FGD4 (NM_139241) Human Untagged Clone

CAT#: SC128121

FGD4 (untagged)-Human FYVE, RhoGEF and PH domain containing 4 (FGD4)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: FGD4
• Synonyms: CMT4H; FRABP; ZFYVE6
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC128121 sequence for NM_139241 edited (data generated by NextGen Sequencing)
  ATGGAGGAAATTAAACCTGCCTCTGCTTCTTGTGTCTCAAAAGAAAAACCCAGTAAGGTA
  TCAGATCTCATCAGTCGCTTTGAAGGAGGCAGCTCATTATCAAATTATAGTGATTTGAAG
  AAAGAGTCTGCTGTGAACCTAAATGCTCCTAGAACCCCAGGAAGGCATGGATTGACAACC
  ACACCTCAACAAAAACTCCTCTCCCAGCACTTGCCACAGAGGCAGGGAAATGATACAGAT
  AAGACTCAGGGTGCACAGACTTGTGTGGCCAACGGTGTAATGGCAGCACAAAACCAGATG
  GAATGTGAGGAGGAGAAAGCTGCCACTCTTAGCTCAGATACTTCTATTCAAGCTTCTGAA
  CCCTTGCTTGATATGCACATAGTGAATGGAGAAAGAGATGAAACTGCCACAGCTCCTGCA
  TCACCCACAACAGACAGCTGTGATGGAAATGCTTCTGACAGTAGCTACAGGACTCCAGGC
  ATAGGCCCAGTGCTCCCCCTAGAAGAAAGAGGGGCAGAAACAGAAACCAAGGTACAAGAG
  AGGGAAAATGGGGAAAGCCCTCTGGAACTGGAGCAGCTGGACCAGCACCATGAGATGAAG
  GAGACTAATGAGCAAAAACTTCACAAAATAGCCAATGAACTTTTGCTTACTGAAAGAGCT
  TATGTCAACCGACTTGACCTCTTAGATCAGGTATTTTATTGCAAACTGTTGGAAGAAGCA
  AACCGAGGCTCGTTTCCAGCAGAGATGGTGAATAAAATCTTTTCTAATATTTCATCAATA
  AATGCCTTCCATAGTAAATTCCTCTTGCCAGAGCTGGAGAAACGAATGCAAGAATGGGAA
  ACTACTCCTAGAATTGGAGACATCCTTCAGAAATTGGCACCATTCCTTAAGATGTATGGA
  GAATATGTGAAAGGATTTGATAATGCAATGGAATTGGTTAAAAACATGACAGAACGTATT
  CCCCAGTTCAAATCAGTGGTTGAAGAAATTCAGAAACAGAAAATCTGTGGGAGCTTAACT
  TTGCAGCATCACATGCTAGAACCTGTTCAGCGGATTCCCCGGTATGAGATGCTCCTTAAG
  GACTATCTAAGGAAATTGCCTCCTGATTCCCTGGACTGGAATGATGCTAAAAAATCACTT
  GAAATTATATCTACAGCAGCAAGCCATTCTAATAGTGCAATAAGGAAAATGGAGAACCTA
  AAGAAACTCTTAGAGATTTATGAAATGTTGGGAGAAGAAGAAGACATTGTAAACCCTTCA
  AATGAACTAATAAAAGAAGGACAGATCCTCAAACTAGCTGCTCGAAACACTTCAGCACAA
  GAACGCTACCTTTTCTTATTCAACAACATGTTGCTGTACTGTGTGCCCAAATTCAGCTTG
  GTAGGCTCTAAATTCACAGTTCGAACCAGGGTTGGCATTGATGGAATGAAAATTGTAGAG
  ACTCAAAATGAAGAATATCCACATACTTTCCAGGTGTCTGGGAAAGAGAGAACACTGGAA
  CTGCAGGCCAGTTCTGCACAAGACAAAGAAGAATGGATCAAGGCCCTTCAAGAAACCATC
  GATGCTTTTCATCAAAGGCATGAAACCTTCAGAAATGCAATTGCAAAGGATAATGACATT
  CACTCAGAGGTTTCTACTGCTGAGCTAGGGAAAAGAGCCCCAAGATGGATCCGAGATAAT
  GAAGTGACAATGTGTATGAAATGTAAAGAACCTTTCAATGCACTGACACGAAGGAGGCAT
  CATTGTCGAGCATGTGGATATGTGGTTTGTTGGAAATGCTCCGACTACAAAGCTCAACTT
  GAATATGATGGTGGTAAATTGAGCAAAGTTTGTAAAGACTGTTATCAAATCATAAGTGGA
  TTCACAGACAGTGAAGAAAAGAAAAGAAAAGGAATTTTAGAGATTGAATCAGCAGAAGTA
  TCTGGAAACAGTGTGGTGTGCAGCTTTCTTCAGTATATGGAGAAGTCAAAACCTTGGCAG
  AAAGCTTGGTGTGTGATCCCCAAGCAAGACCCTCTTGTGCTGTACATGTATGGTGCCCCC
  CAGGACGTCAGAGCCCAGGCCACCATTCCACTTCTGGGCTATGTGGTGGATGAAATGCCA
  AGGAGCGCAGACCTGCCACACAGTTTCAAACTGACCCAGTCTAAGTCCGTGCACAGCTTT
  GCTGCAGACAGTGAGGAACTGAAGCAGAAGTGGCTGAAAGTCATCCTTTTAGCTGTCACA
  GGTGAGACACCAGGTGGTCCAAATGAGCATCCAGCCACCTTGGATGATCATCCTGAACCT
  AAGAAAAAATCAGAATGCTGA
  
