CD45 (PTPRC) (NM_080921) Human Untagged Clone

CAT#: SC128007

PTPRC (untagged)-Human protein tyrosine phosphatase, receptor type, C (PTPRC), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PTPRC
• Synonyms: B220; CD45; CD45R; GP180; L-CA; LCA; LY5; T200
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC128007 sequence for NM_080921 edited (data generated by NextGen Sequencing)
  ATGTATTTGTGGCTTAAACTCTTGGCATTTGGCTTTGCCTTTCTGGACACAGAAGTATTT
  GTGACAGGGCAAAGCCCAACACCTTCCCCCACTGATGCCTACCTTAATGCCTCTGAAACA
  ACCACTCTGAGCCCTTCTGGAAGCGCTGTCATTTCAACCACAACAATAGCTACTACTCCA
  TCTAAGCCAACATGTGATGAAAAATATGCAAACATCACTGTGGATTACTTATATAACAAG
  GAAACTAAATTATTTACAGCAAAGCTAAATGTTAATGAGAATGTGGAATGTGGAAACAAT
  ACTTGCACAAACAATGAGGTGCATAACCTTACAGAATGTAAAAATGCGTCTGTTTCCATA
  TCTCATAATTCATGTACTGCTCCTGATAAGACATTAATATTAGATGTGCCACCAGGGGTT
  GAAAAGTTTCAGTTACATGATTGTACACAAGTTGAAAAAGCAGATACTACTATTTGTTTA
  AAATGGAAAAATATTGAAACCTTTACTTGTGATACACAGAATATTACCTACAGATTTCAG
  TGTGGTAATATGATATTTGATAATAAAGAAATTAAATTAGAAAACCTTGAACCCGAACAT
  GAGTATAAGTGTGACTCAGAAATACTCTATAATAACCACAAGTTTACTAACGCAAGTAAA
  ATTATTAAAACAGATTTTGGGAGTCCAGGAGAGCCTCAGATTATTTTTTGTAGAAGTGAA
  GCTGCACATCAAGGAGTAATTACCTGGAATCCCCCTCAAAGATCATTTCATAATTTTACC
  CTCTGTTATATAAAAGAGACAGAAAAAGATTGCCTCAATCTGGATAAAAACCTGATCAAA
  TATGATTTGCAAAATTTAAAACCTTATACGAAATATGTTTTATCATTACATGCCTACATC
  ATTGCAAAAGTGCAACGTAATGGAAGTGCTGCAATGTGTCATTTCACAACTAAAAGTGCT
  CCTCCAAGCCAGGTCTGGAACATGACTGTCTCCATGACATCAGATAATAGTATGCATGTC
  AAGTGTAGGCCTCCCAGGGACCGTAATGGCCCCCATGAACGTTACCATTTGGAAGTTGAA
  GCTGGAAATACTCTGGTTAGAAATGAGTCGCATAAGAATTGCGATTTCCGTGTAAAAGAT
  CTTCAATATTCAACAGACTACACTTTTAAGGCCTATTTTCACAATGGAGACTATCCTGGA
  GAACCCTTTATTTTACATCATTCAACATCTTATAATTCTAAGGCACTGATAGCATTTCTG
  GCATTTCTGATTATTGTGACATCAATAGCCCTGCTTGTTGTTCTCTACAAAATCTATGAT
  CTACATAAGAAAAGATCCTGCAATTTAGATGAACAGCAGGAGCTTGTTGAAAGGGATGAT
  GAAAAACAACTGATGAATGTGGAGCCAATCCATGCAGATATTTTGTTGGAAACTTATAAG
  AGGAAGATTGCTGATGAAGGAAGACTTTTTCTGGCTGAATTTCAGAGCATCCCGCGGGTG
  TTCAGCAAGTTTCCTATAAAGGAAGCTCGAAAGCCCTTTAACCAGAATAAAAACCGTTAT
  GTTGACATTCTTCCTTATGATTATAACCGTGTTGAACTCTCTGAGATAAACGGAGATGCA
  GGGTCAAACTACATAAATGCCAGCTATATTGATGGTTTCAAAGAACCCAGGAAATACATT
  GCTGCACAAGGTCCCAGGGATGAAACTGTTGATGATTTCTGGAGGATGATTTGGGAACAG
  AAAGCCACAGTTATTGTCATGGTCACTCGATGTGAAGAAGGAAACAGGAACAAGTGTGCA
  GAATACTGGCCGTCAATGGAAGAGGGTACTCGGGCTTTTGGAGATGTTGTTGTAAAGATC
  AACCAGCACAAAAGATGTCCAGATTACATCATTCAGAAATTGAACATTGTAAATAAAAAA
  GAAAAAGCAACTGGAAGAGAGGTGACTCACATTCAGTTCACCAGCTGGCCAGACCACGGG
  GTGCCTGAGGATCCTCACTTGCTCCTCAAACTGAGAAGGAGAGTGAATGCCTTCAGCAAT
  TTCTTCAGTGGTCCCATTGTGGTGCACTGCAGTGCTGGTGTTGGGCGCACAGGAACCTAT
  ATCGGAATTGATGCCATGCTAGAAGGCCTGGAAGCCGAGAACAAAGTGGATGTTTATGGT
  TATGTTGTCAAGCTAAGGCGACAGAGATGCCTGATGGTTCAAGTAGAGGCCCAGTACATC
  TTGATCCATCAGGCTTTGGTGGAATACAATCAGTTTGGAGAAACAGAAGTGAATTTGTCT
  GAATTACATCCATATCTACATAACATGAAGAAAAGGGATCCACCCAGTGAGCCGTCTCCA
  CTAGAGGCTGAATTCCAGAGACTTCCTTCATATAGGAGCTGGAGGACACAGCACATTGGA
  AATCAAGAAGAAAATAAAAGTAAAAACAGGAATTCTAATGTCATCCCATATGACTATAAC
  AGAGTGCCACTTAAACATGAGCTGGAAATGAGTAAAGAGAGTGAGCATGATTCAGATGAA
  TCCTCTGATGATGACAGTGATTCAGAGGAACCAAGCAAATACATCAATGCATCTTTTATA
  ATGAGCTACTGGAAACCTGAAGTGATGATTGCTGCTCAGGGACCACTGAAGGAGACCATT
  GGTGACTTTTGGCAGATGATCTTCCAAAGAAAAGTCAAAGTTATTGTTATGCTGACAGAA
  CTGAAACATGGAGACCAGGAAATCTGTGCTCAGTACTGGGGAGAAGGAAAGCAAACATAT
  GGAGATATTGAAGTTGACCTGAAAGACACAGACAAATCTTCAACTTATACCCTTCGTGTC
  TTTGAACTGAGACATTCCAAGAGGAAAGACTCTCGAACTGTGTACCAGTACCAATATACA
  AACTGGAGTGTGGAGCAGCTTCCTGCAGAACCCAAGGAATTAATCTCTATGATTCAGGTC
  GTCAAACAAAAACTTCCCCAGAAGAATTCCTCTGAAGGGAACAAGCATCACAAGAGTACA
  CCTCTACTCATTCACTGCAGGGATGGATCTCAGCAAACGGGAATATTTTGTGCTTTGTTA
  AATCTCTTAGAAAGTGCGGAAACAGAAGAGGTAGTGGATATTTTTCAAGTGGTAAAAGCT
  CTACGCAAAGCTAGGCCAGGCATGGTTTCCACATTCGAGCAATATCAATTCCTATATGAC
  GTCATTGCCAGCACCTACCCTGCTCAGAATGGACAAGTAAAGAAAAACAACCATCAAGAA
  GATAAAATTGAATTTGATAATGAAGTGGACAAAGTAAAGCAGGATGCTAATTGTGTTAAT
  CCACTTGGTGCCCCAGAAAAGCTCCCTGAAGCAAAGGAACAGGCTGAAGGTTCTGAACCC
  ACGAGTGGCACTGAGGGGCCAGAACATTCTGTCAATGGTCCTGCAAGTCCAGCTTTAAAT
  CAAGGTTCATAG
  
