CACNA1A (NM_000068) Human Untagged Clone

CAT#: SC300004

CACNA1A (untagged)-Human calcium channel, voltage-dependent, P/Q type, alpha 1A subunit (CACNA1A), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CACNA1A
• Synonyms: APCA; BI; CACNL1A4; CAV2.1; EA2; EIEE42; FHM; HPCA; MHP; MHP1; SCA6
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_000068 edited
  ATGGCCCGCTTCGGAGACGAGATGCCGGCCCGCTACGGGGGAGGAGGCTCCGGGGCAGCC
  GCCGGGGTGGTCGTGGGCAGCGGAGGCGGGCGAGGAGCCGGGGGCAGCCGGCAGGGCGGG
  CAGCCCGGGGCGCAAAGGATGTACAAGCAGTCAATGGCGCAGAGAGCGCGGACCATGGCA
  CTCTACAACCCCATCCCCGTCCGACAGAACTGCCTCACGGTTAACCGGTCTCTCTTCCTC
  TTCAGCGAAGACAACGTGGTGAGAAAATACGCCAAAAAGATCACCGAATGGCCTCCCTTT
  GAATATATGATTTTAGCCACCATCATAGCGAATTGCATCGTCCTCGCACTGGAGCAGCAT
  CTGCCTGATGATGACAAGACCCCGATGTCTGAACGGCTGGATGACACAGAACCATACTTC
  ATTGGAATTTTTTGTTTCGAGGCTGGAATTAAAATCATTGCCCTTGGGTTTGCCTTCCAC
  AAAGGCTCCTACTTGAGGAATGGCTGGAATGTCATGGACTTTGTGGTGGTGCTAACGGGC
  ATCTTGGCGACAGTTGGGACGGAGTTTGACCTACGGACGCTGAGGGCAGTTCGAGTGCTG
  CGGCCGCTCAAGCTGGTGTCTGGAATCCCAAGTTTACAAGTCGTCCTGAAGTCGATCATG
  AAGGCGATGATCCCTTTGCTGCAGATCGGCCTCCTCCTATTTTTTGCAATCCTTATTTTT
  GCAATCATAGGGTTAGAATTTTATATGGGAAAATTTCATACCACCTGCTTTGAAGAGGGG
  ACAGATGACATTCAGGGTGAGTCTCCGGCTCCATGTGGGACAGAAGAGCCCGCCCGCACC
  TGCCCCAATGGGACCAAATGTCAGCCCTACTGGGAAGGGCCCAACAACGGGATCACTCAG
  TTCGACAACATCCTGTTTGCAGTGCTGACTGTTTTCCAGTGCATAACCATGGAAGGGTGG
  ACTGATCTCCTCTACAATAGCAACGATGCCTCAGGGAACACTTGGAACTGGTTGTACTTC
  ATCCCCCTCATCATCATCGGCTCCTTTTTTATGCTGAACCTTGTGCTGGGTGTGCTGTCA
  GGGGAGTTTGCCAAAGAAAGGGAACGGGTGGAGAACCGGCGGGCTTTTCTGAAGCTGAGG
  CGGCAACAACAGATTGAACGTGAGCTCAATGGGTACATGGAATGGATCTCAAAAGCAGAA
  GAGGTGATCCTCGCCGAGGATGAAACTGACGGGGAGCAGAGGCATCCCTTTGATGCTCTG
  CGGAGAACCACCATAAAGAAAAGCAAGACAGATTTGCTCAACCCCGAAGAGGCTGAGGAT
  CAGCTGGCTGATATAGCCTCTGTGGGTTCTCCCTTCGCCCGAGCCAGCATTAAAAGTGCC
  AAGCTGGAGAACTCGACCTTTTTTCACAAAAAGGAGAGGAGGATGCGTTTCTACATCCGC
  CGCATGGTCAAAACTCAGGCCTTCTACTGGACTGTACTCAGTTTGGTAGCTCTCAACACG
  CTGTGTGTTGCTATTGTTCACTACAACCAGCCCGAGTGGCTCTCCGACTTCCTTTACTAT
  GCAGAATTCATTTTCTTAGGACTCTTTATGTCCGAAATGTTTATAAAAATGTACGGGCTT
  GGGACGCGGCCTTACTTCCACTCTTCCTTCAACTGCTTTGACTGTGGGGTTATCATTGGG
  AGCATCTTCGAGGTCATCTGGGCTGTCATAAAACCTGGCACATCCTTTGGAATCAGCGTG
  TTACGAGCCCTCAGGTTATTGCGTATTTTCAAAGTCACAAAGTACTGGGCATCTCTCAGA
  AACCTGGTCGTCTCTCTCCTCAACTCCATGAAGTCCATCATCAGCCTGTTGTTTCTCCTT
  TTCCTGTTCATTGTCGTCTTCGCCCTTTTGGGAATGCAACTCTTCGGCGGCCAGTTTAAT
  TTCGATGAAGGGACTCCTCCCACCAACTTCGATACTTTTCCAGCAGCAATAATGACGGTG
  TTTCAGATCCTGACGGGCGAAGACTGGAACGAGGTCATGTACGACGGGATCAAGTCTCAG
