E2F5 (NM_001951) Human Untagged Clone

CAT#: SC128153

E2F5 (untagged)-Human E2F transcription factor 5, p130-binding (E2F5), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: E2F5
• Synonyms: E2F-5
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC128153 sequence for NM_001951 edited (data generated by NextGen Sequencing)
  ATGGCGGCGGCANNNCCCGCGAGCTCGGGCCAGCAGGCGCCGGCAGGGCAGGGGCAGGGC
  CAGCGGCCGCCGCCGCAGCCTCCGCAGGCGCAAGCCCCGCAGCCGCCCCCGCCGCCGCAG
  CTCGGGGGCGCGGGGGGCGGCAGCAGCAGGCACGAGAAGAGCCTGGGGCTGCTCACTACC
  AAGTTCGTGTCGCTGCTGCAGGAGGCCAAGGACGGCGTTCTGGATCTCAAAGCGGCTGCT
  GATACTTTGGCTGTGAGGCAAAAAAGGAGAATTTATGATATCACCAATGTCTTAGAGGGA
  ATTGACTTGATTGAAAAAAAGTCAAAAAACAGTATCCAGTGGAAAGGTGTAGGTGCTGGC
  TGTAATACTAAAGAAGTCATAGATAGATTAAGATATCTTAAAGCTGAAATTGAAGATCTA
  GAACTGAAGGAAAGAGAACTTGATCAGCAGAAGTTGTGGCTACAGCAAAGCATCAAAAAT
  GTGATGGACGATTCCATTAATAATAGATTTTCCTATGTAACTCATGAAGACATCTGTAAT
  TGCTTTAATGGTGATACACTTTTGGCCATTCAGGCACCTTCTGGTACACAACTGGAGGTA
  CCCATTCCAGAAATGGGTCAGAATGGACAAAAGAAATACCAGATCAATCTAAAGAGTCAT
  TCAGGACCTATCCATGTGCTGCTTATAAATAAAGAGTCGAGTTCATCTAAGCCCGTGGTT
  TTTCCTGTTCCCCCACCTGATGACCTCACACAGCCTTCCTCCCAGTCCTTGACTCCAGTG
  ACTCCACAGAAATCCAGCATGGCAACTCAAAATCTGCCTGAGCAACATGTCTCTGAAAGA
  AGCCAGGCTCTGCAGCAGACATCAGCTACAGATATATCTTCAGCAGGATCTATTAGTGGA
  GATATCATTGATGAGTTAATGTCTTCTGACGTGTTTCCTCTCTTAAGGCTTTCTCCTACC
  CCGGCAGATGACTACAACTTTAATTTAGATGATAACGAAGGAGTTTGTGATCTGTTTGAT
  GTCCAGATACTAAATTATTAG
  
