PI4KA (NM_002650) Human Untagged Clone

CAT#: SC128296

PI4KA (untagged)-Human phosphatidylinositol 4-kinase, catalytic, alpha (PI4KA), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PI4KA
• Synonyms: PI4K-ALPHA; pi4K230; PIK4CA
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_002650 edited
  ATGCGGGAGATGGCAGGGGCCTGGCACATGACGGTGGAGCAGAAATTTGGCCTGTTTTCT
  GCTGAGATAAAGGAAGCAGACCCCCTGGCTGCCTCGGAAGCAAGTCAACCCAAACCCTGT
  CCCCCCGAAGTGACCCCCCACTACATCTGGATCGACTTCCTGGTGCAGCGGTTTGAGATC
  GCCAAGTACTGCAGCTCTGACCAAGTGGAGATCTTCTCCAGCCTGCTGCAGCGCTCCATG
  TCCCTGAACATCGGCGGGGCCAAGGGGAGCATGAACCGGCACGTGGCGGCCATCGGGCCC
  CGCTTCAAGCTGCTGACCCTGGGGCTGTCCCTCCTGCATGCCGATGTGGTTCCAAATGCA
  ACCATCCGCAATGTGCTTCGCGAGAAGATCTACTCCACTGCCTTTGACTACTTCAGCTGT
  CCCCCAAAGTTCCCTACTCAAGGAGAGAAGCGGCTGCGTGAAGACATAAGCATCATGATT
  AAATTTTGGACCGCCATGTTCTCAGATAAGAAGTACCTGACCGCCAGCCAGCTTGTTCCC
  CCAGATAATCAGGACACCCGGAGCAACCTGGACATAACTGTCGGCTCTCGGCAACAAGCC
  ACCCAAGGCTGGATCAACACATACCCCCTGTCCAGCGGCATGTCCACCATCTCCAAGAAA
  TCAGGCATGTCTAAGAAAACCAACCGGGGCTCCCAGCTGCACAAATACTACATGAAGCGC
  AGGACGCTGCTGCTGTCCCTGCTGGCCACTGAGATCGAGCGTCTCATCACATGGTACAAC
  CCGCTGTCAGCCCCGGAACTGGAACTAGACCAGGCCGGAGAGAACAGCGTGGCCAACTGG
  AGATCTAAGTACATCAGCCTGAGTGAGAAGCAGTGGAAGGACAACGTGAACCTCGCCTGG
  AGCATCTCTCCCTACCTAGCCGTGCAGCTGCCTGCCAGGTTTAAGAACACAGAAGCCATT
  GGGAACGAAGTGACCCGTCTCGTTCGGTTGGACCCGGGAGCCGTTAGTGATGTGCCTGAA
  GCAATCAAGTTCCTGGTCACCTGGCACACCATCGACGCCGATGCTCCAGAGCTCAGCCAT
  GTGCTGTGCTGGGCGCCCACGGACCCACCCACAGGCCTCTCCTACTTCTCCAGCATGTAC
  CCGCCGCACCCTCTCACGGCGCAGTACGGGGTGAAAGTCCTGCGGTCCTTCCCTCCGGAC
  GCCATCCTCTTCTACATCCCCCAGATTGTGCAGGCCCTCAGGTACGACAAGATGGGCTAT
  GTGCGGGAGTATATTCTGTGGGCAGCGTCTAAATCCCAGCTTCTGGCACACCAGTTCATC
  TGGAACATGAAGACTAACATTTATCTAGATGAAGAGGGCCACCAGAAAGACCCTGACATC
  GGCGACCTCCTGGATCAGTTGGTAGAGGAGATCACAGGCTCCTTGTCCGGCCCAGCGAAG
  GACTTTTACCAGCGGGAGTTTGATTTCTTTAACAAGATCACCAACGTGTCGGCTATCATC
  AAGCCCTACCCTAAAGGCGACGAGAGAAAGAAGGCTTGTCTGTCGGCCCTGTCTGAAGTG
  AAGGTGCAGCCGGGCTGCTACCTGCCCAGCAACCCTGAGGCCATTGTGCTGGACATCGAC
  TACAAGTCTGGGACCCCGATGCAGAGTGCTGCAAAAGCCCCATATCTGGCCAAGTTCAAG
