Collagen V (COL5A1) (NM_000093) Human Untagged Clone

CAT#: SC300010

COL5A1 (untagged)-Human collagen, type V, alpha 1 (COL5A1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: COL5A1
• Synonyms: EDSC; EDSCL1
• Vector: pCMV6 series
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_000093, the custom clone sequence may differ by one or more nucleotides
  ATGGACGTCCATACCCGCTGGAAAGCGCGCAGCGCGCTCCGCCCGGGCGCCCCGCTGCTG
  CCCCCGCTGCTGCTGCTGCTGCTGTGGGCGCCGCCTCCGAGCCGCGCAGCTCAGCCAGCA
  GATCTCCTGAAGGTTCTAGATTTTCACAACTTGCCTGATGGAATAACAAAGACAACAGGC
  TTTTGCGCCACGCGGCGATCTTCCAAAGGCCCGGATGTCGCTTACAGAGTCACCAAAGAC
  GCGCAGCTCAGCGCACCCACCAAGCAGCTGTACCCTGCGTCTGCATTTCCCGAGGACTTC
  TCCATCCTAACAACTGTGAAAGCCAAGAAAGGCAGCCAGGCCTTCCTGGTCTCCATCTAC
  AACGAGCAGGGTATCCAGCAGATTGGGCTGGAGCTGGGCCGCTCTCCCGTCTTCCTCTAC
  GAGGACCACACGGGGAAGCCTGGCCCGGAAGACTACCCCCTCTTCCGGGGCATCAACCTG
  TCAGATGGCAAGTGGCACAGAATTGCTCTCAGCGTCCACAAGAAAAATGTCACCTTGATC
  CTCGACTGTAAAAAGAAGACCACCAAATTCCTCGACCGCAGCGACCACCCCATGATCGAC
  ATCAATGGCATCATCGTGTTTGGCACCCGGATCCTGGATGAGGAGGTGTTTGAGGGTGAC
  ATCCAGCAGCTGCTCTTTGTCTCGGACCACCGGGCAGCTTATGATTACTGTGAGCACTAC
  AGCCCTGACTGTGACACCGCAGTACCTGACACCCCACAGTCGCAGGACCCCAATCCAGAT
  GAATATTACACGGAAGGAGACGGCGAGGGTGAGACCTATTACTACGAATACCCCTACTAC
  GAAGACCCCGAAGACCTAGGGAAGGAGCCCACCCCCAGCAAGAAGCCCGTGGAAGCTGCC
  AAAGAAACCACAGAGGTCCCCGAGGAGCTGACCCCGACCCCCACGGAAGCTGCTCCCATG
  CCTGAAACCAGTGAAGGGGCTGGGAAGGAAGAGGACGTCGGCATCGGGGACTATGACTAC
  GTGCCCAGTGAGGACTACTACACGCCCTCACCGTATGATGACCTCACCTATGGCGAGGGG
  GAGGAGAACCCCGACCAGCCCACAGACCCAGGCGCTGGGGCCGAAATTCCCACCAGCACC
  GCCGACACCTCCAACTCCTCCAATCCAGCTCCGCCTCCAGGGGAAGGTGCGGATGACTTG
  GAGGGGGAGTTCACTGAGGAAACGATCCGGAACCTTGACGAGAACTACTACGACCCCTAC
  TACGACCCCACCAGCTCCCCGTCGGAGATCGGGCCGGGAATGCCGGCGAACCAGGATACC
  ATCTATGAAGGGATTGGAGGACCTCGGGGCGAGAAAGGCCAAAAGGGAGAACCAGCGATT
  ATCGAGCCGGGCATGCTCATCGAGGGCCCGCCTGGCCCAGAAGGCCCCGCGGGTCTTCCC
  GGACCTCCAGGAACCATGGGTCCCACTGGCCAAGTCGGGGACCCTGGAGAAAGGGGCCCC
  CCTGGACGCCCAGGCCTTCCTGGGGCCGATGGCCTGCCCGGTCCTCCAGGAACCATGCTC
  ATGCTGCCCTTCCGGTTTGGAGGTGGCGGCGATGCGGGCTCCAAAGGCCCCATGGTCTCA
  GCCCAGGAGTCCCAGGCGCAAGCCATTCTCCAGCAGGCCAGGTTGGCACTGAGGGGACCA
  GCTGGCCCGATGGGTCTCACAGGGAGACCTGGCCCTGTGGGTCCCCCTGGGAGCGGAGGT
  TTGAAGGGCGAGCCGGGAGACGTGGGGCCTCAGGGTCCTCGAGGTGTGCAAGGCCCGCCT
  GGTCCGGCCGGGAAGCCCGGAAGACGGGGTCGGGCTGGGAGTGATGGAGCCAGAGGAATG
  CCTGGACAAACTGGCCCCAAGGGTGACCGGGGTTTCGACGGCCTGGCTGGGTTGCCAGGC
  GAGAAGGGCCACAGGGGTGACCCTGGTCCTTCCGGCCCACCAGGACCTCCGGGAGACGAT
