DHODH (NM_001361) Human Untagged Clone

CAT#: SC128197

DHODH (untagged)-Human dihydroorotate dehydrogenase (DHODH), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DHODH
• Synonyms: DHOdehase; POADS; URA1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC128197 sequence for NM_001361 edited (data generated by NextGen Sequencing)
  ATGGCGTGGAGACACCTGCAAAAGCGGGCCCAGGATGCTGTGATCATCCTGGGGGGAGGA
  GGACTTCTCTTCGCCTCCTACCTGATGGCCACGGGAGATGAGCGTTTCTATGCTGAACAC
  CTGATGCCGACTCTGCAGGGGCTGCTGGACCCGGAGTCAGCCCACAGACTGGCTGTTCGC
  TTCACCTCCCTGGGGCTCCTTCCACGGGCCAGATTTCAAGACTCTGACATGCTGGAAGTG
  AGAGTTCTGGGCCATAAATTCCGAAATCCAGTAGGAATTGCTGCAGGATTTGACAAGCAT
  GGGGAAGCCGTGGACGGACTTTATAAGATGGGCTTTGGTTTTGTTGAGATAGGAAGTGTG
  ACTCCAAAACCTCAGGAAGGAAACCCTAGACCCAGAGTCTTCCGCCTCCCTGAGGACCAA
  GCTGTCATTAACAGGTATGGATTTAACAGTCACGGGCTTTCAGTGGTGGAACACAGGTTA
  CGGGCCAGACAGCAGAAGCAGGCCAAGCTCACAGAAGATGGACTGCCTCTGGGGGTCAAC
  TTGGGGAAGAACAAGACCTCAGTGGACGCCGCGGAGGACTACGCAGAAGGGGTGCGCGTA
  CTGGGCCCCCTGGCCGACTACCTGGTGGTGAATGTGTCCAGCCCCAACACTGCCGGGCTG
  CGGAGCCTTCAGGGAAAGGCCGAGCTGCGCCGCCTGCTGACCAAGGTGCTGCAGGAGAGG
  GATGGCTTGCGGAGAGTGCACAGGCCGGCAGTCCTGGTGAAGATCGCTCCTGACCTCACC
  AGCCAGGATAAGGAGGACATTGCCAGTGTGGTCAAAGAGTTGGGCATCGATGGGCTGATT
  GTTACGAACACCACCGTGAGTCGCCCTGCGGGCCTCCAGGGTGCCCTGCGCTCTGAAACA
  GGAGGGCTGAGTGGGAAGCCCCTCCGGGATTTATCAACTCAAACCATTCGGGAGATGTAT
  GCACTCACCCAAGGCCGAGTTCCCATAATTGGGGTTGGTGGTGTGAGCAGCGGGCAGGAC
  GTGCTGGAGAAGATCCGGGCAGGGGCCTCCCTGGTGCAGCTGTACACGGCCCTCACCTTC
  TGGGGGCCACCCGTTGTGGGCAAAGTCAAGCGGGAACTGGAGGCCCTTCTGAAAGAGCAG
  GGCTTTGGCGGAGTCACAGATGCCATTGGAGCAGATCATCGGAGGTGA
  
  Clone variation with respect to NM_001361.4
  19 a=>c;1022 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001361 unedited
  GACTCACTATAGGGCGGCCGCGAATTCGGCACCAGATGGCGTGGAGACACCTGCAAAAGC
  GGGCCCAGGATGCTGTGATCATCCTGGGGGGAGGAGGACTTCTCTTCGCCTCCTACCTGA
  TGGCCACGGGAGATGAGCGTTTCTATGCTGAACACCTGATGCCGACTCTGCAAGGGCTGC
  TGGACCCGGAGTCAACCCACAAACTGGCTGTTCGCTTCACCTTCCTGGGGCTCCTTTCAC
  GGGCCAAATTTCAAGACTCTGACATGCTGGAAGTGAGAGTTCTGGGCCATAAATTCCGAA
  ATCCAGTAAGAATTGCTGCAGGATTTGACAAGCATGGGGAAACCGTGGACCGACTTTATA
  AGATGGGCTTTGGTTTTGTTGAGATAGGAAGTGTGACTCCAAAACCTCAGGAAGGAAACC
  CTAGACCCAGAGTCTTCCGCCTTCCTGAAGACCAAGCTGTCATTAACAGGTATGGATTTA
  ACAGTCACGGGCTTTCAGTGGTGGAACACAAGTTACCGGCCAAACAGCAGAAGCAGGCCA
  AGCTCACAGAAGATGGACTGGCTCTGGGGGTCAACTTGGGGAAGAACAAGACCTCAGTGG
  ACGCCGCGGAGGACTACGCACAAAGGGTGCGCGTACTGGGCCCCCTGGGCGACTACCTGG
  TGGTGAATGTGTGCAGACCCAACACTGCCGGGCTGCCGAGCCTCTAAGGAAAGGCCGAGC
  TGCGCCCGCTGCTTG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001361 unedited
  CCGGCCAGGAGAGGCACTGGGGAGGGGTCACAGGGATGCCACCCGGGATCTGTTCAGGAA
  ACAGCTATGACCTGTTGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTTTTTTTTCCCAGGTTCTAAATGGCTTTATTTTTTCCATGAC
  TTTTTCCTCCTAATATTCAATGTCCTTGCAAGACTGATGGATCCTAGGGTTTGAATCCAC
  AAAGAATCAAAAATGCAGTTTATGGTCTGACCAAGGGACTGAAAAAAGCCTGCGGTTGAT
  CCTGGTGACCCCCCGATTGGCGTTTACAGTGCAGCCATGCCATGGCTGGGGGACATGGCT
  CAAGATGGAGTCCCTCCTCTCATGATCCAGCCACAAAGGCTTGCCTGAGTCCTTGGGAAG
  GTTCCAGATCAGGCTTCCCGTCAGACGCTGTCCTCACCTCCGATGATCTGCTCCAATGGC
  ATCTGTGACTCCGCCAAAGCCCTGCTCTTTCAGAAGGGCCTCCAGTTCCCGCTTGACTTT
  GCCCACAACGGGTGGCCCCCAAAAGGTGAGGGCCGTTACAGCTGCACAGGGAGGCCCTTG
  CCCGTTTTTTTTCCAACATTCTTGCCGTTGTTACCCACCACCCCAATTTTGGAACTCGCC
  TGGGTGATGCTACTCTCCAATGGTTGAGTTATAATCCCGAGGGCTTCCCTCACCCT
  
• Restriction Sites: ECoRI-NOT
• ACCN: NM_001361
• Insert Size: 1700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001361.3, NP_001352.2
• RefSeq Size: 2080 bp
• RefSeq ORF: 1188 bp
• Locus ID: 1723
• Cytogenetics: 16q22.2
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Metabolic pathways, Pyrimidine metabolism
• Gene Summary: 'The protein encoded by this gene catalyzes the fourth enzymatic step, the ubiquinone-mediated oxidation of dihydroorotate to orotate, in de novo pyrimidine biosynthesis. This protein is a mitochondrial protein located on the outer surface of the inner mitochondrial membrane. [provided by RefSeq, Jul 2008]'