  Clone variation with respect to NM_080921.2
  1827 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_080921 unedited
  NTTTGCGGNATTTTGTAATACGACTCACTTATAGGGCGGCCGCGAATTCGCACGAGGTCT
  GATAAGACAACAGTGGAGAAAGGACGCATGCTGTTTCTTAGGGACACGGCTGACTTCCAG
  ATATGACCATGTATTTGTGGCTTAAACTCTTGGCATTTGGCTTTGCCTTTCTGGACACAG
  AAGTATTTGTGACAGGGCAAAGCCCAACACCTTCCCCCACTGATGCCTACCTTAATGCCT
  CTGAAACAACCACTCTGAGCCCTTCTGGAAGCGCTGTCATTTCAACCACAACAATAGCTA
  CTACTCCATCTAAGCCAACATGTGATGAAAAATATGCAAACATCACTGTGGATTACTTAT
  ATAACAAGGAAACTAAATTATTTACAGCAAAGCTAAATGTTAATGAGAATGTGGAATGTG
  GAAACAATACTTGCACAAACAATGAGGTGCATAACCTTACAGAATGTAAAAATGCGTCTG
  TTTCCATATCTCATAATTCATGTACTGCTCCTGATAAGACATTAATATTAGATGTGCCAC
  CAGGGGTTGAAAAGTTTCAGTTACATGATTGTACACAAGTTGAAAAAGCAGATACTACTA
  TTTGTTTAAAATGGAAAAATATTGAAACCTTTACTTGTGATACACAGAATATTACCTACA
  GATTTCAGTGTGGTAATATGATATTTGATAATAAAGAAATTAAATTAGAAAACCTTGAAC
  CCGAACATGAGTATAAGTGTGACTCAGAAATACTCTATAATAACCACAAGTTTACTTAAC
  GCAAGTAAATTATTAAAACAGATTTTGGGAGTCCAGGAGAGCCTCAGATATTTTTTGTAG
  AAAGTGAGCTGCACATCAAGGAGTAATTACCTGGAATCCCCTCAAAGACATTTCATAATT
  TACCCTCTGTATATAAAGAGACAGAAAAGATGCCTCATCTGA
  
