PPP2R1B (NM_002716) Human Untagged Clone

CAT#: SC127986

PPP2R1B (untagged)-Human protein phosphatase 2, regulatory subunit A, beta (PPP2R1B), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PPP2R1B
• Synonyms: PP2A-Abeta; PR65B
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_002716, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCGGGCGCATCAGAGCTCGGGACCGGCCCAGGAGCAGCGGGTGGAGATGGAGATGATTCGCTATACC
  CGATCGCGGTTTTAATCGACGAGCTCCGCAATGAAGACGTGCAGCTCCGACTCAACAGTATTAAGAAGTT
  ATCAACAATTGCCCTAGCACTTGGAGTAGAAAGGACCCGAAGTGAATTGTTGCCATTTCTTACAGATACA
  ATTTATGATGAAGATGAGGTACTATTAGCTCTTGCTGAGCAGCTGGGAAATTTCACTGGCCTAGTGGGAG
  GTCCTGACTTTGCCCACTGTCTGCTGCCTCCTTTGGAAAATCTGGCAACTGTGGAAGAGACTGTTGTTCG
  TGACAAGGCTGTGGAGTCCCTGAGACAGATCTCCCAGGAGCATACTCCTGTTGCTCTGGAAGCTTATTTT
  GTACCTCTGGTGAAACGCTTAGCAAGTGGGGATTGGTTCACCTCTCGCACATCTGCATGTGGTTTGTTCA
  GCGTTTGCTATCCCAGGGCATCAAATGCTGTTAAAGCAGAAATCAGACAGCAATTCCGTTCCTTGTGCTC
  AGATGACACACCAATGGTACGACGTGCTGCTGCTTCCAAATTGGGTGAATTTGCAAAAGTTTTGGAATTA
  GACAGTGTGAAAAGTGAAATTGTTCCACTGTTCACTAGTCTAGCTTCAGATGAACAGGATTCAGTGCGCC
  TCCTTGCTGTGGAAGCTTGTGTCAGTATTGCCCAGTTATTGTCTCAGGATGACCTTGAGACTTTGGTGAT
  GCCTACACTTCGACAAGCAGCAGAAGATAAATCTTGGCGCGTTCGCTATATGGTGGCTGACAGATTTTCA
  GAGCTCCAGAAAGCCATGGGTCCTAAAATCACCCTAAATGACCTCATCCCCGCCTTTCAGAACCTACTTA
  AAGACTGTGAAGCTGAAGTCCGGGCAGCTGCTGCCCACAAAGTAAAAGAACTTGGTGAGAACTTGCCCAT
  TGAAGATAGAGAGACCATAATTATGAATCAAATTCTGCCTTATATAAAGGAATTAGTATCCGATACCAAT
  CAACATGTCAAATCGGCTCTAGCTTCTGTAATTATGGGATTGTCTACTATTTTGGGCAAAGAAAATACCA
  TTGAACATCTTCTACCTCTTTTCTTAGCTCAGTTAAAGGATGAGTGTCCTGACGTTCGTTTGAATATCAT
  CTCCAATTTGGATTGTGTAAATGAAGTGATTGGAATCCGTCAGCTCTCTCAGTCTCTCCTTCCTGCCATA
  GTGGAGCTGGCAGAAGATGCCAAATGGAGGGTCCGCCTGGCCATCATTGAGTATATGCCGCTGCTGGCAG
  GCCAGCTGGGTGTGGAATTCTTTGATGAAAAGCTGAATTCTTTATGTATGGCTTGGCTCGTGGACCATGT
  ATACGCCATCCGAGAAGCTGCCACCAACAACCTCATGAAACTAGTTCAGAAGTTTGGTACAGAGTGGGCC
  CAAAATACTATTGTTCCCAAAGTGTTAGTAATGGCAAATGATCCTAATTACTTGCATAGAATGACCACTT
  TATTCTGCATTAATGCACTGTCTGAGGCCTGTGGTCAGGAAATAACTACTAAGCAAATGCTGCCCATCGT
  ATTAAAAATGGCAGGAGACCAAGTAGCAAATGTTCGCTTCAATGTGGCCAAATCTCTACAAAAGATTGGA
  CCAATTCTAGATACCAATGCTTTACAGGGAGAAGTGAAGCCAGTACTACAGAAGTTAGGTCAAGATGAAG
  ACATGGATGTCAAATACTTTGCACAGGAAGCTATAAGTGTTCTTGCATTGGCATAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002716 unedited
  CGGCCGCGAATTCGGCACCAGGCGCATCAGAGCTCGGGACCGGCCCAGGAGCAGCGGGTG
  GAGATGGAGATGATTCGCTATACCCGATCGCGGTTTTAATCGACGAGCTCCGCAATGAAG
  ACGTGCAGCTCCGACTCAACAGTATTAAGAAGTTATCAACAATTGCCCTAGCACTTGGAG
  TAGAAAGGACCCGAAGTGAATTGTTGCCATTTCTTACAGATACAATTTATGATGAAGATG
  AGGTACTATTAGCTCTTGCTGAGCAGCTGGGAAATTTCACTGGCCTAGTGGGAGGTCCTG
  ACTTTGCCCACTGTCTGCTGCCTCCTTTGGAAAATCTGGCAACTGTGGAAGAGACTGTTG
  TTCGTGACAAGGCTGTGGAGTCCCTGAGACAGATCTCCCAGGAGCATACTCCTGTTGCTC
  TGGAAGCTTATTTTGTACCTCTGGTGAAACGCTTAGCAAGTGGGGGATTGGTTCACCTCT
  CGCACATCTGCATGTGGTTTGTTCAGCGTTTGCTATCCCAGGGCATCAAATGCTGTTAAA
  GCAGAAATCAGACAGCAATTCCGTTCCTTGTGCTCAGATGACACCCAATGGTACGACGTG
  CTGCTGCTTCCAATTGGGTGAATTGGCAAAGTTTGGATTAGACAGGTGAAAGTGAATTGT
  TCACTGTCACTATCTACTTAGATGACAGATTAG
  
