MCM7 (NM_005916) Human Untagged Clone

CAT#: SC128224

MCM7 (untagged)-Human minichromosome maintenance complex component 7 (MCM7), transcript variant 1


  "NM_005916" in other vectors (6)

Reconstitution Protocol

USD 310.00

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol MCM7
Synonyms CDC47; MCM2; P1.1-MCM3; P1CDC47; P85MCM; PNAS146; PPP1R104
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC128224 sequence for NM_005916 edited (data generated by NextGen Sequencing)
ATGGCACTGAAGGACTACGCGCTAGAGAAGGAAAAGGTTAAGAAGTTCTTACAAGAGTTC
TACCAGGATGATGAACTCGGGAAGAAGCAGTTCAAGTATGGGAACCAGTTGGTTCGGCTG
GCTCATCGGGAACAGGTGGCTCTGTATGTGGACCTGGACGACGTAGCCGAGGATGACCCC
GAGTTGGTGGACTCAATTTGTGAGAATGCCAGGCGCTACGCGAAGCTCTTTGCTGATGCC
GTACAAGAGCTGCTGCCTCAGTACAAGGAGAGGGAAGTGGTAAATAAAGATGTCCTGGAC
GTTTACATTGAGCATCGGCTAATGATGGAGCAGCGGAGTCGGGACCCTGGGATGGTCCGA
AGCCCCCAGAACCAGTACCCTGCTGAACTCATGCGCAGATTTGAGCTGTATTTTCAAGGC
CCTAGCAGCAACAAGCCTCGTGTGATCCGGGAAGTGCGGGCTGACTCTGTGGGGAAGTTG
GTAACTGTGCGTGGAATCGTCACTCGTGTCTCTGAAGTCAAACCCAAGATGGTGGTGGCC
ACTTACACTTGTGACCAGTGTGGGGCAGAGACCTACCAGCCGATCCAGTCTCCCACTTTC
ATGCCTCTGATCATGTGCCCAAGCCAGGAGTGCCAAACCAACCGCTCAGGAGGGCGGCTG
TATCTGCAGACACGGGGCTCCAGATTCATCAAATTCCAGGAGATGAAGATGCAAGAACAT
AGTGATCAGGTGCCTGTGGGAAATATCCCTCGTAGTATCACGGTGCTGGTAGAAGGAGAG
AACACAAGGATTGCCCAGCCTGGAGACCACGTCAGCGTCACTGGTATTTTCTTGCCAATC
CTGCGCACTGGGTTCCGACAGGTGGTACAGGGTTTACTCTCAGAAACCTACCTGGAAGCC
CATCGGATTGTGAAGATGAACAAGAGTGAGGATGATGAGTCTGGGGCTGGAGAGCTCACC
AGGGAGGAGCTGAGGCAAATTGCAGAGGAGGATTTCTACGAAAAGCTGGCAGCTTCAATC
GCCCCAGAAATATACGGGCATGAAGATGTGAAGAAGGCACTGCTGCTCCTGCTAGTCGGG
GGTGTGGACCAGTCTCCTCGAGGCATGAAAATCCGGGGCAACATCAACATCTGTCTGATG
GGGGATCCTGGTGTGGCCAAGTCTCAGCTCCTGTCATACATTGATCGACTGGCGCCTCGC
AGCCAGTACACAACAGGCCGGGGCTCCTCAGGAGTGGGGCTTACGGCAGCTGTGCTGAGA
GACTCCGTGAGTGGAGAACTGACCTTAGAGGGTGGGGCCCTGGTGCTGGCTGACCAGGGT
GTGTGCTGCATTGATGAGTTCGACAAGATGGCTGAGGCCGACCGCACAGCCATCCACGAG
GTCATGGAGCAGCAGACCATCTCCATTGCCAAGGCCGGCATTCTCACCACACTCAATGCC
CGCTGCTCCATCCTGGCTGCCGCCAACCCTGCCTACGGGCGCTACAACCCTCGCCGCAGC
CTGGAGCAGAACATACAGCTACCTGCTGCACTGCTCTCCCGGTTTGACCTCCTCTGGCTG
ATTCAGGACCGGCCCGACCGAGACAATGACCTACGGTTGGCCCAGCACATTACCTATGTG
CACCAGCACAGCCGGCAGCCCCCCTCCCAGTTTGAACCTCTGGACATGAAGCTCATGAGG
CGTTACATAGCCATGTGCCGCGAGAAGCAGCCCATGGTGCCAGAGTCTCTGGCTGACTAC
ATCACAGCAGCATACGTGGAGATGAGGCGAGAGGCTTGGGCTAGTAAGGATGCCACCTAT
ACTTCTGCCCGGACCCTGCTGGCTATCCTGCGCCTTTCCACTGCTCTGGCACGTCTGAGA
ATGGTGGATGTGGTGGAGAAAGAAGATGTGAATGAAGCCATCAGGCTAATGGAGATGTCA
AAGGACTCTCTTCTAGGAGACAAGGGGCAGACAGCTAGGACTCAGAGACCAGCAGATGTG
ATATTTGCCACCGTCCGTGAACTGGTCTCAGGGGGCCGAAGTGTCCGGTTCTCTGAGGCA
GAGCAGCGCTGTGTATCTCGTGGCTTCACACCCGCCCAGTTCCAGGCGGCTCTGGATGAA
TATGAGGAGCTCAATGTCTGGCAGGTCAATGCTTCCCGGACACGGATCACTTTTGTCTGA

