DHODH (NM_001361) Human Untagged Clone

CAT#: SC128197

DHODH (untagged)-Human dihydroorotate dehydrogenase (DHODH), nuclear gene encoding mitochondrial protein


  "NM_001361" in other vectors (5)

Reconstitution Protocol

USD 310.00

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol DHODH
Synonyms DHOdehase; POADS; URA1
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC128197 sequence for NM_001361 edited (data generated by NextGen Sequencing)
ATGGCGTGGAGACACCTGCAAAAGCGGGCCCAGGATGCTGTGATCATCCTGGGGGGAGGA
GGACTTCTCTTCGCCTCCTACCTGATGGCCACGGGAGATGAGCGTTTCTATGCTGAACAC
CTGATGCCGACTCTGCAGGGGCTGCTGGACCCGGAGTCAGCCCACAGACTGGCTGTTCGC
TTCACCTCCCTGGGGCTCCTTCCACGGGCCAGATTTCAAGACTCTGACATGCTGGAAGTG
AGAGTTCTGGGCCATAAATTCCGAAATCCAGTAGGAATTGCTGCAGGATTTGACAAGCAT
GGGGAAGCCGTGGACGGACTTTATAAGATGGGCTTTGGTTTTGTTGAGATAGGAAGTGTG
ACTCCAAAACCTCAGGAAGGAAACCCTAGACCCAGAGTCTTCCGCCTCCCTGAGGACCAA
GCTGTCATTAACAGGTATGGATTTAACAGTCACGGGCTTTCAGTGGTGGAACACAGGTTA
CGGGCCAGACAGCAGAAGCAGGCCAAGCTCACAGAAGATGGACTGCCTCTGGGGGTCAAC
TTGGGGAAGAACAAGACCTCAGTGGACGCCGCGGAGGACTACGCAGAAGGGGTGCGCGTA
CTGGGCCCCCTGGCCGACTACCTGGTGGTGAATGTGTCCAGCCCCAACACTGCCGGGCTG
CGGAGCCTTCAGGGAAAGGCCGAGCTGCGCCGCCTGCTGACCAAGGTGCTGCAGGAGAGG
GATGGCTTGCGGAGAGTGCACAGGCCGGCAGTCCTGGTGAAGATCGCTCCTGACCTCACC
AGCCAGGATAAGGAGGACATTGCCAGTGTGGTCAAAGAGTTGGGCATCGATGGGCTGATT
GTTACGAACACCACCGTGAGTCGCCCTGCGGGCCTCCAGGGTGCCCTGCGCTCTGAAACA
GGAGGGCTGAGTGGGAAGCCCCTCCGGGATTTATCAACTCAAACCATTCGGGAGATGTAT
GCACTCACCCAAGGCCGAGTTCCCATAATTGGGGTTGGTGGTGTGAGCAGCGGGCAGGAC
GTGCTGGAGAAGATCCGGGCAGGGGCCTCCCTGGTGCAGCTGTACACGGCCCTCACCTTC
TGGGGGCCACCCGTTGTGGGCAAAGTCAAGCGGGAACTGGAGGCCCTTCTGAAAGAGCAG
GGCTTTGGCGGAGTCACAGATGCCATTGGAGCAGATCATCGGAGGTGA

Clone variation with respect to NM_001361.4
19 a=>c;1022 c=>t
>OriGene 5' read for NM_001361 unedited
GACTCACTATAGGGCGGCCGCGAATTCGGCACCAGATGGCGTGGAGACACCTGCAAAAGC
GGGCCCAGGATGCTGTGATCATCCTGGGGGGAGGAGGACTTCTCTTCGCCTCCTACCTGA
TGGCCACGGGAGATGAGCGTTTCTATGCTGAACACCTGATGCCGACTCTGCAAGGGCTGC
TGGACCCGGAGTCAACCCACAAACTGGCTGTTCGCTTCACCTTCCTGGGGCTCCTTTCAC
GGGCCAAATTTCAAGACTCTGACATGCTGGAAGTGAGAGTTCTGGGCCATAAATTCCGAA
ATCCAGTAAGAATTGCTGCAGGATTTGACAAGCATGGGGAAACCGTGGACCGACTTTATA
AGATGGGCTTTGGTTTTGTTGAGATAGGAAGTGTGACTCCAAAACCTCAGGAAGGAAACC
CTAGACCCAGAGTCTTCCGCCTTCCTGAAGACCAAGCTGTCATTAACAGGTATGGATTTA
ACAGTCACGGGCTTTCAGTGGTGGAACACAAGTTACCGGCCAAACAGCAGAAGCAGGCCA
AGCTCACAGAAGATGGACTGGCTCTGGGGGTCAACTTGGGGAAGAACAAGACCTCAGTGG
ACGCCGCGGAGGACTACGCACAAAGGGTGCGCGTACTGGGCCCCCTGGGCGACTACCTGG
TGGTGAATGTGTGCAGACCCAACACTGCCGGGCTGCCGAGCCTCTAAGGAAAGGCCGAGC
TGCGCCCGCTGCTTG
>OriGene 3' read for NM_001361 unedited
CCGGCCAGGAGAGGCACTGGGGAGGGGTCACAGGGATGCCACCCGGGATCTGTTCAGGAA
ACAGCTATGACCTGTTGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTT
TTTTTTTTTTTTTTTTTTTTTTTTTTCCCAGGTTCTAAATGGCTTTATTTTTTCCATGAC
TTTTTCCTCCTAATATTCAATGTCCTTGCAAGACTGATGGATCCTAGGGTTTGAATCCAC
AAAGAATCAAAAATGCAGTTTATGGTCTGACCAAGGGACTGAAAAAAGCCTGCGGTTGAT
CCTGGTGACCCCCCGATTGGCGTTTACAGTGCAGCCATGCCATGGCTGGGGGACATGGCT
CAAGATGGAGTCCCTCCTCTCATGATCCAGCCACAAAGGCTTGCCTGAGTCCTTGGGAAG
GTTCCAGATCAGGCTTCCCGTCAGACGCTGTCCTCACCTCCGATGATCTGCTCCAATGGC
ATCTGTGACTCCGCCAAAGCCCTGCTCTTTCAGAAGGGCCTCCAGTTCCCGCTTGACTTT
GCCCACAACGGGTGGCCCCCAAAAGGTGAGGGCCGTTACAGCTGCACAGGGAGGCCCTTG
CCCGTTTTTTTTCCAACATTCTTGCCGTTGTTACCCACCACCCCAATTTTGGAACTCGCC
TGGGTGATGCTACTCTCCAATGGTTGAGTTATAATCCCGAGGGCTTCCCTCACCCT
Restriction Sites ECoRI-NOT     
ACCN NM_001361
Insert Size 1700 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001361.3, NP_001352.2
RefSeq Size 2080 bp
RefSeq ORF 1188 bp
Locus ID 1723
Cytogenetics 16q22.2
Protein Families Druggable Genome, Transmembrane
Protein Pathways Metabolic pathways, Pyrimidine metabolism
Gene Summary 'The protein encoded by this gene catalyzes the fourth enzymatic step, the ubiquinone-mediated oxidation of dihydroorotate to orotate, in de novo pyrimidine biosynthesis. This protein is a mitochondrial protein located on the outer surface of the inner mitochondrial membrane. [provided by RefSeq, Jul 2008]'

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