TCIRG1 (NM_006019) Human Untagged Clone

CAT#: SC128192

TCIRG1 (untagged)-Human T-cell, immune regulator 1, ATPase, H+ transporting, lysosomal V0 subunit A3 (TCIRG1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TCIRG1
• Synonyms: a3; Atp6i; ATP6N1C; ATP6V0A3; OC-116kDa; OC116; OPTB1; Stv1; TIRC7; Vph1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_006019, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGGCTCCATGTTCCGGAGCGAGGAGGTGGCCCTGGTCCAGCTCTTTCTGCCCACAGCGGCTGCCTACA
  CCTGCGTGAGTCGGCTGGGCGAGCTGGGCCTCGTGGAGTTCAGAGACCTCAACGCCTCGGTGAGCGCCTT
  CCAGAGACGCTTTGTGGTTGATGTTCGGCGCTGTGAGGAGCTGGAGAAGACCTTCACCTTCCTGCAGGAG
  GAGGTGCGGCGGGCTGGGCTGGTCCTGCCCCCGCCAAAGGGGAGGCTGCCGGCACCCCCACCCCGGGACC
  TGCTGCGCATCCAGGAGGAGACGGAGCGCCTGGCCCAGGAGCTGCGGGATGTGCGGGGCAACCAGCAGGC
  CCTGCGGGCCCAGCTGCACCAGCTGCAGCTCCACGCCGCCGTGCTACGCCAGGGCCATGAACCTCAGCTG
  GCAGCCGCCCACACAGATGGGGCCTCAGAGAGGACGCCCCTGCTCCAGGCCCCCGGGGGGCCGCACCAGG
  ACCTGAGGGTCAACTTTGTGGCAGGTGCCGTGGAGCCCCACAAGGCCCCTGCCCTAGAGCGCCTGCTCTG
  GAGGGCCTGCCGCGGCTTCCTCATTGCCAGCTTCAGGGAGCTGGAGCAGCCGCTGGAGCACCCCGTGACG
  GGCGAGCCAGCCACGTGGATGACCTTCCTCATCTCCTACTGGGGTGAGCAGATCGGACAGAAGATCCGCA
  AGATCACGGACTGCTTCCACTGCCACGTCTTCCCGTTTCTGCAGCAGGAGGAGGCCCGCCTCGGGGCCCT
  GCAGCAGCTGCAACAGCAGAGCCAGGAGCTGCAGGAGGTCCTCGGGGAGACAGAGCGGTTCCTGAGCCAG
  GTGCTAGGCCGGGTGCTGCAGCTGCTGCCGCCAGGGCAGGTGCAGGTCCACAAGATGAAGGCCGTGTACC
  TGGCCCTGAACCAGTGCAGCGTGAGCACCACGCACAAGTGCCTCATTGCCGAGGCCTGGTGCTCTGTGCG
  AGACCTGCCCGCCCTGCAGGAGGCCCTGCGGGACAGCTCGATGGAGGAGGGAGTGAGTGCCGTGGCTCAC
  CGCATCCCCTGCCGGGACATGCCCCCCACACTCATCCGCACCAACCGCTTCACGGCCAGCTTCCAGGGCA
  TCGTGGATGCCTACGGCGTGGGCCGCTACCAGGAGGTCAACCCCGCTCCCTACACCATCATCACCTTCCC
  CTTCCTGTTTGCTGTGATGTTCGGGGATGTGGGCCACGGGCTGCTCATGTTCCTCTTCGCCCTGGCCATG
  GTCCTTGCGGAGAACCGACCGGCTGTGAAGGCCGCGCAGAACGAGATCTGGCAGACTTTCTTCAGGGGCC
  GCTACCTGCTCCTGCTTATGGGCCTGTTCTCCATCTACACCGGCTTCATCTACAACGAGTGCTTCAGTCG
  CGCCACCAGCATCTTCCCCTCGGGCTGGAGTGTGGCCGCCATGGCCAACCAGTCTGGCTGGAGTGATGCA
  TTCCTGGCCCAGCACACGATGCTTACCCTGGATCCCAACGTCACCGGTGTCTTCCTGGGACCCTACCCCT
  TTGGCATCGATCCTATTTGGAGCCTGGCTGCCAACCACTTGAGCTTCCTCAACTCCTTCAAGATGAAGAT
  GTCCGTCATCCTGGGCGTCGTGCACATGGCCTTTGGGGTGGTCCTCGGAGTCTTCAACCACGTGCACTTT
  GGCCAGAGGCACCGGCTGCTGCTGGAGACGCTGCCGGAGCTCACCTTCCTGCTGGGACTCTTCGGTTACC