Clone variation with respect to NM_005916.3
1611 c=>t
>OriGene 5' read for NM_005916 unedited
TCAGTATATTTGTATACGACTCACTATAGGGCGGCCCGCGAATTCGCACGAGGTGCGCTG
GCCCTTTAAGAGCGATTCTCCTCCGCCCGCGCCAGCTCGGACCGCGGGAAACCCGGCGCC
TGCACTACCCCGCCCGGAGATTCCCTTCCGACGCCCGCACCGCCTCCCCGTCACTCATTC
TAGGCCCGCACGGTGATTGGCTTGCGGCTAGCGGGAGGTGAAGAAGGCCGCCTTGTCCGA
TTGGCCCGCACGCAGTGGCGCCGGTCACGTGGGGGGCGACGTTTCGCGCCAATTTCGGTT
GGCCGGCCACAGTCCACCGCGCGGAGATTCTCAGCTTCCCCAGGAGCAAGACCTCTGAGC
CCGCCAAGCGCGGCCGCACGGCCCTCGGCAGCGATGGCACTGAAGGACTACGCGCTAGAG
AAGGAAAAGGTTAAGAAGTTCTTACAAGAGTTCTACCAGGATGATGAACTCGGGAAGAAG
CAGTTCAAGTATGGGAACCAGTTGGTTCGGCTGGCTCATCGGGAACAGGTGGCTCTGTAT
GTGGACCTGGACGACGTANCCCGAGATGACCCCGAGTTGGTGGACTCAATTTGTGAGAAT
GCCAGGCGCTACGCGAAGCTCTTTGCTGATGCCGTACAAGAGCTGCTGCCTCAGTCCAGG
AGAGGGAAGTGTAAATAAAGATGCCTGGACGTTACATTGAGCATCGGCTATGATGGACAN
NCGAGTCCAACCCTGGGATGGTCCGAGCCCCCAAACCAGTACCCTGCTGAACTCTGCGCA
GATTGAGCTGTATTTTCAAGGCCTAACAGCACAGCCTCGTGTGATCCGGGAGTGCGGCTG
ACTTGGGGGGAAGTGGTACTGTGCGTGAATCGNACTCGTGCTCTGAGTCAACCCAGATGG
GGTGGCACCTAACTGGGACCGTGTGGGCAAAACTACAGCGATCAGNTCCCTTTATGCTTT
GAATGGGCAGNCAGATGCC
Restriction Sites NotI-NotI     
ACCN NM_005916
Insert Size 2650 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_005916.3, NP_005907.3
RefSeq Size 2821 bp
RefSeq ORF 2160 bp
Locus ID 4176
Cytogenetics 7q22.1
Domains MCM, AAA
Protein Families Transcription Factors
Protein Pathways Cell cycle, DNA replication
Gene Summary 'The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, and may regulate the binding of this protein with the tumorsuppressor protein RB1/RB. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]'
Transcript Variant: This variant (1) encodes the longer isoform (1).

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