GCAT (NM_014291) Human Untagged Clone

CAT#: SC127864

GCAT (untagged)-Human glycine C-acetyltransferase (GCAT), nuclear gene encoding mitochondrial protein, transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GCAT
• Synonyms: KBL
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC127864 sequence for NM_014291 edited (data generated by NextGen Sequencing)
  ATGTGGCCTGGGAACGCCTGGCGCGCCGCACTCTTCTGGGTGCCCCGCGGCCGCCGCGCA
  CAGTCAGCGCTGGCCCAGCTGCGTGGCATTCTGGAGGGGGAGCTGGAAGGCATCCGCGGA
  GCTGGCACTTGGAAGAGTGAGCGGGTCATCACGTCCCGTCAGGGGCCGCACATCCGCGTG
  GACGGCGTCTCCGGAGGAATCCTTAACTTCTGTGCCAACAACTACCTGGGCCTGAGCAGC
  CACCCTGAGGTGATCCAGGCAGGTCTGCAGGCTCTGGAGGAGTTTGGAGCTGGCCTCAGC
  TCTGTCCGCTTTATCTGTGGAACCCAGAGCATCCACAAGAATCTAGAAGCAAAAATAGCC
  CGCTTCCACCAGCGGGAGGATGCCATCCTCTATCCCAGCTGTTATGACGCCAACGCCGGC
  CTCTTTGAGGCCCTGCTGACCCCAGAGGACGCAGTCCTGTCGGACGAGCTGAACCATGCC
  TCCATCATCGACGGCATCCGGCTGTGCAAGGCCCACAAGTACCGCTATCGCCACCTGGAC
  ATGGCCGACCTAGAAGCCAAGCTGCAGGAGGCCCAGAAGCATCGGCTGCGCCTGGTGGCC
  ACTGATGGGGCCTTTTCCATGGATGGCGACATCGCACCCCTGCAGGAGATCTGCTGCCTC
  GCCTCTAGATATGGTGCCCTGGTCTTCATGGATGAATGCCATGCCACTGGCTTCCTGGGG
  CCCACAGGACGGGGCACAGATGAGCTGCTGGGTGTGATGGACCAGGTCACCATCATCAAC
  TCCACCCTGGGGAAGGCCCTGGGTGGAGCATCAGGGGGCTACACGACAGGGCCTGGGCCC
  CTGGTGTCCCTGCTGCGGCAGCGCGCCCGGCCATACCTCTTCTCCAACAGTCTGCCACCT
  GCTGTCGTTGGCTGCGCCTCCAAGGCCCTAGATCTGCTGATGGGGAGTAACACCATTGTC
  CAGTCTATGGCTGCCAAGACCCAGAGGTTCCGTAGTAAGATGGAAGCTGCTGGCTTCACT
  ATCTCGGGAGCCAGTCACCCCATCTGCCCTGTGATGCTGGGTGATGCCCGGCTGGCCTCT
  CGCATGGCGGATGACATGCTGAAGAGAGGCATCTTTGTCATCGGGTTCAGCTACCCCGTG
  GTCCCCAAGGGCAAGGCCCGGATCCGGGTACAGATCTCAGCAGTGCATAGCGAGGAAGAC
  ATTGACCGCTGCGTGGAGGCCTTCGTGGAAGTGGGGCGACTGCACGGGGCACTGCCCTGA
  
  Clone variation with respect to NM_014291.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_014291 unedited
  NGTCAAAATTTGTATACGACTCATATAGGCGGCCGCGATTCCGAGGTAGAGCGATGTGGC
  CTGGGAACGCCTGGCGCGCCGCACTCTTCTGGGTGCCCCGCGGCCGCCGCGCACAGTCAG
  CGCTGGCCCAGCTGCGTGGCATTCTGGAGGGGGAGCTGGAAGGCATCCGCGGAGCTGGCA
  CTTGGAAGAGTGAGCGGGTCATCACGTCCCGTCAGGGGCCGCACATCCGCGTGGACGGCG
  TCTCCGGAGGAATCCTTAACTTCTGTGCCAACAACTACCTGGGCCTGAGCAGCCACCCTG
  AGGTGATCCAGGCAGGTCTGCAGGCTCTGGAGGAGTTTGGAGCTGGCCTCAGCTCTGTCC
  GCTTTATCTGTGGAACCCAGAGCATCCACAAGAATCTAGAAGCAAAAATAGCCCGCTTCC
  ACCAGCGGGAGGATGCCATCCTCTATCCCAGCTGTTATGACGCCAACGCCGGCCTCTTTG
  AGGCCCTGCTGACCCCAGAGGACGCAGTCCTGTCGGACGAGCTGAACCATGCCTCCATCA
  TCGACGGCATCCGGCTGTGCAAGGCCCACAAGTACCGCTATCGCCACCTGGACATGGCCG
  ACCTAGAAGCCAAGCTGCAGGAGGCCCAGAAGCATCGGCTGCGCCTGGTGGCCACTGATG
  GNGCCTTTTCCATGGATGGCGACATCGCACCCCTGCANGAGATCTGCTGCCTCGCCTCTA
  GATATGGTGCCCTGGTCTTCATGGATGAATGCCATGCCACTGGCTTCCTGGGGCCCACAG
  GACNGGGCACAGATGAGCTGCTGGGTGTGATGGACCAGGTCACCATCATCAACTCCACCC
  TGGGGAAANGCCCCTGGTGGAGCATCAAGGGGCTACACGACAGGGCCTGGGCCCCTGTTG
  TCCTGCTGCGCAGCGCGCCCGGCATACCTCTTCTCAAAGTCTGCAACTGCTG
  
• Restriction Sites: Please inquire
• ACCN: NM_014291
• ORF Size: 1260 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_014291.2, NP_055106.1
• RefSeq Size: 1470
• RefSeq ORF: 1260
• Locus ID: 23464
• Domains: aminotran_1_2
• Protein Pathways: Glycine, serine and threonine metabolism
• Gene Summary: The degradation of L-threonine to glycine consists of a two-step biochemical pathway involving the enzymes L-threonine dehydrogenase and 2-amino-3-ketobutyrate coenzyme A ligase. L-Threonine is first converted into 2-amino-3-ketobutyrate by L-threonine dehydrogenase. This gene encodes the second enzyme in this pathway, which then catalyzes the reaction between 2-amino-3-ketobutyrate and coenzyme A to form glycine and acetyl-CoA. The encoded enzyme is considered a class II pyridoxal-phosphate-dependent aminotransferase. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 14. [provided by RefSeq, Jan 2010]
  Transcript Variant: This variant (2) differs in the 3' UTR and uses an alternate splice site in the coding region, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.