  GGGGGCGTGCAGGGCGGCATGGTGTTCTCCATCTATTTCATTGTACTGACGCTCTTTGGG
  AACTACACCCTCCTGAATGTGTTCTTGGCCATCGCTGTGGACAATCTGGCCAACGCCCAG
  GAGCTCACCAAGGACGAGCAAGAGGAAGAAGAAGCAGCGAACCAGAAACTTGCCCTACAG
  AAAGCCAAGGAGGTGGCAGAAGTGAGTCCTCTGTCCGCGGCCAACATGTCTATAGCTGTG
  AAAGAGCAACAGAAGAATCAAAAGCCAGCCAAGTCCGTGTGGGAGCAGCGGACCAGTGAG
  ATGCGAAAGCAGAACTTGCTGGCCAGCCGGGAGGCCCTGTATAACGAAATGGACCCGGAC
  GAGCGCTGGAAGGCTGCCTACACGCGGCACCTGCGGCCAGACATGAAGACGCACTTGGAC
  CGGCCGCTGGTGGTGGACCCGCAGGAGAACCGCAACAACAACACCAACAAGAGCCGGGCG
  GCCGAGCCCACCGTGGACCAGCGCCTCGGCCAGCAGCGCGCCGAGGACTTCCTCAGGAAA
  CAGGCCCGCTACCACGATCGGGCCCGGGACCCCAGCGGCTCGGCGGGCCTGGACGCACGG
  AGGCCCTGGGCGGGAAGCCAGGAGGCCGAGCTGAGCCGGGAGGGACCCTACGGCCGCGAG
  TCGGACCACCACGCCCGGGAGGGCAGCCTGGAGCAACCCGGGTTCTGGGAGGGCGAGGCC
  GAGCGAGGCAAGGCCGGGGACCCCCACCGGAGGCACGTGCACCGGCAGGGGGGCAGCAGG
  GAGAGCCGCAGCGGGTCCCCGCGCACGGGCGCGGACGGGGAGCATCGACGTCATCGCGCG
  CACCGCAGGCCCGGGGAGGAGGGTCCGGAGGACAAGGCGGAGCGGAGGGCGCGGCACCGC
  GAGGGCAGCCGGCCGGCCCGGGGCGGCGAGGGCGAGGGCGAGGGCCCCGACGGGGGCGAG
  CGCAGGAGAAGGCACCGGCATGGCGCTCCAGCCACGTACGAGGGGGACGCGCGGAGGGAG
  GACAAGGAGCGGAGGCATCGGAGGAGGAAAGAGAACCAGGGCTCCGGGGTCCCTGTGTCG
  GGCCCCAACCTGTCAACCACCCGGCCAATCCAGCAGGACCTGGGCCGCCAAGACCCACCC
  CTGGCAGAGGATATTGACAACATGAAGAACAACAAGCTGGCCACCGCGGAGTCGGCCGCT
  CCCCACGGCAGCCTTGGCCACGCCGGCCTGCCCCAGAGCCCAGCCAAGATGGGAAACAGC
  ACCGACCCCGGCCCCATGCTGGCCATCCCTGCCATGGCCACCAACCCCCAGAACGCCGCC
  AGCCGCCGGACGCCCAACAACCCGGGGAACCCATCCAATCCCGGCCCCCCCAAGACCCCC
  GAGAATAGCCTTATCGTCACCAACCCCAGCGGCACCCAGACCAATTCAGCTAAGACTGCC
  AGGAAACCCGACCACACCACAGTGGACATCCCCCCAGCCTGCCCACCCCCCCTCAACCAC
  ACCGTCGTACAAGTGAACAAAAACGCCAACCCAGACCCACTGCCAAAAAAAGAGGAAGAG
  AAGAAGGAGGAGGAGGAAGACGACCGTGGGGAAGACGGCCCTAAGCCAATGCCTCCCTAT
  AGCTCCATGTTCATCCTGTCCACGACCAACCCCCTTCGCCGCCTGTGCCATTACATCCTG
  AACCTGCGCTACTTTGAGATGTGCATCCTCATGGTCATTGCCATGAGCAGCATCGCCCTG
  GCCGCCGAGGACCCTGTGCAGCCCAACGCACCTCGGAACAACGTGCTGCGATACTTTGAC
  TACGTTTTTACAGGCGTCTTTACCTTTGAGATGGTGATCAAGATGATTGACCTGGGGCTC
  GTCCTGCATCAGGGTGCCTACTTCCGTGACCTCTGGAATATTCTCGACTTCATAGTGGTC
  AGTGGGGCCCTGGTAGCCTTTGCCTTCACTGGCAATAGCAAAGGAAAAGACATCAACACG
  ATTAAATCCCTCCGAGTCCTCCGGGTGCTACGACCTCTTAAAACCATCAAGCGGCTGCCA
  AAGCTCAAGGCTGTGTTTGACTGTGTGGTGAACTCACTTAAAAACGTCTTCAACATCCTC
  ATCGTCTACATGCTATTCATGTTCATCTTCGCCGTGGTGGCTGTGCAGCTCTTCAAGGGG
  AAATTCTTCCACTGCACTGACGAGTCCAAAGAGTTTGAGAAAGATTGTCGAGGCAAATAC
  CTCCTCTACGAGAAGAATGAGGTGAAGGCGCGAGACCGGGAGTGGAAGAAGTATGAATTC
  CATTACGACAATGTGCTGTGGGCTCTGCTGACCCTCTTCACCGTGTCCACGGGAGAAGGC