  Clone variation with respect to NM_001951.3
  13 g=>n;14 a=>n;15 g=>n;132 c=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001951 unedited
  TTCGGAATTTGTAATACGACTCTACTATAGGGCGGCCGCGAATCGGCACCAGGAAGCGGC
  CGCAGTGGAGCCGACCCGGCAGGTGGCCGCGGGCGGGGCCGGCGAGCGAAAGTGCGCGGG
  GGCCCGACCACCGCGGGGCCGGGACGCGATGGCGGCGGCAGAGCCCGCGAGCTCGGGCCA
  GCAGGCGCCGGCAGGGCAGGGGCAGGGCCAGCGGCCGCCGCCGCAGCCTCCGCAGGCGCA
  AGCCCCGCAGCCGCCCCCGCCGCCGCAGCTCGGGGGCGCGGGGGGCGGCAGCAGCAGGCA
  CGAGAAGAGCCTGGGGCTGCTCACTACCAAGTTCGTGTCGCTGCTGCAGGAGGCCAAGGA
  CGGCGTTCTGGATCTCAAAGCGGCTGCTGATACTTTGGCTGTGAGGCAAAAAAGGAGAAT
  TTATGATATCACCAATGTCTTAGAGGGAATTGACTTGATTGAAAAAAAGTCAAAAAACAG
  TATCCAGTGGAAAGGTGTANGTGCTGGCTGTAATACTAAAGAAGTCATAGATAGATTAAG
  ATATCTTAAAGCTGAAATTGAAGATCTAGAACTGAAGGAAAGAGAACTTGATCAGCAGAA
  GTTGTGGCTACAGCAAAGCATCAAAAATGTGATGGACGATTCCATTAATAATAGATTTTC
  CTATGTAACTCATGAAGACATCTGTNATTGCTNTAATGGTGATACACTTTTGGCCATTCA
  GGCACCTTCTGGTACACAACTGGNAGTACCCCATCCANAAATGGGTCAGAATGACAANAG
  AATACCAGATCAATCTAAGAATCATTCAGACCTATCCTGTGCTGCTTATAATAAAGATCC
  ATTCATCTAGCCCGTGGTTTTTCTGTCCCCCACTGATGACTACCAGNCTNCTCCAGTCCT
  GACTCCCGGACTCCCCAAATCCGCTGGCACTCAAACTGC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001951 unedited
  NTAATTCAGNACGCGGCCGCATTCTANGATCAGTTTTTTTTTTTTTTTTTTGAATGTTTT
  ATACAATTTTATTTTTAAAAATCTTGTTAATGTACAGGCATTGGCACATTTTAAAAACAA
  ACTACATAAACAGATCTTTCCTATAACCTAGGAAAGTGGAATGTCAGAAGTCAACAAAAT
  GTGATAAACTTAAAGTGCTAAAACAGAAGGCACTTCACAAAATCTGTTCACTGAAACAGT
  TATATATCCTCGTTTACATCCTTCACTTTACAAGTGGCAGTGAACGTCTGTTTGGATAGA
  AGGACATACAGAAATACAGGCAGTTTAGTGGCAGTAAAAATATAAGACAAGTAATGAGTC
  CTTGGCCAACTTGTTTTTGATGACCTGTAGTGTCCTTTAACTTTCCTCCTTAAGAAGAAA
  AAACAGAAGGATTCACAATTAGAAGAAAAGTTGAAATCCAGAATATAGTTACTTTTGGGA
  GTGGGGACGTTTGTGGATATTAAATCACTTATCCCCTAAAGCAATCAGAGCCCTTTGTTT
  TTATGGTTGAAGTTTTGTGAAGTAAAGAAAGTATTAGGGAAGAACACTTCAGAATCAGTG
  AACTAAAAAAGGTGTTACATTCCTTATTTTAAATATTTAGGTTATTAAGAAGGCCAAAAT
  GTTACCCAGTTAGAGGTAGATAACAGTCCCAAGTTTTCCTGGAAATCTAAAATTTAATAT
  CCTGGACATCAAAAAAAATCACAAACTCCTTCGGTATCCTCCAAAATAAAGGTTGGAGTC
  CTCCGGCCCGGGTAAGGGGAAAGCCCTAAAAAAAGGAAACCCCCCGAAGAACATTACTCA
  CCAATGAAATCTCCACCAAAAAATCCGGGTGAAAAAATAACCTGGAACTGATGTCTGCTG
  CCGAACCTGGGTTTCTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001951
• ORF Size: 1041 bp
• Insert Size: 1700
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info

Reference Data

• RefSeq: NM_001951.2, NP_001942.1
• RefSeq Size: 1752
• RefSeq ORF: 1041
• Locus ID: 1875
• Domains: E2F_TDP
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Cell cycle, TGF-beta signaling pathway
• Gene Summary: The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionarily conserved domains that are present in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein is differentially phosphorylated and is expressed in a wide variety of human tissues. It has higher identity to E2F4 than to other family members. Both this protein and E2F4 interact with tumor suppressor proteins p130 and p107, but not with pRB. Alternative splicing results in multiple variants encoding different isoforms. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).