MCM7 (NM_005916) Human Untagged Clone

CAT#: SC128224

MCM7 (untagged)-Human minichromosome maintenance complex component 7 (MCM7), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MCM7
• Synonyms: CDC47; MCM2; P1.1-MCM3; P1CDC47; P85MCM; PNAS146; PPP1R104
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC128224 sequence for NM_005916 edited (data generated by NextGen Sequencing)
  ATGGCACTGAAGGACTACGCGCTAGAGAAGGAAAAGGTTAAGAAGTTCTTACAAGAGTTC
  TACCAGGATGATGAACTCGGGAAGAAGCAGTTCAAGTATGGGAACCAGTTGGTTCGGCTG
  GCTCATCGGGAACAGGTGGCTCTGTATGTGGACCTGGACGACGTAGCCGAGGATGACCCC
  GAGTTGGTGGACTCAATTTGTGAGAATGCCAGGCGCTACGCGAAGCTCTTTGCTGATGCC
  GTACAAGAGCTGCTGCCTCAGTACAAGGAGAGGGAAGTGGTAAATAAAGATGTCCTGGAC
  GTTTACATTGAGCATCGGCTAATGATGGAGCAGCGGAGTCGGGACCCTGGGATGGTCCGA
  AGCCCCCAGAACCAGTACCCTGCTGAACTCATGCGCAGATTTGAGCTGTATTTTCAAGGC
  CCTAGCAGCAACAAGCCTCGTGTGATCCGGGAAGTGCGGGCTGACTCTGTGGGGAAGTTG
  GTAACTGTGCGTGGAATCGTCACTCGTGTCTCTGAAGTCAAACCCAAGATGGTGGTGGCC
  ACTTACACTTGTGACCAGTGTGGGGCAGAGACCTACCAGCCGATCCAGTCTCCCACTTTC
  ATGCCTCTGATCATGTGCCCAAGCCAGGAGTGCCAAACCAACCGCTCAGGAGGGCGGCTG
  TATCTGCAGACACGGGGCTCCAGATTCATCAAATTCCAGGAGATGAAGATGCAAGAACAT
  AGTGATCAGGTGCCTGTGGGAAATATCCCTCGTAGTATCACGGTGCTGGTAGAAGGAGAG
  AACACAAGGATTGCCCAGCCTGGAGACCACGTCAGCGTCACTGGTATTTTCTTGCCAATC
  CTGCGCACTGGGTTCCGACAGGTGGTACAGGGTTTACTCTCAGAAACCTACCTGGAAGCC
  CATCGGATTGTGAAGATGAACAAGAGTGAGGATGATGAGTCTGGGGCTGGAGAGCTCACC
  AGGGAGGAGCTGAGGCAAATTGCAGAGGAGGATTTCTACGAAAAGCTGGCAGCTTCAATC
  GCCCCAGAAATATACGGGCATGAAGATGTGAAGAAGGCACTGCTGCTCCTGCTAGTCGGG
  GGTGTGGACCAGTCTCCTCGAGGCATGAAAATCCGGGGCAACATCAACATCTGTCTGATG
  GGGGATCCTGGTGTGGCCAAGTCTCAGCTCCTGTCATACATTGATCGACTGGCGCCTCGC
  AGCCAGTACACAACAGGCCGGGGCTCCTCAGGAGTGGGGCTTACGGCAGCTGTGCTGAGA
  GACTCCGTGAGTGGAGAACTGACCTTAGAGGGTGGGGCCCTGGTGCTGGCTGACCAGGGT
  GTGTGCTGCATTGATGAGTTCGACAAGATGGCTGAGGCCGACCGCACAGCCATCCACGAG
  GTCATGGAGCAGCAGACCATCTCCATTGCCAAGGCCGGCATTCTCACCACACTCAATGCC
  CGCTGCTCCATCCTGGCTGCCGCCAACCCTGCCTACGGGCGCTACAACCCTCGCCGCAGC
  CTGGAGCAGAACATACAGCTACCTGCTGCACTGCTCTCCCGGTTTGACCTCCTCTGGCTG
  ATTCAGGACCGGCCCGACCGAGACAATGACCTACGGTTGGCCCAGCACATTACCTATGTG
  CACCAGCACAGCCGGCAGCCCCCCTCCCAGTTTGAACCTCTGGACATGAAGCTCATGAGG
  CGTTACATAGCCATGTGCCGCGAGAAGCAGCCCATGGTGCCAGAGTCTCTGGCTGACTAC
  ATCACAGCAGCATACGTGGAGATGAGGCGAGAGGCTTGGGCTAGTAAGGATGCCACCTAT
  ACTTCTGCCCGGACCCTGCTGGCTATCCTGCGCCTTTCCACTGCTCTGGCACGTCTGAGA
  ATGGTGGATGTGGTGGAGAAAGAAGATGTGAATGAAGCCATCAGGCTAATGGAGATGTCA
  AAGGACTCTCTTCTAGGAGACAAGGGGCAGACAGCTAGGACTCAGAGACCAGCAGATGTG
  ATATTTGCCACCGTCCGTGAACTGGTCTCAGGGGGCCGAAGTGTCCGGTTCTCTGAGGCA
  GAGCAGCGCTGTGTATCTCGTGGCTTCACACCCGCCCAGTTCCAGGCGGCTCTGGATGAA
  TATGAGGAGCTCAATGTCTGGCAGGTCAATGCTTCCCGGACACGGATCACTTTTGTCTGA
  
