PPM1A (NM_177952) Human Untagged Clone

CAT#: SC127980

PPM1A (untagged)-Human protein phosphatase, Mg2+/Mn2+ dependent, 1A (PPM1A), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PPM1A
• Synonyms: PP2C-ALPHA; PP2CA; PP2Calpha
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_177952, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTTCTGTTCTGGGAGAAAATGGGTAGCAGAAGCTACAATTTGTACTAAGCTAATGAAAAGAGAGAAAA
  GAAGAATGGGGAAGAGAAGGGCAAAGAAGGCAAAAAGAGAAGAGAAGAAAAAGGGAGGAGAGAGAAGGAG
  AAATGAGAAAAGAGGAAACCAAATGAAGAGGATGTGTGAGAGAAAAAAATATGAAACAGACCTAGAGGAT
  CAAGACATAATGGGAGCATTTTTAGACAAGCCAAAGATGGAAAAGCATAATGCCCAGGGGCAGGGTAATG
  GGTTGCGATATGGGCTAAGCAGCATGCAAGGCTGGCGTGTTGAAATGGAGGATGCACATACGGCTGTGAT
  CGGTTTGCCAAGTGGACTTGAATCGTGGTCATTCTTTGCTGTGTATGATGGGCATGCTGGTTCTCAGGTT
  GCCAAATACTGCTGTGAGCATTTGTTAGATCACATCACCAATAACCAGGATTTTAAAGGGTCTGCAGGAG
  CACCTTCTGTGGAAAATGTAAAGAATGGAATCAGAACAGGTTTTCTGGAGATTGATGAACACATGAGAGT
  TATGTCAGAGAAGAAACATGGTGCAGATAGAAGTGGGTCAACAGCTGTAGGTGTCTTAATTTCTCCCCAA
  CATACTTATTTCATTAACTGTGGAGACTCAAGAGGTTTACTTTGTAGGAACAGGAAAGTTCATTTCTTCA
  CACAAGATCACAAACCAAGTAATCCGCTGGAGAAAGAACGAATTCAGAATGCAGGTGGCTCTGTAATGAT
  TCAGCGTGTGAATGGCTCTCTGGCTGTATCGAGGGCCCTTGGGGATTTTGATTACAAATGTGTCCATGGA
  AAAGGTCCTACTGAGCAGCTTGTCTCACCAGAGCCTGAAGTCCATGATATTGAAAGATCTGAAGAAGATG
  ATCAGTTCATTATCCTTGCATGTGATGGTATCTGGGATGTTATGGGAAATGAAGAGCTCTGTGATTTTGT
  AAGATCCAGACTTGAAGTCACTGATGACCTTGAGAAAGTTTGCAATGAAGTAGTCGACACCTGTTTGTAT
  AAGGGAAGTCGAGACAACATGAGTGTGATTTTGATCTGTTTTCCAAATGCACCCAAAGTATCGCCAGAAG
  CAGTGAAGAAGGAGGCAGAGTTGGACAAGTACCTGGAATGCAGAGTAGAAGAAATCATAAAGAAGCAGGG
  GGAAGGCGTCCCCGACTTAGTCCATGTGATGCGCACATTAGCGAGTGAGAACATCCCCAGCCTCCCACCA
  GGGGGTGAATTGGCAAGCAAGAGGAATGTTATTGAAGCCGTTTACAATAGACTGAATCCTTACAAAAATG
  ACGACACTGACTCTACATCAACAGATGATATGTGGTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_177952 unedited
  TCTCTGCCTCCCTCTCCAACGCCCGGATGATCTGAGCCGCGAGGGCGCCGACAGCCGGGG
  GCCCGGACGCAGCCCGGCTCCTCCCCTCCTCCGCCCCTTCCCCAGCCTGACCTGGCCCGC
  CGCTGCAGCGGTGACCCCTCCCCCGGCTGCCGCCGTCGCCGCCGCGGTGACCCCCTCCCC
  GGCTGCCGCCGCCGCCGCCTCGGCCGACCAGGGACCTGCCCGCCTGCGGCTGCTCCGGAC
  CTAGAGGATCAAGACATAATGGGAGCATTTTTAGACAAGCCAAAGATGGAAAAGCATAAT
  GCCCAGGGGCAGGGTAATGGGTTGCGATATGGGCTAAGCAGCATGCAAGGCTGGCGTGTT
  GAAATGGAGGATGCACATACGGCTGTGATCGGTTTGCCAAGTGGACTTGAATCGTGGTCA
  TTCTTTGCTGTGTATGATGGGCATGCTGGTTCTCAGGTTGCCAAATACTGCTGTGAGCAT
  TTGTTAGATCACATCACCAATAACCAGGATTNTAAAGGGTCTGCAGGAGCACCTTCTGTG
