Collagen V (COL5A1) (NM_000093) Human Untagged Clone

CAT#: SC300010

COL5A1 (untagged)-Human collagen, type V, alpha 1 (COL5A1)


  "NM_000093" in other vectors (2)

Reconstitution Protocol

USD 3,730.00

8 Weeks*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol COL5A1
Synonyms EDSC; EDSCL1
Vector pCMV6 series
Sequence Data
>NCBI ORF sequence for NM_000093, the custom clone sequence may differ by one or more nucleotides
ATGGACGTCCATACCCGCTGGAAAGCGCGCAGCGCGCTCCGCCCGGGCGCCCCGCTGCTG
CCCCCGCTGCTGCTGCTGCTGCTGTGGGCGCCGCCTCCGAGCCGCGCAGCTCAGCCAGCA
GATCTCCTGAAGGTTCTAGATTTTCACAACTTGCCTGATGGAATAACAAAGACAACAGGC
TTTTGCGCCACGCGGCGATCTTCCAAAGGCCCGGATGTCGCTTACAGAGTCACCAAAGAC
GCGCAGCTCAGCGCACCCACCAAGCAGCTGTACCCTGCGTCTGCATTTCCCGAGGACTTC
TCCATCCTAACAACTGTGAAAGCCAAGAAAGGCAGCCAGGCCTTCCTGGTCTCCATCTAC
AACGAGCAGGGTATCCAGCAGATTGGGCTGGAGCTGGGCCGCTCTCCCGTCTTCCTCTAC
GAGGACCACACGGGGAAGCCTGGCCCGGAAGACTACCCCCTCTTCCGGGGCATCAACCTG
TCAGATGGCAAGTGGCACAGAATTGCTCTCAGCGTCCACAAGAAAAATGTCACCTTGATC
CTCGACTGTAAAAAGAAGACCACCAAATTCCTCGACCGCAGCGACCACCCCATGATCGAC
ATCAATGGCATCATCGTGTTTGGCACCCGGATCCTGGATGAGGAGGTGTTTGAGGGTGAC
ATCCAGCAGCTGCTCTTTGTCTCGGACCACCGGGCAGCTTATGATTACTGTGAGCACTAC
AGCCCTGACTGTGACACCGCAGTACCTGACACCCCACAGTCGCAGGACCCCAATCCAGAT
GAATATTACACGGAAGGAGACGGCGAGGGTGAGACCTATTACTACGAATACCCCTACTAC
GAAGACCCCGAAGACCTAGGGAAGGAGCCCACCCCCAGCAAGAAGCCCGTGGAAGCTGCC
AAAGAAACCACAGAGGTCCCCGAGGAGCTGACCCCGACCCCCACGGAAGCTGCTCCCATG
CCTGAAACCAGTGAAGGGGCTGGGAAGGAAGAGGACGTCGGCATCGGGGACTATGACTAC
GTGCCCAGTGAGGACTACTACACGCCCTCACCGTATGATGACCTCACCTATGGCGAGGGG
GAGGAGAACCCCGACCAGCCCACAGACCCAGGCGCTGGGGCCGAAATTCCCACCAGCACC
GCCGACACCTCCAACTCCTCCAATCCAGCTCCGCCTCCAGGGGAAGGTGCGGATGACTTG
GAGGGGGAGTTCACTGAGGAAACGATCCGGAACCTTGACGAGAACTACTACGACCCCTAC
TACGACCCCACCAGCTCCCCGTCGGAGATCGGGCCGGGAATGCCGGCGAACCAGGATACC
ATCTATGAAGGGATTGGAGGACCTCGGGGCGAGAAAGGCCAAAAGGGAGAACCAGCGATT
ATCGAGCCGGGCATGCTCATCGAGGGCCCGCCTGGCCCAGAAGGCCCCGCGGGTCTTCCC
GGACCTCCAGGAACCATGGGTCCCACTGGCCAAGTCGGGGACCCTGGAGAAAGGGGCCCC
CCTGGACGCCCAGGCCTTCCTGGGGCCGATGGCCTGCCCGGTCCTCCAGGAACCATGCTC
ATGCTGCCCTTCCGGTTTGGAGGTGGCGGCGATGCGGGCTCCAAAGGCCCCATGGTCTCA
GCCCAGGAGTCCCAGGCGCAAGCCATTCTCCAGCAGGCCAGGTTGGCACTGAGGGGACCA
GCTGGCCCGATGGGTCTCACAGGGAGACCTGGCCCTGTGGGTCCCCCTGGGAGCGGAGGT
TTGAAGGGCGAGCCGGGAGACGTGGGGCCTCAGGGTCCTCGAGGTGTGCAAGGCCCGCCT
GGTCCGGCCGGGAAGCCCGGAAGACGGGGTCGGGCTGGGAGTGATGGAGCCAGAGGAATG
CCTGGACAAACTGGCCCCAAGGGTGACCGGGGTTTCGACGGCCTGGCTGGGTTGCCAGGC
GAGAAGGGCCACAGGGGTGACCCTGGTCCTTCCGGCCCACCAGGACCTCCGGGAGACGAT
