p57 Kip2 (CDKN1C) (NM_000076) Human Untagged Clone

CAT#: SC127869

CDKN1C (untagged)-Human cyclin-dependent kinase inhibitor 1C (p57, Kip2) (CDKN1C), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CDKN1C
• Synonyms: BWCR; BWS; KIP2; p57; p57Kip2; WBS
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_000076, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTCCGACGCGTCCCTCCGCAGCACATCCACGATGGAGCGTCTTGTCGCCCGTGGGACCTTCCCAGTAC
  TAGTGCGCACCAGCGCCTGCCGCAGCCTCTTCGGGCCGGTGGACCACGAGGAGCTGAGCCGCGAGCTGCA
  GGCCCGCCTGGCCGAGCTGAACGCCGAGGACCAGAACCGCTGGGATTACGACTTCCAGCAGGACATGCCG
  CTGCGGGGCCCTGGACGCCTGCAGTGGACCGAAGTGGACAGCGACTCGGTGCCCGCGTTCTACCGCGAGA
  CGGTGCAGGTGGGGCGCTGCCGCCTGCTGCTGGCGCCGCGGCCCGTCGCGGTCGCGGTGGCTGTCAGCCC
  GCCCCTCGAGCCGGCCGCTGAGTCCCTCGACGGCCTCGAGGAGGCGCCGGAGCAGCTGCCTAGTGTCCCG
  GTCCCGGCCCCGGCGTCCACCCCGCCCCCAGTCCCGGTCCTGGCTCCAGCCCCGGCCCCGGCTCCGGCTC
  CGGTCGCGGCTCCGGTCGCGGCTCCGGTCGCGGTCGCGGTCCTGGCCCCGGCCCCGGCCCCGGCTCCGGC
  TCCGGCTCCGGCCCCGGCTCCAGTCGCGGCCCCGGCCCCAGCCCCGGCCCCGGCCCCGGCCCCGGCCCCC
  GCCCCGGCCCCGGCCCCGGACGCGGCGCCTCAAGAGAGCGCCGAGCAGGGCGCGAACCAGGGGCAGCGCG
  GCCAGGAGCCTCTCGCTGACCAGCTGCACTCGGGGATTTCGGGACGTCCCGCGGCCGGCACCGCGGCCGC
  CAGCGCCAACGGCGCGGCGATCAAGAAGCTGTCCGGGCCTCTGATCTCCGATTTCTTCGCCAAGCGCAAG
  AGATCAGCGCCTGAGAAGTCGTCGGGCGATGTCCCCGCGCCGTGTCCCTCTCCAAGCGCCGCCCCTGGCG
  TGGGCTCGGTGGAGCAGACCCCGCGCAAGAGGCTGCGGTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000076 unedited
  CCCCCGTCGGATTTTGTATACGACTTCACTATAGGCGGCCGCGCATTCGGCACGAGCTCG
  AGGGTGCCGGCCAGGCCTGAGCGAGCGAGCTAGCCAGCAGGCATCGAGGGGGCGCGGCTG
  CCGTCCGGACGAGACAGGCGAACCCGACGCAGAAGAGTCCACCACCGGACAGCCAGGTAG
  CCGCCGCGTCCCTCGCACACGCAGAGTCGGGCGGCGCGGGGTCTCCCTTGCGCCCGGCCT
  CCGCCCTCTCCTCCTCTCCTTTCCCCTTCTTCTCGCTGTCCTCTCCTCTCTCGCTGCCCG
  CGTTTGCGCAGCCCCGGGCCATGTCCGACGCGTCCCTCCGCAGCACATCCACGATGGAGC
  GTCTTGTCGCCCGTGGGACCTTCCCAGTACTAGTGCGCACCAGCGCCTGCCGCAGCCTCT
  TCGGGCCGGTGGACCACGAGGAGCTGAGCCGCGAGCTGCAGGCCCGCCTGGTCGAGCTGA
  ACGCCGAGGACCCGAACCGCTGGGATTACGACTTCCAGCAGGAAATGCCGCTGCGGGGCC
  CTGGACGCCTGCAGTGGACCGAAATGGACAGCGACTCGGTGCCTGCGGTCTACCCCGAGA
  CCGTGCAAGTGGGGCGCTGCCGCCCTCCGCTAATGCCCCCCCCCTTCTCGTTCTTGGGGG
  CTCCCCCCCCGCTCCTTGATCCCGCCCCTGAATGCTTTGACCGCCTTTTGGAGGCGCCCG
  GAGCATCTGCCCAATGTTCCTTGTTCTGGCACTGCCTCCACACTGGCCGTGATCACGGGT
  GTGCCTAATTTAAGGGCACGGTCCGGTCCGGACGCGGCGCCGGCGCCGCCCAAGAATTGT
  ATACGGGACCGGGTCAGGCTGCTCAACCGGATCATCCCTATTATGCGACCCG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000076 unedited
  GCACTGNNNATTCCCGGCCAGGAGTGGCACTGGGGAGGGGTCACAGGGATGCCACCCGGG
  ATCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTT
  TTTTTGCACTGAGTTTCAGCAGAGATTAAACATTTTATATAAATGACTCTTAAAGCTTTA
  CACCTTGGGACCAGTGTACCTTCTCGTGCAGAATACATTTAGATATAAAAAGACGTTATT
  AATACATTGCACAGTTTTCAAAATTTAAAAACAAAACCGAACGCTGCTCTGCGGCAGCCG
  CCGCCGGTTGCTGCTACATGAACGGTCCCAGCCGAGGCCCAGCGCCCTTCCAACGTCCGC
  TGCCCCGGCAGGTTCCCTCGGGGCTCTTTGGGCTCTAAATTGGCTCACCGCAGCCTCTTG
  CGCGGGGTCTGCTCCACCGAGCCCACGCCAGGGGCGGCGCTTGGAGAGGGACACGGCGCG
  GGGACATCGCCCGACGACTTCTCAGGCGCTGATCTCTTGCGCTTGGCGAAGAAATCGGAG
  ATCAGAGGCCCGGACAGCTTCTTGATCGCCGCGCCGTTGGCGCTGGCGGCCGCGGTGCCG
  GCCGCGGGACGTCCCGAAATCCCCGAGTGCAGCTGGTCAACGAGAGGCTCCTGGCCGCGC
  TGCCCCTGGTTCGCGCCCTGGTCGGCGCTCTTTTTGAGCGCCGCGTCCGGGGCCG
  
• Restriction Sites: Please inquire
• ACCN: NM_000076
• Insert Size: 1700 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000076.1, NP_000067.1
• RefSeq Size: 1511 bp
• RefSeq ORF: 951 bp
• Locus ID: 1028
• Cytogenetics: 11p15.4
• Domains: CDI
• Protein Families: Druggable Genome
• Protein Pathways: Cell cycle
• Gene Summary: 'This gene is imprinted, with preferential expression of the maternal allele. The encoded protein is a tight-binding, strong inhibitor of several G1 cyclin/Cdk complexes and a negative regulator of cell proliferation. Mutations in this gene are implicated in sporadic cancers and Beckwith-Wiedemann syndorome, suggesting that this gene is a tumor suppressor candidate. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Oct 2010]'
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Variants 1 and 3 both encode isoform a.