  GTGAAGCGATGTGGAGTTAGTGAACTTGAAAAAGAAGGTCTGCGGTGCCGCTCAGACTCC
  GAGGATGAGTGCAGCACGCAGGAGGCCGACGGCCAGAAGATCTCCTGGCAGGCAGCCATC
  TTCAAGGTGGGAGACGACTGCCGGCAGGACATGCTGGCCCTGCAGATCATCGACCTCTTC
  AAGAACATCTTCCAGCTGGTCGGCCTGGACCTCTTTGTTTTTCCCTACCGCGTGGTGGCC
  ACTGCCCCTGGGTGCGGGGTGATCGAGTGCATCCCCGACTGCACCTCCCGGGACCAGCTG
  GGCCGCCAGACAGACTTCGGCATGTACGACTACTTCACACGCCAGTACGGGGATGAGTCC
  ACTCTGGCCTTCCAGCAGGCCCGCTACAACTTCATCCGAAGCATGGCCGCCTACAGCCTC
  CTGCTGTTCCTGCTGCAGATCAAGGACAGACACAACGGCAACATTATGCTGGACAAGAAG
  GGTCATATCATCCACATCGACTTTGGCTTCATGTTTGAAAGCTCGCCGGGCGGCAATCTC
  GGCTGGGAACCCGACATCAAGCTGACGGATGAGATGGTGATGATCATGGGGGGCAAGATG
  GAGGCCACACCCTTCAAGTGGTTCATGGAGATGTGTGTCCGAGGCTACCTGGCTGTGCGG
  CCCTACATGGACGCGGTCGTCTCCCTGGTCACTCTCATGTTGGACACGGGCCTGCCCTGT
  TTTCGCGGCCAGACAATCAAGCTCTTGAAGCACAGGTTTAGCCCCAACATGACTGAGCGC
  GAGGCTGCAAATTTCATCATGAAGGTCATCCAGAGCTGCTTCCTCAGCAACAGGAGCCGG
  ACCTACGACATGATCCAGTACTATCAGAATGACATCCCCTACTGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002650 unedited
  TACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGGGAGTGGCTGCTGGCTGGCAAGN
  ATGGAGTGGAAGTGCCGTTCATGCGGGAGATGGCAGGGGCCTGGCACATGACGGTGGAGC
  AGAAATTTGGCCTGTTTTCTGCTGAGATAAAGGAAGCAGACCCCCTGGCTGCCTCGGAAG
  CAAGTCAACCCAAACCCTGTCCCCCCGAAGTGACCCCCCACTACATCTGGATCGACTTCC
  TGGTGCAGCGGTTTGAGATCGCCAAGTACTGCAGCTCTGACCAAGTGGAGATCTTCTCCA
  GCCTGCTGCAGCGCTCCATGTCCCTGAACATCGGCGGGGCCAAGGGGAGCATGAACCGGC
  ACGTGGCGGCCATCGGGCCCCGCTTCAAGCTGCTGACCCTGGGGCTGTCCCTCCTGCATG
  CCGATGTGGTTCCAAATGCAACCATCCGCAATGTGCTTCGCGAGAAGATCTACTCCACTG
  CCTTTGACTACTTCAGCTGTCCCCCAAAGTTCCCTACTCAAGGAGAGAAGCGGCTGCGTG
  AAGACATAAGCATCATGATTAAATTTTGGACCGCCATGTTCTCAATTAAGAAGTACCTGA
  CCGCCAGCCAGCTTGTTCCCCCAGATAATCAGGACACCCGGAGCAACCTGACCATAACTG
  TCGGCTCTCGCAACAAGCCACCCAAGCCTGGATCACACATACCCCCTGTCCAGCGGCATG
  TCCACCATCTCCAGAAATCCAGCATGTCTAAAAAACCAACCGGGGGCTCCAGCTGCCANA
  TACTACATGAAGCGCAGGAGCTTNTTGCTGCCCTGCTGCCACTGAAAATCGACGTTAATA
  CTGGTCAAACCCCTGTAGCCCCGGACTGGAATAAACAGCCGGAAAAAAAGGTGGCACCTG
  GAAAATAAGACATACCCTGGGAAAGCAGGGGAAGCCACGGGACCTTCTGGAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002650 unedited
