GLP1 (GCG) (NM_002054) Human Untagged Clone

CAT#: SC127874

GCG (untagged)-Human glucagon (GCG)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GCG
• Synonyms: GLP-1; GLP1; GLP2; GRPP
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC127874 sequence for NM_002054 edited (data generated by NextGen Sequencing)
  ATGAAAAGCATTTACTTTGTGGCTGGATTATTTGTAATGCTGGTACAAGGCAGCTGGCAA
  CGTTCCCTTCAAGACACAGAGGAGAAATCCAGATCATTCTCAGCTTCCCAGGCAGACCCA
  CTCAGTGATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAGGGCACATTCACCAGT
  GACTACAGCAAGTATCTGGACTCCAGGCGTGCCCAAGATTTTGTGCAGTGGTTGATGAAT
  ACCAAGAGGAACAGGAATAACATTGCCAAACGTCACGATGAATTTGAGAGACATGCTGAA
  GGGACCTTTACCAGTGATGTAAGTTCTTATTTGGAAGGCCAAGCTGCCAAGGAATTCATT
  GCTTGGCTGGTGAAAGGCCGAGGAAGGCGAGATTTCCCAGAAGAGGTCGCCATTGTTGAA
  GAACTTGGCCGCAGACATGCTGATGGTTCTTTCTCTGATGAGATGAACACCATTCTTGAT
  AATCTTGCCGCCAGGGACTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA
  TAA
  
  Clone variation with respect to NM_002054.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002054 unedited
  ATAGGGCGGCCGCGAATTCAGATCTAAGAGGGCTTGCTCTCTCTTCACCTGCTCTGTTCT
  ACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCTGGATTATTT
  GTAATGCTGGTACAAGGCAGCTGGCAACGTTCCCTTCAAGACACAGAGGAGAAATCCAGA
  TCATTCTCAGCTTCCCAGGCAGACCCACTCAGTGATCCTGATCAGATGAACGAGGACAAG
  CGCCATTCACAGGGCACATTCACCAGTGACTACAGCAAGTATCTGGACTCCAGGCGTGCC
  CAAGATTTTGTGCAGTGGTTGATGAATACCAAGAGGAACAGGAATAACATTGCCAAACGT
  CACGATGAATTTGAGAGACATGCTGAAGGGACCTTTACCAGTGATGTAAGTTCTTATTTG
  GAAGGCCAAGCTGCCAAGGAATTCATTGCTTGGCTGGTGAAAGGCCGAGGAAGGCGAAGA
  TTCCCAAGAAGAAGTCCCCAATTGTGGAAAACCTTGCCGCGCAACAGGCGGAGGGTCCTT
  CCTCGGAGAGAGGAACACCATTCTTGATAATCTTGCCGCCAGGCACTTTATAAACTGTTT
  GATTCAGCCCAAATTCACTGACAGGAAATAACTATATCACTATTCAAGATCATCTTCACA
  ACATCACCTGCTAGCCN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002054 unedited
  GGATGCAACTTCCAGGCCAGGAGAGGCACTGGGGAGGGGTCACAGGGATGCCACCCGGGA
  TCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAAGCAATGTGGCCTCAGAATA
  CACCTCTTAAATTTACAGGACTTAACATTTCAAACATCCCACGTGGCTAGCAGGTGATGT
  TGTGAAGATGATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATC
  AACCAGTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTCATCTCATCAGAG
  AAAGAACCATCAGCATGTCTGCGGCCAAGTTCTTCAACAATGGCGACCTCTTCTGGGAAA
  TCTCGCCTTCCTCGGCCTTTCACCAGCCAAGCAATGAATTCCTTGGCAGCTTGGCCTTCC
  AAATAAGAACTTACATCACTGGTAAAGGTCCCTTCAGCATGTCTCTCAAATTCATCGTGA
  CGTTTGGCAATGTTATTCCTGTTCCTCTTGGTATTCATCAACCACTGCACAAAATCTTGG
  GCACGCCTGGAGTCCAGATACTTGCTGTAGTCACTGGTGAATGTGCCCTGTGAATGGCGC
  TTGTCCTCGTTCATCTGATCAGGATCACTGAGTGGGTCTGCCTGGGAAGCTGAGAATGAT
  CTGGNATTTCTCTCTGTGTCCTGAAGGNAACGTTGCCAGCTGCCTTGTACCAGCATTACA
  AATAATCCAGCCACAAAGTAAATGCTTTTCATTTCTGCCTGCCTCCGGTATGTGCCGTAA
  AACAAAGCAGGTGAAAAGAGACAGACCTCTTAAATCGATTCGCGGCGGCCTATATGGAGT
  CGATACCAAATTCGACGGTTACTAACCAGCTCGCTAATAGACCTCCC
  
• Restriction Sites: Please inquire
• ACCN: NM_002054
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002054.2, NP_002045.1
• RefSeq Size: 1128 bp
• RefSeq ORF: 543 bp
• Locus ID: 2641
• Cytogenetics: 2q24.2
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein
• Gene Summary: 'The protein encoded by this gene is actually a preproprotein that is cleaved into four distinct mature peptides. One of these, glucagon, is a pancreatic hormone that counteracts the glucose-lowering action of insulin by stimulating glycogenolysis and gluconeogenesis. Glucagon is a ligand for a specific G-protein linked receptor whose signalling pathway controls cell proliferation. Two of the other peptides are secreted from gut endocrine cells and promote nutrient absorption through distinct mechanisms. Finally, the fourth peptide is similar to glicentin, an active enteroglucagon. [provided by RefSeq, Jul 2008]'