  3' Read Nucleotide Sequence

  >OriGene 3' genomic read for NM_002716 unedited
  GGACGCGGCCGCATTCTATGTCGAGTTTTTTTTTTTTTTTTTTTTATCCATTGAACACAT
  TTTTATTGAGCACCTATTATGTGCACCAGACACTGTTCCAGGCACTGGCGATACAGTAGA
  AGATAAAACAGACAAAAATACCAGCCTTACAGAGCTCCTAAAGCCTATATTCCAGTGAAA
  TCAAGATAGGAGAACTTTTCCCTTAAGTTTAAGGTAGTTAAAAAAAAAATAGGAGAACTA
  ATTCATGGAGAACATGGCCAAGTTATGAGCCAAAGCACAATTCCTCTGAAGAGTACCAAA
  ACAAATTCTCTCTCTATTGCTTAAGTCAGAAGGAAACAATGAAACCCCTGAGGGGTCACC
  ACAAAAGACAGAGGCCCCGCTGAACCCCCGACCCATGCTTGAGAAAGCCAGGGCCCACTC
  TCCCTCTCTCAAACAGTTTTAGGGTAGAGAAGTCAATGCTTAGGGCTCCTCACTGGGAGA
  CACAAGGGCATTTTAAAAGTCTGTCCCCAAAACAATGGCATTTCCAACCAGAGTAAAGCA
  ATGGTTCCAGTGCAAATCTGCTTCATGAAGACAAATGTCTCTCAAATATGAAATTCAATG
  AAGAAGACATAACTTGCGGTAACAAAATATNAGACTCATGAAACTAAAATTAAAATGTTC
  ACAGAATAATAGCCTAAGATTTAAAATGAGGATATAGAAAAAGATTTGGGCTTAGGGAAA
  TACCTAATTCCTGGCCCAAAGGCATAAAATAGAACTTACTATTAGGTATTTGGGGCGCTT
  AAAAGCAGACAGATACCTCTGATTAAAATTNCTATTTTTCGATTGTAGGCAAATTTTAAA
  GTTCTANGAAAACTTGGTTTTTGGACAAATTCAAAAGCTTAAAAGGGACTTGGGGCCTCA
  CCTAAAAAAAAGAACTGGTAGATTTAAAGAGGTGGGAACAAATTCACATTTAAAACCAAA
  GGGGACAGGAACCCTATAGGAAGGGAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002716
• Insert Size: 4860 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002716.3, NP_002707.3
• RefSeq Size: 5605 bp
• RefSeq ORF: 1806 bp
• Locus ID: 5519
• Cytogenetics: 11q23.1
• Domains: HEAT
• Protein Families: Druggable Genome, Phosphatase, Transcription Factors
• Protein Pathways: Long-term depression, Oocyte meiosis, TGF-beta signaling pathway, Tight junction, Wnt signaling pathway
• Gene Summary: 'This gene encodes a constant regulatory subunit of protein phosphatase 2. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The constant regulatory subunit A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. This gene encodes a beta isoform of the constant regulatory subunit A. Mutations in this gene have been associated with some lung and colon cancers. Alternatively spliced transcript variants have been described. [provided by RefSeq, Apr 2010]'
  Transcript Variant: This variant (1) represents the longest transcript and encodes isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.