GGAGAAAGGGGTGACGACGGAGAAGTTGGGCCCAGGGGGCTGCCTGGGGAGCCCGGGCCA
CGTGGTCTGCTTGGGCCGAAGGGGCCCCCAGGTCCTCCCGGACCTCCCGGTGTCACGGGT
ATGGACGGCCAGCCGGGGCCAAAAGGAAATGTGGGTCCCCAGGGAGAGCCTGGCCCCCCA
GGACAGCAGGGTAATCCAGGCGCCCAGGGTCTTCCAGGCCCCCAGGGTGCAATTGGTCCT
CCAGGAGAAAAGGGTCCCTTGGGGAAACCAGGCCTTCCAGGAATGCCCGGTGCTGACGGA
CCCCCGGGACACCCTGGCAAAGAAGGCCCTCCAGGAGAGAAAGGAGGTCAGGGTCCACCT
GGCCCCCAGGGTCCGATTGGCTACCCAGGTCCTCGAGGAGTCAAGGGGGCCGATGGCATC
CGTGGTCTGAAGGGCACAAAGGGCGAGAAGGGTGAAGACGGCTTTCCTGGGTTTAAAGGA
GACATGGGCATCAAGGGTGATCGGGGGGAGATCGGCCCACCCGGTCCCAGGGGAGAAGAT
GGCCCTGAAGGCCCAAAGGGTCGCGGAGGTCCCAATGGTGACCCCGGTCCTCTGGGACCC
CCTGGGGAGAAGGGAAAACTCGGAGTCCCAGGGTTACCAGGGTATCCAGGAAGACAAGGA
CCAAAGGGCTCTATTGGATTCCCTGGATTTCCTGGCGCCAATGGAGAGAAGGGCGGCAGG
GGGACCCCTGGAAAGCCAGGACCGCGGGGGCAGCGAGGCCCAACGGGTCCGAGGGGTGAA
AGAGGCCCCCGGGGCATCACTGGGAAGCCTGGCCCCAAGGGCAACTCCGGAGGTGACGGC
CCAGCTGGCCCTCCTGGTGAACGGGGACCCAATGGACCCCAAGGACCCACAGGATTTCCT
GGACCAAAGGGCCCCCCTGGCCCTCCAGGCAAGGATGGACTCCCAGGACACCCTGGACAG
AGAGGCGAGACTGGTTTCCAAGGCAAGACCGGCCCTCCAGGCCCCCCCGGCGTGGTCGGC
CCTCAGGGTCCCACGGGAGAAACGGGCCCAATGGGTGAGCGTGGCCACCCTGGGCCCCCT
GGACCCCCCGGTGAACAGGGGCTTCCGGGCCTTGCTGGAAAAGAAGGGACGAAGGGTGAC
CCAGGCCCTGCAGGCCTCCCTGGGAAAGATGGCCCTCCAGGATTACGTGGTTTCCCTGGG
GACCGAGGGCTTCCTGGTCCAGTGGGAGCTCTTGGACTGAAAGGCAATGAAGGGCCCCCT
GGCCCACCAGGCCCTGCGGGATCTCCAGGGGAGAGAGGTCCAGCTGGAGCCGCTGGGCCC
ATCGGAATTCCAGGGAGACCTGGGCCCCAGGGACCCCCAGGGCCGGCAGGAGAGAAAGGG
GCTCCTGGCGAGAAAGGCCCACAAGGCCCAGCTGGCCGAGACGGTCTCCAGGGGCCTGTG
GGGCTCCCGGGTCCAGCTGGCCCTGTGGGTCCCCCTGGAGAAGACGGAGATAAGGGAGAG
ATCGGGGAGCCGGGGCAGAAAGGAAGCAAGGGGGACAAAGGAGAACAGGGTCCTCCTGGG
CCTACAGGTCCTCAAGGCCCCATCGGACAGCCAGGCCCCTCTGGAGCTGACGGCGAGCCG
GGGCCTCGGGGCCAGCAGGGCCTTTTCGGGCAGAAAGGTGATGAAGGTCCCAGAGGCTTT
CCTGGACCCCCTGGGCCAGTGGGGCTGCAGGGTTTGCCAGGACCTCCAGGCGAGAAGGGT
GAGACAGGAGACGTGGGCCAGATGGGCCCCCCGGGTCCCCCTGGCCCCCGAGGACCCTCC
GGAGCTCCAGGTGCTGATGGCCCACAAGGTCCCCCAGGTGGAATAGGAAACCCTGGTGCA
GTGGGAGAGAAGGGCGAGCCTGGCGAAGCAGGTGAGCCTGGCCTTCCGGGAGAAGGCGGC
CCCCCGGGACCCAAAGGAGAAAGGGGAGAGAAGGGCGAGTCAGGCCCTTCAGGTGCTGCC
GGACCCCCTGGACCCAAAGGCCCTCCCGGAGATGATGGTCCCAAAGGCAGCCCTGGCCCA
GTGGGTTTTCCTGGAGATCCTGGCCCCCCCGGAGAGCCTGGCCCCGCGGGTCAAGATGGT
CCCCCTGGTGACAAAGGAGATGATGGTGAACCCGGGCAGACGGGATCCCCCGGCCCTACT
GGTGAACCAGGTCCATCGGGGCCTCCAGGAAAAAGGGGTCCCCCAGGCCCCGCAGGCCCC
GAAGGCAGACAGGGAGAGAAAGGGGCCAAGGGAGAAGCCGGCTTGGAAGGCCCTCCTGGG
AAGACTGGCCCCATCGGCCCCCAGGGGGCCCCTGGGAAGCCCGGACCGGATGGCCTTCGA