  3' Read Nucleotide Sequence

  >Forward primer walk for NM_080921 unedited
  AAAAAAGCTCCTAAGACGTGNGCTTTTNCTTTGTTTTTATGNAGNAAAAAAACATTTTAT
  ATTAGAAGTGTTAACTTAGCTTGAAGGATCTGTTTTTAAAATCATAAACTGTGTGCAGAC
  TCAATGAAATCATGTGCACTTTCTGAAATGACACTGAAGGAGTCCTCCATCGGCCGCCCC
  GAAAGTAGCCCGCGGGCGCGCGGGGGGGGCCCGAGGCCCGGGCTGGGCCCCGTGCGGGGC
  CGCCGGGCCCCCGGCCGCGGCCGCCGCTGGTCCCCCCGGGCTCGGCCGCCAGCACGCCGG
  CCCCCCCACCGCCCGGACCGAGCTCCGGGGGGCCCCCGCCCCAGCGGCCGCAGGGGCGCG
  TGGCTCACCGGCGGAACGCGGGCCCCCGCCCGGCGCCTCGCGGGGCGGTCCGCCGGCCCG
  CCCGGGTTGCTGGGGCCGCCCCGCGGGCGGCCAACCTCGCCCGCCGCCGCGCGGTGCGCG
  CCCCCGCTGTTCCGGCGCGAGCCGGTGGGCGGGGGCGCGGCCGCCCAGTAGCCGCCCGCC
  CCGGACCCGGGGCGCGCCCCCACCGCCCTGCGAGACCCCGGGCCGTGTATGGGGCGCTCT
  TTCAGCCTGCGCCCGGCTTGTGCGCCCCGCCCGCGCGCGACGGGGACGAGGTGGTCCGGG
  CTGCTGGCCGCGATCGCCGGCCGCGGATCGCGGCGCGCTGATCGCCCCCCCGCGCGCCCC
  CGTGTTCTCCCCCCCCCCGCCACGCCCGTGCCCGCGCGCGCGCGCCCCCGAGATTGCTCC
  GGCCCGGTGACCCCCGCGGCGCGCGCGGCGCGTATGACACCCACGGCGCCCCCCGGCCGC
  GGGGTGTGCGACGCGGCGGGTGTGGCGCCCCCGCGCCTGTAGGCCGCCCCCGCCCGCGAG
  CCCGCCGAACGCCCCCGCCCGCCGCGGCTCCCGCGCGGGGTGCCCCCGTCACGCCCGCGC
  CGCGCGCCGCGCGCAAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_080921
• Insert Size: 4700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_080921.1, NP_563578.1
• RefSeq Size: 4543 bp
• RefSeq ORF: 3432 bp
• Locus ID: 5788
• Cytogenetics: 1q31.3-q32.1
• Domains: Y_phosphatase, FN3
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Phosphatase, Transmembrane
• Protein Pathways: Cell adhesion molecules (CAMs), Fc gamma R-mediated phagocytosis, Primary immunodeficiency, T cell receptor signaling pathway
• Gene Summary: 'The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported. [provided by RefSeq, Jun 2012]'
  Transcript Variant: This variant (2) lacks three in-frame exons in the 5' coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1.