  GGAGAAAGGGGTGACGACGGAGAAGTTGGGCCCAGGGGGCTGCCTGGGGAGCCCGGGCCA
  CGTGGTCTGCTTGGGCCGAAGGGGCCCCCAGGTCCTCCCGGACCTCCCGGTGTCACGGGT
  ATGGACGGCCAGCCGGGGCCAAAAGGAAATGTGGGTCCCCAGGGAGAGCCTGGCCCCCCA
  GGACAGCAGGGTAATCCAGGCGCCCAGGGTCTTCCAGGCCCCCAGGGTGCAATTGGTCCT
  CCAGGAGAAAAGGGTCCCTTGGGGAAACCAGGCCTTCCAGGAATGCCCGGTGCTGACGGA
  CCCCCGGGACACCCTGGCAAAGAAGGCCCTCCAGGAGAGAAAGGAGGTCAGGGTCCACCT
  GGCCCCCAGGGTCCGATTGGCTACCCAGGTCCTCGAGGAGTCAAGGGGGCCGATGGCATC
  CGTGGTCTGAAGGGCACAAAGGGCGAGAAGGGTGAAGACGGCTTTCCTGGGTTTAAAGGA
  GACATGGGCATCAAGGGTGATCGGGGGGAGATCGGCCCACCCGGTCCCAGGGGAGAAGAT
  GGCCCTGAAGGCCCAAAGGGTCGCGGAGGTCCCAATGGTGACCCCGGTCCTCTGGGACCC
  CCTGGGGAGAAGGGAAAACTCGGAGTCCCAGGGTTACCAGGGTATCCAGGAAGACAAGGA
  CCAAAGGGCTCTATTGGATTCCCTGGATTTCCTGGCGCCAATGGAGAGAAGGGCGGCAGG
  GGGACCCCTGGAAAGCCAGGACCGCGGGGGCAGCGAGGCCCAACGGGTCCGAGGGGTGAA
  AGAGGCCCCCGGGGCATCACTGGGAAGCCTGGCCCCAAGGGCAACTCCGGAGGTGACGGC
  CCAGCTGGCCCTCCTGGTGAACGGGGACCCAATGGACCCCAAGGACCCACAGGATTTCCT
  GGACCAAAGGGCCCCCCTGGCCCTCCAGGCAAGGATGGACTCCCAGGACACCCTGGACAG
  AGAGGCGAGACTGGTTTCCAAGGCAAGACCGGCCCTCCAGGCCCCCCCGGCGTGGTCGGC
  CCTCAGGGTCCCACGGGAGAAACGGGCCCAATGGGTGAGCGTGGCCACCCTGGGCCCCCT
  GGACCCCCCGGTGAACAGGGGCTTCCGGGCCTTGCTGGAAAAGAAGGGACGAAGGGTGAC
  CCAGGCCCTGCAGGCCTCCCTGGGAAAGATGGCCCTCCAGGATTACGTGGTTTCCCTGGG
  GACCGAGGGCTTCCTGGTCCAGTGGGAGCTCTTGGACTGAAAGGCAATGAAGGGCCCCCT
  GGCCCACCAGGCCCTGCGGGATCTCCAGGGGAGAGAGGTCCAGCTGGAGCCGCTGGGCCC
  ATCGGAATTCCAGGGAGACCTGGGCCCCAGGGACCCCCAGGGCCGGCAGGAGAGAAAGGG
  GCTCCTGGCGAGAAAGGCCCACAAGGCCCAGCTGGCCGAGACGGTCTCCAGGGGCCTGTG
  GGGCTCCCGGGTCCAGCTGGCCCTGTGGGTCCCCCTGGAGAAGACGGAGATAAGGGAGAG
  ATCGGGGAGCCGGGGCAGAAAGGAAGCAAGGGGGACAAAGGAGAACAGGGTCCTCCTGGG
  CCTACAGGTCCTCAAGGCCCCATCGGACAGCCAGGCCCCTCTGGAGCTGACGGCGAGCCG
  GGGCCTCGGGGCCAGCAGGGCCTTTTCGGGCAGAAAGGTGATGAAGGTCCCAGAGGCTTT
  CCTGGACCCCCTGGGCCAGTGGGGCTGCAGGGTTTGCCAGGACCTCCAGGCGAGAAGGGT
  GAGACAGGAGACGTGGGCCAGATGGGCCCCCCGGGTCCCCCTGGCCCCCGAGGACCCTCC
  GGAGCTCCAGGTGCTGATGGCCCACAAGGTCCCCCAGGTGGAATAGGAAACCCTGGTGCA
  GTGGGAGAGAAGGGCGAGCCTGGCGAAGCAGGTGAGCCTGGCCTTCCGGGAGAAGGCGGC
  CCCCCGGGACCCAAAGGAGAAAGGGGAGAGAAGGGCGAGTCAGGCCCTTCAGGTGCTGCC
  GGACCCCCTGGACCCAAAGGCCCTCCCGGAGATGATGGTCCCAAAGGCAGCCCTGGCCCA
  GTGGGTTTTCCTGGAGATCCTGGCCCCCCCGGAGAGCCTGGCCCCGCGGGTCAAGATGGT
  CCCCCTGGTGACAAAGGAGATGATGGTGAACCCGGGCAGACGGGATCCCCCGGCCCTACT
  GGTGAACCAGGTCCATCGGGGCCTCCAGGAAAAAGGGGTCCCCCAGGCCCCGCAGGCCCC
  GAAGGCAGACAGGGAGAGAAAGGGGCCAAGGGAGAAGCCGGCTTGGAAGGCCCTCCTGGG
  AAGACTGGCCCCATCGGCCCCCAGGGGGCCCCTGGGAAGCCCGGACCGGATGGCCTTCGA