GGGATCCCTGGCCCTGTGGGAGAACAAGGTCTCCCAGGATCCCCAGGCCCGGACGGTCCC
CCCGGCCCCATGGGTCCCCCAGGACTTCCCGGCCTCAAAGGAGATTCTGGTCCCAAAGGT
GAAAAGGGTCATCCAGGCCTGATCGGGCTCATCGGTCCTCCGGGTGAACAGGGTGAGAAG
GGCGACCGTGGTCTCCCTGGCCCCCAGGGCTCCTCCGGTCCTAAGGGAGAACAGGGTATC
ACTGGTCCTTCTGGCCCGATTGGGCCTCCTGGGCCCCCTGGCCTGCCGGGTCCGCCTGGT
CCAAAAGGTGCTAAGGGCTCCTCGGGTCCAACTGGCCCGAAGGGTGAGGCAGGCCACCCA
GGACCCCCAGGCCCCCCGGGCCCCCCGGGAGAGGTCATCCAGCCCCTGCCAATCCAGGCA
TCCAGGACGCGGCGGAACATCGACGCCAGCCAGCTGCTGGACGACGGGAATGGCGAGAAC
TACGTGGACTACGCGGACGGCATGGAAGAGATCTTCGGCTCTCTCAACTCTCTGAAGCTG
GAGATTGAGCAGATGAAACGGCCCCTGGGCACGCAGCAGAACCCCGCCCGCACCTGCAAG
GACCTGCAGCTCTGCCACCCCGACTTCCCAGATGGTGAATACTGGGTCGATCCTAACCAA
GGATGCTCCAGGGATTCCTTCAAGGTTTACTGCAACTTCACAGCCGGGGGGTCGACATGC
GTCTTCCCTGACAAGAAGTCCGAAGGGGCCAGAATCACTTCTTGGCCCAAAGAAAACCCG
GGCTCCTGGTTCAGTGAATTCAAGCGTGGGAAACTGCTCTCCTATGTGGACGCCGAGGGC
AACCCTGTGGGTGTGGTACAGATGACCTTCCTGCGGCTGCTGAGCGCCTCTGCCCACCAG
AACGTCACCTACCACTGCTACCAGTCAGTGGCCTGGCAGGACGCAGCCACGGGCAGCTAC
GACAAGGCCCTCCGCTTCCTGGGCTCCAACGACGAGGAGATGTCCTATGACAACAACCCC
TACATCCGCGCCCTGGTGGACGGCTGTGCTACCAAGAAAGGCTACCAGAAGACGGTTCTG
GAGATCGACACCCCCAAAGTGGAGCAGGTGCCCATCGTGGACATCATGTTCAATGACTTC
GGTGAAGCGTCACAGAAATTTGGATTTGAAGTGGGGCCGGCTTGCTTCATGGGCTAG
Restriction Sites Please inquire     
ACCN NM_000093
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_000093.2, NP_000084.2
RefSeq Size 6496 bp
RefSeq ORF 5517 bp
Locus ID 1289
Cytogenetics 9q34.3
Protein Families Druggable Genome
Protein Pathways ECM-receptor interaction, Focal adhesion
Gene Summary 'This gene encodes an alpha chain for one of the low abundance fibrillar collagens. Fibrillar collagen molecules are trimers that can be composed of one or more types of alpha chains. Type V collagen is found in tissues containing type I collagen and appears to regulate the assembly of heterotypic fibers composed of both type I and type V collagen. This gene product is closely related to type XI collagen and it is possible that the collagen chains of types V and XI constitute a single collagen type with tissue-specific chain combinations. The encoded procollagen protein occurs commonly as the heterotrimer pro-alpha1(V)-pro-alpha1(V)-pro-alpha2(V). Mutations in this gene are associated with Ehlers-Danlos syndrome, types I and II. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, May 2013]'
Transcript Variant: This variant (1) encodes isoform 1.

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.