTGG
  CAATATGAACGGGAGGCCTTGAATACTGCCTCAAGGACAGGATGAACTTTGACCTCCCTG
  AGGAAGCTTTGCAGCCGCAGCAGTTCCAGAAGGAGGACGCCCGCATTGACCATCTATGAG
  ATGCTCCAGAACATCTTTGCTATTTTCAGACCAAGATTCATCTAGCACTGGCTGGAATTG
  AGACTATTGTTGAGAACCTTCCTTGGCTTATGTCTATCATCAGATAAACCCATTCTTGAA
  GCCAGTCCTGGAAGAAAAACTGGAGAAAGAAGATTTTCACCAGGGGAAAACTCTTGAGCC
  GTCTGCACCTTGAAAAGATATTATTGGGAGGATTCCTGCATTACCTGAAGGCCAAAGGAG
  TACCAGTCACCTTGTGCCTGGACCATAATCAGAGTGGAAATCCTAAGGGAACCTTTTACT
  TTCATTAACAGAACTTACAGGTTACCTCCGAAACTGAAGATCTCCTAACCTGTGCCTCTG
  GGACTGGACAATTGCTTCAAGCATTCTTCAACCAGCAGATGCTNGTTAAGTGACTGATGG
  CTCGACTCTAGNATTGCGACGCNGTCATAGCTGATTACTGAACGATCCCCGGTGGGCATC
  CTGTGACCCTNCCCAGTGCCTCTCTGGGCCTGGGAGTTTGCACTCCAGTGCCCACAGCCC
  TTGTCTAATAAAATTAAGTGCATCATTTTGTCTGACATAGTGTCCTCTTAATATGGTGGN
  NGGGGGGGGGGGGGGNNNNNNNNNNNNGGGGNNNNNTTTAAAAAAAACAACTGGGGGGCG
  A
  
  3' Read Nucleotide Sequence

  >OriGene 3' genomic read for NM_002176 unedited
  CCAAATCCATTAAACGCATCTGCTGGTTGAAGAATGCTTGAAGCAATTGTCCAGTCCCAG
  AGGCACAGGCTAGGAGATCTTCAGTTTCGGAGGTAACCTGTAAGTCTGTTAATGAAGTAA
  AAGTTCCTTAGGATTTCCACTCTGACTATGGTCCAGGCACAGTGACTGTACTCCTTGGCT
  TTTATGTAATGCAGAATCCTCCCATAATATCTTTTCAGGTGCAGACTGCTCATGAGTTTT
  CCCCTGGTGAAATCTTCTTTCTCCAGTTTTTCTTCCAGGACTGTCTTCAGATGGTTTATC
  TGATGATAGACATTAGCCAGGAGGTTCTCAACAATAGTCTCATTCCAGCCAGTGCTAGAT
  GAATCTTGTCTGAAAATAGCAAAGATGTTCTGGAGCATCTCATAGATGGTCAATGCGGCG
  TCCTCCTTCTGGAACTGCTGCAGCTGCTTAATCTCCTCAGGGATGTCAAAGTTCATCCTG
  TCCTTGAGGCAGTATTCAAGCCTCCCATTCAATTGCCACAGGAGCTTCTGACACTGAAAA
  TTGCTGCTTCTTTGTAGGAATCCAAGCAAGTTGTAGCTCATGGAAAGAGCTGTAGTGGAG
  AAGCACAACAGGAGAGCAATTTGGAGGAGACACTTGTTGGTCATGTTGACGAATTCGCGG
  CCGCCCTATAGTGAGTCGTATTACAAAATTCTGACGGTTCACTAAACGAGCTCTGCTTAT
  ATAGACCTCCCACCGTACACGCCTACCGNCCATTTGCGTCAACGGGGCGGGGTTATTACG
  ACATTTTTGGAAAGTCCCGTTGATTTTTGGTGCCCAACAAACTCCCCATGACGTCCATGG
  GGTGGAGACTTTGGAATCCCCGTGAGTCAAA
  
• Restriction Sites: Please inquire
• ACCN: NM_002176
• Insert Size: 750 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002176.2, NP_002167.1
• RefSeq Size: 840 bp
• RefSeq ORF: 564 bp
• Locus ID: 3456
• Cytogenetics: 9p21.3
• Protein Families: Druggable Genome, Secreted Protein, Transmembrane
• Protein Pathways: Cytokine-cytokine receptor interaction, Cytosolic DNA-sensing pathway, Jak-STAT signaling pathway, Natural killer cell mediated cytotoxicity, RIG-I-like receptor signaling pathway, Toll-like receptor signaling pathway
• Gene Summary: 'This gene encodes a cytokine that belongs to the interferon family of signaling proteins, which are released as part of the innate immune response to pathogens. The protein encoded by this gene belongs to the type I class of interferons, which are important for defense against viral infections. In addition, type I interferons are involved in cell differentiation and anti-tumor defenses. Following secretion in response to a pathogen, type I interferons bind a homologous receptor complex and induce transcription of genes such as those encoding inflammatory cytokines and chemokines. Overactivation of type I interferon secretion is linked to autoimmune diseases. Mice deficient for this gene display several phenotypes including defects in B cell maturation and increased susceptibility to viral infection. [provided by RefSeq, Sep 2015]'