  Clone variation with respect to NM_139241.2
  374 c=>t;1305 g=>a;1518 g=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_139241 unedited
  GGGGTTCNCATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGCAGGGGA
  GCCTGTGCTGGGTGAGATTATCTGCAATGGACCGGGTTAATGGGAGGCTTGCTTTGGCTC
  GCTGGAAGAACAAATGAAGGGAGTTTTACTTCAACCATTTGTGACTTCTGGCCAAGAGGA
  ATAAATTCAGCTTTTATAAGGCAGCAGTACCTGTCCAAAGATCGCTTTAGTTCCACCTTG
  CTCCACAAGCAGCACAACCACACTGGTTGGTGAGAATGTATCTGAAGAAGAGGCTCAGGG
  AATAAATGGGAACAGGCCAGCAAAACACTCAGCTGCAAGTCCAAAGCCACAAGTGCCTCC
  AAAGCCATTACACCTGCAGAATTCACCTTCGTCCAATATACACCAAACCCCCAGGCATAA
  AGCTTTACCTAGTGCAAAACCAAGGATGGAGGAAATTAAACCTGCCTCTGCTTCTTGTGT
  CTCAAAAGAAAAACCCAGTAAGGTATCAGATCTCATCAGTCGCTTTGAAGGAGGCAGCTC
  ATTATCAAATTATAGTGATTTGAAGAAAGAGTCTGCTGTGAACCTAAATGCTCCTAGAAC
  CCCAGGAAGGCATGGATTGACAACCACACCTCAACAAAAACTCCTCTCCCAGCACTTGCC
  ACAGAGGCAGGGAAATGATACAGATAAGACTCAGGGTGCACAGACTTGTGTGGCCAACGG
  TGTAATGGCAGCACAAAACCAGATGGAATGTGAGGAGGAGAAAGCTGCCACTCTTAGCTC
  AGAACTTCTATTCAAGCTTCTGAACCCTTGCTTGATATGCACATAGTGAATGGAGAAAGA
  GATGAAACTGCCACAGCTCCTGCATCACCCACACAGACAGCTGTGATGGAAATGCTTCTG
  ACAGTAGCTACAGGACTTCCAGCTAC
  
  3' Read Nucleotide Sequence

  >OriGene 3' genomic read for NM_139241 unedited
  GGGGCGGTTGTACATCTCAAAAAAGGTTTAATTTGNATAAAAAGCATAAGTTAAATAGTA
  AACTTAGTGTGAGATTTTAATCACAAGTACAAAAGAGTGAAGAGCAGCCTTCATGACAAG
  GAATCATGTGACCAGCCCCCACCCCAAACATAGGTTATCACATGTGGATTTTGTTTCATT
  CCGACAAAGGAAAAGGCCCATACAGTTATCTTAAGTGTTCATTATATAGAGACCTTATTT
  AACCATGCTCGATTATCTTACGGAAAAGAAAAACATGACGATAAATGTGGTTTCCACATA
  TCCTAGTTCTGTAAAAAGTCAAGTGTCTCATAAAGTTTGGGTTTTGGTGAAAATGTTTGT
  ACATGCTGATGTTAACTACTGCAATAATTATAGGATTGTTAACGGGTAAATAACCTGTAT
  GACTTTGACTGTCCCACTGACCAATATGCACTTTAATCTTACAATAATCAAGACTGACGC
  ATTAACTGAACCCTCATCTTATATTAATAGAACCTCTCTAGAGAGGCACTGTTTTAAATA
  GGCCCTGACACAAAAAATGGGCCTTTAAAAGTACTGTTCGGAATTCACAAATTAGTGCCA
  TGCCACATAAATTACCAATTTTTGTAAAAATATCAATCCCCTCCAATGCCAAATCGCAAA
  ACCTAAATCACCCGAAACATTTCTTACATTTCAGAACATTGCTCAGTTCCCACGGTACAC
  CTGGCTTTCATATTTAAAAATCAATTAGGGATTAATGTGGACCCTTACACCATGTCTAAC
  ATATATATTTAACTCCACATAAACCAAATTGCTATTTCCTTAAGTACATTCTTAAGCAGT
  CTTATTTGCAACACCATTATATTCCTTTCCTTTTTCAGTCGCGAAATACTAC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_139241
• ORF Size: 2301 bp
• Insert Size: 4500
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_139241.1, NP_640334.1
• RefSeq Size: 2931
• RefSeq ORF: 2301
• Locus ID: 121512
• Domains: RhoGEF, FYVE, PH
• Gene Summary: This gene encodes a protein that is involved in the regulation of the actin cytoskeleton and cell shape. This protein contains an actin filament-binding domain, which together with its Dbl homology domain and one of its pleckstrin homology domains, can form microspikes. This protein can activate MAPK8 independently of the actin filament-binding domain, and it is also involved in the activation of CDC42 via the exchange of bound GDP for free GTP. The activation of CDC42 also enables this protein to play a role in mediating the cellular invasion of Cryptosporidium parvum, an intracellular parasite that infects the gastrointestinal tract. Mutations in this gene can cause Charcot-Marie-Tooth disease type 4H (CMT4H), a disorder of the peripheral nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2015]
  Transcript Variant: This variant (3) has an alternate 5' terminal exon, which results in a different 5' UTR and translation initiation at a downstream AUG start codon, compared to variant 1. The resulting isoform (3) has a shorter N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.