  TCGTGTTCCTAGTCATCTACAAGTGGCTGTGTGTCTGGGCTGCCAGGGCCGCCTCGGCCCCCAGCATCCT
  CATCCACTTCATCAACATGTTCCTCTTCTCCCACAGCCCCAGCAACAGGCTGCTCTACCCCCGGCAGGAG
  GTGGTCCAGGCCACGCTGGTGGTCCTGGCCTTGGCCATGGTGCCCATCCTGCTGCTTGGCACACCCCTGC
  ACCTGCTGCACCGCCACCGCCGCCGCCTGCGGAGGAGGCCCGCTGACCGACAGGAGGAAAACAAGGCCGG
  GTTGCTGGACCTGCCTGACGCATCTGTGAATGGCTGGAGCTCCGATGAGGAAAAGGCAGGGGGCCTGGAT
  GATGAAGAGGAGGCCGAGCTCGTCCCCTCCGAGGTGCTCATGCACCAGGCCATCCACACCATCGAGTTCT
  GCCTGGGCTGCGTCTCCAACACCGCCTCCTACCTGCGCCTGTGGGCCCTGAGCCTGGCCCACGCCCAGCT
  GTCCGAGGTTCTGTGGGCCATGGTGATGCGCATAGGCCTGGGCCTGGGCCGGGAGGTGGGCGTGGCGGCT
  GTGGTGCTGGTCCCCATCTTTGCCGCCTTTGCCGTGATGACCGTGGCTATCCTGCTGGTGATGGAGGGAC
  TCTCAGCCTTCCTGCACGCCCTGCGGCTGCACTGGGTGGAATTCCAGAACAAGTTCTACTCAGGCACGGG
  CTACAAGCTGAGTCCCTTCACCTTCGCTGCCACAGATGACTAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006019 unedited
  GAATTTGTAATACGACTCACTCATAGGGCGGCCGCGAATTCGCACGAGGCGGAGCGGGGC
  AGCCAGCAGCGGAGGCGCGGCGCGCAGCACACCCGGGGACCATGGGCTCCATGTTCCGGA
  GCGAGGAGGTGGCCCTGGTCCAGCTCTTTCTGCCCACAGCGGCTGCCTACACCTGCGTGA
  GTCGGCTGGGCGAGCTGGGCCTCGTGGAGTTCAGAGACCTCAACGCCTCGGTGAGCGCCT
  TCCAGAGACGCTTTGTGGTTGATGTTCGGCGCTGTGAGGAGCTGGAGAAGACCTTCACCT
  TCCTGCAGGAGGAGGTGCGGCGGGCTGGGCTGGTCCTGCCCCCGCCAAAGGGGAGGCTGC
  CGGCACCCCCACCCCGGGACCTGCTGCGCATCCAGGAGGAGACGGAGCGCCTGGCCCAGG
  AGCTGCGGGATGTGCGGGGCAACCAGCAGGCCCTGCGGGCCCAGCTGCACCAGCTGCAGC
  TCCACGCCGCCGTGCTACGCCAGGGCCATGAACCTCAGCTGGCAGCCGCCCACACAGATG
  GGGCCTCAGAGAGGACGCCCCTGCTCCAGGCCCCCGGGGGGCCGCACCANGACCTGAGGG
  TCAACTNTGTGGCATGTGCCGTGGAGCCCCACATGCCCCTGCCCTATAGCGCCTGCTCTG
  GATGGCCTGCCGCGGCTTCCTCATTGCCAGCTTCAGGGAGCTGGAGCAGCCGCTGGAGCA
  CCCCGTGACGATCGGACAGAAGATCCCGCAGATCACGGACTGCTTCCACTGCCACGTCTT
  NCCGTTTCTGCAGCAGGAAGATGCCCGCCTCGGGGCCTGCAGCAGCTGCAACAGCAAAGC
  CAGAGCTGGCANGAGGTCCCTCGGGGAGACAGAGCGGNTCCTGATCCCAGTGCTATGCCN
  GGNTGCTGCATCTGCTGCCGCAGGGNCAGTGCANGTCCACAAATGAAAGGCGTGTACCTG
  CCCCTGACATTGCATGTGAGCACAGCACATTGCCTCATGCGAGCCTGNGCTCTGGCAGAC
  TGCCCGCTGAGAGCN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006019 unedited
  NNNNCCCAACTGTGAACCGCGGCTCGCAATCTAGGATCAGTTTTGTTTTATGGTTTATAT
  TTTTGCCTTTTTTTGTTTTGTTTAGGGCAAAGGCGTACATCTTTATTCCTCTCCTGACTC
  AAGAGTCAAGAAAGAGGTCTGGCAGGACCTGCAGTGGGCCCTAATCAACTGTGGCACCGA
