PDP1 (NM_018444) Human Untagged Clone

CAT#: SC128049

PDP1 (untagged)-Human pyruvate dehyrogenase phosphatase catalytic subunit 1 (PDP1), nuclear gene encoding mitochondrial protein, transcript variant 5

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PDP1
• Synonyms: PDH; PDP; PDPC; PPM2A; PPM2C
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC128049 sequence for NM_018444 edited (data generated by NextGen Sequencing)
  ATGCCAGCACCAACTCAACTGTTTTTTCCTCTCATCCGTAACTGTGAACTGAGCAGGATC
  TATGGCACTGCATGTTACTGCCACCACAAACATCTCTGTTGTTCCTCATCGTACATTCCT
  CAGAGTCGACTGAGATACACACCTCATCCAGCATATGCTACCTTTTGCAGGCCAAAGGAG
  AACTGGTGGCAGTACACCCAAGGAAGGAGATATGCTTCCACACCACAGAAATTTTACCTC
  ACACCTCCACAAGTCAATAGCATCCTTAAAGCTAATGAATACAGTTTCAAAGTGCCAGAA
  TTTGACGGCAAAAATGTCAGTTCTATCCTTGGATTTGACAGCAATCAGCTGCCTGCAAAT
  GCACCCATTGAGGACCGGAGAAGTGCAGCAACCTGCTTGCAGACCAGAGGGATGCTTTTG
  GGGGTTTTTGATGGCCATGCAGGTTGTGCTTGTTCCCAGGCAGTCAGTGAAAGACTCTTT
  TATTATATTGCTGTCTCTTTGTTACCCCATGAGACTTTGCTAGAGATTGAAAATGCAGTG
  GAGAGCGGCCGGGCACTGCTACCCATTCTCCAGTGGCACAAGCACCCCAATGATTACTTT
  AGTAAGGAGGCATCCAAATTGTACTTTAACAGCTTGAGGACTTACTGGCAAGAGCTTATA
  GACCTCAACACTGGTGAGTCGACTGATATTGATGTTAAGGAGGCTCTAATTAATGCCTTC
  AAGAGGCTTGATAATGACATCTCCTTGGAGGCGCAAGTTGGTGATCCTAATTCTTTTCTC
  AACTACCTGGTGCTTCGAGTGGCATTTTCTGGAGCCACTGCTTGTGTGGCCCATGTGGAT
  GGTGTTGACCTTCATGTGGCCAATACTGGCGATAGCAGAGCCATGCTGGGTGTGCAGGAA
  GAGGACGGCTCATGGTCAGCAGTCACGCTGTCTAATGACCACAATGCTCAAAATGAAAGA
  GAACTAGAACGGCTGAAATTGGAACATCCAAAGAGTGAGGCCAAGAGTGTCGTGAAACAG
  GATCGGCTGCTTGGCTTGCTGATGCCATTTAGGGCATTTGGAGATGTAAAGTTCAAATGG
  AGCATTGACCTTCAAAAGAGAGTGATAGAATCTGGCCCAGACCAGTTGAATGACAATGAA
  TATACCAAGTTTATTCCTCCTAATTATCACACACCTCCTTATCTCACTGCTGAGCCAGAG
  GTAACTTACCACCGATTAAGGCCACAGGATAAGTTTCTGGTGTTGGCTACTGATGGGTTG
  TGGGAGACTATGCATAGGCAGGATGTGGTTAGGATTGTGGGTGAGTACCTAACTGGCATG
  CATCACCAACAGCCAATAGCTGTTGGTGGCTACAAGGTGACTCTGGGACAGATGCATGGC
  CTTTTAACAGAAAGGAGAACCAAAATGTCCTCGGTATTTGAGGATCAGAACGCAGCAACC
  CATCTCATTCGCCACGCTGTGGGCAACAACGAGTTTGGGACTGTTGATCATGAGCGCCTC
  TCTAAAATGCTTAGTCTTCCTGAAGAGCTTGCTCGAATGTACAGAGATGACATTACAATC
  ATTGTAGTTCAGTTCAATTCTCATGTTGTAGGGGCGTATCAAAACCAAGAATAG
  
