PML Protein (PML) (NM_033244) Human Untagged Clone

CAT#: SC128164

PML (untagged)-Human promyelocytic leukemia (PML), transcript variant 5

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PML
• Synonyms: MYL; PP8675; RNF71; TRIM19
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC128164 sequence for NM_033244 edited (data generated by NextGen Sequencing)
  ATGGAGCCTGCACCCGCCCGATCTCCGAGGCCCCAGCAGGACCCCGCCCGGCCCCAGGAG
  CCCACCATGCCTCCCCCCGAGACCCCCTCTGAAGGCCGCCAGCCCAGCCCCAGCCCCAGC
  CCTACAGAGCGAGCCCCCGCTTCGGAGGAGGAGTTCCAGTTTCTGCGCTGCCAGCAATGC
  CAGGCGGAAGCCAAGTGCCCGAAGCTGCTGCCTTGTCTGCACACGCTGTGCTCAGGATGC
  CTGGAGGCGTCGGGCATGCAGTGCCCCATCTGCCAGGCGCCCTGGCCCCTAGGTGCAGAC
  ACACCCGCCCTGGATAACGTCTTTTTCGAGAGTCTGCAGCGGCGCCTGTCGGTGTACCGG
  CAGATTGTGGATGCGCAGGCTGTGTGCACCCGCTGCAAAGAGTCGGCCGACTTCTGGTGC
  TTTGAGTGCGAGCAGCTCCTCTGCGCCAAGTGCTTCGAGGCACACCAGTGGTTCCTCAAG
  CACGAGGCCCGGCCCCTAGCAGAGCTGCGCAACCAGTCGGTGCGTGAGTTCCTGGACGGC
  ACCCGCAAGACCAACAACATCTTCTGCTCCAACCCCAACCACCGCACCCCTACGCTGACC
  AGCATCTACTGCCGAGGATGTTCCAAGCCGCTGTGCTGCTCGTGCGCGCTCCTTGACAGC
  AGCCACAGTGAGCTCAAGTGCGACATCAGCGCAGAGATCCAGCAGCGACAGGAGGAGCTG
  GACGCCATGACGCAGGCGCTGCAGGAGCAGGATAGTGCCTTTGGCGCGGTTCACGCGCAG
  ATGCACGCGGCCGTCGGCCAGCTGGGCCGCGCGCGTGCCGAGACCGAGGAGCTGATCCGC
  GAGCGCGTGCGCCAGGTGGTAGCTCACGTGCGGGCTCAGGAGCGCGAGCTGCTGGAGGCT
  GTGGACGCGCGGTACCAGCGCGACTACGAGGAGATGGCCAGTCGGCTGGGCCGCCTGGAT
  GCTGTGCTGCAGCGCATCCGCACGGGCAGCGCGCTGGTGCAGAGGATGAAGTGCTACGCC
  TCGGACCAGGAGGTGCTGGACATGCACGGTTTCCTGCGCCAGGCGCTCTGCCGCCTGCGC
  CAGGAGGAGCCCCAGAGCCTGCAAGCTGCCGTGCGCACCGATGGCTTCGACGAGTTCAAG
  GTGCGCCTGCAGGACCTCAGCTCTTGCATCACCCAGGGGAAAGATGCAGCTGTATCCAAG
  AAAGCCAGCCCAGAGGCTGCCAGCACTCCCAGGGACCCTATTGACGTTGACCTGCCCGAG
  GAGGCAGAGAGAGTGAAGGCCCAGGTTCAGGCCCTGGGGCTGGCTGAAGCCCAGCCTATG
  GCTGTGGTACAGTCAGTGCCCGGGGCACACCCCGTGCCAGTGTACGCCTTCTCCATCAAA
  GGCCCTTCCTATGGAGAGGATGTCTCCAATACAACGACAGCCCAGAAGAGGAAGTGCAGC
  CAGACCCAGTGCCCCAGGAAGGTCATCAAGATGGAGTCTGAGGAGGGGAAGGAGGCAAGG
  TTGGCTCGGAGCTCCCCGGAGCAGCCCAGGCCCAGCACCTCCAAGGCAGTCTCACCACCC
  CACCTGGATGGACCGCCTAGCCCCAGGAGCCCCGTCATAGGAAGTGAGGTCTTCCTGCCC
  AACAGCAACCACGTGGCCAGTGGCGCCGGGGAGGCAGGTAGGGAGAGGAACGCGTTGTGG
  TGA
  