  CGTCGGATGCACTTCAGGGCCGGAGAGGCACTGGGGAGGGGTCACAGGGATGCCACCCGG
  GATCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTT
  TTTTTTTTCACTATTTTGGGTTTTTATTTTGTTGATGTTGGTTAAATCTTATCTCTTTTT
  TTATACACAATACTTCATGTACCTATGAAATAAGACAGGTAGGGAATATGTCCAGTGCAA
  ACAGAGGACTCACACCTGTGCATAGACAGCACCATCCATTGATTGTCGCTGCAGTCCATG
  GCGTTACCAAGGCTGCGCCACCCACGTGCTGCCCCAGGAGGCGCTACCAGGTTCTTTGGG
  CCACAGGCCTCTCCTCCACTGCATGTGGCGGCAGGGCAGGGAGGTCGCAGGGCTCCATGA
  TTGTGGGACAGCTTTGAGGGCACATGGGGCAAAGGCCCTCGAAGGTCCCCTCCTCAGTAG
  GGGATGTCATTCTGATAGTACTGGATCATGTCGTAGGTCCGGCTCCTGTTGCTGAGGAAG
  CAGCTCTGGATGACCTTCATGATGAAATTTGCAGCCTCGCGCTCAGTCATGTTGGGGCTA
  AACCTGTGCTTCAAGAGCTTGATTGTCTGGCCGCGAAAACAGGGCAGGCCCGTGTCCAAC
  ATGAGAGTGACCAGGGAGACGACCGCGTCCATGTAGGGCCGCACAGCCAGGTAGCCTCGG
  ACACACATCTCCATGAACCACTTGAAGGTGTGGCCTCCATCTTGCCCCCCATGATCATCA
  CCATCTCATCCGTCAGCTTGATGTCGGGTTCCCAGCCCGAGATTGCCGCCCGGCGAGCTT
  TCAACATGAGCAAGTCGATGTGATGATTGACCTCTGTCAGCATATGTGCGTTGTGTCTGT
  CTGATTGCACAGGACGCAGAGCCTGTAGCGCATGCCTCGGATGATTGAACGGCTGCTGAG
  CGAGTGGACTA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002650
• Insert Size: 3000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002650.1, NP_002641.1
• RefSeq Size: 3034 bp
• RefSeq ORF: 2565 bp
• Locus ID: 5297
• Cytogenetics: 22q11.21
• Domains: PI3_PI4_kinase, PI3Ka
• Protein Families: Druggable Genome
• Protein Pathways: Inositol phosphate metabolism, Metabolic pathways, Phosphatidylinositol signaling system
• Gene Summary: 'This gene encodes a phosphatidylinositol (PI) 4-kinase which catalyzes the first committed step in the biosynthesis of phosphatidylinositol 4,5-bisphosphate. The mammalian PI 4-kinases have been classified into two types, II and III, based on their molecular mass, and modulation by detergent and adenosine. The protein encoded by this gene is a type III enzyme that is not inhibited by adenosine. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Apr 2018]'
  Transcript Variant: This variant (2) differs in the 5' UTR and CDS compared to variant 1. The resulting isoform (2) is shorter at the N-terminus compared to isoform 1.