  Clone variation with respect to NM_018444.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_018444 unedited
  NTTGTTCGGATTTGTATACGACTCACTATAGGGCGGCCGCGAATTCGCACGAGGGCGGGA
  AGCACTCCGGCCCGAACGTGCGGGCGGGGCAGGGTGGCGGCCCCGCACGGTAGGGGAGCA
  GAGTGGGCAGGCCGGGGGTGAGGGCTCGCGCTCCGGGAGCTGCACGGGGCTGCGTGGAAA
  GAGCGCCGAGCGGTGGCGTCGTTGTCGCCCCCTCCTCGTCGGGAAGAATCGTTTGGTCTC
  CTGCCGTGCCCGGAATCCCAGTCAGAAGTTCCAGCCTGCCACTGTTCTCTGATGCCATGC
  CAGCACCAACTCAACTGTTTTTTCCTCTCATCCGTAACTGTGAACTGAGCAGGATCTATG
  GCACTGCATGTTACTGCCACCACAAACATCTCTGTTGTTCCTCATCGTACATTCCTCAGA
  GTCGACTGAGATACACACCTCATCCAGCATATGCTACCTTTTGCAGGCCAAAGGAGAACT
  GGTGGCAGTACACCCAAGGAAGGAGATATGCTTCCACACCACAGAAATTTTACCTCACAC
  CTCCACAAGTCAATAGCATCCTTAAAGCTAATGAATACAGTTTCAAAGTGCCAGAATTTG
  ACGGCAAAAATGTCAGTTCTATCCTTGGATTTGACAGCAATCAGCTGCCTGCAAATGCAC
  CCATTGAGGACCGGAGAAGTGCAGCAACCTGCTTGCAGACCAGAGGGATGCTTTTGGGGG
  TTTTTGATGGCCATGCANGTTGTGCTTGTTCCCAGCAGTCAGTGAAAGACTCTTTTATTA
  TATTGCTGTCTCTTTGTTACCCCATGAGACTTTGCTAGAGATTGAAAATGCAGTGGAGAG
  CGGCCGGGCACTGCTACCCATTCTNCAGTGGCACAAGCACCCCATGATTACTTTAGTAAG
  GNAGCATTCAAATGTACTTTACAGCTTGAGGACTACTGGCAAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' genomic read for NM_018444 unedited
  TTTGGCCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTGAAGTTTACATGT
  ATTCTTTAATTCTAGACACCGTACAACAGTGACAACCAATTACAATAAAATCACAATTGC
  TTTTAGATGACAGTACTTTCAGATTTCTAATACCCAATTACTTTCATTTCCACAATGTCA
  ACTTCATGCTGCATTTTCATTTCTATAGAGCAGACAAGCTTCCAGACTGCAGACCAAGTT
  TCTTGTGTAATAATACTACTATCTTGATCATGACCACAGGAAACCAATTTTATATTCCCT
  GTACTATAGAGATGAGACATTATTTGGTGTATATGAAACTCTTCAGTGGTGGTGTTCAAG
  AATATTCAAATAGTAGCTGAAAATAGGGTTTGCTAGAGCAGTCCACATATTTCATTAAAA
  GAAAAATGCCCAGTCAAAACATTTAGAAATAAATATATAGTACAGCCTTTCCCTCTCCCA
  AATACTACCCAGGAAAAAAGTTCCAAGCTGACTTAAAAAAAAAAAAAACACACAAATAAA
  AACAAAAACACCTCAAAAAATAAACATTATATGATATTCTGAGCAGCAATCTTCCTGAGT
  CTATTTTCAATTGAAACAAATTCTGCAGCCAATAGTACACTGATTCAGTATCCACAGTCC
  AGAAGGAGTTCTTGAACATCTAGTTCATATCCCCAAAAAATGTGAAAAGACTCCTAAACA
  CCCAAGTTATTACACAATGGNGAAGCTGATATTTGGCTTGTATTAAAATCCAGGTGAATA
  TCANACATAATCACAGCCCTTACAGTGATGCTAGAATAGTGATGACAATATGTGCACAGC
  TGTNTACAGATCACTATTAACACCAGGACAAATGCCCCTTGATGCTATCAAAAGCCCAAT
  TGATATATTATTTCATTACATGATCAGNATAGAAGGACTGATATATATGGGGACGCTGCA
  ACATTGATGCCCTAGTTTCTATAAGCTCG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_018444
• ORF Size: 1614 bp
• Insert Size: 4260
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_018444.2, NP_060914.2
• RefSeq Size: 4215
• RefSeq ORF: 1614
• Locus ID: 54704
• Domains: PP2C
• Protein Families: Druggable Genome, Phosphatase
• Gene Summary: Pyruvate dehydrogenase (E1) is one of the three components (E1, E2, and E3) of the large pyruvate dehydrogenase complex. Pyruvate dehydrogenase kinases catalyze phosphorylation of serine residues of E1 to inactivate the E1 component and inhibit the complex. Pyruvate dehydrogenase phosphatases catalyze the dephosphorylation and activation of the E1 component to reverse the effects of pyruvate dehydrogenase kinases. Pyruvate dehydrogenase phosphatase is a heterodimer consisting of catalytic and regulatory subunits. Two catalytic subunits have been reported; one is predominantly expressed in skeletal muscle and another one is is much more abundant in the liver. The catalytic subunit, encoded by this gene, is the former, and belongs to the protein phosphatase 2C (PP2C) superfamily. Along with the pyruvate dehydrogenase complex and pyruvate dehydrogenase kinases, this enzyme is located in the mitochondrial matrix. Mutation in this gene causes pyruvate dehydrogenase phosphatase deficiency. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jun 2009]
  Transcript Variant: This variant (5) lacks an internal segment, compared to variant 2. The difference causes translation initiation at a downstream AUG and results in an isoform (3) with a shorter N-terminus, compared to isoform 2.