EGLN1 (NM_022051) Human Untagged Clone

CAT#: SC128146

EGLN1 (untagged)-Human egl nine homolog 1 (C. elegans) (EGLN1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: EGLN1
• Synonyms: C1orf12; ECYT3; HALAH; HIF-PH2; HIFPH2; HPH-2; HPH2; PHD2; SM20; ZMYND6
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_022051, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCCAATGACAGCGGCGGGCCCGGCGGGCCGAGCCCGAGCGAGCGAGACCGGCAGTACTGCGAGCTGT
  GCGGGAAGATGGAGAACCTGCTGCGCTGCAGCCGCTGCCGCAGCTCCTTCTACTGCTGCAAGGAGCACCA
  GCGTCAGGACTGGAAGAAGCACAAGCTCGTGTGCCAGGGCAGCGAGGGCGCCCTCGGCCACGGAGTGGGC
  CCACACCAGCATTCCGGCCCCGCGCCGCCGGCTGCAGTGCCGCCGCCCAGGGCCGGGGCCCGGGAGCCCA
  GGAAGGCAGCGGCGCGCCGGGACAACGCCTCCGGGGACGCGGCCAAGGGAAAAGTAAAGGCCAAGCCCCC
  GGCCGACCCAGCGGCGGCCGCGTCGCCGTGTCGTGCGGCCGCCGGCGGCCAGGGCTCGGCGGTGGCTGCC
  GAAGCCGAGCCCGGCAAGGAGGAGCCGCCGGCCCGCTCATCGCTGTTCCAGGAGAAGGCGAACCTGTACC
  CCCCAAGCAACACGCCCGGGGATGCGCTGAGCCCCGGCGGCGGCCTGCGGCCCAACGGGCAGACGAAGCC
  CCTGCCGGCGCTGAAGCTGGCGCTCGAGTACATCGTGCCGTGCATGAACAAGCACGGCATCTGTGTGGTG
  GACGACTTCCTCGGCAAGGAGACCGGACAGCAGATCGGCGACGAGGTGCGCGCCCTGCACGACACCGGGA
  AGTTCACGGACGGGCAGCTGGTCAGCCAGAAGAGTGACTCGTCCAAGGACATCCGAGGCGATAAGATCAC
  CTGGATCGAGGGCAAGGAGCCCGGCTGCGAAACCATTGGGCTGCTCATGAGCAGCATGGACGACCTGATA
  CGCCACTGTAACGGGAAGCTGGGCAGCTACAAAATCAATGGCCGGACGAAAGCCATGGTTGCTTGTTATC
  CGGGCAATGGAACGGGTTATGTACGTCATGTTGATAATCCAAATGGAGATGGAAGATGTGTGACATGTAT
  ATATTATCTTAATAAAGACTGGGATGCCAAGGTAAGTGGAGGTATACTTCGAATTTTTCCAGAAGGCAAA
  GCCCAGTTTGCTGACATTGAACCCAAATTTGATAGACTGCTGTTTTTCTGGTCTGACCGTCGCAACCCTC
  ATGAAGTACAACCAGCATATGCTACAAGGTACGCAATAACTGTTTGGTATTTTGATGCAGATGAGAGAGC
  ACGAGCTAAAGTAAAATATCTAACAGGTGAAAAAGGTGTGAGGGTTGAACTCAATAAACCTTCAGATTCG
  GTCGGTAAAGACGTCTTCTAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_022051 unedited
  TAATTACCCCCGCCCGTTGACGCAAAGGGCGGTAGGCGTGTCCGGTGGGAGGTCTATATA
  AGCAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCC
  GCGAATTCGGCACGAGGCCCGTATGCCCTGCGCTCCTCCACAGCCTGGGCCGGGCCGCCC
  GGGACGCTGAGGCGGCGGCGGCGGCCGAGGGGGCCGGTCTTGCGCTCCCCAGGCCCGCGC
  GCCTGAGCCCAGGTTGCCATTCGCCGCACAGGCCCTATTCTCTCAGCCCTCGGCGGCGAT
  GAGGCGCTGAGGCGGCTGCCGGCGCTGCGCCGGAGCTTAGGACTCGGAAGCGGCCGGGCC
  GAGGGCGTGGGGTGCCGGCCTCCCTGAGGCGAGGGTAGCGGGTGCATGGCGCAGTAACGG
  CCCCTATCTCTCTCCCCGCTCCCCAGCCTCGGGCGAGGCCGTCCGGCCGCTACCCCTCCT
  GCTCGGCCGCCGCAGTCGCCGTCGCCGCCGCCGCCGCCGCCATGGCCAATGACAGCGGCG
  GGCCCGGCGGGCCGAGCCCGAGCGAGCGAGACCGGCAGTACTGCGAGCTGTGCGGGAAGA
  TGGAGAACCTGCTGCGCTGCAGCCGCTGCCGCAGCTCCTTCTACTGCTGCCAGGAGCACC
  AGCGTCAGGACTGGAAGAAGCACAAGCTCGTGTGCCAGGGCAGCGAGGGCGCCCTCGGCC
  ACTGGAGTGGCCCCACACCANCATTACTGACCCGCGCCGTCGGCTGCAATGCCGCCGCCC
  AGGGCCGGGGCCCCGGAAGCCCAGCCAGGCCACCGGCGCTGCTGGGACACGCCTCCGGGG
  ACGCGGCCAGGGAAAAGTAAAGGCCAAGCCCCTGTCGACCCAGCGGGGGCGCGATCCACC
  TGTTCTTGCTGCCCACCGCCGCCAGAT
  
