Protein Phosphatase 1 beta (PPP1CB) (NM_206876) Human Untagged Clone

CAT#: SC127982

PPP1CB (untagged)-Human protein phosphatase 1, catalytic subunit, beta isozyme (PPP1CB), transcript variant 3


  "NM_206876" in other vectors (6)

Reconstitution Protocol

USD 310.00

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol PPP1CB
Synonyms HEL-S-80p; NSLH2; PP-1B; PP1B; PP1beta; PPP1CD
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for NM_206876, the custom clone sequence may differ by one or more nucleotides


ATGGCGGACGGGGAGCTGAACGTGGACAGCCTCATCACCCGGCTGCTGGAGGTACGAGGATGTCGTCCAG
GAAAGATTGTGCAGATGACTGAAGCAGAAGTTCGAGGCTTATGTATCAAGTCTCGGGAGATCTTTCTCAG
CCAGCCTATTCTTTTGGAATTGGAAGCACCGCTGAAAATTTGTGGAGATATTCATGGACAATATACAGAT
TTACTGAGATTATTTGAATATGGAGGTTTCCCACCAGAAGCCAACTATCTTTTCTTAGGAGATTATGTGG
ACAGAGGAAAGCAGTCTTTGGAAACCATTTGTTTGCTATTGGCTTATAAAATCAAATATCCAGAGAACTT
CTTTCTCTTAAGAGGAAACCATGAGTGTGCTAGCATCAATCGCATTTATGGATTCTATGATGAATGCAAA
CGAAGATTTAATATTAAATTGTGGAAGACCTTCACTGATTGTTTTAACTGTCTGCCTATAGCAGCCATTG
TGGATGAGAAGATCTTCTGTTGTCATGGAGGATTGTCACCAGACCTGCAATCTATGGAGCAGATTCGGAG
AATTATGAGACCTACTGATGTCCCTGATACAGGTTTGCTCTGTGATTTGCTATGGTCTGATCCAGATAAG
GATGTGCAAGGCTGGGGAGAAAATGATCGTGGTGTTTCCTTTACTTTTGGAGCTGATGTAGTCAGTAAAT
TTCTGAATCGTCATGATTTAGATTTGATTTGTCGAGCTCATCAGGTGGTGGAAGATGGATATGAATTTTT
TGCTAAACGACAGTTGGTAACCTTATTTTCAGCCCCAAATTACTGTGGCGAGTTTGATAATGCTGGTGGA
ATGATGAGTGTGGATGAAACTTTGATGTGTTCATTTCAGATATTGAAACCATCTGAAAAGAAAGCTAAAT
ACCAGTATGGTGGACTGAATTCTGGACGTCCTGTCACTCCACCTCGAACAGCTAATCCGCCGAAGAAAAG
GTGA


>OriGene 5' read for NM_206876 unedited
GTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCGGCGCGCAGGGACGTG
CGGAGTGAGTGGCGCTGCGGGTGGGGCCGTCGGCGGCGCTGGTGAGAGAACGCCGAGCCG
TCGCCGCAGCCTCCGCCGCCGAGAAGCCCTTGTTCCCGCTGCTGGGAAGGAGAGTCTGTG
CCGACAAGATGGCGGACGGGGAGCTGAACGTGGACAGCCTCATCACCCGGCTGCTGGAGG
TACGAGGATGTCGTCCAGGAAAGATTGTGCAGATGACTGAAGCAGAAGTTCGAGGCTTAT
GTATCAAGTCTCGGGAGATCTTTCTCAGCCAGCCTATTCTTTTGGAATTGGAAGCACCGC
TGAAAATTTGTGGAGATATTCATGGACAGTATACAGATTTACTGAGATTATTTGAATATG
GAGGTTTCCCACCAGAAGCCAACTATCTTTTCTTAGGAGATTATGTGGACAGAGGAAAGC
AGTCTTTGGAAACCATTTGTTTGCTATTGGCTTATAAAATCAAATATCCAGAGAACTTCT
TTCTCTTAAGAGGAAACCATGAGTGTGCTAGCATCAATCGCATTTATGGATTCTATGATG
AATGCAAACGAAGATTTAATATTAAATTGTGGAAGACCTTCACTGATTGTTTTAACTGTC
TGCCTATAGCAGCCATTGTGGATGAGAAGATCTTTCTGTGTCATGGAGGATTGTCACCAG
ACCTGNCATCTATGGAGCAGNATTCGAGAATATGAGACCTACTGATGTCCCTGATACAGG
NTTGCTCTGTGATTTGCTATGGTCTGATCCANATNAGGATGTGCANGGCTGGGGAGAAAT
GATCGTGGTGTTTCCTTTACTTTGNAGCTGATGTATCAGTAAATTTCTGATCGTCATGAT
TA
>Reverse primer walk for NM_206876 unedited
TTTGCGCGCGATTGGGTTAGNCACTCTTACTAATGANAAACTACTTTAAAAGTGCTTTAA
AACAAAACGGATTAGAAAAAAAGACATATGCATTTTAAAGAACTTTTGCACTGTGCTGAA
AATGAAATATCTCTTGCAGTAAGTAATACAATGTGCCAATCCATAAAATGTAAGTTAACA
GGTTTCTCAATTTAACATTTTCAAAGACGATATTAAGGTCAAAAATGTCACATCTATGGT
AGCACATATAGAAGGGCATTATAGATATGATGTCCATCCCTATGAAGCTTACAAATTTGA
CAATGCTCAATAAAAGCTTTTAGAATCACATCCAATAGTGTCACAGTCAAAGAATCACAA
ATCTGTGTCCTGTAAGTTCTCTCCTTCTAAAATCTTCATGTTAACTTTTCTACTCATTGC
CATTTGTTAATACTAACCCTTAAAAAAAAGAAATAGGAAAAGAAAGGCTGCAATAAACTT
GATCAGGTACAGAAATTATTGCTACTATACTTCACTACAAAGCTTGTCTATACAGTATCA
TATGACCTTTGATTATGAAAAAATCAGAATAAAAATCTTTAGTGACATAAGCCTTACAAT
CGTATACAACATTCACATGGCAATATTAGACAGTTAAGCACCCAATACCCATAGTTGACA
AAATGTCCCACTGACCAGCATTCATTTAAATACATCCTTCGTATAGAGGGAGGAAAAAGA
GACAGTGTCAGCTTTCCAAGGCTTGTCAAAAAAGGATTCTCATGTACTGTGGAATCTAAA
AACAAACAAAAACTCATAAAAAAATAAAAAATAAAAGTAAGGAAAAAGGAACAAGAAACC
AATGCCGGTG
Restriction Sites NotI-NotI     
ACCN NM_206876
Insert Size 3000 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_206876.1, NP_996759.1
RefSeq Size 4786 bp
RefSeq ORF 984 bp
Locus ID 5500
Cytogenetics 2p23.2
Protein Families Druggable Genome, Phosphatase
Protein Pathways Focal adhesion, Insulin signaling pathway, Long-term potentiation, Oocyte meiosis, Regulation of actin cytoskeleton, Vascular smooth muscle contraction
Gene Summary 'The protein encoded by this gene is one of the three catalytic subunits of protein phosphatase 1 (PP1). PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Mouse studies suggest that PP1 functions as a suppressor of learning and memory. Two alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]'
Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. Both variants encode the same isoform (1).

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