  TGGCCACAGGTCCTCAAGCATTCGGTGGACGCCACCTTTGAGAACCAGGGCCCCAGCCCC
  GGGTACCGCATGGAGATGTCCATTTTCTACGTCGTCTACTTTGTGGTGTTCCCCTTCTTC
  TTTGTCAATATCTTTGTGGCCTTGATCATCATCACCTTCCAGGAGCAAGGGGACAAGATG
  ATGGAGGAATACAGCCTGGAGAAAAATGAGAGGGCCTGCATTGATTTCGCCATCAGCGCC
  AAGCCGCTGACCCGACACATGCCGCAGAACAAGCAGAGCTTCCAGTACCGCATGTGGCAG
  TTCGTGGTGTCTCCGCCTTTCGAGTACACGATCATGGCCATGATCGCCCTCAACACCATC
  GTGCTTATGATGAAGTTCTATGGGGCTTCTGTTGCTTATGAAAATGCCCTGCGGGTGTTC
  AACATCGTCTTCACCTCCCTCTTCTCTCTGGAATGTGTGCTGAAAGTCATGGCTTTTGGG
  ATTCTGAATTATTTCCGCGATGCCTGGAACATCTTCGACTTTGTGACTGTTCTGGGCAGC
  ATCACCGATATCCTCGTGACTGAGTTTGGGAATAACTTCATCAACCTGAGCTTTCTCCGC
  CTCTTCCGAGCTGCCCGGCTCATCAAACTTCTCCGTCAGGGTTACACCATCCGCATTCTT
  CTCTGGACCTTTGTGCAGTCCTTCAAGGCCCTGCCTTATGTCTGTCTGCTGATCGCCATG
  CTCTTCTTCATCTATGCCATCATTGGGATGCAGGTGTTTGGTAACATTGGCATCGACGTG
  GAGGACGAGGACAGTGATGAAGATGAGTTCCAAATCACTGAGCACAATAACTTCCGGACC
  TTCTTCCAGGCCCTCATGCTTCTCTTCCGGAGTGCCACCGGGGAAGCTTGGCACAACATC
  ATGCTTTCCTGCCTCAGCGGGAAACCGTGTGATAAGAACTCTGGCATCCTGACTCGAGAG
  TGTGGCAATGAATTTGCTTATTTTTACTTTGTTTCCTTCATCTTCCTCTGCTCGTTTCTG
  ATGCTGAATCTCTTTGTCGCCGTCATCATGGACAACTTTGAGTACCTCACCCGAGACTCC
  TCCATCCTGGGCCCCCACCACCTGGATGAGTACGTGCGTGTCTGGGCCGAGTATGACCCC
  GCAGCTTGCGGTCGGATTCATTATAAGGATATGTACAGTTTATTACGAGTAATATCTCCC
  CCTCTCGGCTTAGGCAAGAAATGTCCTCATAGGGTTGCTTGCAAGCGGCTTCTGCGGATG
  GACCTGCCCGTCGCAGATGACAACACCGTCCACTTCAATTCCACCCTCATGGCTCTGATC
  CGCACAGCCCTGGACATCAAGATTGCCAAGGGAGGAGCCGACAAACAGCAGATGGACGCT
  GAGCTGCGGAAGGAGATGATGGCGATTTGGCCCAATCTGTCCCAGAAGACGCTAGACCTG
  CTGGTCACACCTCACAAGTCCACGGACCTCACCGTGGGGAAGATCTACGCAGCCATGATG
  ATCATGGAGTACTACCGGCAGAGCAAGGCCAAGAAGCTGCAGGCCATGCGCGAGGAGCAG
  GACCGGACACCCCTCATGTTCCAGCGCATGGAGCCCCCGTCCCCAACGCAGGAAGGGGGA
  CCTGGCCAGAACGCCCTCCCCTCCACCCAGCTGGACCCAGGAGGAGCCCTGATGGCTCAC
  GAAAGCGGCCTCAAGGAGAGCCCGTCCTGGGTGACCCAGCGTGCCCAGGAGATGTTCCAG
  AAGACGGGCACATGGAGTCCGGAACAAGGCCCCCCTACCGACATGCCCAACAGCCAGCCT
  AACTCTCAGTCCGTGGAGATGCGAGAGATGGGCAGAGATGGCTACTCCGACAGCGAGCAC
  TACCTCCCCATGGAAGGCCAGGGCCGGGCTGCCTCCATGCCCCGCCTCCCTGCAGAGAAC
  CAGAGGAGAAGGGGCCGGCCACGTGGGAATAACCTCAGTACCATCTCAGACACCAGCCCC
  ATGAAGCGTTCAGCCTCCGTGCTGGGCCCCAAGGCCCGACGCCTGGACGATTACTCGCTG
  GAGCGGGTCCCGCCCGAGGAGAACCAGCGGCACCACCAGCGGCGCCGCGACCGCAGCCAC
  CGCGCCTCTGAGCGCTCCCTGGGCCGCTACACCGATGTGGACACAGGCTTGGGGACAGAC
  CTGAGCATGACCACCCAATCCGGGGACCTGCCGTCGAAGGAGCGGGACCAGGAGCGGGGC
  CGGCCCAAGGATCGGAAGCATCGACAGCACCACCACCACCACCACCACCACCACCACCCC
  CCGCCCCCCGACAAGGACCGCTATGCCCAGGAACGGCCGGACCACGGCCGGGCACGGGCT
  CGGGACCAGCGCTGGTCCCGCTCGCCCAGCGAGGGCCGAGAGCACATGGCGCACCGGCAG