  Clone variation with respect to NM_033244.3
  
  5' Read Nucleotide Sequence

  >Reverse primer walk for NM_033244 unedited
  CATAACAGGGACCAGCACAACGCGTACGTAAATCCTCGGCAGTACAGCTGGTCAGCGTAG
  GGGTGCGGTGGTTGGGGTTGGAGCAGAAGAGTTGTTGGTCTTGCGGGTGCCGTCCAGGAA
  CTCACGCACCGACTGGTTGCGCAGCTCTGCTAGGGGCCGGGCCTCGTGCTTGAGGAACCA
  CTGGTGTGCCTCGAAGCACTTGGCGCAGAGGAGCTGCTCGCACTCAAAGCACCAGAAGTC
  GGCCGACTCTTTGCAGCGGGTGCACACAGCCTGCGCATCCACAATCTGCCGGTACACCGA
  CAGGCGCCGCTGCAGACTCTCGAAAAAGACGTTATCCAGGGCGGGTGTGTCTGCACCTAG
  GGGCCAGGGCGCCTGGCAGATGGGGCACTGCATGCCCGACGCCTCCAGGCATCCTGAGCA
  CAGCGTGTGCAGACAAGGCAGCAGCTTCGGGCACTTGGCTTCCGCCTGGCATTGCTGGCA
  GCGCAGAAACTGGAACTCCTCCTCCGAAGCGGGGGCTCGCTCTGTAGGGCTGGGGCTGGG
  GCTGGGCTGGCGGCCTTCAGAGGGGGTCTCGGGGGGAGGCATGGTGGGCTCCTGGGGCCG
  GGCGGNGTCCTGCTGGGGCCTCGGAGATCGGGCGGGTGCAGGCTCCATGGACCCCAGCTT
  AGTTTCGATTCTCGCTCGTGCCGAATTCGCGGCCGCCCTATAGTGAGTCGTATTACAAAA
  TTCTGACGGTTCACTAAACGAGCTCTGCTTATATAGACCTCCCACCGTACACGCCTACCG
  CCCATTTGCGTCAACGGGGCGGGGTTATTACGACATTTTGGAAAGTCCCGTTGATTTTGG
  TGCCCAAACAAACTCCCATTGACGTCCATGGGGGTGGAGACTTGGAAAATCCCCGTGAGT
  CAAACCGCTATCCACCG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_033244 unedited
  GNANGAGCACTGGGGNAGGGTCACAGGGATGCCACCCGGGTTCTGTTCAGGAAAAGCTAT
  GACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTAAGGCGGACATCTT
  TATTCAAGCCAGCAAATGACACGCCAGGAGCCCAGGTGAGCCGGAGTGCTCTGCCCAGCC
  TGGCTGCAGTTGTTGGCCAGAGCTCAGAGCTCTCCTGGGCCTGGCAGATGGCTCTGCCTG
  CACTTCTTTTTGGGACTCAGAGACTAAATTAGAAAGGGGTGGGGGTAGCCCCAGGAGAAC
  CCACTTTCATTGTCAATCTTGAGGTCAAAGAAAACCAGAGGTCTGTCTTCTGCTTGGGGG
  TCAGCAAGGTTCTCGTCCAGGACCCTGAGGGTGCTGGGGCCTTCCAGCTGGAGGTCACTG
  GACTCACTGCTGCTGTCATCCAGCTCTCGGGAGGACGAGTTTTCGGCATCTGAGTCTTCC
  GAGCTGCTGATCACCACAACGCGTTCCTCTCCCTACCTGCCTCCCCGGCGCCACTGGCCA
  CGTGGTTGCTGTTGGGCAGGAAGACCTCACTTCCTATGACGGGGCTCCTGGGGCTAGGCG
  GTCCATCCAGGTGGGGTGGTGAGACTGCCTTGGAGGTGCTGGGCCTGGGCTGCTCCGGGG
  AGCTCCGAGCCAACCTTGCCTCCTTCCCCTCCTCAGACTCCATCTTGATGACCTTCCTGG
  GGCACTGGGTCTGGCTGCACTTCCTCTTCTGGGCTGTCGTTGTATTGGAGACATCCTCTC
  CATAGGAAGGGCCTTTGATGGAGAAGGCGTACACTGGCACGGGGGTGTGCCCCGGGCACT
  GACTGTACCACAGCCATAGGCTGGGCTTCAGCCN
  
• Restriction Sites: Please inquire
• ACCN: NM_033244
• Insert Size: 2100 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_033244.3, NP_150247.2
• RefSeq Size: 3096 bp
• RefSeq ORF: 1683 bp
• Locus ID: 5371
• Cytogenetics: 15q24.1
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Acute myeloid leukemia, Pathways in cancer, Ubiquitin mediated proteolysis
• Gene Summary: 'The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This phosphoprotein localizes to nuclear bodies where it functions as a transcription factor and tumor suppressor. Its expression is cell-cycle related and it regulates the p53 response to oncogenic signals. The gene is often involved in the translocation with the retinoic acid receptor alpha gene associated with acute promyelocytic leukemia (APL). Extensive alternative splicing of this gene results in several variations of the protein's central and C-terminal regions; all variants encode the same N-terminus. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (5) differs in the 3' UTR and the 3' coding region compared to variant 1. The resulting isoform (5, also known as PML-3B, PML-VI and TRIM19epsilon) contains a distinct C-terminus compared to isoform 1.