  AAGTGAAAGGACTCAACTTGTGACCCGTGCCTGAATAAAACTTGGTCTGGAATTCCACCC
  CTTGCAACCCCAGGGCGTGCAAGAAGGCTGAAAATCCCTCCATCACCAGCCGGATAACCA
  CGGGCATTACGGCTAAAGCCGCAAAAATGGGGACCAGTACCACAAGCGCCAAGCCCACCT
  CCCGGCCCAGGGCCAAGCCTATGCGCATCACCATGGCCCACAAAACCTCGGACAACTGGG
  CGTGGGCCAAGCTCAAGGCCCACAAGCACAAGNCAAGACGCTGTGTTGGAGACTCAGCCC
  AACAAAACTTCAAGGTGTGGATTGCCTGTGCATGAGCACCTCGGGAGGGGAGACAAGCTG
  GGGCTTCTTGTTATTATCCAAGCCCCCTGGTNNNTCTTTCCTATGGAGCCCCGGGCCTTT
  TCCAAAATGGTAAGGAAGGGTCCCGCACCCCGGCCCTGTTTTCCCCCCCGCGGGCAAAGG
  GCCCCCCTCCCAAAAAGGGGTTTAATGGAGGTTCCACACAGTTCCAGGTTTTCCCCCAAT
  TCTTGTTGGGGCCCCATGGCAAAGGCCCGAACCCCACAGGGGGGCCGGGGCCCACCCCCC
  CCCGGGGGGAGAAAAAACCCTTTTTTCGGGGGTTTGTGGCAAAAAAAAAAAACCTTTTAA
  AAAAAGGGGTAAGAAATCCCCGGGGCCCCCAGGGGGGCCTTTTAACCCCCATAAAACAGA
  GCCCTTTTTG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_006019
• ORF Size: 2493 bp
• Insert Size: 2700
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_006019.2, NP_006010.2
• RefSeq Size: 2700
• RefSeq ORF: 2493
• Locus ID: 10312
• Domains: V_ATPase_sub_a
• Protein Families: Transmembrane
• Protein Pathways: Epithelial cell signaling in Helicobacter pylori infection, Lysosome, Metabolic pathways, Oxidative phosphorylation, Vibrio cholerae infection
• Gene Summary: This gene encodes a subunit of a large protein complex known as a vacuolar H+-ATPase (V-ATPase). The protein complex acts as a pump to move protons across the membrane. This movement of protons helps regulate the pH of cells and their surrounding environment. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, and receptor-mediated endocytosis. V-ATPase is comprised of a cytosolic V1 domain and a transmembrane V0 domain. Alternative splicing results in multiple transcript variants. Mutations in this gene are associated with infantile malignant osteopetrosis. [provided by RefSeq, May 2017]
  Transcript Variant: This variant (1) encodes isoform a, also known as the OC116 protein because it is one of three V-ATPase 116 kD subunits. It is also referred to as the a3 isoform and is induced on osteoclast differentiation. This variant contains a unique 5' end and lacks an internal segment compared to variant 2, which results in an isoform (a) with a different N-terminus than that of isoform b.