M-CSF (CSF1) (NM_000757) Human Untagged Clone

CAT#: SC128208

CSF1 (untagged)-Human colony stimulating factor 1 (macrophage) (CSF1), transcript variant 1


  "NM_000757" in other vectors (4)

Reconstitution Protocol

USD 310.00

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol CSF1
Synonyms CSF-1; MCSF
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC128208 sequence for NM_000757 edited (data generated by NextGen Sequencing)
ATGACCGCGCCGGGCGCCGCCGGGCGCTGCCCTCCCACGACATGGCTGGGCTCCCTGCTG
TTGTTGGTCTGTCTCCTGGCGAGCAGGAGTATCACCGAGGAGGTGTCGGAGTACTGTAGC
CACATGATTGGGAGTGGACACCTGCAGTCTCTGCAGCGGCTGATTGACAGTCAGATGGAG
ACCTCGTGCCAAATTACATTTGAGTTTGTAGACCAGGAACAGTTGAAAGATCCAGTGTGC
TACCTTAAGAAGGCATTTCTCCTGGTACAAGACATAATGGAGGACACCATGCGCTTCAGA
GATAACACCCCCAATGCCATCGCCATTGTGCAGCTGCAGGAACTCTCTTTGAGGCTGAAG
AGCTGCTTCACCAAGGATTATGAAGAGCATGACAAGGCCTGCGTCCGAACTTTCTATGAG
ACACCTCTCCAGTTGCTGGAGAAGGTCAAGAATGTCTTTAATGAAACAAAGAATCTCCTT
GACAAGGACTGGAATATTTTCAGCAAGAACTGCAACAACAGCTTTGCTGAATGCTCCAGC
CAAGATGTGGTGACCAAGCCTGATTGCAACTGCCTGTACCCCAAAGCCATCCCTAGCAGT
GACCCGGCCTCTGTCTCCCCTCATCAGCCCCTCGCCCCCTCCATGGCCCCTGTGGCTGGC
TTGACCTGGGAGGACTCTGAGGGAACTGAGGGCAGCTCCCTCTTGCCTGGTGAGCAGCCC
CTGCACACAGTGGATCCAGGCAGTGCCAAGCAGCGGCCACCCAGGAGCACCTGCCAGAGC
TTTGAGCCGCCAGAGACCCCAGTTGTCAAGGACAGCACCATCGGTGGCTCACCACAGCCT
CGCCCCTCTGTCGGGGCCTTCAACCCCGGGATGGAGGATATTCTTGACTCTGCAATGGGC
ACTAATTGGGTCCCAGAAGAAGCCTCTGGAGAGGCCAGTGAGATTCCCGTACCCCAAGGG
ACAGAGCTTTCCCCCTCCAGGCCAGGAGGGGGCAGCATGCAGACAGAGCCCGCCAGACCC
AGCAACTTCCTCTCAGCATCTTCTCCACTCCCTGCATCAGCAAAGGGCCAACAGCCGGCA
GATGTAACTGGTACAGCCTTGCCCAGGGTGGGCCCCGTGAGGCCCACTGGCCAGGACTGG
AATCACACCCCCCAGAAGACAGACCATCCATCTGCCCTGCTCAGAGACCCCCCGGAGCCA
GGCTCTCCCAGGATCTCATCACTGCGCCCCCAGGGCCTCAGCAACCCCTCCACCCTCTCT
GCTCAGCCACAGCTTTCCAGAAGCCACTCCTCGGGCAGCGTGCTGCCCCTTGGGGAGCTG
GAGGGCAGGAGGAGCACCAGGGATCGGAGGAGCCCCGCAGAGCCAGAAGGAGGACCAGNN
NNNNNNNNNGCAGCCAGGCCCCTGCCCCGTTTTAACTCCGTTCCTTTGACTGACACAGGC
CATGAGAGGCAGTCCGAGGGATCCTCCAGCCCGCAGCTCCAGGAGTCTGTCTTCCACCTG
CTGGTGCCCAGTGTCATCCTGGTCTTGCTGGCCGTCGGAGGCCTCTTGTTCTACAGGTGG
AGGCGGCGGAGCCATCAAGAGCCTCAGAGAGCGGATTCTCCCTTGGAGCAACCAGAGGGC
AGCCCCCTGACTCAGGATGACAGACAGGTGGAACTGCCAGTGTAG