  3' Read Nucleotide Sequence

  >Forward primer walk for NM_022051 unedited
  NTTCCCATTTGTAANATGCTGTTTTTCTGGTCTGACCGTCGCAACCCTCATNAAGTACAA
  CCAGCATATGCTACAAGGTACGCAATAACTGTTTGGTATTTTGATGCAGATGAGAGAGCA
  CGAGCTAAAGTAAAATATCTAACAGGTGAAAAAGGTGTGAGGGTTGAACTCAATAAACCT
  TCAGATTCGGTCGGTAAAGACGTCTTCTAGAGCCTTTGATCCAGCAATACCCCACTTCAC
  CTACAATATTGTTAACTATTTGTTAACTTGTGAATACGAATAAATGGGATAAAGAAAAAT
  AGACAACCAGTTCGCATTTTAATAAGGAAACAGAAACAACTTTTTGTGTTGCATCAAACA
  GAAGATTTTGACTGCTGTGACTTTGTACTGCATGATCAACTTCAAATCTGTGATTGCTTA
  CAGGAGGAAGATAAGCTACTAATTGAAAATGGTTTTTACATCTGGATATGAAATAAGTGC
  CCTGTGTAGAATTTTTTTCATTCTTATATTTTGCCAGATCTGTTATCTAGCTGAGTTCAT
  TTCATCTCTCCCTTTTTTATATCAAGTTTGAATTTNGGATAATTTTTCTATATTAGGTAC
  AATTTATCTAAACTGAATTGAGAAAAAATTACAGTATTATTCCTCANATAACATCAATCT
  ATTTTTTGTAACCTGTTCATACTATTAAATTTTGCCCTAAAAGACCTCTTATAATTGATT
  GTTGCCAGTGACTGATGAATAAATTTTAATTTTACTTAANATAAAAAAAGGAGCACTTTA
  ATTACAACTGAAAATCAGAATGGTTTGTGAAGTCCTTCCCTTAACTAATTTGAACTGTTA
  AAGATGGTGGCCTTTTTTTTGGACAATGGCCATAACGAAACCTAATGGTAAAAAAGGCCC
  CATTTACTACCCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_022051
• ORF Size: 1281 bp
• Insert Size: 4050
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_022051.1, NP_071334.1
• RefSeq Size: 5163
• RefSeq ORF: 1281
• Locus ID: 54583
• Domains: zf-MYND, 2OG-FeII_Oxy, P4Hc
• Protein Pathways: Pathways in cancer, Renal cell carcinoma
• Gene Summary: The protein encoded by this gene catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. HIF is a transcriptional complex that plays a central role in mammalian oxygen homeostasis. This protein functions as a cellular oxygen sensor, and under normal oxygen concentration, modification by prolyl hydroxylation is a key regulatory event that targets HIF subunits for proteasomal destruction via the von Hippel-Lindau ubiquitylation complex. Mutations in this gene are associated with erythrocytosis familial type 3 (ECYT3). [provided by RefSeq, Nov 2009]