  TAG
  
• Restriction Sites: Please inquire
• ACCN: NM_000068
• Insert Size: 7100 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: The open reading frame of this clone has been fully sequenced and found a three base deletion and one SNP within the protein associated with this reference, NM_000068.2. This SNP doesn't change amino acid.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000068.2, NP_000059.2
• RefSeq Size: 7807 bp
• RefSeq ORF: 6786 bp
• Locus ID: 773
• Cytogenetics: 19p13.13
• Protein Families: Druggable Genome, Ion Channels: Calcium, Transmembrane
• Protein Pathways: Calcium signaling pathway, Long-term depression, MAPK signaling pathway, Taste transduction, Type II diabetes mellitus
• Gene Summary: 'Voltage-dependent calcium channels mediate the entry of calcium ions into excitable cells, and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, and gene expression. Calcium channels are multisubunit complexes composed of alpha-1, beta, alpha-2/delta, and gamma subunits. The channel activity is directed by the pore-forming alpha-1 subunit, whereas, the others act as auxiliary subunits regulating this activity. The distinctive properties of the calcium channel types are related primarily to the expression of a variety of alpha-1 isoforms, alpha-1A, B, C, D, E, and S. This gene encodes the alpha-1A subunit, which is predominantly expressed in neuronal tissue. Mutations in this gene are associated with 2 neurologic disorders, familial hemiplegic migraine and episodic ataxia 2. This gene also exhibits polymorphic variation due to (CAG)n-repeats. Multiple transcript variants encoding different isoforms have been found for this gene. In one set of transcript variants, the (CAG)n-repeats occur in the 3' UTR, and are not associated with any disease. But in another set of variants, an insertion extends the coding region to include the (CAG)n-repeats which encode a polyglutamine tract. Expansion of the (CAG)n-repeats from the normal 4-18 to 21-33 in the coding region is associated with spinocerebellar ataxia 6. [provided by RefSeq, Jul 2016]'
  Transcript Variant: This variant (1) uses an alternate splice site in the 3' coding region that results in a frameshift, compared to variant 2. The resulting isoform (1) has a shorter and distinct C-terminus that does not include the polyglutamine tract, compared to isoform 2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no quality transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.