  GGGATCCCTGGCCCTGTGGGAGAACAAGGTCTCCCAGGATCCCCAGGCCCGGACGGTCCC
  CCCGGCCCCATGGGTCCCCCAGGACTTCCCGGCCTCAAAGGAGATTCTGGTCCCAAAGGT
  GAAAAGGGTCATCCAGGCCTGATCGGGCTCATCGGTCCTCCGGGTGAACAGGGTGAGAAG
  GGCGACCGTGGTCTCCCTGGCCCCCAGGGCTCCTCCGGTCCTAAGGGAGAACAGGGTATC
  ACTGGTCCTTCTGGCCCGATTGGGCCTCCTGGGCCCCCTGGCCTGCCGGGTCCGCCTGGT
  CCAAAAGGTGCTAAGGGCTCCTCGGGTCCAACTGGCCCGAAGGGTGAGGCAGGCCACCCA
  GGACCCCCAGGCCCCCCGGGCCCCCCGGGAGAGGTCATCCAGCCCCTGCCAATCCAGGCA
  TCCAGGACGCGGCGGAACATCGACGCCAGCCAGCTGCTGGACGACGGGAATGGCGAGAAC
  TACGTGGACTACGCGGACGGCATGGAAGAGATCTTCGGCTCTCTCAACTCTCTGAAGCTG
  GAGATTGAGCAGATGAAACGGCCCCTGGGCACGCAGCAGAACCCCGCCCGCACCTGCAAG
  GACCTGCAGCTCTGCCACCCCGACTTCCCAGATGGTGAATACTGGGTCGATCCTAACCAA
  GGATGCTCCAGGGATTCCTTCAAGGTTTACTGCAACTTCACAGCCGGGGGGTCGACATGC
  GTCTTCCCTGACAAGAAGTCCGAAGGGGCCAGAATCACTTCTTGGCCCAAAGAAAACCCG
  GGCTCCTGGTTCAGTGAATTCAAGCGTGGGAAACTGCTCTCCTATGTGGACGCCGAGGGC
  AACCCTGTGGGTGTGGTACAGATGACCTTCCTGCGGCTGCTGAGCGCCTCTGCCCACCAG
  AACGTCACCTACCACTGCTACCAGTCAGTGGCCTGGCAGGACGCAGCCACGGGCAGCTAC
  GACAAGGCCCTCCGCTTCCTGGGCTCCAACGACGAGGAGATGTCCTATGACAACAACCCC
  TACATCCGCGCCCTGGTGGACGGCTGTGCTACCAAGAAAGGCTACCAGAAGACGGTTCTG
  GAGATCGACACCCCCAAAGTGGAGCAGGTGCCCATCGTGGACATCATGTTCAATGACTTC
  GGTGAAGCGTCACAGAAATTTGGATTTGAAGTGGGGCCGGCTTGCTTCATGGGCTAG
  
• Restriction Sites: Please inquire
• ACCN: NM_000093
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000093.2, NP_000084.2
• RefSeq Size: 6496 bp
• RefSeq ORF: 5517 bp
• Locus ID: 1289
• Cytogenetics: 9q34.3
• Protein Families: Druggable Genome
• Protein Pathways: ECM-receptor interaction, Focal adhesion
• Gene Summary: 'This gene encodes an alpha chain for one of the low abundance fibrillar collagens. Fibrillar collagen molecules are trimers that can be composed of one or more types of alpha chains. Type V collagen is found in tissues containing type I collagen and appears to regulate the assembly of heterotypic fibers composed of both type I and type V collagen. This gene product is closely related to type XI collagen and it is possible that the collagen chains of types V and XI constitute a single collagen type with tissue-specific chain combinations. The encoded procollagen protein occurs commonly as the heterotrimer pro-alpha1(V)-pro-alpha1(V)-pro-alpha2(V). Mutations in this gene are associated with Ehlers-Danlos syndrome, types I and II. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, May 2013]'
  Transcript Variant: This variant (1) encodes isoform 1.