GCAT (NM_014291) Human Untagged Clone

CAT#: SC319630

GCAT (untagged)-Human glycine C-acetyltransferase (GCAT), nuclear gene encoding mitochondrial protein, transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GCAT
• Synonyms: KBL
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_014291.2 GCTCGGGCGAGGTAGGAGCGATGTGGCCTGGGAACGCCTGGCGCGCCGCACTCTTCTGGG
  TGCCCCGCGGCCGCCGCGCACAGTCAGCGCTGGCCCAGCTGCGTGGCATTCTGGAGGGGG
  AGCTGGAAGGCATCTGCGGAGCTGGCACTTGGAAGAGTGAGCGGGTCATCACGTCCCGTC
  AGGGGCCGCACATCCGCGTGGACGGCGTCTCCGGAGGAATCCTTAACTTCTGTGCCAACA
  ACTACCTGGGCCTGAGCAGCCACCCTGAGGTGATCCAGGCAGGTCTGCAGGCTCTGGAGG
  AGTTTGGAGCTGGCCTCAGCTCTGTCCGCTTTATCTGTGGAACCCAGAGCATCCACAAGA
  ATCTAGAAGCAAAAATAGCCCGCTTCCACCAGCGGGAGGATGCCATCCTCTATCCCAGCT
  GTTATGACGCCAACGCCGGCCTCTTTGAGGCCCTGCTGACCCCAGAGGACGCAGTCCTGT
  CGGACGAGCTGAACCATGCCTCCATCATCGACGGCATCCGGCTGTGCAAGGCCCACAAGT
  ACCGCTATCGCCACCTGGACATGGCCGACCTAGAAGCCAAGCTGCAGGAGGCCCAGAAGC
  ATCGGCTGCGCCTGGTGGCCACTGATGGGGCCTTTTCCATGGATGGCGACATCGCACCCC
  TGCAGGAGATCTGCTGCCTCGCCTCTAGATATGGTGCCCTGGTCTTCATGGATGAATGCC
  ATGCCACTGGCTTCCTGGGGCCCACAGGACGGGGCACAGATGAGCTGCTGGGTGTGATGG
  ACCAGGTCACCATCATCAACTCCACCCTGGGGAAGGCCCTGGGTGGAGCATCAGGGGGCT
  ACACGACAGGGCCTGGGCCCCTGGTGTCCCTGCTGCGGCAGCGCGCCCGGCCATACCTCT
  TCTCCAACAGTCTGCCACCTGCTGTCGTTGGCTGCGCCTCCAAGGCCCTAGATCTGCTGA
  TGGGGAGTAACACCATTGTCCAGTCTATGGCTGCCAAGACCCAGAGGTTCCGTAGTAAGA
  TGGAAGCTGCTGGCTTCACTATCTCGGGAGCCAGTCACCCCATCTGCCCTGTGATGCTGG
  GTGATGCCCGGCTGGCCTCTCGCATGGCGGATGACATGCTGAAGAGAGGCATCTTTGTCA
  TCGGGTTCAGCTACCCCGTGGTCCCCAAGGGCAAGGCCTGGATCCGGGTACAGATCTCAG
  CAGTGCATAGCGAGGAAGACATTGACCGCTGCGTGGAGGCCTTCGTGGAAGTGGGGCGAC
  TGCACGGGGCACTGCCCTGAGCTCTGGGTAAGGACGAGAAGAGCCAAGGTCCGCCTGCTG
  CCACAGGGTCAAAGGAGGTTTTCGATCAGCCCAGACCAGAGGCTCTGAGCCCTGAACCAA
  AGTCCCAGAGCTGGGCTGGGACGTGACCTGTGCTGAGGGCTGTGAGAATGTGAAACAACA
  GTGTGAAAATTGGCTGTGCCAAAAAAAAAAAAAAAAAAAAAA
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_014291.2 unedited
  GATCGCTGTCGTCTCTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGGCACGAGGGC
  TCGGGCGAGGTAGGAGCGATGTGGCCTGGGAACGCCTGGCGCGCCGCACTCTTCTGGGTG
  CCCCGCGGCCGCCGCGCACAGTCAGCGCTGGCCCAGCTGCGTGGCATTCTGGAGGGGGAG
  CTGGAAGGCATCTGCGGAGCTGGCACTTGGAAGAGTGAGCGGGTCATCACGTCCCGTCAG
  GGGCCGCACATCCGCGTGGACGGCGTCTCCGGAGGAATCCTTAACTTCTGTGCCAACAAC
  TACCTGGGCCTGAGCAGCCACCCTGAGGTGATCCAGGCAGGTCTGCAGGCTCTGGAGGAG
  TTTGGAGCTGGCCTCAGCTCTGTCCGCTTTATCTGTGGAACCCAGAGCATCCACAAGAAT
  CTAGAAGCAAAAATAGCCCGCTTCCACCAGCGGGAGGATGCCATCCTCTATCCCAGCTGT
  TATGACGCCAACGCCGGCCTCTTTGAGGCCCTGCTGACCCCAGAGGACGCAGTCCTGTCG
  GACGAGCTGAACCATGCCTCCATCATCGACGGCATCCGGCTGTGCAAGGCCCACAAGTAC
  CGCTATCGCCACCTGGACATGGCCGACCTAGAAGCCAAGCTGCAGGAGGCCCAGAAGCAT
  CGGCTGCGCCTGGTGGCCACTGATGGGGCCTTTTCCATGGATGGCGACATCGCACCCCTG
  CAGGAGATCTGCTGCCTCGCCTCTAGATATGGTGCCCTGGTCTTCATGGGATGAATGCCA
  TGCCACTGGCTTCCTGGGGCCCACAGGACGGGGCACAGATGAGCTGCTGGGTGGTGATGG
  ACCAGGTCACCATCATCACTCCACCCTGGGGAGGCCTGGGGTGGGGAGCATCAAGGGGGC
  TACACGACCAGGTCTGGCCCTTGGGTGTCTTGCCTGCGCAAGCGGGCCGCCATACTCTTC
  TCCACAGTCTGGCACTGGCTGTGTCC
• Restriction Sites: Please inquire
• ACCN: NM_014291
• ORF Size: 1260 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_014291.2, NP_055106.1
• RefSeq Size: 1470
• RefSeq ORF: 1260
• Locus ID: 23464
• Domains: aminotran_1_2
• Protein Pathways: Glycine, serine and threonine metabolism
• Gene Summary: The degradation of L-threonine to glycine consists of a two-step biochemical pathway involving the enzymes L-threonine dehydrogenase and 2-amino-3-ketobutyrate coenzyme A ligase. L-Threonine is first converted into 2-amino-3-ketobutyrate by L-threonine dehydrogenase. This gene encodes the second enzyme in this pathway, which then catalyzes the reaction between 2-amino-3-ketobutyrate and coenzyme A to form glycine and acetyl-CoA. The encoded enzyme is considered a class II pyridoxal-phosphate-dependent aminotransferase. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 14. [provided by RefSeq, Jan 2010]
  Transcript Variant: This variant (2) differs in the 3' UTR and uses an alternate splice site in the coding region, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.