FUT3 (NM_000149) Human Untagged Clone

CAT#: SC300017

FUT3 (untagged)-Human fucosyltransferase 3 (galactoside 3(4)-L-fucosyltransferase, Lewis blood group) (FUT3), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: FUT3
• Synonyms: CD174; FT3B; FucT-III; LE; Les
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000149 edited
  GAGAAAGCAGGCAACCACCATGTCATTTGAAAACAGTTTCATCGGGATATAATTCGCAAC
  CCATACAGTGAATCCATTTAAGATACTCTGACCCATGGATCCCCTGGGTGCAGCCAAGCC
  ACAATGGCCATGGCGCCGCTGTCTGGCCGCACTGCTATTTCAGCTGCTGGTGGCTGTGTG
  TTTCTTCTCCTACCTGCGTGTGTCCCGAGACGATGCCACTGGATCCCCTAGGGCTCCCAG
  TGGGTCCTCCCGACAGGACACCACTCCCACCCGCCCCACCCTCCTGATCCTGCTATGGAC
  ATGGCCTTTCCACATCCCTGTGGCTCTGTCCCGCTGTTCAGAGATGGTGCCCGGCACAGC
  CGACTGCCACATCACTGCCGACCGCAAGGTGTACCCACAGGCAGACACGGTCATCGTGCA
  CCACTGGGATATCATGTCCAACCCTAAGTCACGCCTCCCACCTTCCCCGAGGCCGCAGGG
  GCAGCGCTGGATCTGGTTCAACTTGGAGCCACCCCCTAACTGCCAGCACCTGGAAGCCCT
  GGACAGATACTTCAATCTCACCATGTCCTACCGCAGCGACTCCGACATCTTCACGCCCTA
  CGGCTGGCTGGAGCCGTGGTCCGGCCAGCCTGCCCACCCACCGCTCAACCTCTCGGCCAA
  GACCGAGCTGGTGGCCTGGGCGGTGTCCAACTGGAAGCCGGACTCAGCCAGGGTGCGCTA
  CTACCAGAGCCTGCAGGCTCATCTCAAGGTGGACGTGTACGGACGCTCCCACAAGCCCCT
  GCCCAAGGGGACCATGATGGAGACGCTGTCCCGGTACAAGTTCTACCTGGCCTTCGAGAA
  CTCCTTGCACCCCGACTACATCACCGAGAAGCTGTGGAGGAACGCCCTGGAGGCCTGGGC
  CGTGCCCGTGGTGCTGGGCCCCAGCAGAAGCAACTACGAGAGGTTCCTGCCACCCGACGC
  CTTCATCCACGTGGACGACTTCCAGAGCCCCAAGGACCTGGCCCGGTACCTGCAGGAGCT
  GGACAAGGACCACGCCCGCTACCTGAGCTACTTTCGCTGGCGGGAGACGCTGCGGCCTCG
  CTCCTTCAGCTGGGCACTGGATTTCTGCAAGGCCTGCTGGAAACTGCAGCAGGAATCCAG
  GTACCAGACGGTGCGCAGCATAGCGGCTTGGTTCACCTGAGAGGCCGGCATGGTGCCTGG
  GCTGCCGGGAACCTCATCTGCCTGGGGCCTCACCTGCTGGAGTCCTTTGTGGCCAACCCT
  CTCTCTTACCTGGGACCTCACACGCTGGGCTTCACGGCTGCCAGGAGCCTCTCCCCTCCA
  GAAGACTTGCCTGCTGGGGACCTCGCCTGCTGGGGACCTCGCCTGTTGGGGACCTCACCT
  GCTGGGGACCTCACCTGCTGGGGACCTTGGCTGCTGGAGGCTGCACCTACTGAGGATGTC
  GGCGGTCGGGGACTTTACCTGCTGGGACCTGCTCCCAGAGACCTTGCCACACTGAATCTC
  ACCTGCTGGGGACCTCACCCTGGAGGGCCCTGGGCCCTGGGGAACTGGCTTACTTGGGGC
  CCCACCCGGGAGTGATGGTTCTGGCTGATTTGTTTGTGATGTTGTTAGCCGCCTGTGAGG
  GGTGCAGAGAGATCATCACGGCACGGTTTCCAGATGTAATACTGCAAGGAAAAAAAAAAA
  AAAAAAAAAAAAAA
  
• Restriction Sites: Please inquire
• ACCN: NM_000149
• Insert Size: 1700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The ORF of this clone has been fully sequenced and found to be a perfect match to NM_000149.1.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000149.1, NP_000140.1
• RefSeq Size: 2583 bp
• RefSeq ORF: 1086 bp
• Locus ID: 2525
• Cytogenetics: 19p13.3
• Protein Pathways: Glycosphingolipid biosynthesis - lacto and neolacto series, Metabolic pathways
• Gene Summary: 'The Lewis histo-blood group system comprises a set of fucosylated glycosphingolipids that are synthesized by exocrine epithelial cells and circulate in body fluids. The glycosphingolipids function in embryogenesis, tissue differentiation, tumor metastasis, inflammation, and bacterial adhesion. They are secondarily absorbed to red blood cells giving rise to their Lewis phenotype. This gene is a member of the fucosyltransferase family, which catalyzes the addition of fucose to precursor polysaccharides in the last step of Lewis antigen biosynthesis. It encodes an enzyme with alpha(1,3)-fucosyltransferase and alpha(1,4)-fucosyltransferase activities. Mutations in this gene are responsible for the majority of Lewis antigen-negative phenotypes. Differences in the expression of this gene are associated with host susceptibility to viral infection. [provided by RefSeq, Aug 2020]'
  Transcript Variant: This variant (1), also known as minor I, represents the longest transcript. Variants 1, 2, 3, and 4 encode the same protein.