Protein Phosphatase 1 beta (PPP1CB) (NM_206876) Human Untagged Clone

CAT#: SC127982

PPP1CB (untagged)-Human protein phosphatase 1, catalytic subunit, beta isozyme (PPP1CB), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PPP1CB
• Synonyms: HEL-S-80p; NSLH2; PP-1B; PP1B; PP1beta; PPP1CD
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_206876, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCGGACGGGGAGCTGAACGTGGACAGCCTCATCACCCGGCTGCTGGAGGTACGAGGATGTCGTCCAG
  GAAAGATTGTGCAGATGACTGAAGCAGAAGTTCGAGGCTTATGTATCAAGTCTCGGGAGATCTTTCTCAG
  CCAGCCTATTCTTTTGGAATTGGAAGCACCGCTGAAAATTTGTGGAGATATTCATGGACAATATACAGAT
  TTACTGAGATTATTTGAATATGGAGGTTTCCCACCAGAAGCCAACTATCTTTTCTTAGGAGATTATGTGG
  ACAGAGGAAAGCAGTCTTTGGAAACCATTTGTTTGCTATTGGCTTATAAAATCAAATATCCAGAGAACTT
  CTTTCTCTTAAGAGGAAACCATGAGTGTGCTAGCATCAATCGCATTTATGGATTCTATGATGAATGCAAA
  CGAAGATTTAATATTAAATTGTGGAAGACCTTCACTGATTGTTTTAACTGTCTGCCTATAGCAGCCATTG
  TGGATGAGAAGATCTTCTGTTGTCATGGAGGATTGTCACCAGACCTGCAATCTATGGAGCAGATTCGGAG
  AATTATGAGACCTACTGATGTCCCTGATACAGGTTTGCTCTGTGATTTGCTATGGTCTGATCCAGATAAG
  GATGTGCAAGGCTGGGGAGAAAATGATCGTGGTGTTTCCTTTACTTTTGGAGCTGATGTAGTCAGTAAAT
  TTCTGAATCGTCATGATTTAGATTTGATTTGTCGAGCTCATCAGGTGGTGGAAGATGGATATGAATTTTT
  TGCTAAACGACAGTTGGTAACCTTATTTTCAGCCCCAAATTACTGTGGCGAGTTTGATAATGCTGGTGGA
  ATGATGAGTGTGGATGAAACTTTGATGTGTTCATTTCAGATATTGAAACCATCTGAAAAGAAAGCTAAAT
  ACCAGTATGGTGGACTGAATTCTGGACGTCCTGTCACTCCACCTCGAACAGCTAATCCGCCGAAGAAAAG
  GTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_206876 unedited
  GTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCGGCGCGCAGGGACGTG
  CGGAGTGAGTGGCGCTGCGGGTGGGGCCGTCGGCGGCGCTGGTGAGAGAACGCCGAGCCG
  TCGCCGCAGCCTCCGCCGCCGAGAAGCCCTTGTTCCCGCTGCTGGGAAGGAGAGTCTGTG
  CCGACAAGATGGCGGACGGGGAGCTGAACGTGGACAGCCTCATCACCCGGCTGCTGGAGG
  TACGAGGATGTCGTCCAGGAAAGATTGTGCAGATGACTGAAGCAGAAGTTCGAGGCTTAT
  GTATCAAGTCTCGGGAGATCTTTCTCAGCCAGCCTATTCTTTTGGAATTGGAAGCACCGC
  TGAAAATTTGTGGAGATATTCATGGACAGTATACAGATTTACTGAGATTATTTGAATATG
  GAGGTTTCCCACCAGAAGCCAACTATCTTTTCTTAGGAGATTATGTGGACAGAGGAAAGC
  AGTCTTTGGAAACCATTTGTTTGCTATTGGCTTATAAAATCAAATATCCAGAGAACTTCT
  TTCTCTTAAGAGGAAACCATGAGTGTGCTAGCATCAATCGCATTTATGGATTCTATGATG
  AATGCAAACGAAGATTTAATATTAAATTGTGGAAGACCTTCACTGATTGTTTTAACTGTC
  TGCCTATAGCAGCCATTGTGGATGAGAAGATCTTTCTGTGTCATGGAGGATTGTCACCAG
  ACCTGNCATCTATGGAGCAGNATTCGAGAATATGAGACCTACTGATGTCCCTGATACAGG
  NTTGCTCTGTGATTTGCTATGGTCTGATCCANATNAGGATGTGCANGGCTGGGGAGAAAT
  GATCGTGGTGTTTCCTTTACTTTGNAGCTGATGTATCAGTAAATTTCTGATCGTCATGAT
  TA
  
  3' Read Nucleotide Sequence

  >Reverse primer walk for NM_206876 unedited
  TTTGCGCGCGATTGGGTTAGNCACTCTTACTAATGANAAACTACTTTAAAAGTGCTTTAA
  AACAAAACGGATTAGAAAAAAAGACATATGCATTTTAAAGAACTTTTGCACTGTGCTGAA
  AATGAAATATCTCTTGCAGTAAGTAATACAATGTGCCAATCCATAAAATGTAAGTTAACA
  GGTTTCTCAATTTAACATTTTCAAAGACGATATTAAGGTCAAAAATGTCACATCTATGGT
  AGCACATATAGAAGGGCATTATAGATATGATGTCCATCCCTATGAAGCTTACAAATTTGA
  CAATGCTCAATAAAAGCTTTTAGAATCACATCCAATAGTGTCACAGTCAAAGAATCACAA
  ATCTGTGTCCTGTAAGTTCTCTCCTTCTAAAATCTTCATGTTAACTTTTCTACTCATTGC
  CATTTGTTAATACTAACCCTTAAAAAAAAGAAATAGGAAAAGAAAGGCTGCAATAAACTT
  GATCAGGTACAGAAATTATTGCTACTATACTTCACTACAAAGCTTGTCTATACAGTATCA
  TATGACCTTTGATTATGAAAAAATCAGAATAAAAATCTTTAGTGACATAAGCCTTACAAT
  CGTATACAACATTCACATGGCAATATTAGACAGTTAAGCACCCAATACCCATAGTTGACA
  AAATGTCCCACTGACCAGCATTCATTTAAATACATCCTTCGTATAGAGGGAGGAAAAAGA
  GACAGTGTCAGCTTTCCAAGGCTTGTCAAAAAAGGATTCTCATGTACTGTGGAATCTAAA
  AACAAACAAAAACTCATAAAAAAATAAAAAATAAAAGTAAGGAAAAAGGAACAAGAAACC
  AATGCCGGTG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_206876
• Insert Size: 3000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_206876.1, NP_996759.1
• RefSeq Size: 4786 bp
• RefSeq ORF: 984 bp
• Locus ID: 5500
• Cytogenetics: 2p23.2
• Protein Families: Druggable Genome, Phosphatase
• Protein Pathways: Focal adhesion, Insulin signaling pathway, Long-term potentiation, Oocyte meiosis, Regulation of actin cytoskeleton, Vascular smooth muscle contraction
• Gene Summary: 'The protein encoded by this gene is one of the three catalytic subunits of protein phosphatase 1 (PP1). PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Mouse studies suggest that PP1 functions as a suppressor of learning and memory. Two alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. Both variants encode the same isoform (1).