  Clone variation with respect to NM_005916.3
  1611 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005916 unedited
  TCAGTATATTTGTATACGACTCACTATAGGGCGGCCCGCGAATTCGCACGAGGTGCGCTG
  GCCCTTTAAGAGCGATTCTCCTCCGCCCGCGCCAGCTCGGACCGCGGGAAACCCGGCGCC
  TGCACTACCCCGCCCGGAGATTCCCTTCCGACGCCCGCACCGCCTCCCCGTCACTCATTC
  TAGGCCCGCACGGTGATTGGCTTGCGGCTAGCGGGAGGTGAAGAAGGCCGCCTTGTCCGA
  TTGGCCCGCACGCAGTGGCGCCGGTCACGTGGGGGGCGACGTTTCGCGCCAATTTCGGTT
  GGCCGGCCACAGTCCACCGCGCGGAGATTCTCAGCTTCCCCAGGAGCAAGACCTCTGAGC
  CCGCCAAGCGCGGCCGCACGGCCCTCGGCAGCGATGGCACTGAAGGACTACGCGCTAGAG
  AAGGAAAAGGTTAAGAAGTTCTTACAAGAGTTCTACCAGGATGATGAACTCGGGAAGAAG
  CAGTTCAAGTATGGGAACCAGTTGGTTCGGCTGGCTCATCGGGAACAGGTGGCTCTGTAT
  GTGGACCTGGACGACGTANCCCGAGATGACCCCGAGTTGGTGGACTCAATTTGTGAGAAT
  GCCAGGCGCTACGCGAAGCTCTTTGCTGATGCCGTACAAGAGCTGCTGCCTCAGTCCAGG
  AGAGGGAAGTGTAAATAAAGATGCCTGGACGTTACATTGAGCATCGGCTATGATGGACAN
  NCGAGTCCAACCCTGGGATGGTCCGAGCCCCCAAACCAGTACCCTGCTGAACTCTGCGCA
  GATTGAGCTGTATTTTCAAGGCCTAACAGCACAGCCTCGTGTGATCCGGGAGTGCGGCTG
  ACTTGGGGGGAAGTGGTACTGTGCGTGAATCGNACTCGTGCTCTGAGTCAACCCAGATGG
  GGTGGCACCTAACTGGGACCGTGTGGGCAAAACTACAGCGATCAGNTCCCTTTATGCTTT
  GAATGGGCAGNCAGATGCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005916
• Insert Size: 2650 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005916.3, NP_005907.3
• RefSeq Size: 2821 bp
• RefSeq ORF: 2160 bp
• Locus ID: 4176
• Cytogenetics: 7q22.1
• Domains: MCM, AAA
• Protein Families: Transcription Factors
• Protein Pathways: Cell cycle, DNA replication
• Gene Summary: 'The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, and may regulate the binding of this protein with the tumorsuppressor protein RB1/RB. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) encodes the longer isoform (1).