  GAAAATGTAAAGAATGGAATCAGAACAGGTTNTCTGGGAGATTGATGAACCATGAGAGTT
  ATGTCAGAGAGAAACATGGTGCGATAGAAGTGGGTAACAGCGGAAGGTGTCTAANTTTCT
  CCCCACATACTTATTCATTACTGTGAACNNAAGAGGNTACTTGTAGACCCGGAAAGTCAT
  TNCTCCACAGATCACAACCAAGTATCGCTGAGAANGACGATCNNATGCAGTGGCTTGAAT
  GATCACGGGGGATGCTTCTGTGNNATCAGGCCTTGGGGATTTGATAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' genomic read for NM_177952 unedited
  CATAGATAATGGCGATGGCAACTTCCAGGNCCAGNANGAGCACTGGGGNAGGGTCACAGG
  GCATGCCACCCGGGCACTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGA
  GTTTTTTTTTTTTTTTTTTTGGTTTGCCAAGTTCCTTTATTGTGTAATCTTTAACATCTA
  GTATATTTGTTCTACATGGTTCTAATGCTGGAAATCTGCTGTACAAAAATAAAGGGATTT
  GATGTTTACCTAAACTGCACAATTTCACAATCTAAAACTAATACACTTAACATACAGTCT
  CCGCTAAGCCAATACAATTATTTGGATAGATAACTAGATACAATAGAAATAAACACTGAT
  GATAAATTGAATCAATAGTTTCAGGTCTAAAAAACATTACCTTGAATCATCTATATTTGA
  TGTATCTTACATCTCTTTTGTTAAAATATAAATTAGGATATTTTAACTGTGAAGAAAATT
  ACAGGAAAACAAATTCTTGTAAAAAAATAATGTAAAGCTTTAAAAACAGTTAATATCTCC
  TGTTCCACGTGGGACAACGCATTTAAACTACCTGACCATTCATACTATACTAGCCTTGCT
  TCTGTCTGCTTTCAAAAAAGAAAAATAAATGAATTTTAAGAAATATTCCTTTAATTTCTG
  TTGACCTATACGTTTTACTTAGAATTGTAAAAAGACTGAACGCTTTTTCTAAAGAATATT
  TATATATAGGAAGATCCATGTTTCACATACATATACACAAACCCATTCTGCTCCTTTTCC
  CTCCCCTCCACCAAGTTTCAGGGGATCCTTCCGTCATCTCCAATTTTCCACTTTTAACCT
  NAGGTGGTTCCGTGCCCAAAATTTTGGAAATTTTCCCCCAAAGTTTATTTTA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_177952
• Insert Size: 5000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_177952.1, NP_808821.1
• RefSeq Size: 8075 bp
• RefSeq ORF: 1149 bp
• Locus ID: 5494
• Cytogenetics: 14q23.1
• Protein Families: Druggable Genome, Phosphatase
• Protein Pathways: MAPK signaling pathway
• Gene Summary: 'The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase dephosphorylates, and negatively regulates the activities of, MAP kinases and MAP kinase kinases. It has been shown to inhibit the activation of p38 and JNK kinase cascades induced by environmental stresses. This phosphatase can also dephosphorylate cyclin-dependent kinases, and thus may be involved in cell cycle control. Overexpression of this phosphatase is reported to activate the expression of the tumor suppressor gene TP53/p53, which leads to G2/M cell cycle arrest and apoptosis. Three alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (3) has an alternate 5' exon, as compared to variant 1. It encodes isoform 3, which has a distinct and longer N-terminus, as compared to isoform 1.