Clone variation with respect to NM_000757.5
1095 c=>a;1379 c=>n;1380 a=>n;1381 a=>n;1382 g=>n;1383 t=>n;1384 g=>n;1385 a=>n;1386 a=>n;1387 g=>n;1388 g=>n;1389 g=>n
>OriGene 5' read for NM_000757 unedited
GTTCGGATTTGTATACGACTTATATAGGGCGGCCGCGAATTCGCACGAGGGAGGGCTGGC
CAGTGAGGCTCGGCCCGGGGAAAGTGAAAGTTTGCCTGGGTCCTCTCGGCGCCAGAGCCG
CTCTCCGCATCCCAGGACAGCGGTGCGGCCCTCGGCCGGGGCGCCCACTCCGCAGCAGCC
AGCGAGCGAGCGAGCGAGCGAGGGCGGCCGACGCGCCCGGCCGGGACCCAGCTGCCCGTA
TGACCGCGCCGGGCGCCGCCGGGCGCTGCCCTCCCACGACATGGCTGGGCTCCCTGCTGT
TGTTGGTCTGTCTCCTGGCGAGCAGGAGTATCACCGAGGAGGTGTCGGAGTACTGTAGCC
ACATGATTGGGAGTGGACACCTGCAGTCTCTGCAGCGGCTGATTGACAGTCAGATGGAGA
CCTCGTGCCAAATTACATTTGAGTTTGTAGACCAGGAACAGTTGAAAGATCCAGTGTGCT
ACCTTAAGAAGGCATTTCTCCTGGTACAAGACATAATGGAGGGACACCATGCGCTTCAGA
GATAACACCCCCAATGCCATCGCCATTGTGCAGCTGCAGGAACTCTCTTTGAGGCTGGAA
GAGCTGCTTCACCAAGGATTATGAAGAGCATGACAAGGCCTGCGTCCGAACTTCCTATGG
AGACACCTCTCCATTTGCTGGAGAAGGTCAAGAATGTCTTTAATGAAACAAAGAATCTCC
TTGACAGGGACTGGGAATATTTTCAGCAAGAACTGCAACAACAGCCTTGCTGAGTGCTCC
ACCCAGAAGTGGTGACCAAGCCTGATGGCAATGCCTGTGCCCCAAAACCATCCCTAGCAG
TGACCCGGCCTCTGGGTTCCCTAATAAGCCCCTTGCCCCCTCCTGGGGCCTGGGGGCTGG
GCTGACCGGGGAGGACTCTGAGGGAACG
>OriGene 3' read for NM_000757 unedited
AGAGCTGGNATAGTGGAGACGGTCGGTTGCTGGCTCATGGATGGACCGGGGCAGGCTGTT
CAGCAAGCAGGTACAGTGTTCACAGTGCAATGGCTTCCTTCATGAGGGGAGCCTCTGCTT
GGCCTCCTGGAGAAGCTCTGGCAGGTGCTGGAAGGAGGAGGGGCAGAGGCCTGTGCTTGA
TCCCCCTCTTGGCATCACTGGCAGGTCAGGCAGGTCCTGAGGGCAGGGCTGGCCTCCCCT
CTGCGCCTGGCAACCAGCACAGGGCACTTATGTGAGCCGAGGGTGTAGACCCTTCAGGCT
GTCAGTGCAGACCAGCAGGTCCAGAGGCCTCCTCTTCCCGTCTCTCAGGCTGCACACCTT
TACGGGAAATCAGGCCGACGCTGTCCCGCCCCACGAACTTCAGGCTCCTCTCTCTGGAGA
CAGGTCCATGCCTGTCCAGGCTGGGATACCTTGGCACCAGGCAGGCCCTCTGGGTGCAGG
GTTCTTTCACTCCTCAGCGTCCACCCTGCTTTCCCACAAACCACAAGCCGGTGGGTCCCT
GGGCCTGGGTTCCGGCATGGAAGGGAGTGAGAGGAGGGTGCAGCCCCTGAGGGAAGAGGG
TCCTGAGCCGGGGTCACCAACCTTTCTCAAGCACATGGGGAGCCTCCCAGGGCTCACAAT
AAATAGTGGCACCCCTGGGGAGCATCCCTCTGGGCTGTTTTCTTTCATTCAAAGGGTCTG
CCGGGTTGGGACAAAAGGGCCCCCAGCCCGGCCTGCCCTGGTCCCAATCCCTGGAAGCCA
GTACCTCTGGGTGAGGGGCAAACCACATTCTCAGAGA
Restriction Sites Please inquire     
ACCN NM_000757
Insert Size 3000 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_000757.3, NP_000748.3
RefSeq Size 2749 bp
RefSeq ORF 1665 bp
Locus ID 1435
Cytogenetics 1p13.3
Protein Families Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein, Transmembrane
Protein Pathways Cytokine-cytokine receptor interaction, Hematopoietic cell lineage
Gene Summary 'The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of macrophages. The active form of the protein is found extracellularly as a disulfide-linked homodimer, and is thought to be produced by proteolytic cleavage of membrane-bound precursors. The encoded protein may be involved in development of the placenta. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2011]'
Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. CCDS Note: This CCDS representation uses a downstream in-frame AUG start codon, which is supported by homology and publication data. The use of the 5'-most in-frame AUG in the transcript would result in a 74 aa N-terminal extension, but this N-terminal extension is not conserved in other species, and it would eliminate an N-terminal signal peptide, which is known to be present based on experimental evidence (PMIDs 8262907, 3498652).

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